Caracteriza????o e mapeamento de RGAS em Arachis
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UCB |
Texto Completo: | https://bdtd.ucb.br:8443/jspui/handle/123456789/151 |
Resumo: | Peanut (Arachis hypogaea) is one of the major oilseed crops worldwide. It is very susceptible to various biotic and abiotic stresses, to which many wild species are resistant. Introgression of useful genes from wild species in peanut can be accelerated by the use of molecular marker assisted selection (MAS), through the identification of markers closely linked to genes of interest such as resistances to diseases. QTLs for resistance to leaf-spot caused by the fungus C. personatum were mapped in the reference genetic map from the cross between A. duranensis and A. stenosperma. The RGA S1_A_36 that co-segregated with one QTL, located in Linkage Group 4, was used to identify positive clones in BAC libraries of two wild species of Arachis. The subcloning and sequencing of a full BAC containing the RGA enabled the characterization of this sequence, and its annotation using the gene prediction program RiceGAAS. In total 20 ORFs were found distributed in eight contigs. Of these, five ORFs contained resistance genes analogs of the TIR-NBSLRR family and four contained transposable elements, one DNA transposon and three retrotransposons of the LTR Ty1-Copia type. This work also enabled the mapping of five microsatellite markers, which were BAC derived, in the same linkage group of the RGA studied. Microsatellite markers are attractive as they are PCR based and show good reproducibility and transferability among related species. The identification of such markers associated with resistance loci is of great importance as it has the potential of speeding up the process of introgression of resistance genes in peanut. |
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Bertioli, David JohnCPF:72228822191http://genos.cnpq.br:12010/dwlattes/owa/prc_imp_cv_int?f_cod=K4761877H0Guimar??es, Patr??cia MessenbergMiller, Robert Neil GerardCPF:71747060100http://genos.cnpq.br:12010/dwlattes/owa/prc_imp_cv_int?f_cod=K4707614H4Buso, Glaucia Salles CortopassiCPF:22734201100http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4721466U3CPF:72228822191http://lattes.cnpq.br/0135817085530835Santos, Bruna Vidigal dos2016-06-24T04:01:40Z2009-08-272009-06-05SANTOS, Bruna Vidigal dos. Caracteriza????o e mapeamento de RGAS em Arachis. 2009. 138 f. Disserta????o (Mestrado em Ci??ncias Gen??micas, Gen??tica Molecular e de Popula????es, Biotecnologia Molecular) - Universidade Cat??lica de Bras??lia, Bras??lia, 2009.https://bdtd.ucb.br:8443/jspui/handle/123456789/151Peanut (Arachis hypogaea) is one of the major oilseed crops worldwide. It is very susceptible to various biotic and abiotic stresses, to which many wild species are resistant. Introgression of useful genes from wild species in peanut can be accelerated by the use of molecular marker assisted selection (MAS), through the identification of markers closely linked to genes of interest such as resistances to diseases. QTLs for resistance to leaf-spot caused by the fungus C. personatum were mapped in the reference genetic map from the cross between A. duranensis and A. stenosperma. The RGA S1_A_36 that co-segregated with one QTL, located in Linkage Group 4, was used to identify positive clones in BAC libraries of two wild species of Arachis. The subcloning and sequencing of a full BAC containing the RGA enabled the characterization of this sequence, and its annotation using the gene prediction program RiceGAAS. In total 20 ORFs were found distributed in eight contigs. Of these, five ORFs contained resistance genes analogs of the TIR-NBSLRR family and four contained transposable elements, one DNA transposon and three retrotransposons of the LTR Ty1-Copia type. This work also enabled the mapping of five microsatellite markers, which were BAC derived, in the same linkage group of the RGA studied. Microsatellite markers are attractive as they are PCR based and show good reproducibility and transferability among related species. The identification of such markers associated with resistance loci is of great importance as it has the potential of speeding up the process of