FUNÇÃO ESPERMÁTICA E REPRODUTIVA EM RATOS WISTAR EXPOSTOS AO BISFENOL A NO PERÍODO PRÉ-ADULTO
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações do UNICENTRO |
Texto Completo: | http://tede.unicentro.br:8080/jspui/handle/jspui/666 |
Resumo: | Bisphenol A (BPA) is a monomer of polycarbonate plastic widely used in industry for the manufacture of packaging, epoxy resins and other materials. There are many effects previously seen as caused by BPA, among them testicular toxicity, reproductive changes in animal models and reports of human exposure to the chemical. The incomplete polymerization of BPA based products during manufacture and depolymerization induced by temperature’s change cause release of the compound and its derivatives into the environment and can be detected in canned foods, bottled liquids, in the air we breathe, and contaminant of rivers. In this research, we propose to test the hypothesis that BPA disrupts endocrine and reproductive systems. We evaluate the possible changes in 30 male wistar rats, in pre-adult phase, exposed for 40 days to 0mg/kg, 5mg/kg and 25mg/kg of BPA, and observe the evolution of body weight; testis and epididymis (divided into portions head, body and tail) weight; the mean concentration of sperm in the sample collected and sperm pathologies; the functionality of spermatozoon by observing mitochondrial activity, plasma membrane and acrosomal integrity; damage to the genetic material of the sperm; evaluation of sperm production, sperm reserves and sperm transit; we evaluated the sexual behavior and sexual orientation; and the hormonal profile in LH, FSH, testosterone and estradiol. The results observed were judged by statistical methods such as analysis of variance and comparison of means; Kruskal-Wallis test with comparisons by Freedman and Mann-Whitney tests. The parametric values were expressed as mean and standard error of the mean, and nonparametric data, as median and interquartile ranges. Statistical difference was considered when the p value was less than 0,05. We demonstrate that the product block the evolution of body weight; it alters the relative weight of the head and tail of the epididymis; it decreases the sperm concentration; it doesn’t alter morphology; it decreases mitochondrial activity in the sperm midpiece; it declines plasma membrane integrity of the sperm and decreases acrosome integrity; it does not cause changes in sperm DNA; it alters sexual orientation, by decreasing the preference for receptive females; it alters sexual behavior by decreasing latency to the first mount and intromission, and the number of ejaculations in a period; it declines daily sperm production and efficiency of spermatogenesis; it decreases sperm reserves; it slows the transit of epididymal spermatozoa; it declines hormones LH, FSH and testosterone in serum, and increases the hormone estradiol. We concluded that the product caused demasculinization of treated animals. |
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Romano, Marco Auréliohttp://lattes.cnpq.br/1024434825015654Romano, Marco Aurélio010.313.439-54http://lattes.cnpq.br/5881506413666847WISNIEWSKI, PATRICIA2017-06-05T17:48:01Z2013-11-22WISNIEWSKI, PATRICIA. FUNÇÃO ESPERMÁTICA E REPRODUTIVA EM RATOS WISTAR EXPOSTOS AO BISFENOL A NO PERÍODO PRÉ-ADULTO. 2013. 78 f. Dissertação (Programa de Pós-Graduação em Ciências Farmacêuticas - Mestrado / Associação Ampla com UEPG) - Universidade Estadual do Centro-Oeste, Guarapuava - PR.http://tede.unicentro.br:8080/jspui/handle/jspui/666Bisphenol A (BPA) is a monomer of polycarbonate plastic widely used in industry for the manufacture of packaging, epoxy resins and other materials. There are many effects previously seen as caused by BPA, among them testicular toxicity, reproductive changes in animal models and reports of human exposure to the chemical. The incomplete polymerization of BPA based products during manufacture and depolymerization induced by temperature’s change cause release of the compound and its derivatives into the environment and can be detected in canned foods, bottled liquids, in the air we breathe, and contaminant of rivers. In this research, we propose to test the hypothesis that BPA disrupts endocrine and reproductive systems. We evaluate the possible changes in 30 male wistar rats, in pre-adult phase, exposed for 40 days to 0mg/kg, 5mg/kg