Estudo comparativo da microbiota bacteriana cultivável associada à Anopheles darlingi Root, 1926, e seu hábitat

Detalhes bibliográficos
Autor(a) principal: Rocha, Elerson Matos
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da Universidade do Estado do Amazonas (UEA)
Texto Completo: http://repositorioinstitucional.uea.edu.br//handle/riuea/2392
Resumo: Malaria is a disease responsible for thousands of deaths worldwide every year. The etiologic agents are protozoa of the genus Plasmodium that are transmitted by female Anopheles mosquitos, considered vector in these conditions. Despite several measures are employed to control malaria and also of many international control programs being implemented, with the focus always returned to mosquito vectors, which each decade exhibit marked resistance to conventional insecticides, the disease remains very common and It presents thousands of cases, a fact that leads to the search for new vector mosquito control methods. Research based on biological control using micro-organisms, are becoming alternatives for controlling these most frequent form of vectors. Little is known about microbial communities that live in symbiosis with the larvae of Anopheles. Know and understand the functions of the diversity of bacteria that live associated with the malaria vector, will allow to understand how the relationship between bacteria, insects and protozoa happen and extensive research may be used such information for future work in order to control this disease in the world. Molecular methodologies contribute to this development analysis of microbial diversity and can reveal a scenario of the distribution of bacteria in the natural habitat of larval breeding of the malaria vector. This study aimed to make a comparative analysis of cultivable bacterial groups associated with larvae and pupae of Anopheles darlingi and their habitat in the city of Coari and Manaus in Amazonas state. Sampling was carried out in the city of Coari and Manaus. Bacteria were inoculated and purified in selective media. DNA extractions were performed and the identification was carried out from the amplification and sequencing of the 16S rDNA fragment. In this study, 1,845 were isolated bacteria from the city of Coari and Manaus. They were identified from water samples, 3rd and 4th larval and pupal stages of A. darlingi, 46 different species of bacteria belonging to 23 genera phyla Proteobacteria, Actinobacteria, Firmicutes and Bacteroidetes. The species Bacillus sp., Chromobacterium sp., Chromobacterium violaceum, Bacillus thuringiensis and Pseudomonas sp had the highest percentage of isolates obtained. It was obtained a collection that makes the first step towards a promising control study involving malaria bacteria associated with A. darlingi and their habitat in the Brazilian Amazon region. The next step will be to use the isolates identified in this study work aimed at understanding the relationship of bacteria with the malaria parasite in the midgut of the mosquito A. darlingi. Keywords: Bacterial microbiota - Anopheles darlingi - Malaria - Amazon.
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spelling Estudo comparativo da microbiota bacteriana cultivável associada à Anopheles darlingi Root, 1926, e seu hábitatMicrobiota bacterianaAnopheles darlingiMaláriaAmazôniaBiotecnologiaMalaria is a disease responsible for thousands of deaths worldwide every year. The etiologic agents are protozoa of the genus Plasmodium that are transmitted by female Anopheles mosquitos, considered vector in these conditions. Despite several measures are employed to control malaria and also of many international control programs being implemented, with the focus always returned to mosquito vectors, which each decade exhibit marked resistance to conventional insecticides, the disease remains very common and It presents thousands of cases, a fact that leads to the search for new vector mosquito control methods. Research based on biological control using micro-organisms, are becoming alternatives for controlling these most frequent form of vectors. Little is known about microbial communities that live in symbiosis with the larvae of Anopheles. Know and understand the functions of the diversity of bacteria that live associated with the malaria vector, will allow to understand how the relationship between bacteria, insects and protozoa happen and extensive research may be used such information for future work in order to control this disease in the world. Molecular methodologies contribute to this development analysis of microbial diversity and can reveal a scenario of the distribution of bacteria in the natural habitat of larval breeding of the malaria vector. This study aimed to make a comparative analysis of cultivable bacterial groups associated with larvae and pupae of Anopheles darlingi and their habitat in the city of Coari and Manaus in Amazonas state. Sampling was carried out in the city of Coari and Manaus. Bacteria were inoculated and purified in selective media. DNA extractions were performed and the identification was carried out from the amplification and sequencing of the 16S rDNA fragment. In this study, 1,845 were isolated bacteria from the city of Coari and Manaus. They were identified from water samples, 3rd and 4th larval and pupal stages of