HIDRÓLISE DO AMIDO DA BATATA DOCE POR ENZIMAS PURIFICADAS E PRODUZIDAS POR Aspergillus niger EM CONDIÇÕES OTIMIZADAS
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações do UNICENTRO |
Texto Completo: | http://localhost:8080/tede/handle/tede/27 |
Resumo: | The sweet potato [Ipomoea batatas (L) Lam] presents itself as an excellent source of biomass for ethanol production, coupled with low production cost and hardiness, but not economically viable due to high costs in the process of hydrolysis. The activities developed in this study aimed to optimize the production of starch enzymes produced by Aspergillus niger and later use them in the hydrolytic process in five cultivars of sweet potato, as well as to compare the efficiency of these commercial enzymes. In order to establish levels of amylases higher, were evaluated under different conditions of cultivation of the fungus such as a carbon source, pH of the culture medium, kinetics and growth temperature. Subsequently, the best conditions to hydrolytic sweet potatoes were both determined using purified enzymes (?-amylase and amyloglucosidase) or produced by A. niger. The reducing sugars were quantified by ADNS (Miller, 1959) and the glucose content by using enzymatic kit glucose. Excellent production levels of amylase are obtained when A. niger was grown in cassava flour, for five days, pH 5.0 and 30ºC. Improved efficiency of the process of cultivating hydrolytic UGA 5 to the cost / benefit of employing commercial enzymes was obtained through the association of ?-amylase and amyloglucosidase purified in concentrations of 0,5 U/g starch and 750 U/g starch , respectively, for 120 minutes. By employing the enzymes produced by A. niger best hydrolysis conditions established for the same genotype were six hours of reaction at 60°C and pH 4,5, resulting in the release of reducing sugars of 0,76 g and 0,53 g of glucose from 1 g of sweet potato. After the optimization, the hydrolytic process was evaluated on all cultivars. Among them, UGA56 showed the highest yields, but UGA5 was more promising, due to its higher productivity and good rates of saccharification, corresponding to 0,77 g of glucose to purified enzymes and 0,53 g for fungal enzymes, implying , stoichiometrically in 50 ml of ethanol and 34. The bioprocess using A. niger as a producer of amylases were satisfactory, allowing good conversion of sweet potato starch into sugars, and especially a considerable reduction in costs associated with the process of saccharification. |
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Resende, Juliano Tadeu Vilela deCPF:12587445997http://lattes.cnpq.br/8328754543048690Knob, AdrianaCPF:00101001001http://lattes.cnpq.br/1289646338155108CPF:04742310977http://lattes.cnpq.br/8314006400978402Stroparo, Erivelton César2016-09-20T12:11:28Z2015-05-112012-01-01STROPARO, Erivelton César. Starch hydrolysis of potato and sweet for purified enzymes produced by Aspergillus niger under optimized conditions. 2012. 98 f. Dissertação (Mestrado em Biocombustiveis) - UNICENTRO - Universidade Estadual do Centro Oeste, Guarapuava, 2012.http://localhost:8080/tede/handle/tede/27The sweet potato [Ipomoea batatas (L) Lam] presents itself as an excellent source of biomass for ethanol production, coupled with low production cost and hardiness, but not economically viable due to high costs in the process of hydrolysis. The activities developed in this study aimed to optimize the production of starch enzymes produced by Aspergillus niger and later use them in the hydrolytic process in five cultivars of sweet potato, as well as to compare the efficiency of these commercial enzymes. In order to establish levels of amylases higher, were evaluated under different conditions of cultivation of the fungus such as a carbon source, pH of the culture medium, kinetics and growth temperature. Subsequently, the best conditions to hydrolytic sweet potatoes were both determined using purified enzymes (?