introgression of resistance genes in peanut.O amendoim (Arachis hypogaea) est?? entre as principais oleaginosas cultivadas em todo o mundo. ?? bastante suscept??vel a diversos estresses bi??ticos e abi??ticos, para os quais muitas das esp??cies silvestres s??o resistentes. O processo de introgress??o de genes ??teis de esp??cies silvestres em amendoim pode ser acelerado pelo uso de sele????o assistida por marcadores moleculares, atrav??s da identifica????o de marcadores proximamente ligados a genes de interesse como os de resist??ncia a doen??as. QTLs (Quantitative Trait locus) para resist??ncia ?? mancha foliar causada pelo fungo C. personatum foram mapeados em mapa gen??tico de refer??ncia constru??do para o cruzamento entre A. duranensis e A. stenosperma. O RGA (Resistance Genes Analog) S1_A_36 que co-segrega com um destes QTLs, localizado no Grupo de Liga????o 4, foi utilizado para identificar clones positivos nas bibliotecas BAC (Bacterial Artificial Chromosome) de esp??cies silvestres do g??nero Arachis. A subclonagem e sequenciamento do BAC que cont??m este RGA possibilitou a caracteriza????o da sequ??ncia, identificando, com aux??lio do Programa de predi????o RiceGAAS, 20 ORFs distribu??das em oito contigs. Destas, cinco ORFs estavam relacionadas a genes de resist??ncia pertencentes ?? fam??lia TIR-NBS-LRR e quatro ORFs a elementos de transposi????o, sendo um transposon e tr??s retrotransposons LTR do tipo Ty1-Copia. Este trabalho possibilitou ainda, o mapeamento de cinco marcadores microssat??lites derivados do BAC, que mapearam no mesmo grupo de liga????o dos (RGAs). A utiliza????o de marcadores microssat??lites exibe vantagens adicionais ?? utiliza????o de RGAs no processo de sele????o assistida, como a praticidade operacional, alta reprodutibilidade, por ser baseado em PCR, e principalmente por ser um marcador transfer??vel entre esp??cies relacionadas. A identifica????o de marcadores mais robustos e transfer??veis associados a locos que conferem resist??ncia possibilitar?? a sele????o assistida nos programas de melhoramento gen??tico, o que acelerar?? o processo de introgress??o dos genes de resist??ncia no amendoim.Made available in DSpace on 2016-06-24T04:01:40Z (GMT). No. of bitstreams: 1 Texto completo Bruna Vidigal - 2009.pdf: 2885284 bytes, checksum: 1006b2c988b343229344f7f1ac507bd7 (MD5) Previous issue date: 2009-06-05application/pdfhttps://bdtd.ucb.br:8443/jspui/retrieve/2087/Texto%20completo%20Bruna%20Vidigal%20-%202009.pdf.jpgporUniversidade Cat??lica de Bras??liaPrograma de P??s-Gradua????o em Ci??ncias Gen??micas e BiotecnologiaUCBBRCi??ncias Gen??micas, Gen??tica Molecular e de Popula????es, Biotecnologia MolecularGen??tica vegetalCNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL::SILVICULTURA::GENETICA E MELHORAMENTO FLORESTALCaracteriza????o e mapeamento de RGAS em ArachisCaracteriza????o e mapeamento de RGAS em Arachisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UCBinstname:Universidade Católica de Brasília (UCB)instacron:UCBORIGINALTexto completo Bruna Vidigal - 2009.pdfapplication/pdf2885284https://200.214.135.178:8443/jspui/bitstream/123456789/151/1/Texto+completo+Bruna+Vidigal+-+2009.pdf1006b2c988b343229344f7f1ac507bd7MD51THUMBNAILTexto completo Bruna Vidigal - 2009.pdf.jpgTexto completo Bruna Vidigal - 2009.pdf.jpgimage/jpeg7904https://200.214.135.178:8443/jspui/bitstream/123456789/151/2/Texto+completo+Bruna+Vidigal+-+2009.pdf.jpg3b4035d160c135565e659bb62d59ca2cMD52123456789/1512020-06-22 20:53:00.961Biblioteca Digital de Teses e Dissertaçõeshttps://bdtd.ucb.br:8443/jspui/ |
dc.title.por.fl_str_mv |
Caracteriza????o e mapeamento de RGAS em Arachis Caracteriza????o e mapeamento de RGAS em Arachis |
title |
Caracteriza????o e mapeamento de RGAS em Arachis |
spellingShingle |
Caracteriza????o e mapeamento de RGAS em Arachis Santos, Bruna Vidigal dos Gen??tica vegetal CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL::SILVICULTURA::GENETICA E MELHORAMENTO FLORESTAL |
title_short |
Caracteriza????o e mapeamento de RGAS em Arachis |
title_full |
Caracteriza????o e mapeamento de RGAS em Arachis |
title_fullStr |
Caracteriza????o e mapeamento de RGAS em Arachis |
title_full_unstemmed |
Caracteriza????o e mapeamento de RGAS em Arachis |
