Characterization of Aspergillus flavus strains from Brazilian Brazil nuts and cashew by RAPD and ribosomal DNA Analysis

Detalhes bibliográficos
Autor(a) principal: Midorikawa, Gláucia Emy Okida
Data de Publicação: 2008
Outros Autores: Pinheiro, Maria dos Reis Rodrigues, Santos, Bruna Vidigal dos, Arruda, Maricilia Conceição Cardoso de, Costa, Felipe F., Pappas Júnior, Georgios Joannis, Ribeiro, Simone da Graça, Freire, Francisco, Miller, Robert Neil Gerard
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UCB
Texto Completo: http://twingo.ucb.br:8080/jspui/handle/10869/699
https://repositorio.ucb.br:9443/jspui/handle/123456789/7931
Resumo: Aims: The aim of this study was to determine the genetic variability in Aspergillus flavus populations from Brazil nut and cashew and develop a polymerase chain reaction (PCR) detection method. Methods and Results: Chomatography analysis of 48 isolates identified 36 as aflatoxigenic (75%). One hundred and forty-one DNA bands were generated with 11 random amplified polymorphic DNA (RAPD) primers and analysed via unweighted pair group analysis, using arithmetic means (UPGMA). Isolates grouped according to host, with differentiation of those from A. occidentale also according to geographical origin. Aspergillus flavus-specific PCR primers ASPITSF2 and ASPITSR3 were designed from ribosomal DNA internal transcribed spacers (ITS 1 and 2), and an internal amplification control was developed, to prevent false negative results. Specificity to only A. flavus was confirmed against DNA from additional aspergilli and other fungi. Conclusions: RAPD-based characterization differentiated isolates according to plant host. The PCR primer pair developed showed specificity to A. flavus, with a detection limit of 10 fg. Significance and Impact of the Study: Genetic variability observed in A. flavus isolates from two Brazilian agroecosystems suggested reproductive isolation. The PCR detection method developed for A. flavus represents progress towards multiplex PCR detection of aflatoxigenic and nonaflatoxigenic strains in Hazard Analysis Critical Control Point systems.
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spelling Midorikawa, Gláucia Emy OkidaPinheiro, Maria dos Reis RodriguesSantos, Bruna Vidigal dosArruda, Maricilia Conceição Cardoso deCosta, Felipe F.Pappas Júnior, Georgios JoannisRibeiro, Simone da GraçaFreire, FranciscoMiller, Robert Neil Gerard2016-10-10T03:53:08Z2016-10-10T03:53:08Z2008MIDORIKAWA, Gláucia Emy Okida et al. Characterization of Aspergillus flavus strains from Brazilian Brazil nut and cashew by RAPD and ribosomal DNA analysis. Letters in Applied Microbiology, v. 47, p. 12-18, 2008.http://twingo.ucb.br:8080/jspui/handle/10869/699https://repositorio.ucb.br:9443/jspui/handle/123456789/7931Aims: The aim of this study was to determine the genetic variability in Aspergillus flavus populations from Brazil nut and cashew and develop a polymerase chain reaction (PCR) detection method. Methods and Results: Chomatography analysis of 48 isolates identified 36 as aflatoxigenic (75%). One hundred and forty-one DNA bands were generated with 11 random amplified polymorphic DNA (RAPD) primers and analysed via unweighted pair group analysis, using arithmetic means (UPGMA). Isolates grouped according to host, with differentiation of those from A. occidentale also according to geographical origin. Aspergillus flavus-specific PCR primers ASPITSF2 and ASPITSR3 were designed from ribosomal DNA internal transcribed spacers (ITS 1 and 2), and an internal amplification control was developed, to prevent false negative results. Specificity to only A. flavus was confirmed against DNA from additional aspergilli and other fungi. Conclusions: RAPD-based characterization differentiated isolates according to plant host. The PCR primer pair developed showed specificity to A. flavus, with a detection limit of 10 fg. Significance and Impact of the Study: Genetic variability observed in A. flavus isolates from two Brazilian agroecosystems suggested reproductive isolation. The PCR detection method developed for A. flavus represents progress towards multiplex PCR detection of aflatoxigenic and nonaflatoxigenic strains in Hazard Analysis Critical Control Point systems.Made available in DSpace on 2016-10-10T03:53:08Z (GMT). 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dc.title.pt_BR.fl_str_mv Characterization of Aspergillus flavus strains from Brazilian Brazil nuts and cashew by RAPD and ribosomal DNA Analysis
title Characterization of Aspergillus flavus strains from Brazilian Brazil nuts and cashew by RAPD and ribosomal DNA Analysis
spellingShingle Characterization of Aspergillus flavus strains from Brazilian Brazil nuts and cashew by RAPD and ribosomal DNA Analysis
Midorikawa, Gláucia Emy Okida
Aspergillus flavus
Detection
Internal amplification control
Mycotoxins
PCR
RAPD
rDNA ITS
title_short Characterization of Aspergillus flavus strains from Brazilian Brazil nuts and cashew by RAPD and ribosomal DNA Analysis
title_full Characterization of Aspergillus flavus strains from Brazilian Brazil nuts and cashew by RAPD and ribosomal DNA Analysis
title_fullStr Characterization of Aspergillus flavus strains from Brazilian Brazil nuts and cashew by RAPD and ribosomal DNA Analysis
title_full_unstemmed Characterization of Aspergillus flavus strains from Brazilian Brazil nuts and cashew by RAPD and ribosomal DNA Analysis
title_sort Characterization of Aspergillus flavus strains from Brazilian Brazil nuts and cashew by RAPD and ribosomal DNA Analysis
author Midorikawa, Gláucia Emy Okida
author_facet Midorikawa, Gláucia Emy Okida
Pinheiro, Maria dos Reis Rodrigues
Santos, Bruna Vidigal dos
Arruda, Maricilia Conceição Cardoso de
Costa, Felipe F.
