Caracteriza????o e mapeamento de RGAS em Arachis

Detalhes bibliográficos
Autor(a) principal: Santos, Bruna Vidigal dos
Data de Publicação: 2009
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UCB
Texto Completo: https://bdtd.ucb.br:8443/jspui/handle/123456789/151
Resumo: Peanut (Arachis hypogaea) is one of the major oilseed crops worldwide. It is very susceptible to various biotic and abiotic stresses, to which many wild species are resistant. Introgression of useful genes from wild species in peanut can be accelerated by the use of molecular marker assisted selection (MAS), through the identification of markers closely linked to genes of interest such as resistances to diseases. QTLs for resistance to leaf-spot caused by the fungus C. personatum were mapped in the reference genetic map from the cross between A. duranensis and A. stenosperma. The RGA S1_A_36 that co-segregated with one QTL, located in Linkage Group 4, was used to identify positive clones in BAC libraries of two wild species of Arachis. The subcloning and sequencing of a full BAC containing the RGA enabled the characterization of this sequence, and its annotation using the gene prediction program RiceGAAS. In total 20 ORFs were found distributed in eight contigs. Of these, five ORFs contained resistance genes analogs of the TIR-NBSLRR family and four contained transposable elements, one DNA transposon and three retrotransposons of the LTR Ty1-Copia type. This work also enabled the mapping of five microsatellite markers, which were BAC derived, in the same linkage group of the RGA studied. Microsatellite markers are attractive as they are PCR based and show good reproducibility and transferability among related species. The identification of such markers associated with resistance loci is of great importance as it has the potential of speeding up the process of introgression of resistance genes in peanut.
id UCB_8e8f4cbeed9047d9e45d0fd84625f046
oai_identifier_str oai:bdtd.ucb.br:123456789/151
network_acronym_str UCB
network_name_str Biblioteca Digital de Teses e Dissertações da UCB
spelling Bertioli, David JohnCPF:72228822191http://genos.cnpq.br:12010/dwlattes/owa/prc_imp_cv_int?f_cod=K4761877H0Guimar??es, Patr??cia MessenbergMiller, Robert Neil GerardCPF:71747060100http://genos.cnpq.br:12010/dwlattes/owa/prc_imp_cv_int?f_cod=K4707614H4Buso, Glaucia Salles CortopassiCPF:22734201100http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4721466U3CPF:72228822191http://lattes.cnpq.br/0135817085530835Santos, Bruna Vidigal dos2016-06-24T04:01:40Z2009-08-272009-06-05SANTOS, Bruna Vidigal dos. Caracteriza????o e mapeamento de RGAS em Arachis. 2009. 138 f. Disserta????o (Mestrado em Ci??ncias Gen??micas, Gen??tica Molecular e de Popula????es, Biotecnologia Molecular) - Universidade Cat??lica de Bras??lia, Bras??lia, 2009.https://bdtd.ucb.br:8443/jspui/handle/123456789/151Peanut (Arachis hypogaea) is one of the major oilseed crops worldwide. It is very susceptible to various biotic and abiotic stresses, to which many wild species are resistant. Introgression of useful genes from wild species in peanut can be accelerated by the use of molecular marker assisted selection (MAS), through the identification of markers closely linked to genes of interest such as resistances to diseases. QTLs for resistance to leaf-spot caused by the fungus C. personatum were mapped in the reference genetic map from the cross between A. duranensis and A. stenosperma. The RGA S1_A_36 that co-segregated with one QTL, located in Linkage Group 4, was used to identify positive clones in BAC libraries of two wild species of Arachis. The subcloning and sequencing of a full BAC containing the RGA enabled the characterization of this sequence, and its annotation using the gene prediction program RiceGAAS. In total 20 ORFs were found distributed in eight contigs. Of these, five ORFs contained resistance genes analogs of the TIR-NBSLRR family and four contained transposable elements, one DNA transposon and three retrotransposons of the LTR Ty1-Copia type. This work also enabled the mapping of five microsatellite markers, which were BAC derived, in the same linkage group of the RGA studied. Microsatellite markers are attractive as they are PCR based and show good reproducibility and transferability among related species. The identification of such markers associated with resistance loci is of great importance as it has the potential of speeding up the process of introgression of resistance genes in peanut.O amendoim (Arachis hypogaea) est?? entre as principais oleaginosas cultivadas em todo o mundo. ?? bastante suscept??vel a diversos estresses