title_sort |
Caracteriza????o e mapeamento de RGAS em Arachis |
author |
Santos, Bruna Vidigal dos |
author_facet |
Santos, Bruna Vidigal dos |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Bertioli, David John |
dc.contributor.advisor1ID.fl_str_mv |
CPF:72228822191 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://genos.cnpq.br:12010/dwlattes/owa/prc_imp_cv_int?f_cod=K4761877H0 |
dc.contributor.advisor-co1.fl_str_mv |
Guimar??es, Patr??cia Messenberg |
dc.contributor.referee1.fl_str_mv |
Miller, Robert Neil Gerard |
dc.contributor.referee1ID.fl_str_mv |
CPF:71747060100 |
dc.contributor.referee1Lattes.fl_str_mv |
http://genos.cnpq.br:12010/dwlattes/owa/prc_imp_cv_int?f_cod=K4707614H4 |
dc.contributor.referee2.fl_str_mv |
Buso, Glaucia Salles Cortopassi |
dc.contributor.referee2ID.fl_str_mv |
CPF:22734201100 |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4721466U3 |
dc.contributor.authorID.fl_str_mv |
CPF:72228822191 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0135817085530835 |
dc.contributor.author.fl_str_mv |
Santos, Bruna Vidigal dos |
contributor_str_mv |
Bertioli, David John Guimar??es, Patr??cia Messenberg Miller, Robert Neil Gerard Buso, Glaucia Salles Cortopassi |
dc.subject.por.fl_str_mv |
Gen??tica vegetal |
topic |
Gen??tica vegetal CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL::SILVICULTURA::GENETICA E MELHORAMENTO FLORESTAL |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL::SILVICULTURA::GENETICA E MELHORAMENTO FLORESTAL |
dc.description.abstract.eng.fl_txt_mv |
Peanut (Arachis hypogaea) is one of the major oilseed crops worldwide. It is very susceptible to various biotic and abiotic stresses, to which many wild species are resistant. Introgression of useful genes from wild species in peanut can be accelerated by the use of molecular marker assisted selection (MAS), through the identification of markers closely linked to genes of interest such as resistances to diseases. QTLs for resistance to leaf-spot caused by the fungus C. personatum were mapped in the reference genetic map from the cross between A. duranensis and A. stenosperma. The RGA S1_A_36 that co-segregated with one QTL, located in Linkage Group 4, was used to identify positive clones in BAC libraries of two wild species of Arachis. The subcloning and sequencing of a full BAC containing the RGA enabled the characterization of this sequence, and its annotation using the gene prediction program RiceGAAS. In total 20 ORFs were found distributed in eight contigs. Of these, five ORFs contained resistance genes analogs of the TIR-NBSLRR family and four contained transposable elements, one DNA transposon and three retrotransposons of the LTR Ty1-Copia type. This work also enabled the mapping of five microsatellite markers, which were BAC derived, in the same linkage group of the RGA studied. Microsatellite markers are attractive as they are PCR based and show good reproducibility and transferability among related species. The identification of such markers associated with resistance loci is of great importance as it has the potential of speeding up the process of introgression of resistance genes in peanut. |
dc.description.abstract.por.fl_txt_mv |
O amendoim (Arachis hypogaea) est?? entre as principais oleaginosas cultivadas em todo o mundo. ?? bastante suscept??vel a diversos estresses bi??ticos e abi??ticos, para os quais muitas das esp??cies silvestres s??o resistentes. O processo de introgress??o de genes ??teis de esp??cies silvestres em amendoim pode ser acelerado pelo uso de sele????o assistida por marcadores moleculares, atrav??s da identifica????o de marcadores proximamente ligados a genes de interesse como os de resist??ncia a doen??as. QTLs (Quantitative Trait locus) para resist??ncia ?? mancha foliar causada pelo fungo C. personatum foram mapeados em mapa gen??tico de refer??ncia constru??do para o cruzamento entre A. duranensis e A. stenosperma. O RGA (Resistance Genes Analog) S1_A_36 que co-segrega com um destes QTLs, localizado no Grupo de Liga????o 4, foi utilizado para identificar clones positivos nas bibliotecas BAC (Bacterial Artificial Chromosome) de esp??cies silvestres do g??nero Arachis. A subclonagem e sequenciamento do BAC que