A. darlingi, 46 different species of bacteria belonging to 23 genera phyla Proteobacteria, Actinobacteria, Firmicutes and Bacteroidetes. The species Bacillus sp., Chromobacterium sp., Chromobacterium violaceum, Bacillus thuringiensis and Pseudomonas sp had the highest percentage of isolates obtained. It was obtained a collection that makes the first step towards a promising control study involving malaria bacteria associated with A. darlingi and their habitat in the Brazilian Amazon region. The next step will be to use the isolates identified in this study work aimed at understanding the relationship of bacteria with the malaria parasite in the midgut of the mosquito A. darlingi. Keywords: Bacterial microbiota - Anopheles darlingi - Malaria - Amazon.A malária é uma doença responsável por milhares de mortes no mundo a cada ano. Os agentes etiológicos são protozoários do gênero Plasmodium que são transmitidos pela fêmea de mosquitos do gênero Anopheles, considerada vetor nessas condições. Apesar de várias medidas serem empregadas para o controle da malária e também de diversos programas internacionais de controle sendo implantados, com o foco sempre voltado aos mosquitos vetores, que a cada década apresentam acentuadas resistências aos inseticidas convencionais, a doença continua a ser muito frequente e apresenta milhares de casos, fato este que acarreta na busca de novos métodos de controle do mosquito vetor. Pesquisas com base em controle biológico, utilizando micro-organismos, vêm se tornando alternativas para o controle desses vetores de forma mais frequente. Pouco se conhece a respeito de comunidades microbianas que convivem em simbiose com as larvas de anofelinos. Conhecer e entender as funções da diversidade de bactérias que vivem associadas ao vetor da malária, possibilitará entender como as relações entre bactérias, insetos e protozoário acontecem e inúmeras pesquisas poderão se utilizar dessas informações para futuros trabalhos com a finalidade de controlar esta doença no mundo. Metodologias moleculares contribuem para esse desenvolvimento de análises da diversidade microbiana e podem revelar um cenário da distribuição de bactérias no hábitat natural de procriação larval do vetor da malária. Este estudo teve por objetivo fazer uma análise comparativa de grupos bacterianos cultiváveis associados a larvas e pupa de Anopheles darlingi e seu hábitat no Município de Coari e Manaus no Estado do Amazonas. Foram realizadas coletas no município de Coari e Manaus. As bactérias foram inoculadas e purificadas em meios seletivos. Foram realizadas extrações de DNA e a identificação foi realizada a partir da amplificação e sequenciamento do fragmento do rDNA 16S. Neste estudo, foram isoladas 1.845 bactérias do Município de Coari e Manaus. Foram identificadas entre as amostras de água, larva de 3° e 4° estádio e pupa de A. darlingi, 46 diferentes espécies de bactérias de 23 gêneros pertencentes aos filos Proteobacteria, Actinobacteria, Firmicutes, e Bacteroidetes. As espécies Bacillus sp., Chromobacterium sp., Chromobacterium violaceum, Bacillus thuringiensis e Pseudomonas sp apresentaram a maior porcentagem dos isolados obtidos. Foi obtida uma coleção que se torna o passo inicial para um estudo promissor de controle da malária envolvendo bactérias associadas a A. darlingi e seu hábitat na Região Amazônica Brasileira. O próximo passo será utilizar os isolados identificados neste estudo em trabalhos que visam compreender a relação das bactérias com o parasita da malária no intestino médio do mosquito A. darlingi. Palavras-chave: Microbiota bacteriana - Anopheles darlingi – malária – Amazônia.Universidade do Estado do AmazonasBrasilPrograma de Pós-Graduação em Biotecnologia e Recursos Naturais da AmazôniaUEASouza, Antonia Queiroz Lima dehttp://lattes.cnpq.br/8499987875894209Tadei, Wanderli Pedrohttp://lattes.cnpq.br/6806722604010480Tadei, Wanderli Pedrohttp://lattes.cnpq.br/6806722604010480Silva , Ademir Castro ehttp://lattes.cnpq.br/5162043375426666Pessoa, Marcos Cézar Fernandeshttp://lattes.cnpq.br/6237137010678999Rocha, Elerson Matos2020-03-202020-03-19T01:13:47Z2015-05-292020-03-19T01:13:47Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://repositorioinstitucional.uea.edu.br//handle/riuea/2392porABALLAY, A. & AUSUBEL, F.M. Caenorhabditis elegans as a host the study of host-pathogen interaction. Curr. Opin. Microbiol. 5, 97-101. 2002. AGOGUE H, CASAMAYOR EO, JOUX F, OBERNOSTERER I, DUPUY C, LANTOINE F et al. (2004). 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Dissertação (mestrado)- INPA.Atribuição-NãoComercial-SemDerivados 3.0 Brasilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade do Estado do Amazonas (UEA)instname:Universidade do Estado do Amazonas (UEA)instacron:UEA2020-03-19T01:13:47Zoai:repositorioinstitucional:riuea/2392Repositório InstitucionalPUBhttp://repositorioinstitucional.uea.edu.br/oai/resquestbibliotecacentral@uea.edu.bropendoar:2023-12-11T16:50:36.154262Repositório Institucional da Universidade do Estado do Amazonas (UEA) - Universidade do Estado do Amazonas (UEA)true
dc.title.none.fl_str_mv Estudo comparativo da microbiota bacteriana cultivável associada à Anopheles darlingi Root, 1926, e seu hábitat
title Estudo comparativo da microbiota bacteriana cultivável associada à Anopheles darlingi Root, 1926, e seu hábitat
