Characterization of Aspergillus flavus strains from Brazilian Brazil nuts and cashew by RAPD and ribosomal DNA Analysis
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UCB |
Texto Completo: | http://twingo.ucb.br:8080/jspui/handle/10869/699 https://repositorio.ucb.br:9443/jspui/handle/123456789/7931 |
Resumo: | Aims: The aim of this study was to determine the genetic variability in Aspergillus flavus populations from Brazil nut and cashew and develop a polymerase chain reaction (PCR) detection method. Methods and Results: Chomatography analysis of 48 isolates identified 36 as aflatoxigenic (75%). One hundred and forty-one DNA bands were generated with 11 random amplified polymorphic DNA (RAPD) primers and analysed via unweighted pair group analysis, using arithmetic means (UPGMA). Isolates grouped according to host, with differentiation of those from A. occidentale also according to geographical origin. Aspergillus flavus-specific PCR primers ASPITSF2 and ASPITSR3 were designed from ribosomal DNA internal transcribed spacers (ITS 1 and 2), and an internal amplification control was developed, to prevent false negative results. Specificity to only A. flavus was confirmed against DNA from additional aspergilli and other fungi. Conclusions: RAPD-based characterization differentiated isolates according to plant host. The PCR primer pair developed showed specificity to A. flavus, with a detection limit of 10 fg. Significance and Impact of the Study: Genetic variability observed in A. flavus isolates from two Brazilian agroecosystems suggested reproductive isolation. The PCR detection method developed for A. flavus represents progress towards multiplex PCR detection of aflatoxigenic and nonaflatoxigenic strains in Hazard Analysis Critical Control Point systems. |
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Midorikawa, Gláucia Emy OkidaPinheiro, Maria dos Reis RodriguesSantos, Bruna Vidigal dosArruda, Maricilia Conceição Cardoso deCosta, Felipe F.Pappas Júnior, Georgios JoannisRibeiro, Simone da GraçaFreire, FranciscoMiller, Robert Neil Gerard2016-10-10T03:53:08Z2016-10-10T03:53:08Z2008MIDORIKAWA, Gláucia Emy Okida et al. Characterization of Aspergillus flavus strains from Brazilian Brazil nut and cashew by RAPD and ribosomal DNA analysis. Letters in Applied Microbiology, v. 47, p. 12-18, 2008.http://twingo.ucb.br:8080/jspui/handle/10869/699https://repositorio.ucb.br:9443/jspui/handle/123456789/7931Aims: The aim of this study was to determine the genetic variability in Aspergillus flavus populations from Brazil nut and cashew and develop a polymerase chain reaction (PCR) detection method. Methods and Results: Chomatography analysis of 48 isolates identified 36 as aflatoxigenic (75%). One hundred and forty-one DNA bands were generated with 11 random amplified polymorphic DNA (RAPD) primers and analysed via unweighted pair group analysis, using arithmetic means (UPGMA). Isolates grouped according to host, with differentiation of those from A. occidentale also according to geographical origin. Aspergillus flavus-specific PCR primers ASPITSF2 and ASPITSR3 were designed from ribosomal DNA internal transcribed spacers (ITS 1 and 2), and an internal amplification control was developed, to prevent false negative results. Specificity to only A. flavus was confirmed against DNA from additional aspergilli and other fungi. Conclusions: RAPD-based characterization differentiated isolates according to plant host. The PCR primer pair developed showed specificity to A. flavus, with a detection limit of 10 fg. Significance and Impact of the Study: Genetic variability observed in A. flavus isolates from two Brazilian agroecosystems suggested reproductive isolation. The PCR detection method developed for A. flavus represents progress towards multiplex PCR detection of aflatoxigenic and nonaflatoxigenic strains in Hazard Analysis Critical Control Point systems.Made available in DSpace on 2016-10-10T03:53:08Z (GMT). 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dc.title.pt_BR.fl_str_mv |
Characterization of Aspergillus flavus strains from Brazilian Brazil nuts and cashew by RAPD and ribosomal DNA Analysis |
title |
Characterization of Aspergillus flavus strains from Brazilian Brazil nuts and cashew by RAPD and ribosomal DNA Analysis |
spellingShingle |
Characterization of Aspergillus flavus strains from Brazilian Brazil nuts and cashew by RAPD and ribosomal DNA Analysis Midorikawa, Gláucia Emy Okida Aspergillus flavus Detection Internal amplification control Mycotoxins PCR RAPD rDNA ITS |
title_short |
Characterization of Aspergillus flavus strains from Brazilian Brazil nuts and cashew by RAPD and ribosomal DNA Analysis |
title_full |
Characterization of Aspergillus flavus strains from Brazilian Brazil nuts and cashew by RAPD and ribosomal DNA Analysis |
title_fullStr |
Characterization of Aspergillus flavus strains from Brazilian Brazil nuts and cashew by RAPD and ribosomal DNA Analysis |
title_full_unstemmed |
Characterization of Aspergillus flavus strains from Brazilian Brazil nuts and cashew by RAPD and ribosomal DNA Analysis |
title_sort |
Characterization of Aspergillus flavus strains from Brazilian Brazil nuts and cashew by RAPD and ribosomal DNA Analysis |
author |
Midorikawa, Gláucia Emy Okida |
author_facet |