cont??m este RGA possibilitou a caracteriza????o da sequ??ncia, identificando, com aux??lio do Programa de predi????o RiceGAAS, 20 ORFs distribu??das em oito contigs. Destas, cinco ORFs estavam relacionadas a genes de resist??ncia pertencentes ?? fam??lia TIR-NBS-LRR e quatro ORFs a elementos de transposi????o, sendo um transposon e tr??s retrotransposons LTR do tipo Ty1-Copia. Este trabalho possibilitou ainda, o mapeamento de cinco marcadores microssat??lites derivados do BAC, que mapearam no mesmo grupo de liga????o dos (RGAs). A utiliza????o de marcadores microssat??lites exibe vantagens adicionais ?? utiliza????o de RGAs no processo de sele????o assistida, como a praticidade operacional, alta reprodutibilidade, por ser baseado em PCR, e principalmente por ser um marcador transfer??vel entre esp??cies relacionadas. A identifica????o de marcadores mais robustos e transfer??veis associados a locos que conferem resist??ncia possibilitar?? a sele????o assistida nos programas de melhoramento gen??tico, o que acelerar?? o processo de introgress??o dos genes de resist??ncia no amendoim. |
description |
Peanut (Arachis hypogaea) is one of the major oilseed crops worldwide. It is very susceptible to various biotic and abiotic stresses, to which many wild species are resistant. Introgression of useful genes from wild species in peanut can be accelerated by the use of molecular marker assisted selection (MAS), through the identification of markers closely linked to genes of interest such as resistances to diseases. QTLs for resistance to leaf-spot caused by the fungus C. personatum were mapped in the reference genetic map from the cross between A. duranensis and A. stenosperma. The RGA S1_A_36 that co-segregated with one QTL, located in Linkage Group 4, was used to identify positive clones in BAC libraries of two wild species of Arachis. The subcloning and sequencing of a full BAC containing the RGA enabled the characterization of this sequence, and its annotation using the gene prediction program RiceGAAS. In total 20 ORFs were found distributed in eight contigs. Of these, five ORFs contained resistance genes analogs of the TIR-NBSLRR family and four contained transposable elements, one DNA transposon and three retrotransposons of the LTR Ty1-Copia type. This work also enabled the mapping of five microsatellite markers, which were BAC derived, in the same linkage group of the RGA studied. Microsatellite markers are attractive as they are PCR based and show good reproducibility and transferability among related species. The identification of such markers associated with resistance loci is of great importance as it has the potential of speeding up the process of introgression of resistance genes in peanut. |
publishDate |
2009 |
dc.date.available.fl_str_mv |
2009-08-27 |
dc.date.issued.fl_str_mv |
2009-06-05 |
dc.date.accessioned.fl_str_mv |
2016-06-24T04:01:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
status_str |
publishedVersion |
format |
masterThesis |
dc.identifier.citation.fl_str_mv |
SANTOS, Bruna Vidigal dos. Caracteriza????o e mapeamento de RGAS em Arachis. 2009. 138 f. Disserta????o (Mestrado em Ci??ncias Gen??micas, Gen??tica Molecular e de Popula????es, Biotecnologia Molecular) - Universidade Cat??lica de Bras??lia, Bras??lia, 2009. |
dc.identifier.uri.fl_str_mv |
https://bdtd.ucb.br:8443/jspui/handle/123456789/151 |
identifier_str_mv |
SANTOS, Bruna Vidigal dos. Caracteriza????o e mapeamento de RGAS em Arachis. 2009. 138 f. Disserta????o (Mestrado em Ci??ncias Gen??micas, Gen??tica Molecular e de Popula????es, Biotecnologia Molecular) - Universidade Cat??lica de Bras??lia, Bras??lia, 2009. |
url |
https://bdtd.ucb.br:8443/jspui/handle/123456789/151 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Cat??lica de Bras??lia |
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Programa de P??s-Gradua????o em Ci??ncias Gen??micas e Biotecnologia |
dc.publisher.initials.fl_str_mv |
UCB |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Ci??ncias Gen??micas, Gen??tica Molecular e de Popula????es, Biotecnologia Molecular |
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Universidade Cat??lica de Bras??lia |
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