Identification of a novel bean a-amylase inhibitor with chitinolytic activity

Detalhes bibliográficos
Autor(a) principal: Dayler, Charles S.A.
Data de Publicação: 2005
Outros Autores: Mendes, Paulo A.M., Prates, Maura V., Bloch Júnior, Carlos, Franco, Octavio Luiz, Grossi-de-Sá, Maria Fátima
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UCB
Texto Completo: http://twingo.ucb.br:8080/jspui/handle/10869/485
https://repositorio.ucb.br:9443/jspui/handle/123456789/7746
Resumo: Zabrotes subfasciatus is a devastating starch-dependent storage bean pest. In this study, we attempted to identify novel a-amylase inhibitors from wild bean seeds, with efficiency toward pest a-amylases. An inhibitor named Phaseolus vulgaris chitinolytic a-amylase inhibitor (PvCAI) was purified and mass spectrometry analyses showed a protein with 33330 Da with the ability to form dimers. Purified PvCAI showed significant inhibitory activity against larval Z. subfasciatus a-amylases with no activity against mammalian enzymes. N-terminal sequence analyses showed an unexpected high identity to plant chitinases from the glycoside hydrolase family 18. Furthermore, their chitinolytic activity was also detected. Our data provides compelling evidence that PvCAI also possessed chitinolytic activity, indicating the emergence of a novel a-amylase inhibitor class.
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spelling Dayler, Charles S.A.Mendes, Paulo A.M.Prates, Maura V.Bloch Júnior, CarlosFranco, Octavio LuizGrossi-de-Sá, Maria Fátima2016-10-10T03:52:32Z2016-10-10T03:52:32Z2005DAYLER, Charles S. A. et al. Identification of a novel bean a-amylase inhibitor with chitinolytic activity. FEBS Letters, v.579, p. 5616–5620, 2005.http://twingo.ucb.br:8080/jspui/handle/10869/485https://repositorio.ucb.br:9443/jspui/handle/123456789/7746Zabrotes subfasciatus is a devastating starch-dependent storage bean pest. In this study, we attempted to identify novel a-amylase inhibitors from wild bean seeds, with efficiency toward pest a-amylases. An inhibitor named Phaseolus vulgaris chitinolytic a-amylase inhibitor (PvCAI) was purified and mass spectrometry analyses showed a protein with 33330 Da with the ability to form dimers. Purified PvCAI showed significant inhibitory activity against larval Z. subfasciatus a-amylases with no activity against mammalian enzymes. N-terminal sequence analyses showed an unexpected high identity to plant chitinases from the glycoside hydrolase family 18. Furthermore, their chitinolytic activity was also detected. Our data provides compelling evidence that PvCAI also possessed chitinolytic activity, indicating the emergence of a novel a-amylase inhibitor class.Made available in DSpace on 2016-10-10T03:52:32Z (GMT). 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dc.title.pt_BR.fl_str_mv Identification of a novel bean a-amylase inhibitor with chitinolytic activity
title Identification of a novel bean a-amylase inhibitor with chitinolytic activity
spellingShingle Identification of a novel bean a-amylase inhibitor with chitinolytic activity
Dayler, Charles S.A.
A-Amylase Inhibitor
Chitinase
Zabrotes subfasciatus
Bruchids
Phaseolus vulgaris
title_short Identification of a novel bean a-amylase inhibitor with chitinolytic activity
title_full Identification of a novel bean a-amylase inhibitor with chitinolytic activity
title_fullStr Identification of a novel bean a-amylase inhibitor with chitinolytic activity
title_full_unstemmed Identification of a novel bean a-amylase inhibitor with chitinolytic activity
title_sort Identification of a novel bean a-amylase inhibitor with chitinolytic activity
author Dayler, Charles S.A.
author_facet Dayler, Charles S.A.
Mendes, Paulo A.M.
Prates, Maura V.
Bloch Júnior, Carlos
Franco, Octavio Luiz
Grossi-de-Sá, Maria Fátima
author_role author
author2 Mendes, Paulo A.M.
Prates, Maura V.
Bloch Júnior, Carlos
Franco, Octavio Luiz
Grossi-de-Sá, Maria Fátima
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Dayler, Charles S.A.
Mendes, Paulo A.M.
Prates, Maura V.
Bloch Júnior, Carlos
Franco, Octavio Luiz
Grossi-de-Sá, Maria Fátima
dc.subject.por.fl_str_mv A-Amylase Inhibitor
Chitinase
Zabrotes subfasciatus
Bruchids
Phaseolus vulgaris
topic A-Amylase Inhibitor
Chitinase
Zabrotes subfasciatus
Bruchids
Phaseolus vulgaris
dc.description.abstract.por.fl_txt_mv Zabrotes subfasciatus is a devastating starch-dependent storage bean pest. In this study, we attempted to identify novel a-amylase inhibitors from wild bean seeds, with efficiency toward pest a-amylases. An inhibitor named Phaseolus vulgaris chitinolytic a-amylase inhibitor (PvCAI) was purified and mass spectrometry analyses showed a protein with 33330 Da with the ability to form dimers. Purified PvCAI showed significant inhibitory activity against larval Z. subfasciatus a-amylases with no activity against mammalian enzymes. N-terminal sequence analyses showed an unexpected high identity to plant chitinases from the glycoside hydrolase family 18. Furthermore, their chitinolytic activity was also detected. Our data provides compelling evidence that PvCAI also possessed chitinolytic activity, indicating the emergence of a novel a-amylase inhibitor class.
dc.description.version.pt_BR.fl_txt_mv Sim
dc.description.status.pt_BR.fl_txt_mv Publicado
description Zabrotes subfasciatus is a devastating starch-dependent storage bean pest. In this study, we attempted to identify novel a-amylase inhibitors from wild bean seeds, with efficiency toward pest a-amylases. An inhibitor named Phaseolus vulgaris chitinolytic a-amylase inhibitor (PvCAI) was purified and mass spectrometry analyses showed a protein with 33330 Da with the ability to form dimers. Purified PvCAI showed significant inhibitory activity against larval Z. subfasciatus a-amylases with no activity against mammalian enzymes. N-terminal sequence analyses showed an unexpected high identity to plant chitinases from the glycoside hydrolase family 18. Furthermore, their chitinolytic activity was also detected. Our data provides compelling evidence that PvCAI also possessed chitinolytic activity, indicating the emergence of a novel a-amylase inhibitor class.
publishDate 2005
dc.date.issued.fl_str_mv 2005
dc.date.accessioned.fl_str_mv 2016-10-10T03:52:32Z
dc.date.available.fl_str_mv 2016-10-10T03:52:32Z
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dc.identifier.citation.fl_str_mv DAYLER, Charles S. A. et al. Identification of a novel bean a-amylase inhibitor with chitinolytic activity. FEBS Letters, v.579, p. 5616–5620, 2005.
dc.identifier.uri.fl_str_mv http://twingo.ucb.br:8080/jspui/handle/10869/485
https://repositorio.ucb.br:9443/jspui/handle/123456789/7746
identifier_str_mv DAYLER, Charles S. A. et al. Identification of a novel bean a-amylase inhibitor with chitinolytic activity. FEBS Letters, v.579, p. 5616–5620, 2005.
url http://twingo.ucb.br:8080/jspui/handle/10869/485
https://repositorio.ucb.br:9443/jspui/handle/123456789/7746
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