and 25mg/kg of BPA, and observe the evolution of body weight; testis and epididymis (divided into portions head, body and tail) weight; the mean concentration of sperm in the sample collected and sperm pathologies; the functionality of spermatozoon by observing mitochondrial activity, plasma membrane and acrosomal integrity; damage to the genetic material of the sperm; evaluation of sperm production, sperm reserves and sperm transit; we evaluated the sexual behavior and sexual orientation; and the hormonal profile in LH, FSH, testosterone and estradiol. The results observed were judged by statistical methods such as analysis of variance and comparison of means; Kruskal-Wallis test with comparisons by Freedman and Mann-Whitney tests. The parametric values were expressed as mean and standard error of the mean, and nonparametric data, as median and interquartile ranges. Statistical difference was considered when the p value was less than 0,05. We demonstrate that the product block the evolution of body weight; it alters the relative weight of the head and tail of the epididymis; it decreases the sperm concentration; it doesn’t alter morphology; it decreases mitochondrial activity in the sperm midpiece; it declines plasma membrane integrity of the sperm and decreases acrosome integrity; it does not cause changes in sperm DNA; it alters sexual orientation, by decreasing the preference for receptive females; it alters sexual behavior by decreasing latency to the first mount and intromission, and the number of ejaculations in a period; it declines daily sperm production and efficiency of spermatogenesis; it decreases sperm reserves; it slows the transit of epididymal spermatozoa; it declines hormones LH, FSH and testosterone in serum, and increases the hormone estradiol. We concluded that the product caused demasculinization of treated animals.O bisfenol A (BPA) é um monômero do plástico policarbonato amplamente utilizado na indústria para fabricação de embalagens, resinas epóxi e outros materiais. Vários são os efeitos já evidenciados como causados pelo BPA, entre eles toxicidade testicular e alterações reprodutivas em modelos animais, e relatos da exposição humana ao químico. A polimerização incompleta dos produtos à base de BPA durante a fabricação, e a despolimerização induzida por alteração de temperatura causam a liberação do composto e de seus derivados para o meio, podendo ser detectados em alimentos enlatados, líquidos engarrafados, no ar que respiramos e como contaminante de rios. Neste trabalho, propôs-se testar a hipótese de que o BPA desregula o sistema endócrino e reprodutivo. Avaliou-se as possíveis alterações em 30 ratos wistar, em idade pré-adulta, expostos por 40 dias a 0mg/kg, 5mg/kg e 25mg/kg de BPA. Observou-se a evolução do peso corporal, peso do testículo e epidídimo (dividido nas porções cabeça, corpo e cauda); avaliou-se a concentração média de espermatozoides na amostra coletada; análise de patologias espermáticas; análise funcional, avaliada pela coloração com DAB, observando a atividade mitocondrial indiretamente, integridade de membrana plasmática pela coloração com Eosina/Nigrosina, e integridade acrossomal, pela coloração de Pope; pela avaliação do dano ao material genético do espermatozoide; avaliação da produção espermática, reservas espermáticas e tempo de trânsito espermático; avaliação do comportamento sexual e orientação sexual; e, pelo perfil hormonal avaliado em LH, FSH, testosterona e estradiol. Os resultados observados no estudo foram avaliados por análise de variância e os testes de comparação entre médias, e, teste de Kruskall-Wallis com as comparações pelos testes de Freedman e Mann-Whitney. Os valores paramétricos foram expressos em média e erro-padrão da média e, para dados não paramétricos, em mediana e intervalos interquartílicos. Considerou-se diferença estatística quando o valor de p foi inferior a 0,05. Demonstrou-se que o produto dificulta a evolução do peso corporal; altera o peso relativo de cabeça e cauda de epidídimo; diminui concentração de espermatozoides; não altera morfologia espermática; diminui atividade mitocondrial na peça intermediária do espermatozoide; diminui integridade de membrana plasmática do espermatozoide e diminui integridade de acrossoma; não causa alteração no DNA espermático; altera a orientação sexual, por diminuir a preferência por