Potencial Biotecnológico dos microrganismos endofíticos isolados de plantas de jambu (Acmella ciliata (Kunth) Cass.) para controle de microrganismos patógenos
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da Universidade do Estado do Amazonas (UEA) |
Texto Completo: | http://repositorioinstitucional.uea.edu.br//handle/riuea/2376 |
Resumo: | The Amazon rainforest has great biodiversity of species, including animals, plants and microorganisms. Many of these microorganisms are not yet known, as well as some of the potential uses and properties of these. Among these are the endophytic microorganisms who live inside the plants, many of them showing the same properties of the host. The plant “jambu” Acmella ciliata is known for its antimicrobial, antifungal properties among others, and is used in food and natural medicine in the Amazon region. For the isolation of endophytes, first held low-level disinfection of the twigs and leaves of the plant Acmella ciliata, sow and incubate the pieces of this plant material during 15 days at 28ºc. The isolated microorganisms were evaluated in trials of antagonism (Fusarium decemcellulare and Colletotrichum gloeosporioides) and antimicrobial activity (Streptococcus pyogenes and Candida parapsilosis). A total of 104 were isolated endophytic microorganisms (bacteria, filamentous fungi and yeasts), of this universe, 22 filamentous fungi and 16 bacteria showed inhibition of growth of plant pathogens, and six bacteria showed antimicrobial activity for Streptococcus pyogenes and Candida parapsilosis. Was shown that the endophytic microorganisms isolated from plant Acmella ciliata produce metabolites with antimicrobial and antifungal activity. |
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Potencial Biotecnológico dos microrganismos endofíticos isolados de plantas de jambu (Acmella ciliata (Kunth) Cass.) para controle de microrganismos patógenosendofíticojambubiotecnologiaThe Amazon rainforest has great biodiversity of species, including animals, plants and microorganisms. Many of these microorganisms are not yet known, as well as some of the potential uses and properties of these. Among these are the endophytic microorganisms who live inside the plants, many of them showing the same properties of the host. The plant “jambu” Acmella ciliata is known for its antimicrobial, antifungal properties among others, and is used in food and natural medicine in the Amazon region. For the isolation of endophytes, first held low-level disinfection of the twigs and leaves of the plant Acmella ciliata, sow and incubate the pieces of this plant material during 15 days at 28ºc. The isolated microorganisms were evaluated in trials of antagonism (Fusarium decemcellulare and Colletotrichum gloeosporioides) and antimicrobial activity (Streptococcus pyogenes and Candida parapsilosis). A total of 104 were isolated endophytic microorganisms (bacteria, filamentous fungi and yeasts), of this universe, 22 filamentous fungi and 16 bacteria showed inhibition of growth of plant pathogens, and six bacteria showed antimicrobial activity for Streptococcus pyogenes and Candida parapsilosis. Was shown that the endophytic microorganisms isolated from plant Acmella ciliata produce metabolites with antimicrobial and antifungal activity.A floresta amazônica possui grande biodiversidade de espécies, entre animais, plantas e microrganismos. Muitos destes microrganismos ainda não são conhecidos assim como os potenciais usos e propriedades destes. Entre estes se encontram os microrganismos endofíticos que habitam no interior das plantas, muitos deles apresentando as mesmas propriedades dos hospedeiros. A planta “jambu” Acmella ciliata é conhecida por suas propriedades antimicrobianas, antifúngicas entre outras, e é usada na gastronomia e na medicinal natural na região Amazônica. Para o isolamento dos endófitos, primeiro foi realizada a desinfecção superficial dos galhos e folhas da planta Acmella ciliata, para semear