BIOPROSPECÇÃO DE FUNGOS PRODUTORES DE PROTEASES: POTENCIAL PROTEOLÍTICO DE Fusarium oxysporum

Detalhes bibliográficos
Autor(a) principal: Prolo, Thaiane
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações do UNICENTRO
Texto Completo: http://localhost:8080/tede/handle/tede/393
Resumo: The proteolytic enzymes are one of the most important groups of commercial enzymes, representing about 60% of the global market for enzymes. Among the biotechnological applications of proteases, can be mentioned their application in detergents, leather, food, and other industries. The objective of this study was selection fungal that was better protease producers. To this, 73 fungal species were evaluated, 35 yeasts and 38 filamentous fungi. After an initial selection, the effect of various physico-chemical parameters on proteases production by the strain previously selected and subsequently identified as Fusarium oxysporum was investigated, using dog hair in white and black colors as substrates. The experiments were performed using Central composite rotational design applicable to Response Surface Methodology. Proteases produced were biochemically characterized, determining the following parameters: optimum temperature, optimum pH, thermostability, stability at different pH and the influence of ions and substances. Among the evaluated fungal strains, F. oxysporum produced the highest levels of alkaline protease when its was grown in black dog hair. Maximum production levels were achieved when the pH of medium was adjusted to 4.2, substrate concentration was 4.2% and the cultivation time was 8.6 days, corresponding to 72.8 U mL?¹. When it was grown in white dog hair, maximum production levels were obtained in pH of medium adjusted to 6.0, on substrate concentration of 5.0% and cultivation time of 6.5 days, corresponding to 51.84 U mL?¹. Productivity levels obtained employing white and black dog hair as substrates correspond an increase of 14.85 and 7.19 fold, respectively, in relation to the initial activity observed. Both enzymes exhibited desirable properties to be apply in many industries, such as relative thermal stability and action in a wide pH range. Thus, dog hair is a promising residue for alkaline proteases production, in both colors that were tested, due to be a low cost material and generated in large amounts by pet shops throughout the world.
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spelling Knob, AdrianaCPF:00101001001http://lattes.cnpq.br/1289646338155108Lima, Vanderlei Aparecido deCPF:00000000104http://lattes.cnpq.br/9090461949264421CPF:94658412268http://lattes.cnpq.br/7686288883788281Prolo, Thaiane2016-09-20T12:29:25Z2015-11-232015-02-13PROLO, Thaiane. não consta. 2015. 101 f. Dissertação (Mestrado em Biologia Evolutiva) - UNICENTRO - Universidade Estadual do Centro Oeste, Guarapuava, 2015.http://localhost:8080/tede/handle/tede/393The proteolytic enzymes are one of the most important groups of commercial enzymes, representing about 60% of the global market for enzymes. Among the biotechnological applications of proteases, can be mentioned their application in detergents, leather, food, and other industries. The objective of this study was selection fungal that was better protease producers. To this, 73 fungal species were evaluated, 35 yeasts and 38 filamentous fungi. After an initial selection, the effect of various physico-chemical parameters on proteases production by the strain previously selected and subsequently identified as Fusarium oxysporum was investigated, using dog hair in white and black colors as substrates. The experiments were performed using Central composite rotational design applicable to Response Surface Methodology. Proteases produced were biochemically characterized, determining the following parameters: optimum temperature, optimum pH, thermostability, stability at different pH and the influence of ions and substances. Among the evaluated fungal strains, F. oxysporum produced the highest levels of alkaline protease when its was grown in black dog hair. Maximum production levels were achieved when the pH of medium was adjusted to 4.2, substrate concentration was 4.2% and the cultivation time was 8.6 days, corresponding to 72.8 U mL?¹. When it was grown in white dog hair, maximum production levels were obtained in pH of medium adjusted to 6.0, on substrate concentration of 5.0% and cultivation time of 6.5 days, corresponding to 51.84 U mL?