cobaias fêmeas receptivas; altera o comportamento sexual, diminuindo a latência para primeira monta e intromissão, e o número de ejaculações em um período determinado; diminui a produção diária de espermatozoides e a eficiência da espermatogênese; diminui a reserva espermática; retarda o trânsito epididimário dos espermatozoides; diminui os hormônios LH, FSH e testosterona; e, aumenta o hormônio estradiol no soro. Conclui-se que o produto causou desmasculinização dos animais tratados.Submitted by Fabiano Jucá (fjuca@unicentro.br) on 2017-06-05T17:48:01Z No. of bitstreams: 1 PATRICIA WISNIEWSKI.pdf: 1415780 bytes, checksum: d29b762d6311e497c235f9f9264b5756 (MD5)Made available in DSpace on 2017-06-05T17:48:01Z (GMT). No. of bitstreams: 1 PATRICIA WISNIEWSKI.pdf: 1415780 bytes, checksum: d29b762d6311e497c235f9f9264b5756 (MD5) Previous issue date: 2013-11-22Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfhttp://tede.unicentro.br:8080/jspui/retrieve/2026/PATRICIA%20WISNIEWSKI.pdf.jpgporUniversidade Estadual do Centro-OestePrograma de Pós-Graduação em Ciências Farmacêuticas (Mestrado / Associação Ampla com UEPG)UNICENTROBrasilUnicentro::Departamento de Farmácia2,2-bis(4-hidroxifenil)propanoReproduçãoFuncionalidade espermática2,2-bis(4-hydroxyphenyl)propaneReproductionSperm functionalityCIENCIAS DA SAUDE::FARMACIAFUNÇÃO ESPERMÁTICA E REPRODUTIVA EM RATOS WISTAR EXPOSTOS AO BISFENOL A NO PERÍODO PRÉ-ADULTOinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-7679163762264962259600600600600777590143274119112569976364134497549962075167498588264571info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UNICENTROinstname:Universidade Estadual do Centro-Oeste (UNICENTRO)instacron:UNICENTROLICENSElicense.txtlicense.txttext/plain; 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dc.title.por.fl_str_mv |
FUNÇÃO ESPERMÁTICA E REPRODUTIVA EM RATOS WISTAR EXPOSTOS AO BISFENOL A NO PERÍODO PRÉ-ADULTO |
title |
FUNÇÃO ESPERMÁTICA E REPRODUTIVA EM RATOS WISTAR EXPOSTOS AO BISFENOL A NO PERÍODO PRÉ-ADULTO |
spellingShingle |
FUNÇÃO ESPERMÁTICA E REPRODUTIVA EM RATOS WISTAR EXPOSTOS AO BISFENOL A NO PERÍODO PRÉ-ADULTO WISNIEWSKI, PATRICIA 2,2-bis(4-hidroxifenil)propano Reprodução Funcionalidade espermática 2,2-bis(4-hydroxyphenyl)propane Reproduction Sperm functionality CIENCIAS DA SAUDE::FARMACIA |
title_short |
FUNÇÃO ESPERMÁTICA E REPRODUTIVA EM RATOS WISTAR EXPOSTOS AO BISFENOL A NO PERÍODO PRÉ-ADULTO |
title_full |
FUNÇÃO ESPERMÁTICA E REPRODUTIVA EM RATOS WISTAR EXPOSTOS AO BISFENOL A NO PERÍODO PRÉ-ADULTO |
title_fullStr |
FUNÇÃO ESPERMÁTICA E REPRODUTIVA EM RATOS WISTAR EXPOSTOS AO BISFENOL A NO PERÍODO PRÉ-ADULTO |
title_full_unstemmed |
FUNÇÃO ESPERMÁTICA E REPRODUTIVA EM RATOS WISTAR EXPOSTOS AO BISFENOL A NO PERÍODO PRÉ-ADULTO |
title_sort |
FUNÇÃO ESPERMÁTICA E REPRODUTIVA EM RATOS WISTAR EXPOSTOS AO BISFENOL A NO PERÍODO PRÉ-ADULTO |
author |
WISNIEWSKI, PATRICIA |
author_facet |
WISNIEWSKI, PATRICIA |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Romano, Marco Aurélio |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1024434825015654 |
dc.contributor.advisor2.fl_str_mv |
Romano, Marco Aurélio |
dc.contributor.authorID.fl_str_mv |
010.313.439-54 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5881506413666847 |
dc.contributor.author.fl_str_mv |
WISNIEWSKI, PATRICIA |
contributor_str_mv |
Romano, Marco Aurélio Romano, Marco Aurélio |
dc.subject.por.fl_str_mv |
2,2-bis(4-hidroxifenil)propano Reprodução Funcionalidade espermática |
topic |
2,2-bis(4-hidroxifenil)propano Reprodução Funcionalidade espermática 2,2-bis(4-hydroxyphenyl)propane Reproduction Sperm functionality CIENCIAS DA SAUDE::FARMACIA |
dc.subject.eng.fl_str_mv |
2,2-bis(4-hydroxyphenyl)propane Reproduction Sperm functionality |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::FARMACIA |
description |