e incubar posteriormente os pedaços deste material vegetal durante 15 dias a 28ºC. Os microrganismos isolados foram avaliados em ensaios de antagonismo (Fusarium decemcellulare e Colletotrichum gloeosporioides) e atividade antimicrobiana (Streptococcus pyogenes e Candida parapsilosis). Foram isolados um total de 104 microrganismos endofíticos (fungos filamentosos, bactérias e leveduras), deste universo, 22 fungos filamentosos e 16 bactérias apresentaram inibição de crescimento dos fitopatógenos, e seis bactérias apresentaram atividade antimicrobiana para Streptococcus pyogenes e Candida parapsilosis. Evidenciou-se que os microrganismos endofíticos isolados da planta Acmella ciliata produzem metabólitos com atividade antimicrobiana e antifúngica.Universidade do Estado do AmazonasBrasilPrograma de Pós-Graduação em Biotecnologia e Recursos NaturaisUEAAndrade, Suanni Lemos dehttp://lattes.cnpq.br/7870482573275809Procópio, Rudi Emerson de Limahttp://lattes.cnpq.br/2478199435796976Procópio, Rudi Emerson de Limahttp://lattes.cnpq.br/7870482573275809Ojeda, Cinthya Paola Ortiz2020-03-182020-03-19T00:26:08Z2017-03-182020-03-19T00:26:08Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://repositorioinstitucional.uea.edu.br//handle/riuea/2376porAili T., Fahu P., Siliang H., Gongming Y., Bin L., Tan W. (2014) Characterisation of endophytic Bacillus thuringiensis strains isolated from wheat plants as biocontrol agents against wheat flag smut. Biocontrol Science and Technology, 24 (8) 901-924. Anisha C., Radhakrishnan E.K. (2015) Gliotoxin-producing endophytic Acremonium sp. from Zingiber officinale found antagonistic to soft rot pathogen Pythium myriotylum. Applied Biochemestry and Biotechnology, 175 (7) 3458-3467. Arora S., Vijay S., Kumar D. (2011) Phytochemical and antimicrobial studies on the leaves of Spilanthes acmella. Journal of Chemical and Pharmaceutical Research 3 (5) 145-150. Azevedo J. L. (1998) Microrganismos endofíticos. Em: Melo I. S., Azevedo J. L. (Ed.) Ecologia microbiana. Jaguariúna: EMBRAPA, pp. 117-137. Azevedo J.L., Maccheroni W.J., Pereira J.O., Araújo W.L. (2000) Endophytic microrganisms: a review on insect control and recent advances on tropical plants. Electronic Journal of Biotechnology, 3 (1) 15-16. Badalyan S.M., Innocenti G., Garibyan N.G. (2002) Antagonistic activity of xylotrophic mushrooms against pathogenic fungi of cereals in dual culture. Phytopathologia Mediterranea, Bologna, 41 (3) 200-225. Banhos E.F. dos, Souza A.Q.L. de, Andrade J.C. de, Souza A.D. de, Koolen H.H.F., Albuquerque P.M. (2014) Endophytic fungi from Myrcia guianensis at the Brazilian Amazon: Distribution and bioactivity. Brazilian Journal of Microbiology, 45 (1) 153-161. 59 Bezerra J.D.P., Nascimento C.C.F., Barbosa R.do N., Silva D.C.V. da, Svedese V.M., Silva-Nogueira E., Gomes B.S., Paiva L.M., Souza-Motta C.M. (2015) Endophytic fungi from medicinal plant Bauhinia forficata: Diversity and biotechnological potential. Brazilian Journal of Microbiology, 46 (1) 49-57. Bogas A.C., Ferreira A.J., Araújo W.L., Astolfi-Filho S., Kitajima E.W., Lacava P.T., Azevedo J.L. (2015) Endophytic bacterial diversity in the phyllosphere of Amazon Paullinia cupana associated with asymptomatic and symptomatic anthracnose. Springerplus, 4: 258. Borate P.P., Disale S.D. (2013) Studies on antibacterial activity of Acmella oleracea (L.) Murr. International Journal of Pharmaceutical Science and Health Care, 3 (5) 36-42. 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Journal of Applied Microbiology, 117 (4) 1144-1158.Atribuição-NãoComercial-SemDerivados 3.0 Brasilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade do Estado do Amazonas (UEA)instname:Universidade do Estado do Amazonas (UEA)instacron:UEA2020-03-19T00:26:08Zoai:repositorioinstitucional:riuea/2376Repositório InstitucionalPUBhttp://repositorioinstitucional.uea.edu.br/oai/resquestbibliotecacentral@uea.edu.bropendoar:2023-12-11T16:50:26.128372Repositório Institucional da Universidade do Estado do Amazonas (UEA) - Universidade do Estado do Amazonas (UEA)true |
