Avalia????o do potencial de fra????es do soro humano em substitui????o ao soro fetal bovino no cultivo de c??lulas-tronco mesenquimais humanas

• Autor(a) principal: Paiva, Bianca Simonassi Raso de
• Data de Publicação: 2019
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UCB
• Texto Completo: https://bdtd.ucb.br:8443/jspui/handle/tede/2556
• topic: Senesc??ncia; Soro humano; C??lulas-tronco mesenquimais; Mesenchymal stem cell; Human serum; Senescence; CNPQ::CIENCIAS BIOLOGICAS::GENETICA

Mesenchymal stem cells (MSCs) have immunomodulation potential and ability to differentiate into multi-lineages, which makes them a tool of interest in cell therapy. Toxicological, immunological and contamination risks are concerns in the culture of cells for cell therapy, and may be associated with the use of animal supplements, such as fetal bovine serum (FBS). Human cell cultures have been successful in supplementing human serum in substitution for FBS, which may address problems eventually attributed to the use of non-species-specific supplements. In parallel, studies with extracellular vesicles (EVs) have demonstrated great potential of these particles in cell culture, being able, for example, to induce the differentiation of stem cells. This project aims to evaluate the potential of extracellular vesicles derived from pooled adult human serum (HS) regarding the ability to support the culture of human MSCs derived from adipose tissue (n = 3). FBS, HS, and extracellular vesicles-depleted adult human serum (DS) were also evaluated. The groups were evaluated for proliferation, viability, senescence and cell differentiation. Extracellular vesicles from human serum were not able to maintain the proliferation and viability potential of MSCs compared to the other groups. Among FBS, HS and DS groups, cells cultured with human serum and depleted human serum had higher proliferative potential in the first passages, and those cultured with FBS at later passages, but cell viability at all passages was higher in cells cultured with FBS. In relation to cell senescence, there was no difference between cells cultured with FBS and HS, but culture with DS resulted in a lower percentage of senescent cells. All sera maintained chondrogenic, osteogenic and adipogenic potential of MSCs. In addition, differences in the response of different MSCs isolates were observed in relation to serum types, indicating that the origin of the cells influenced their behavior and suggesting the need for studies with a higher number of isolates. Finally, while other studies have shown that complete human serum is an appropriate substitute for FBS, this study showed that DS is also a possible substitute for fetal bovine serum, with potential use in culturing MSCs to isolate EVs produced by these cells. In particular, depleted human serum stood out for having fewer senescent cells than the other groups.