-amylase and amyloglucosidase) or produced by A. niger. The reducing sugars were quantified by ADNS (Miller, 1959) and the glucose content by using enzymatic kit glucose. Excellent production levels of amylase are obtained when A. niger was grown in cassava flour, for five days, pH 5.0 and 30ºC. Improved efficiency of the process of cultivating hydrolytic UGA 5 to the cost / benefit of employing commercial enzymes was obtained through the association of ?-amylase and amyloglucosidase purified in concentrations of 0,5 U/g starch and 750 U/g starch , respectively, for 120 minutes. By employing the enzymes produced by A. niger best hydrolysis conditions established for the same genotype were six hours of reaction at 60°C and pH 4,5, resulting in the release of reducing sugars of 0,76 g and 0,53 g of glucose from 1 g of sweet potato. After the optimization, the hydrolytic process was evaluated on all cultivars. Among them, UGA56 showed the highest yields, but UGA5 was more promising, due to its higher productivity and good rates of saccharification, corresponding to 0,77 g of glucose to purified enzymes and 0,53 g for fungal enzymes, implying , stoichiometrically in 50 ml of ethanol and 34. The bioprocess using A. niger as a producer of amylases were satisfactory, allowing good conversion of sweet potato starch into sugars, and especially a considerable reduction in costs associated with the process of saccharification.A batata-doce [Ipomoea batatas (L) Lam] apresenta-se como uma excelente fonte de biomassa para produção de álcool combustível, associada a baixo custo de produção e rusticidade, porém inviável economicamente devido a altos custos em seu processo de hidrólise. As atividades desenvolvidas neste estudo visaram otimizar a produção de enzimas amiláceas produzidas por Aspergillus niger e posteriormente utilizá-las no processo hidrolítico de cinco cultivares de batata-doce, bem como comparar a eficiência destas com enzimas comerciais. A fim de se estabelecer níveis de produção de amilases mais elevados, foram avaliadas diferentes condições de cultivo do fungo tais como fonte de carbono, pH do meio de cultivo, cinética e temperatura de crescimento. Posteriormente, as melhores condições hidrolíticas para batata-doce foram determinadas utilizando-se tanto enzimas purificadas (?-amilase e amiloglicosidase) quanto produzidas por A. niger. Os açúcares redutores totais foram quantificados pelo método ADNS (Miller, 1959) e o teor de glicose por meio da utilização do kit glicose método enzimático. Excelentes níveis de produção de amilases foram obtidos quando A. niger foi cultivado em farinha de mandioca, por cinco dias, pH 5,0 e a temperatura de 30°C. Maior eficiência do processo hidrolitico da cultivar UGA 5 em relação ao custo/benefício empregando-se enzimas comerciais foi obtida por meio da associação de amiloglicosidase e ?-amilase purificadas, nas concentrações de 0,5 U/g amido e 750 U/g amido, respectivamente, por 120 minutos. Ao se empregar as enzimas produzidas por A. niger, as melhores condições de hidrólise estabelecidas para este mesmo cultivar foram seis horas de reação, temperatura de 60ºC e pH 4,5, resultando na liberação de 0,76 g de açúcares redutores e 0,53 g de glicose, a partir de 1 g de batata-doce. Após sua otimização, o processo hidrolítico foi avaliado sobre todas as cultivares. Dentre elas, a UGA56 apresentou os maiores rendimentos, porém a UGA5 mostrou-se mais promissora, por apresentar maior produtividade e bons índices de sacarificação, correspondendo a 0,77 g de glicose para enzimas purificadas e 0,53 g para enzimas fúngicas, implicando, estequiometricamente em 50 e 34 ml de etanol. O bioprocesso utilizando A. niger como produtor de amilases se mostrou satisfatório, possibilitando boa conversão do amido de batata-doce em açúcares e, principalmente, uma considerável redução nos custos associados ao processo de sacarificação.Made available in