Bisphenol A (BPA) is a monomer of polycarbonate plastic widely used in industry for the manufacture of packaging, epoxy resins and other materials. There are many effects previously seen as caused by BPA, among them testicular toxicity, reproductive changes in animal models and reports of human exposure to the chemical. The incomplete polymerization of BPA based products during manufacture and depolymerization induced by temperature’s change cause release of the compound and its derivatives into the environment and can be detected in canned foods, bottled liquids, in the air we breathe, and contaminant of rivers. In this research, we propose to test the hypothesis that BPA disrupts endocrine and reproductive systems. We evaluate the possible changes in 30 male wistar rats, in pre-adult phase, exposed for 40 days to 0mg/kg, 5mg/kg and 25mg/kg of BPA, and observe the evolution of body weight; testis and epididymis (divided into portions head, body and tail) weight; the mean concentration of sperm in the sample collected and sperm pathologies; the functionality of spermatozoon by observing mitochondrial activity, plasma membrane and acrosomal integrity; damage to the genetic material of the sperm; evaluation of sperm production, sperm reserves and sperm transit; we evaluated the sexual behavior and sexual orientation; and the hormonal profile in LH, FSH, testosterone and estradiol. The results observed were judged by statistical methods such as analysis of variance and comparison of means; Kruskal-Wallis test with comparisons by Freedman and Mann-Whitney tests. The parametric values were expressed as mean and standard error of the mean, and nonparametric data, as median and interquartile ranges. Statistical difference was considered when the p value was less than 0,05. We demonstrate that the product block the evolution of body weight; it alters the relative weight of the head and tail of the epididymis; it decreases the sperm concentration; it doesn’t alter morphology; it decreases mitochondrial activity in the sperm midpiece; it declines plasma membrane integrity of the sperm and decreases acrosome integrity; it does not cause changes in sperm DNA; it alters sexual orientation, by decreasing the preference for receptive females; it alters sexual behavior by decreasing latency to the first mount and intromission, and the number of ejaculations in a period; it declines daily sperm production and efficiency of spermatogenesis; it decreases sperm reserves; it slows the transit of epididymal spermatozoa; it declines hormones LH, FSH and testosterone in serum, and increases the hormone estradiol. We concluded that the product caused demasculinization of treated animals. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-11-22 |
dc.date.accessioned.fl_str_mv |
2017-06-05T17:48:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
WISNIEWSKI, PATRICIA. FUNÇÃO ESPERMÁTICA E REPRODUTIVA EM RATOS WISTAR EXPOSTOS AO BISFENOL A NO PERÍODO PRÉ-ADULTO. 2013. 78 f. Dissertação (Programa de Pós-Graduação em Ciências Farmacêuticas - Mestrado / Associação Ampla com UEPG) - Universidade Estadual do Centro-Oeste, Guarapuava - PR. |
dc.identifier.uri.fl_str_mv |
http://tede.unicentro.br:8080/jspui/handle/jspui/666 |
identifier_str_mv |
WISNIEWSKI, PATRICIA. FUNÇÃO ESPERMÁTICA E REPRODUTIVA EM RATOS WISTAR EXPOSTOS AO BISFENOL A NO PERÍODO PRÉ-ADULTO. 2013. 78 f. Dissertação (Programa de Pós-Graduação em Ciências Farmacêuticas - Mestrado / Associação Ampla com UEPG) - Universidade Estadual do Centro-Oeste, Guarapuava - PR. |
url |
http://tede.unicentro.br:8080/jspui/handle/jspui/666 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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dc.relation.confidence.fl_str_mv |
600 600 600 600 |
dc.relation.department.fl_str_mv |
7775901432741191125 |
dc.relation.cnpq.fl_str_mv |
6997636413449754996 |
dc.relation.sponsorship.fl_str_mv |
2075167498588264571 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual do Centro-Oeste |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciências Farmacêuticas (Mestrado / Associação Ampla com UEPG) |
dc.publisher.initials.fl_str_mv |
UNICENTRO |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Unicentro::Departamento de Farmácia |
publisher.none.fl_str_mv |
Universidade Estadual do Centro-Oeste |
dc.source.none.fl_str_mv |
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Biblioteca Digital de Teses e Dissertações do UNICENTRO |
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Biblioteca Digital de Teses e Dissertações do UNICENTRO |
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Biblioteca Digital de Teses e Dissertações do UNICENTRO - Universidade Estadual do Centro-Oeste (UNICENTRO) |
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repositorio@unicentro.br||fabianoqueiroz@yahoo.com.br |
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