Meloidogyne incognita: molecular cloning and characterization of a cDNA encoding a cathepsin D-like aspartic proteinase
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UCB |
Texto Completo: | http://twingo.ucb.br:8080/jspui/handle/10869/482 https://repositorio.ucb.br:9443/jspui/handle/123456789/7686 |
Resumo: | Herein we describe the cloning and characterization of a cDNA encoding an aspartic proteinase from the root-knot nematode Meloidogyne incognita. Using PCR techniques, a 1471-bp cDNA fragment encoding a cathepsin D-like (Mi-asp1) transcript was isolated from second- stage larvae mRNA. Its predicted amino acid sequence comprises a pro-region of 71 amino acid residues and a mature protease of 378 amino acid residues with a predicted molecular mass of 41.502 kDa. Protein sequence comparisons of Mi-asp1 with GenBank ™ (DQ360827) sequences showed 59–71% identity with nematode- specific cathepsin D-like aspartic proteinases. Southern blot analysis, RT-PCR amplification and EST mining suggest the existence of a developmentally expressed gene family encoding aspartic proteinases in M. incognita. Mi-asp1 may represent a potential target for molecular intervention for the purposes of plant–parasitic nematode control. |
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Fragoso, Rodrigo da RochaLourenço, Isabela TristanBatista, João Aguiar NogueiraOliveira-Neto, Osmundo BrilhanteSilva, Maria Cristina MattarRocha, Thales LimaRocha, Thales LimaCoutinho, Marise VenturaGrossi-de-Sa, Maria Fátima2016-10-10T03:52:21Z2016-10-10T03:52:21Z2009FRAGOSO, Rodrigo da Rocha, et al. Meloidogyne incognita: molecular cloning and characterization of a cDNA encoding a cathepsin D-like aspartic proteinase. Experimental Parasitology, v. 121, p. 115–123, 2009.http://twingo.ucb.br:8080/jspui/handle/10869/482https://repositorio.ucb.br:9443/jspui/handle/123456789/7686Herein we describe the cloning and characterization of a cDNA encoding an aspartic proteinase from the root-knot nematode Meloidogyne incognita. Using PCR techniques, a 1471-bp cDNA fragment encoding a cathepsin D-like (Mi-asp1) transcript was isolated from second- stage larvae mRNA. Its predicted amino acid sequence comprises a pro-region of 71 amino acid residues and a mature protease of 378 amino acid residues with a predicted molecular mass of 41.502 kDa. Protein sequence comparisons of Mi-asp1 with GenBank ™ (DQ360827) sequences showed 59–71% identity with nematode- specific cathepsin D-like aspartic proteinases. Southern blot analysis, RT-PCR amplification and EST mining suggest the existence of a developmentally expressed gene family encoding aspartic proteinases in M. incognita. Mi-asp1 may represent a potential target for molecular intervention for the purposes of plant–parasitic nematode control.Made available in DSpace on 2016-10-10T03:52:21Z (GMT). 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dc.title.pt_BR.fl_str_mv |
Meloidogyne incognita: molecular cloning and characterization of a cDNA encoding a cathepsin D-like aspartic proteinase |
title |
Meloidogyne incognita: molecular cloning and characterization of a cDNA encoding a cathepsin D-like aspartic proteinase |
spellingShingle |
Meloidogyne incognita: molecular cloning and characterization of a cDNA encoding a cathepsin D-like aspartic proteinase Fragoso, Rodrigo da Rocha cDNA cloning Meloidogyne Nematode Aspartic proteinase Cathepsin D RKM-Root-knot nematode CN-cyst nematode L2 - second-stage larva or juvenile AP- aspartic proteinase RACE- rapid amplification EST, expressed sequence tag RT-PCR- reverse transcription and polymerase chain reaction ORF- open read frame |
title_short |
Meloidogyne incognita: molecular cloning and characterization of a cDNA encoding a cathepsin D-like aspartic proteinase |
title_full |
Meloidogyne incognita: molecular cloning and characterization of a cDNA encoding a cathepsin D-like aspartic proteinase |
title_fullStr |
Meloidogyne incognita: molecular cloning and characterization of a cDNA encoding a cathepsin D-like aspartic proteinase |
title_full_unstemmed |
Meloidogyne incognita: molecular cloning and characterization of a cDNA encoding a cathepsin D-like aspartic proteinase |
title_sort |
Meloidogyne incognita: molecular cloning and characterization of a cDNA encoding a cathepsin D-like aspartic proteinase |
author |
Fragoso, Rodrigo da Rocha |
author_facet |
Fragoso, Rodrigo da Rocha Lourenço, Isabela Tristan Batista, João Aguiar Nogueira Oliveira-Neto, Osmundo Brilhante Silva, Maria Cristina Mattar Rocha, Thales Lima Coutinho, Marise Ventura Grossi-de-Sa, Maria Fátima |