spellingShingle Estudo comparativo da microbiota bacteriana cultivável associada à Anopheles darlingi Root, 1926, e seu hábitat
Rocha, Elerson Matos
Microbiota bacteriana
Anopheles darlingi
Malária
Amazônia
Biotecnologia
title_short Estudo comparativo da microbiota bacteriana cultivável associada à Anopheles darlingi Root, 1926, e seu hábitat
title_full Estudo comparativo da microbiota bacteriana cultivável associada à Anopheles darlingi Root, 1926, e seu hábitat
title_fullStr Estudo comparativo da microbiota bacteriana cultivável associada à Anopheles darlingi Root, 1926, e seu hábitat
title_full_unstemmed Estudo comparativo da microbiota bacteriana cultivável associada à Anopheles darlingi Root, 1926, e seu hábitat
title_sort Estudo comparativo da microbiota bacteriana cultivável associada à Anopheles darlingi Root, 1926, e seu hábitat
author Rocha, Elerson Matos
author_facet Rocha, Elerson Matos
author_role author
dc.contributor.none.fl_str_mv Souza, Antonia Queiroz Lima de
http://lattes.cnpq.br/8499987875894209
Tadei, Wanderli Pedro
http://lattes.cnpq.br/6806722604010480
Tadei, Wanderli Pedro
http://lattes.cnpq.br/6806722604010480
Silva , Ademir Castro e
http://lattes.cnpq.br/5162043375426666
Pessoa, Marcos Cézar Fernandes
http://lattes.cnpq.br/6237137010678999
dc.contributor.author.fl_str_mv Rocha, Elerson Matos
dc.subject.por.fl_str_mv Microbiota bacteriana
Anopheles darlingi
Malária
Amazônia
Biotecnologia
topic Microbiota bacteriana
Anopheles darlingi
Malária
Amazônia
Biotecnologia
description Malaria is a disease responsible for thousands of deaths worldwide every year. The etiologic agents are protozoa of the genus Plasmodium that are transmitted by female Anopheles mosquitos, considered vector in these conditions. Despite several measures are employed to control malaria and also of many international control programs being implemented, with the focus always returned to mosquito vectors, which each decade exhibit marked resistance to conventional insecticides, the disease remains very common and It presents thousands of cases, a fact that leads to the search for new vector mosquito control methods. Research based on biological control using micro-organisms, are becoming alternatives for controlling these most frequent form of vectors. Little is known about microbial communities that live in symbiosis with the larvae of Anopheles. Know and understand the functions of the diversity of bacteria that live associated with the malaria vector, will allow to understand how the relationship between bacteria, insects and protozoa happen and extensive research may be used such information for future work in order to control this disease in the world. Molecular methodologies contribute to this development analysis of microbial diversity and can reveal a scenario of the distribution of bacteria in the natural habitat of larval breeding of the malaria vector. This study aimed to make a comparative analysis of cultivable bacterial groups associated with larvae and pupae of Anopheles darlingi and their habitat in the city of Coari and Manaus in Amazonas state. Sampling was carried out in the city of Coari and Manaus. Bacteria were inoculated and purified in selective media. DNA extractions were performed and the identification was carried out from the amplification and sequencing of the 16S rDNA fragment. In this study, 1,845 were isolated bacteria from the city of Coari and Manaus. They were identified from water samples, 3rd and 4th larval and pupal stages of A. darlingi, 46 different species of bacteria belonging to 23 genera phyla Proteobacteria, Actinobacteria, Firmicutes and Bacteroidetes. The species Bacillus sp., Chromobacterium sp., Chromobacterium violaceum, Bacillus thuringiensis and Pseudomonas sp had the highest percentage of isolates obtained. It was obtained a collection that makes the first step towards a promising control study involving malaria bacteria associated with A. darlingi and their habitat in the Brazilian Amazon region. The next step will be to use the isolates identified in this study work aimed at understanding the relationship of bacteria with the malaria parasite in the midgut of the mosquito A. darlingi. Keywords: Bacterial microbiota - Anopheles darlingi - Malaria - Amazon.
publishDate 2015
dc.date.none.fl_str_mv 2015-05-29
2020-03-20
2020-03-19T01:13:47Z
2020-03-19T01:13:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorioinstitucional.uea.edu.br//handle/riuea/2392
url http://repositorioinstitucional.uea.edu.br//handle/riuea/2392
dc.language.iso.fl_str_mv por
language por
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dc.rights.driver.fl_str_mv Atribuição-NãoComercial-SemDerivados 3.0 Brasil
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dc.publisher.none.fl_str_mv Universidade do Estado do Amazonas
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Programa de Pós-Graduação em Biotecnologia e Recursos Naturais da Amazônia
UEA
publisher.none.fl_str_mv Universidade do Estado do Amazonas
Brasil
Programa de Pós-Graduação em Biotecnologia e Recursos Naturais da Amazônia
UEA
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repository.name.fl_str_mv Repositório Institucional da Universidade do Estado do Amazonas (UEA) - Universidade do Estado do Amazonas (UEA)
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