Pappas Júnior, Georgios Joannis
Ribeiro, Simone da Graça
Freire, Francisco
Miller, Robert Neil Gerard
author_role author
author2 Pinheiro, Maria dos Reis Rodrigues
Santos, Bruna Vidigal dos
Arruda, Maricilia Conceição Cardoso de
Costa, Felipe F.
Pappas Júnior, Georgios Joannis
Ribeiro, Simone da Graça
Freire, Francisco
Miller, Robert Neil Gerard
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Midorikawa, Gláucia Emy Okida
Pinheiro, Maria dos Reis Rodrigues
Santos, Bruna Vidigal dos
Arruda, Maricilia Conceição Cardoso de
Costa, Felipe F.
Pappas Júnior, Georgios Joannis
Ribeiro, Simone da Graça
Freire, Francisco
Miller, Robert Neil Gerard
dc.subject.por.fl_str_mv Aspergillus flavus
Detection
Internal amplification control
Mycotoxins
PCR
RAPD
rDNA ITS
topic Aspergillus flavus
Detection
Internal amplification control
Mycotoxins
PCR
RAPD
rDNA ITS
dc.description.abstract.por.fl_txt_mv Aims: The aim of this study was to determine the genetic variability in Aspergillus flavus populations from Brazil nut and cashew and develop a polymerase chain reaction (PCR) detection method. Methods and Results: Chomatography analysis of 48 isolates identified 36 as aflatoxigenic (75%). One hundred and forty-one DNA bands were generated with 11 random amplified polymorphic DNA (RAPD) primers and analysed via unweighted pair group analysis, using arithmetic means (UPGMA). Isolates grouped according to host, with differentiation of those from A. occidentale also according to geographical origin. Aspergillus flavus-specific PCR primers ASPITSF2 and ASPITSR3 were designed from ribosomal DNA internal transcribed spacers (ITS 1 and 2), and an internal amplification control was developed, to prevent false negative results. Specificity to only A. flavus was confirmed against DNA from additional aspergilli and other fungi. Conclusions: RAPD-based characterization differentiated isolates according to plant host. The PCR primer pair developed showed specificity to A. flavus, with a detection limit of 10 fg. Significance and Impact of the Study: Genetic variability observed in A. flavus isolates from two Brazilian agroecosystems suggested reproductive isolation. The PCR detection method developed for A. flavus represents progress towards multiplex PCR detection of aflatoxigenic and nonaflatoxigenic strains in Hazard Analysis Critical Control Point systems.
dc.description.status.pt_BR.fl_txt_mv Publicado
description Aims: The aim of this study was to determine the genetic variability in Aspergillus flavus populations from Brazil nut and cashew and develop a polymerase chain reaction (PCR) detection method. Methods and Results: Chomatography analysis of 48 isolates identified 36 as aflatoxigenic (75%). One hundred and forty-one DNA bands were generated with 11 random amplified polymorphic DNA (RAPD) primers and analysed via unweighted pair group analysis, using arithmetic means (UPGMA). Isolates grouped according to host, with differentiation of those from A. occidentale also according to geographical origin. Aspergillus flavus-specific PCR primers ASPITSF2 and ASPITSR3 were designed from ribosomal DNA internal transcribed spacers (ITS 1 and 2), and an internal amplification control was developed, to prevent false negative results. Specificity to only A. flavus was confirmed against DNA from additional aspergilli and other fungi. Conclusions: RAPD-based characterization differentiated isolates according to plant host. The PCR primer pair developed showed specificity to A. flavus, with a detection limit of 10 fg. Significance and Impact of the Study: Genetic variability observed in A. flavus isolates from two Brazilian agroecosystems suggested reproductive isolation. The PCR detection method developed for A. flavus represents progress towards multiplex PCR detection of aflatoxigenic and nonaflatoxigenic strains in Hazard Analysis Critical Control Point systems.
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2016-10-10T03:53:08Z
dc.date.available.fl_str_mv 2016-10-10T03:53:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
status_str publishedVersion
format article
dc.identifier.citation.fl_str_mv MIDORIKAWA, Gláucia Emy Okida et al. Characterization of Aspergillus flavus strains from Brazilian Brazil nut and cashew by RAPD and ribosomal DNA analysis. Letters in Applied Microbiology, v. 47, p. 12-18, 2008.
dc.identifier.uri.fl_str_mv http://twingo.ucb.br:8080/jspui/handle/10869/699
https://repositorio.ucb.br:9443/jspui/handle/123456789/7931
identifier_str_mv MIDORIKAWA, Gláucia Emy Okida et al. Characterization of Aspergillus flavus strains from Brazilian Brazil nut and cashew by RAPD and ribosomal DNA analysis. Letters in Applied Microbiology, v. 47, p. 12-18, 2008.
url http://twingo.ucb.br:8080/jspui/handle/10869/699
https://repositorio.ucb.br:9443/jspui/handle/123456789/7931
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reponame_str Repositório Institucional da UCB
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