¹. Productivity levels obtained employing white and black dog hair as substrates correspond an increase of 14.85 and 7.19 fold, respectively, in relation to the initial activity observed. Both enzymes exhibited desirable properties to be apply in many industries, such as relative thermal stability and action in a wide pH range. Thus, dog hair is a promising residue for alkaline proteases production, in both colors that were tested, due to be a low cost material and generated in large amounts by pet shops throughout the world.As proteases constituem um dos mais importantes grupos enzimáticos comerciais, representando cerca de 60% do mercado mundial de enzimas. Dentre suas principais aplicações biotecnológicas, podem ser citadas suas utilizações nas indústrias de detergentes, couro, alimentos, entre outros. O presente estudo teve como objetivo selecionar fungos produtores de proteases. Para tal, 73 espécies fúngicas foram avaliadas, sendo 35 leveduras e 38 fungos filamentosos. Após uma seleção inicial, o efeito de diversos parâmetros físico- químicos sobre a produção de proteases pela linhagem previamente selecionada e posteriormente identificada como Fusarium oxysporum foi investigado, empregando-se pelo de cachorro nas colorações branca e preta como substratos. Os experimentos foram realizados em Delineamento Central Composto Rotacional aplicável à Metodologia de Superfície de Resposta. As proteases produzidas foram caracterizadas bioquimicamente, sendo determinados os seguintes parâmetros: temperatura ótima, pH ótimo, termoestabilidade, estabilidade em diferentes pH e a influência de íons e outras substâncias. Dentre as linhagens fúngicas avaliadas, F. oxysporum foi a que produziu os maiores níveis de proteases alcalinas, quando cultivada em pelo de cachorro na coloração preta. Níveis máximos de produção foram alcançados quando o pH do meio foi ajustado para 4,2, concentração do substrato de 4,2 % e o tempo de cultivo de 8,6 dias, correspondendo a 72,8 U mL?¹. Quando cultivado em pelo de cachorro na coloração branca, os níveis máximos de produção foram obtidos em pH do meio ajustado para 6,0, concentração de substrato 5,0 % e tempo de cultivo de 6,5 dias, correspondendo a 51,84 U mL?¹. Os níveis de produtividade obtidos empregando-se o pelo branco e o pelo preto como substratos corresponderam a um aumento de 14,85 e 7,19 vezes, respectivamente, em relação a atividade inicialmente observada. As duas enzimas produzidas apresentaram propriedades desejáveis em diferentes aplicações industriais, como relativa termoestabilidade e atuação em uma ampla faixa de pH. Assim, o pelo de cachorro é um resíduo promissor para produção de proteases alcalinas, nas duas colorações testadas, por ser um substrato de baixo custo e gerado em grandes quantidades por pet shops no mundo todo.Made available in DSpace on 2016-09-20T12:29:25Z (GMT). No. of bitstreams: 1 PR THAIANE PROLO.pdf: 2256156 bytes, checksum: 3cfd3dce47ffbd202848afffe03a563e (MD5) Previous issue date: 2015-02-13Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfhttp://localhost:8080/tede/retrieve/1185/PR%20THAIANE%20PROLO.pdf.jpgporUNICENTRO - Universidade Estadual do Centro OestePrograma de Pós-Graduação em Biologia Evolutiva (Mestrado)UNICENTROBRUnicentro::Departamento de BiologiaProtease alcalinapelo de cachorrolinhagens fúngicasmetodologia de superfície de respostaAlkaline proteasedog hairfungal strainsresponse surface methodologyCIENCIAS BIOLOGICAS::BIOLOGIA GERALBIOPROSPECÇÃO DE FUNGOS PRODUTORES DE PROTEASES: POTENCIAL PROTEOLÍTICO DE Fusarium oxysporumnão constainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis18315802413433703826006006006006887173787787986718-16345593859312446972075167498588264571info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UNICENTROinstname:Universidade Estadual do Centro-Oeste (UNICENTRO)instacron:UNICENTROORIGINALPR THAIANE PROLO.pdfapplication/pdf2256156http://localhost:8080/tede/bitstream/tede/393/1/PR+THAIANE+PROLO.pdf3cfd3dce47ffbd202848afffe03a563eMD51TEXTPR THAIANE PROLO.pdf.txtPR THAIANE PROLO.pdf.txttext/plain191149http://localhost:8080/tede/bitstream/tede/393/2/PR+THAIANE+PROLO.pdf.txt537b0e9cbfc27eebb5f36e26a024081cMD52THUMBNAILPR THAIANE PROLO.pdf.jpgPR THAIANE PROLO.pdf.jpgimage/jpeg3328http://localhost:8080/tede/bitstream/tede/393/3/PR+THAIANE+PROLO.pdf.jpgf18fe0f483823c61c84c5e944e8c044dMD53tede/3932017-09-12 09:39:56.109oai:localhost:tede/393Biblioteca Digital de Teses e Dissertaçõeshttp://tede.unicentro.br:8080/jspui/PUBhttp://tede.unicentro.br/tde_oai/oai3.phprepositorio@unicentro.br||fabianoqueiroz@yahoo.com.bropendoar:2017-09-12T12:39:56Biblioteca Digital de Teses e Dissertações do UNICENTRO - Universidade Estadual do Centro-Oeste (UNICENTRO)false
dc.title.por.fl_str_mv BIOPROSPECÇÃO DE FUNGOS PRODUTORES DE PROTEASES: POTENCIAL PROTEOLÍTICO DE Fusarium oxysporum
dc.title.alternative.eng.fl_str_mv não consta