bi??ticos e abi??ticos, para os quais muitas das esp??cies silvestres s??o resistentes. O processo de introgress??o de genes ??teis de esp??cies silvestres em amendoim pode ser acelerado pelo uso de sele????o assistida por marcadores moleculares, atrav??s da identifica????o de marcadores proximamente ligados a genes de interesse como os de resist??ncia a doen??as. QTLs (Quantitative Trait locus) para resist??ncia ?? mancha foliar causada pelo fungo C. personatum foram mapeados em mapa gen??tico de refer??ncia constru??do para o cruzamento entre A. duranensis e A. stenosperma. O RGA (Resistance Genes Analog) S1_A_36 que co-segrega com um destes QTLs, localizado no Grupo de Liga????o 4, foi utilizado para identificar clones positivos nas bibliotecas BAC (Bacterial Artificial Chromosome) de esp??cies silvestres do g??nero Arachis. A subclonagem e sequenciamento do BAC que cont??m este RGA possibilitou a caracteriza????o da sequ??ncia, identificando, com aux??lio do Programa de predi????o RiceGAAS, 20 ORFs distribu??das em oito contigs. Destas, cinco ORFs estavam relacionadas a genes de resist??ncia pertencentes ?? fam??lia TIR-NBS-LRR e quatro ORFs a elementos de transposi????o, sendo um transposon e tr??s retrotransposons LTR do tipo Ty1-Copia. Este trabalho possibilitou ainda, o mapeamento de cinco marcadores microssat??lites derivados do BAC, que mapearam no mesmo grupo de liga????o dos (RGAs). A utiliza????o de marcadores microssat??lites exibe vantagens adicionais ?? utiliza????o de RGAs no processo de sele????o assistida, como a praticidade operacional, alta reprodutibilidade, por ser baseado em PCR, e principalmente por ser um marcador transfer??vel entre esp??cies relacionadas. A identifica????o de marcadores mais robustos e transfer??veis associados a locos que conferem resist??ncia possibilitar?? a sele????o assistida nos programas de melhoramento gen??tico, o que acelerar?? o processo de introgress??o dos genes de resist??ncia no amendoim.Made available in DSpace on 2016-06-24T04:01:40Z (GMT). No. of bitstreams: 1 Texto completo Bruna Vidigal - 2009.pdf: 2885284 bytes, checksum: 1006b2c988b343229344f7f1ac507bd7 (MD5) Previous issue date: 2009-06-05application/pdfhttps://bdtd.ucb.br:8443/jspui/retrieve/2087/Texto%20completo%20Bruna%20Vidigal%20-%202009.pdf.jpgporUniversidade Cat??lica de Bras??liaPrograma de P??s-Gradua????o em Ci??ncias Gen??micas e BiotecnologiaUCBBRCi??ncias Gen??micas, Gen??tica Molecular e de Popula????es, Biotecnologia MolecularGen??tica vegetalCNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL::SILVICULTURA::GENETICA E MELHORAMENTO FLORESTALCaracteriza????o e mapeamento de RGAS em ArachisCaracteriza????o e mapeamento de RGAS em Arachisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UCBinstname:Universidade Católica de Brasília (UCB)instacron:UCBORIGINALTexto completo Bruna Vidigal - 2009.pdfapplication/pdf2885284https://200.214.135.178:8443/jspui/bitstream/123456789/151/1/Texto+completo+Bruna+Vidigal+-+2009.pdf1006b2c988b343229344f7f1ac507bd7MD51THUMBNAILTexto completo Bruna Vidigal - 2009.pdf.jpgTexto completo Bruna Vidigal - 2009.pdf.jpgimage/jpeg7904https://200.214.135.178:8443/jspui/bitstream/123456789/151/2/Texto+completo+Bruna+Vidigal+-+2009.pdf.jpg3b4035d160c135565e659bb62d59ca2cMD52123456789/1512020-06-22 20:53:00.961Biblioteca Digital de Teses e Dissertaçõeshttps://bdtd.ucb.br:8443/jspui/
dc.title.por.fl_str_mv Caracteriza????o e mapeamento de RGAS em Arachis
Caracteriza????o e mapeamento de RGAS em Arachis
title Caracteriza????o e mapeamento de RGAS em Arachis
spellingShingle Caracteriza????o e mapeamento de RGAS em Arachis
Santos, Bruna Vidigal dos
Gen??tica vegetal
CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL::SILVICULTURA::GENETICA E MELHORAMENTO FLORESTAL
title_short Caracteriza????o e mapeamento de RGAS em Arachis
title_full Caracteriza????o e mapeamento de RGAS em Arachis
title_fullStr Caracteriza????o e mapeamento de RGAS em Arachis
title_full_unstemmed Caracteriza????o e mapeamento de RGAS em Arachis
title_sort Caracteriza????o e mapeamento de RGAS em Arachis
author Santos, Bruna Vidigal dos
author_facet Santos, Bruna Vidigal dos
author_role author
dc.contributor.advisor1.fl_str_mv Bertioli, David John
dc.contributor.advisor1ID.fl_str_mv CPF:72228822191
dc.contributor.advisor1Lattes.fl_str_mv http://genos.cnpq.br:12010/dwlattes/owa/prc_imp_cv_int?f_cod=K4761877H0
dc.contributor.advisor-co1.fl_str_mv Guimar??es, Patr??cia Messenberg
dc.contributor.referee1.fl_str_mv Miller, Robert Neil Gerard
dc.contributor.referee1ID.fl_str_mv CPF:71747060100
dc.contributor.referee1Lattes.fl_str_mv http://genos.cnpq.br:12010/dwlattes/owa/prc_imp_cv_int?f_cod=K4707614H4