Midorikawa, Gláucia Emy Okida Pinheiro, Maria dos Reis Rodrigues Santos, Bruna Vidigal dos Arruda, Maricilia Conceição Cardoso de Costa, Felipe F. Pappas Júnior, Georgios Joannis Ribeiro, Simone da Graça Freire, Francisco Miller, Robert Neil Gerard |
author_role |
author |
author2 |
Pinheiro, Maria dos Reis Rodrigues Santos, Bruna Vidigal dos Arruda, Maricilia Conceição Cardoso de Costa, Felipe F. Pappas Júnior, Georgios Joannis Ribeiro, Simone da Graça Freire, Francisco Miller, Robert Neil Gerard |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Midorikawa, Gláucia Emy Okida Pinheiro, Maria dos Reis Rodrigues Santos, Bruna Vidigal dos Arruda, Maricilia Conceição Cardoso de Costa, Felipe F. Pappas Júnior, Georgios Joannis Ribeiro, Simone da Graça Freire, Francisco Miller, Robert Neil Gerard |
dc.subject.por.fl_str_mv |
Aspergillus flavus Detection Internal amplification control Mycotoxins PCR RAPD rDNA ITS |
topic |
Aspergillus flavus Detection Internal amplification control Mycotoxins PCR RAPD rDNA ITS |
dc.description.abstract.por.fl_txt_mv |
Aims: The aim of this study was to determine the genetic variability in Aspergillus flavus populations from Brazil nut and cashew and develop a polymerase chain reaction (PCR) detection method. Methods and Results: Chomatography analysis of 48 isolates identified 36 as aflatoxigenic (75%). One hundred and forty-one DNA bands were generated with 11 random amplified polymorphic DNA (RAPD) primers and analysed via unweighted pair group analysis, using arithmetic means (UPGMA). Isolates grouped according to host, with differentiation of those from A. occidentale also according to geographical origin. Aspergillus flavus-specific PCR primers ASPITSF2 and ASPITSR3 were designed from ribosomal DNA internal transcribed spacers (ITS 1 and 2), and an internal amplification control was developed, to prevent false negative results. Specificity to only A. flavus was confirmed against DNA from additional aspergilli and other fungi. Conclusions: RAPD-based characterization differentiated isolates according to plant host. The PCR primer pair developed showed specificity to A. flavus, with a detection limit of 10 fg. Significance and Impact of the Study: Genetic variability observed in A. flavus isolates from two Brazilian agroecosystems suggested reproductive isolation. The PCR detection method developed for A. flavus represents progress towards multiplex PCR detection of aflatoxigenic and nonaflatoxigenic strains in Hazard Analysis Critical Control Point systems. |
dc.description.status.pt_BR.fl_txt_mv |
Publicado |
description |
Aims: The aim of this study was to determine the genetic variability in Aspergillus flavus populations from Brazil nut and cashew and develop a polymerase chain reaction (PCR) detection method. Methods and Results: Chomatography analysis of 48 isolates identified 36 as aflatoxigenic (75%). One hundred and forty-one DNA bands were generated with 11 random amplified polymorphic DNA (RAPD) primers and analysed via unweighted pair group analysis, using arithmetic means (UPGMA). Isolates grouped according to host, with differentiation of those from A. occidentale also according to geographical origin. Aspergillus flavus-specific PCR primers ASPITSF2 and ASPITSR3 were designed from ribosomal DNA internal transcribed spacers (ITS 1 and 2), and an internal amplification control was developed, to prevent false negative results. Specificity to only A. flavus was confirmed against DNA from additional aspergilli and other fungi. Conclusions: RAPD-based characterization differentiated isolates according to plant host. The PCR primer pair developed showed specificity to A. flavus, with a detection limit of 10 fg. Significance and Impact of the Study: Genetic variability observed in A. flavus isolates from two Brazilian agroecosystems suggested reproductive isolation. The PCR detection method developed for A. flavus represents progress towards multiplex PCR detection of aflatoxigenic and nonaflatoxigenic strains in Hazard Analysis Critical Control Point systems. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008 |
dc.date.accessioned.fl_str_mv |
2016-10-10T03:53:08Z |
dc.date.available.fl_str_mv |
2016-10-10T03:53:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
status_str |
publishedVersion |
format |
article |
dc.identifier.citation.fl_str_mv |
MIDORIKAWA, Gláucia Emy Okida et al. Characterization of Aspergillus flavus strains from Brazilian Brazil nut and cashew by RAPD and ribosomal DNA analysis. Letters in Applied Microbiology, v. 47, p. 12-18, 2008. |
dc.identifier.uri.fl_str_mv |
http://twingo.ucb.br:8080/jspui/handle/10869/699 https://repositorio.ucb.br:9443/jspui/handle/123456789/7931 |
identifier_str_mv |
MIDORIKAWA, Gláucia Emy Okida et al. Characterization of Aspergillus flavus strains from Brazilian Brazil nut and cashew by RAPD and ribosomal DNA analysis. Letters in Applied Microbiology, v. 47, p. 12-18, 2008. |
url |
http://twingo.ucb.br:8080/jspui/handle/10869/699 https://repositorio.ucb.br:9443/jspui/handle/123456789/7931 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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openAccess |
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UCB |
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UCB |
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Repositório Institucional da UCB |
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Repositório Institucional da UCB |
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