title BIOPROSPECÇÃO DE FUNGOS PRODUTORES DE PROTEASES: POTENCIAL PROTEOLÍTICO DE Fusarium oxysporum
spellingShingle BIOPROSPECÇÃO DE FUNGOS PRODUTORES DE PROTEASES: POTENCIAL PROTEOLÍTICO DE Fusarium oxysporum
Prolo, Thaiane
Protease alcalina
pelo de cachorro
linhagens fúngicas
metodologia de superfície de resposta
Alkaline protease
dog hair
fungal strains
response surface methodology
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
title_short BIOPROSPECÇÃO DE FUNGOS PRODUTORES DE PROTEASES: POTENCIAL PROTEOLÍTICO DE Fusarium oxysporum
title_full BIOPROSPECÇÃO DE FUNGOS PRODUTORES DE PROTEASES: POTENCIAL PROTEOLÍTICO DE Fusarium oxysporum
title_fullStr BIOPROSPECÇÃO DE FUNGOS PRODUTORES DE PROTEASES: POTENCIAL PROTEOLÍTICO DE Fusarium oxysporum
title_full_unstemmed BIOPROSPECÇÃO DE FUNGOS PRODUTORES DE PROTEASES: POTENCIAL PROTEOLÍTICO DE Fusarium oxysporum
title_sort BIOPROSPECÇÃO DE FUNGOS PRODUTORES DE PROTEASES: POTENCIAL PROTEOLÍTICO DE Fusarium oxysporum
author Prolo, Thaiane
author_facet Prolo, Thaiane
author_role author
dc.contributor.advisor1.fl_str_mv Knob, Adriana
dc.contributor.advisor1ID.fl_str_mv CPF:00101001001
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/1289646338155108
dc.contributor.advisor-co1.fl_str_mv Lima, Vanderlei Aparecido de
dc.contributor.advisor-co1ID.fl_str_mv CPF:00000000104
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/9090461949264421
dc.contributor.authorID.fl_str_mv CPF:94658412268
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/7686288883788281
dc.contributor.author.fl_str_mv Prolo, Thaiane
contributor_str_mv Knob, Adriana
Lima, Vanderlei Aparecido de
dc.subject.por.fl_str_mv Protease alcalina
pelo de cachorro
linhagens fúngicas
metodologia de superfície de resposta
topic Protease alcalina
pelo de cachorro
linhagens fúngicas
metodologia de superfície de resposta
Alkaline protease
dog hair
fungal strains
response surface methodology
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
dc.subject.eng.fl_str_mv Alkaline protease
dog hair
fungal strains
response surface methodology
dc.subject.cnpq.fl_str_mv CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
description The proteolytic enzymes are one of the most important groups of commercial enzymes, representing about 60% of the global market for enzymes. Among the biotechnological applications of proteases, can be mentioned their application in detergents, leather, food, and other industries. The objective of this study was selection fungal that was better protease producers. To this, 73 fungal species were evaluated, 35 yeasts and 38 filamentous fungi. After an initial selection, the effect of various physico-chemical parameters on proteases production by the strain previously selected and subsequently identified as Fusarium oxysporum was investigated, using dog hair in white and black colors as substrates. The experiments were performed using Central composite rotational design applicable to Response Surface Methodology. Proteases produced were biochemically characterized, determining the following parameters: optimum temperature, optimum pH, thermostability, stability at different pH and the influence of ions and substances. Among the evaluated fungal strains, F. oxysporum produced the highest levels of alkaline protease when its was grown in black dog hair. Maximum production levels were achieved when the pH of medium was adjusted to 4.2, substrate concentration was 4.2% and the cultivation time was 8.6 days, corresponding to 72.8 U mL?¹. When it was grown in white dog hair, maximum production levels were obtained in pH of medium adjusted to 6.0, on substrate concentration of 5.0% and cultivation time of 6.5 days, corresponding to 51.84 U mL?¹. Productivity levels obtained employing white and black dog hair as substrates correspond an increase of 14.85 and 7.19 fold, respectively, in relation to the initial activity observed. Both enzymes exhibited desirable properties to be apply in many industries, such as relative thermal stability and action in a wide pH range. Thus, dog hair is a promising residue for alkaline proteases production, in both colors that were tested, due to be a low cost material and generated in large amounts by pet shops throughout the world.
publishDate 2015
dc.date.available.fl_str_mv 2015-11-23
dc.date.issued.fl_str_mv 2015-02-13
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dc.identifier.uri.fl_str_mv http://localhost:8080/tede/handle/tede/393
identifier_str_mv PROLO, Thaiane. não consta. 2015. 101 f. Dissertação (Mestrado em Biologia Evolutiva) - UNICENTRO - Universidade Estadual do Centro Oeste, Guarapuava, 2015.
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