dc.contributor.referee2.fl_str_mv Buso, Glaucia Salles Cortopassi
dc.contributor.referee2ID.fl_str_mv CPF:22734201100
dc.contributor.referee2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4721466U3
dc.contributor.authorID.fl_str_mv CPF:72228822191
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/0135817085530835
dc.contributor.author.fl_str_mv Santos, Bruna Vidigal dos
contributor_str_mv Bertioli, David John
Guimar??es, Patr??cia Messenberg
Miller, Robert Neil Gerard
Buso, Glaucia Salles Cortopassi
dc.subject.por.fl_str_mv Gen??tica vegetal
topic Gen??tica vegetal
CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL::SILVICULTURA::GENETICA E MELHORAMENTO FLORESTAL
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL::SILVICULTURA::GENETICA E MELHORAMENTO FLORESTAL
dc.description.abstract.eng.fl_txt_mv Peanut (Arachis hypogaea) is one of the major oilseed crops worldwide. It is very susceptible to various biotic and abiotic stresses, to which many wild species are resistant. Introgression of useful genes from wild species in peanut can be accelerated by the use of molecular marker assisted selection (MAS), through the identification of markers closely linked to genes of interest such as resistances to diseases. QTLs for resistance to leaf-spot caused by the fungus C. personatum were mapped in the reference genetic map from the cross between A. duranensis and A. stenosperma. The RGA S1_A_36 that co-segregated with one QTL, located in Linkage Group 4, was used to identify positive clones in BAC libraries of two wild species of Arachis. The subcloning and sequencing of a full BAC containing the RGA enabled the characterization of this sequence, and its annotation using the gene prediction program RiceGAAS. In total 20 ORFs were found distributed in eight contigs. Of these, five ORFs contained resistance genes analogs of the TIR-NBSLRR family and four contained transposable elements, one DNA transposon and three retrotransposons of the LTR Ty1-Copia type. This work also enabled the mapping of five microsatellite markers, which were BAC derived, in the same linkage group of the RGA studied. Microsatellite markers are attractive as they are PCR based and show good reproducibility and transferability among related species. The identification of such markers associated with resistance loci is of great importance as it has the potential of speeding up the process of introgression of resistance genes in peanut.
dc.description.abstract.por.fl_txt_mv O amendoim (Arachis hypogaea) est?? entre as principais oleaginosas cultivadas em todo o mundo. ?? bastante suscept??vel a diversos estresses bi??ticos e abi??ticos, para os quais muitas das esp??cies silvestres s??o resistentes. O processo de introgress??o de genes ??teis de esp??cies silvestres em amendoim pode ser acelerado pelo uso de sele????o assistida por marcadores moleculares, atrav??s da identifica????o de marcadores proximamente ligados a genes de interesse como os de resist??ncia a doen??as. QTLs (Quantitative Trait locus) para resist??ncia ?? mancha foliar causada pelo fungo C. personatum foram mapeados em mapa gen??tico de refer??ncia constru??do para o cruzamento entre A. duranensis e A. stenosperma. O RGA (Resistance Genes Analog) S1_A_36 que co-segrega com um destes QTLs, localizado no Grupo de Liga????o 4, foi utilizado para identificar clones positivos nas bibliotecas BAC (Bacterial Artificial Chromosome) de esp??cies silvestres do g??nero Arachis. A subclonagem e sequenciamento do BAC que cont??m este RGA possibilitou a caracteriza????o da sequ??ncia, identificando, com aux??lio do Programa de predi????o RiceGAAS, 20 ORFs distribu??das em oito contigs. Destas, cinco ORFs estavam relacionadas a genes de resist??ncia pertencentes ?? fam??lia TIR-NBS-LRR e quatro ORFs a elementos de transposi????o, sendo um transposon e tr??s retrotransposons LTR do tipo Ty1-Copia. Este trabalho possibilitou ainda, o mapeamento de cinco marcadores microssat??lites derivados do BAC, que mapearam no mesmo grupo de liga????o dos (RGAs). A utiliza????o de marcadores microssat??lites exibe vantagens adicionais ?? utiliza????o de RGAs no processo de sele????o assistida, como a praticidade operacional, alta reprodutibilidade, por ser baseado em PCR, e principalmente por ser um marcador transfer??vel entre esp??cies relacionadas. A identifica????o de marcadores mais robustos e transfer??veis associados a locos que conferem resist??ncia possibilitar?? a sele????o assistida nos programas de melhoramento gen??tico, o que acelerar?? o processo de introgress??o dos genes de resist??ncia no amendoim.