DSpace on 2016-09-20T12:11:28Z (GMT). No. of bitstreams: 1 PR ERIVELTON CESAR STROPARO.pdf: 4749848 bytes, checksum: a4423e46091edb804b79f68903be4fb1 (MD5) Previous issue date: 2012-01-01Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfhttp://localhost:8080/tede/retrieve/630/PR%20ERIVELTON%20CESAR%20STROPARO.pdf.jpgporUNICENTRO - Universidade Estadual do Centro OestePrograma de Pós-Graduação em Bioenergia (Mestrado)UNICENTROBRUnicentro::Departamento de Ciências Agrárias e AmbientaisAmilasesAspergillus nigerBatata-doceHidrólise enzimáticaAmylasesAspergillus nigerSweet potatoEnzymatic hydrolysisENGENHARIA BIOMEDICA::BIOENGENHARIAHIDRÓLISE DO AMIDO DA BATATA DOCE POR ENZIMAS PURIFICADAS E PRODUZIDAS POR Aspergillus niger EM CONDIÇÕES OTIMIZADASStarch hydrolysis of potato and sweet for purified enzymes produced by Aspergillus niger under optimized conditionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-2202725866144043770600600600600-7661102638863479717-43760724932823322272075167498588264571info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UNICENTROinstname:Universidade Estadual do Centro-Oeste (UNICENTRO)instacron:UNICENTROORIGINALPR ERIVELTON CESAR STROPARO.pdfapplication/pdf4749848http://localhost:8080/tede/bitstream/tede/27/1/PR+ERIVELTON+CESAR+STROPARO.pdfa4423e46091edb804b79f68903be4fb1MD51THUMBNAILPR ERIVELTON CESAR STROPARO.pdf.jpgPR ERIVELTON CESAR STROPARO.pdf.jpgimage/jpeg3191http://localhost:8080/tede/bitstream/tede/27/2/PR+ERIVELTON+CESAR+STROPARO.pdf.jpg98c0f336952efcd3ddf710ceed81335eMD52tede/272019-06-05 16:28:04.534oai:localhost:tede/27Biblioteca Digital de Teses e Dissertaçõeshttp://tede.unicentro.br:8080/jspui/PUBhttp://tede.unicentro.br/tde_oai/oai3.phprepositorio@unicentro.br||fabianoqueiroz@yahoo.com.bropendoar:2019-06-05T19:28:04Biblioteca Digital de Teses e Dissertações do UNICENTRO - Universidade Estadual do Centro-Oeste (UNICENTRO)false |
dc.title.por.fl_str_mv |
HIDRÓLISE DO AMIDO DA BATATA DOCE POR ENZIMAS PURIFICADAS E PRODUZIDAS POR Aspergillus niger EM CONDIÇÕES OTIMIZADAS |
dc.title.alternative.eng.fl_str_mv |
Starch hydrolysis of potato and sweet for purified enzymes produced by Aspergillus niger under optimized conditions |
title |
HIDRÓLISE DO AMIDO DA BATATA DOCE POR ENZIMAS PURIFICADAS E PRODUZIDAS POR Aspergillus niger EM CONDIÇÕES OTIMIZADAS |
spellingShingle |
HIDRÓLISE DO AMIDO DA BATATA DOCE POR ENZIMAS PURIFICADAS E PRODUZIDAS POR Aspergillus niger EM CONDIÇÕES OTIMIZADAS Stroparo, Erivelton César Amilases Aspergillus niger Batata-doce Hidrólise enzimática Amylases Aspergillus niger Sweet potato Enzymatic hydrolysis ENGENHARIA BIOMEDICA::BIOENGENHARIA |
title_short |
HIDRÓLISE DO AMIDO DA BATATA DOCE POR ENZIMAS PURIFICADAS E PRODUZIDAS POR Aspergillus niger EM CONDIÇÕES OTIMIZADAS |
title_full |
HIDRÓLISE DO AMIDO DA BATATA DOCE POR ENZIMAS PURIFICADAS E PRODUZIDAS POR Aspergillus niger EM CONDIÇÕES OTIMIZADAS |
title_fullStr |
HIDRÓLISE DO AMIDO DA BATATA DOCE POR ENZIMAS PURIFICADAS E PRODUZIDAS POR Aspergillus niger EM CONDIÇÕES OTIMIZADAS |
title_full_unstemmed |
HIDRÓLISE DO AMIDO DA BATATA DOCE POR ENZIMAS PURIFICADAS E PRODUZIDAS POR Aspergillus niger EM CONDIÇÕES OTIMIZADAS |
title_sort |
HIDRÓLISE DO AMIDO DA BATATA DOCE POR ENZIMAS PURIFICADAS E PRODUZIDAS POR Aspergillus niger EM CONDIÇÕES OTIMIZADAS |
author |
Stroparo, Erivelton César |
author_facet |
Stroparo, Erivelton César |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Resende, Juliano Tadeu Vilela de |
dc.contributor.advisor1ID.fl_str_mv |
CPF:12587445997 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/8328754543048690 |
dc.contributor.advisor-co1.fl_str_mv |
Knob, Adriana |
dc.contributor.advisor-co1ID.fl_str_mv |
CPF:00101001001 |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/1289646338155108 |
dc.contributor.authorID.fl_str_mv |
CPF:04742310977 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/8314006400978402 |
dc.contributor.author.fl_str_mv |