author_role |
author |
author2 |
Lourenço, Isabela Tristan Batista, João Aguiar Nogueira Oliveira-Neto, Osmundo Brilhante Silva, Maria Cristina Mattar Rocha, Thales Lima Coutinho, Marise Ventura Grossi-de-Sa, Maria Fátima |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Fragoso, Rodrigo da Rocha Lourenço, Isabela Tristan Batista, João Aguiar Nogueira Oliveira-Neto, Osmundo Brilhante Silva, Maria Cristina Mattar Rocha, Thales Lima Rocha, Thales Lima Coutinho, Marise Ventura Grossi-de-Sa, Maria Fátima |
dc.subject.por.fl_str_mv |
cDNA cloning Meloidogyne Nematode Aspartic proteinase Cathepsin D RKM-Root-knot nematode CN-cyst nematode L2 - second-stage larva or juvenile AP- aspartic proteinase RACE- rapid amplification EST, expressed sequence tag RT-PCR- reverse transcription and polymerase chain reaction ORF- open read frame |
topic |
cDNA cloning Meloidogyne Nematode Aspartic proteinase Cathepsin D RKM-Root-knot nematode CN-cyst nematode L2 - second-stage larva or juvenile AP- aspartic proteinase RACE- rapid amplification EST, expressed sequence tag RT-PCR- reverse transcription and polymerase chain reaction ORF- open read frame |
dc.description.abstract.por.fl_txt_mv |
Herein we describe the cloning and characterization of a cDNA encoding an aspartic proteinase from the root-knot nematode Meloidogyne incognita. Using PCR techniques, a 1471-bp cDNA fragment encoding a cathepsin D-like (Mi-asp1) transcript was isolated from second- stage larvae mRNA. Its predicted amino acid sequence comprises a pro-region of 71 amino acid residues and a mature protease of 378 amino acid residues with a predicted molecular mass of 41.502 kDa. Protein sequence comparisons of Mi-asp1 with GenBank ™ (DQ360827) sequences showed 59–71% identity with nematode- specific cathepsin D-like aspartic proteinases. Southern blot analysis, RT-PCR amplification and EST mining suggest the existence of a developmentally expressed gene family encoding aspartic proteinases in M. incognita. Mi-asp1 may represent a potential target for molecular intervention for the purposes of plant–parasitic nematode control. |
dc.description.version.pt_BR.fl_txt_mv |
Sim |
dc.description.status.pt_BR.fl_txt_mv |
Publicado |
description |
Herein we describe the cloning and characterization of a cDNA encoding an aspartic proteinase from the root-knot nematode Meloidogyne incognita. Using PCR techniques, a 1471-bp cDNA fragment encoding a cathepsin D-like (Mi-asp1) transcript was isolated from second- stage larvae mRNA. Its predicted amino acid sequence comprises a pro-region of 71 amino acid residues and a mature protease of 378 amino acid residues with a predicted molecular mass of 41.502 kDa. Protein sequence comparisons of Mi-asp1 with GenBank ™ (DQ360827) sequences showed 59–71% identity with nematode- specific cathepsin D-like aspartic proteinases. Southern blot analysis, RT-PCR amplification and EST mining suggest the existence of a developmentally expressed gene family encoding aspartic proteinases in M. incognita. Mi-asp1 may represent a potential target for molecular intervention for the purposes of plant–parasitic nematode control. |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009 |
dc.date.accessioned.fl_str_mv |
2016-10-10T03:52:21Z |
dc.date.available.fl_str_mv |
2016-10-10T03:52:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
status_str |
publishedVersion |
format |
article |
dc.identifier.citation.fl_str_mv |
FRAGOSO, Rodrigo da Rocha, et al. Meloidogyne incognita: molecular cloning and characterization of a cDNA encoding a cathepsin D-like aspartic proteinase. Experimental Parasitology, v. 121, p. 115–123, 2009. |
dc.identifier.uri.fl_str_mv |
http://twingo.ucb.br:8080/jspui/handle/10869/482 https://repositorio.ucb.br:9443/jspui/handle/123456789/7686 |
identifier_str_mv |
FRAGOSO, Rodrigo da Rocha, et al. Meloidogyne incognita: molecular cloning and characterization of a cDNA encoding a cathepsin D-like aspartic proteinase. Experimental Parasitology, v. 121, p. 115–123, 2009. |
url |
http://twingo.ucb.br:8080/jspui/handle/10869/482 https://repositorio.ucb.br:9443/jspui/handle/123456789/7686 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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Texto |
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UCB |
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UCB |
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Repositório Institucional da UCB |
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Repositório Institucional da UCB |
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