dc.title.none.fl_str_mv |
Potencial Biotecnológico dos microrganismos endofíticos isolados de plantas de jambu (Acmella ciliata (Kunth) Cass.) para controle de microrganismos patógenos |
title |
Potencial Biotecnológico dos microrganismos endofíticos isolados de plantas de jambu (Acmella ciliata (Kunth) Cass.) para controle de microrganismos patógenos |
spellingShingle |
Potencial Biotecnológico dos microrganismos endofíticos isolados de plantas de jambu (Acmella ciliata (Kunth) Cass.) para controle de microrganismos patógenos Ojeda, Cinthya Paola Ortiz endofítico jambu biotecnologia |
title_short |
Potencial Biotecnológico dos microrganismos endofíticos isolados de plantas de jambu (Acmella ciliata (Kunth) Cass.) para controle de microrganismos patógenos |
title_full |
Potencial Biotecnológico dos microrganismos endofíticos isolados de plantas de jambu (Acmella ciliata (Kunth) Cass.) para controle de microrganismos patógenos |
title_fullStr |
Potencial Biotecnológico dos microrganismos endofíticos isolados de plantas de jambu (Acmella ciliata (Kunth) Cass.) para controle de microrganismos patógenos |
title_full_unstemmed |
Potencial Biotecnológico dos microrganismos endofíticos isolados de plantas de jambu (Acmella ciliata (Kunth) Cass.) para controle de microrganismos patógenos |
title_sort |
Potencial Biotecnológico dos microrganismos endofíticos isolados de plantas de jambu (Acmella ciliata (Kunth) Cass.) para controle de microrganismos patógenos |
author |
Ojeda, Cinthya Paola Ortiz |
author_facet |
Ojeda, Cinthya Paola Ortiz |
author_role |
author |
dc.contributor.none.fl_str_mv |
Andrade, Suanni Lemos de http://lattes.cnpq.br/7870482573275809 Procópio, Rudi Emerson de Lima http://lattes.cnpq.br/2478199435796976 Procópio, Rudi Emerson de Lima http://lattes.cnpq.br/7870482573275809 |
dc.contributor.author.fl_str_mv |
Ojeda, Cinthya Paola Ortiz |
dc.subject.por.fl_str_mv |
endofítico jambu biotecnologia |
topic |
endofítico jambu biotecnologia |
description |
The Amazon rainforest has great biodiversity of species, including animals, plants and microorganisms. Many of these microorganisms are not yet known, as well as some of the potential uses and properties of these. Among these are the endophytic microorganisms who live inside the plants, many of them showing the same properties of the host. The plant “jambu” Acmella ciliata is known for its antimicrobial, antifungal properties among others, and is used in food and natural medicine in the Amazon region. For the isolation of endophytes, first held low-level disinfection of the twigs and leaves of the plant Acmella ciliata, sow and incubate the pieces of this plant material during 15 days at 28ºc. The isolated microorganisms were evaluated in trials of antagonism (Fusarium decemcellulare and Colletotrichum gloeosporioides) and antimicrobial activity (Streptococcus pyogenes and Candida parapsilosis). A total of 104 were isolated endophytic microorganisms (bacteria, filamentous fungi and yeasts), of this universe, 22 filamentous fungi and 16 bacteria showed inhibition of growth of plant pathogens, and six bacteria showed antimicrobial activity for Streptococcus pyogenes and Candida parapsilosis. Was shown that the endophytic microorganisms isolated from plant Acmella ciliata produce metabolites with antimicrobial and antifungal activity. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-03-18 2020-03-18 2020-03-19T00:26:08Z 2020-03-19T00:26:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorioinstitucional.uea.edu.br//handle/riuea/2376 |
url |
http://repositorioinstitucional.uea.edu.br//handle/riuea/2376 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
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Universidade do Estado do Amazonas Brasil Programa de Pós-Graduação em Biotecnologia e Recursos Naturais UEA |
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