description Peanut (Arachis hypogaea) is one of the major oilseed crops worldwide. It is very susceptible to various biotic and abiotic stresses, to which many wild species are resistant. Introgression of useful genes from wild species in peanut can be accelerated by the use of molecular marker assisted selection (MAS), through the identification of markers closely linked to genes of interest such as resistances to diseases. QTLs for resistance to leaf-spot caused by the fungus C. personatum were mapped in the reference genetic map from the cross between A. duranensis and A. stenosperma. The RGA S1_A_36 that co-segregated with one QTL, located in Linkage Group 4, was used to identify positive clones in BAC libraries of two wild species of Arachis. The subcloning and sequencing of a full BAC containing the RGA enabled the characterization of this sequence, and its annotation using the gene prediction program RiceGAAS. In total 20 ORFs were found distributed in eight contigs. Of these, five ORFs contained resistance genes analogs of the TIR-NBSLRR family and four contained transposable elements, one DNA transposon and three retrotransposons of the LTR Ty1-Copia type. This work also enabled the mapping of five microsatellite markers, which were BAC derived, in the same linkage group of the RGA studied. Microsatellite markers are attractive as they are PCR based and show good reproducibility and transferability among related species. The identification of such markers associated with resistance loci is of great importance as it has the potential of speeding up the process of introgression of resistance genes in peanut.
publishDate 2009
dc.date.available.fl_str_mv 2009-08-27
dc.date.issued.fl_str_mv 2009-06-05
dc.date.accessioned.fl_str_mv 2016-06-24T04:01:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
status_str publishedVersion
format masterThesis
dc.identifier.citation.fl_str_mv SANTOS, Bruna Vidigal dos. Caracteriza????o e mapeamento de RGAS em Arachis. 2009. 138 f. Disserta????o (Mestrado em Ci??ncias Gen??micas, Gen??tica Molecular e de Popula????es, Biotecnologia Molecular) - Universidade Cat??lica de Bras??lia, Bras??lia, 2009.
dc.identifier.uri.fl_str_mv https://bdtd.ucb.br:8443/jspui/handle/123456789/151
identifier_str_mv SANTOS, Bruna Vidigal dos. Caracteriza????o e mapeamento de RGAS em Arachis. 2009. 138 f. Disserta????o (Mestrado em Ci??ncias Gen??micas, Gen??tica Molecular e de Popula????es, Biotecnologia Molecular) - Universidade Cat??lica de Bras??lia, Bras??lia, 2009.
url https://bdtd.ucb.br:8443/jspui/handle/123456789/151
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Cat??lica de Bras??lia
dc.publisher.program.fl_str_mv Programa de P??s-Gradua????o em Ci??ncias Gen??micas e Biotecnologia
dc.publisher.initials.fl_str_mv UCB
dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Ci??ncias Gen??micas, Gen??tica Molecular e de Popula????es, Biotecnologia Molecular
publisher.none.fl_str_mv Universidade Cat??lica de Bras??lia
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UCB
instname:Universidade Católica de Brasília (UCB)
instacron:UCB
instname_str Universidade Católica de Brasília (UCB)
instacron_str UCB
institution UCB
reponame_str Biblioteca Digital de Teses e Dissertações da UCB
collection Biblioteca Digital de Teses e Dissertações da UCB
bitstream.url.fl_str_mv https://200.214.135.178:8443/jspui/bitstream/123456789/151/1/Texto+completo+Bruna+Vidigal+-+2009.pdf
https://200.214.135.178:8443/jspui/bitstream/123456789/151/2/Texto+completo+Bruna+Vidigal+-+2009.pdf.jpg
bitstream.checksum.fl_str_mv 1006b2c988b343229344f7f1ac507bd7
3b4035d160c135565e659bb62d59ca2c
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1724829771361157120