Stroparo, Erivelton César |
contributor_str_mv |
Resende, Juliano Tadeu Vilela de Knob, Adriana |
dc.subject.por.fl_str_mv |
Amilases Aspergillus niger Batata-doce Hidrólise enzimática |
topic |
Amilases Aspergillus niger Batata-doce Hidrólise enzimática Amylases Aspergillus niger Sweet potato Enzymatic hydrolysis ENGENHARIA BIOMEDICA::BIOENGENHARIA |
dc.subject.eng.fl_str_mv |
Amylases Aspergillus niger Sweet potato Enzymatic hydrolysis |
dc.subject.cnpq.fl_str_mv |
ENGENHARIA BIOMEDICA::BIOENGENHARIA |
description |
The sweet potato [Ipomoea batatas (L) Lam] presents itself as an excellent source of biomass for ethanol production, coupled with low production cost and hardiness, but not economically viable due to high costs in the process of hydrolysis. The activities developed in this study aimed to optimize the production of starch enzymes produced by Aspergillus niger and later use them in the hydrolytic process in five cultivars of sweet potato, as well as to compare the efficiency of these commercial enzymes. In order to establish levels of amylases higher, were evaluated under different conditions of cultivation of the fungus such as a carbon source, pH of the culture medium, kinetics and growth temperature. Subsequently, the best conditions to hydrolytic sweet potatoes were both determined using purified enzymes (?-amylase and amyloglucosidase) or produced by A. niger. The reducing sugars were quantified by ADNS (Miller, 1959) and the glucose content by using enzymatic kit glucose. Excellent production levels of amylase are obtained when A. niger was grown in cassava flour, for five days, pH 5.0 and 30ºC. Improved efficiency of the process of cultivating hydrolytic UGA 5 to the cost / benefit of employing commercial enzymes was obtained through the association of ?-amylase and amyloglucosidase purified in concentrations of 0,5 U/g starch and 750 U/g starch , respectively, for 120 minutes. By employing the enzymes produced by A. niger best hydrolysis conditions established for the same genotype were six hours of reaction at 60°C and pH 4,5, resulting in the release of reducing sugars of 0,76 g and 0,53 g of glucose from 1 g of sweet potato. After the optimization, the hydrolytic process was evaluated on all cultivars. Among them, UGA56 showed the highest yields, but UGA5 was more promising, due to its higher productivity and good rates of saccharification, corresponding to 0,77 g of glucose to purified enzymes and 0,53 g for fungal enzymes, implying , stoichiometrically in 50 ml of ethanol and 34. The bioprocess using A. niger as a producer of amylases were satisfactory, allowing good conversion of sweet potato starch into sugars, and especially a considerable reduction in costs associated with the process of saccharification. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-01-01 |
dc.date.available.fl_str_mv |
2015-05-11 |
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2016-09-20T12:11:28Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
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STROPARO, Erivelton César. Starch hydrolysis of potato and sweet for purified enzymes produced by Aspergillus niger under optimized conditions. 2012. 98 f. Dissertação (Mestrado em Biocombustiveis) - UNICENTRO - Universidade Estadual do Centro Oeste, Guarapuava, 2012. |
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http://localhost:8080/tede/handle/tede/27 |
identifier_str_mv |
STROPARO, Erivelton César. Starch hydrolysis of potato and sweet for purified enzymes produced by Aspergillus niger under optimized conditions. 2012. 98 f. Dissertação (Mestrado em Biocombustiveis) - UNICENTRO - Universidade Estadual do Centro Oeste, Guarapuava, 2012. |
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UNICENTRO |
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UNICENTRO - Universidade Estadual do Centro Oeste |
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