Cultura de embrião zigótico, calogênise e conservação in vitro de Erythrina velutina Willd. (Leguminosae)

Detalhes bibliográficos
Autor(a) principal: Fonseca, Priscila Tavares
Data de Publicação: 2012
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UEFS
Texto Completo: http://tede2.uefs.br:8080/handle/tede/1047
Resumo: Erythrina velutina is a native Caatinga biome, with increasing socio-economic importance and medical. The objective of this work were to culture zygotic embryo, as well as in vitro E. velutina callus formation and conservation. The first experiment was a comparative study of seed germination between intact and embryo rescue evaluating the effect of plant growth regulators BAP and NAA on in vitro morphogenesis. The second experiment was verify the effect of BAP and CIN in the nodal segments regeneration. The third experiment observed the effect of 2,4-D on in vitro callus formation from different explants: zygotic embryos whole and sectioned, leaves and epicotyl. In callus cultures of zygotic embryos we analyzed the kinetics growth, quantified if the AR and AST did to plant anatomy. In the last experiment evaluated the effect of sucrose osmotic agent and the PBZ growth retardant the in vitro conservation. For germination of zygotic embryos cultivation provided more vigorous plants. The plumule explant and zygotic embryos can be regenerated at concentration of 4.0μM of BAP, the intermediate regions and the radicle promoted formation of compact callus on the combination of 10.63μM BAP and 2.0μM NAA. In regeneration in vitro concentration of 21.03μM BAP induced higher number of shoots in nodal segments. For callus formation concentration of 10.65μM 2,4-D induced friable callus on zygotic embryos whole and the reduction of AR, AST and the presence of dividing cells and amyloplasts during the growing period. The growth curve showed that callus should be transferred to subculture medium between the 15th to the 21st day. In the tested concentration of 11.75μM 2,4-D was obtained friable callus on leaf segments. We observed slower cultures growth at a temperature of 18°C, concentrations of up to 175.28mM sucrose was not sufficient to reduce plant growth. When using PBZ was observed a reduction of plant growth when added to the culture medium 4μM of BPZ.
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spelling Santana, José Raniere Ferreira de01664354573http://lattes.cnpq.br/3884092334137076Fonseca, Priscila Tavares2020-04-23T00:02:03Z2012-02-27FONSECA, Priscila Tavares. Cultura de embrião zigótico, calogênise e conservação in vitro de Erythrina velutina Willd. (Leguminosae). 2012. 92 f. Dissertação (Mestrado Acadêmico em Recursos Genéticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2012.http://tede2.uefs.br:8080/handle/tede/1047Erythrina velutina is a native Caatinga biome, with increasing socio-economic importance and medical. The objective of this work were to culture zygotic embryo, as well as in vitro E. velutina callus formation and conservation. The first experiment was a comparative study of seed germination between intact and embryo rescue evaluating the effect of plant growth regulators BAP and NAA on in vitro morphogenesis. The second experiment was verify the effect of BAP and CIN in the nodal segments regeneration. The third experiment observed the effect of 2,4-D on in vitro callus formation from different explants: zygotic embryos whole and sectioned, leaves and epicotyl. In callus cultures of zygotic embryos we analyzed the kinetics growth, quantified if the AR and AST did to plant anatomy. In the last experiment evaluated the effect of sucrose osmotic agent and the PBZ growth retardant the in vitro conservation. For germination of zygotic embryos cultivation provided more vigorous plants. The plumule explant and zygotic embryos can be regenerated at concentration of 4.0μM of BAP, the intermediate regions and the radicle promoted formation of compact callus on the combination of 10.63μM BAP and 2.0μM NAA. In regeneration in vitro concentration of 21.03μM BAP induced higher number of shoots in nodal segments. For callus formation concentration of 10.65μM 2,4-D induced friable callus on zygotic embryos whole and the reduction of AR, AST and the presence of dividing cells and amyloplasts during the growing period. The growth curve showed that callus should be transferred to subculture medium between the 15th to the 21st day. In the tested concentration of 11.75μM 2,4-D was obtained friable callus on leaf segments. We observed slower cultures growth at a temperature of 18°C, concentrations of up to 175.28mM sucrose was not sufficient to reduce plant growth. When using PBZ was observed a reduction of plant growth when added to the culture medium 4μM of BPZ.Erythrina velutina é nativa do bioma Caatinga, apresentando relevada importância sócio-econômica e medicinal. Objetivou-se nesse trabalho o cultivo de embrião zigótico, bem como, calogênese e conservação in vitro de E. velutina. No primeiro experimento foi feito estudo comparativo da germinação entre sementes intactas e o cultivo de embrião avaliando o efeito dos reguladores vegetais BAP e ANA sobre a morfogênese in vitro. No segundo experimento verificou-se o efeito de BAP e CIN na regeneração de segmentos nodais. No terceiro experimento observou-se o efeito do 2,4-D na calogênese in vitro de diferentes explantes: embriões zigóticos inteiro e seccionado, folhas e epicótilo. Nos calos de embriões zigóticos analisou-se o crescimento cinético, quantificou-se os AR e AST e fez-se a anatomia vegetal. No último experimento avaliou-se o efeito do agente osmótico sacarose e do retardante de crescimento PBZ na conservação in vitro. Para germinação o cultivo de embriões zigóticos proporcionou plantas mais vigorosas. O explante plúmula e embriões zigóticos podem ser regenerados na concentração de 4,0µM de BAP, as regiões intermediária e radícula promoveram a formação de calos compactos na combinação de 10,63µM BAP e 2,0µM de ANA. Na regeneração in vitro a concentração de 20µM de BAP induziu maior número de brotos em segmentos nodais. Para calogênese a concentração de 11,75µM de 2,4-D induziu calos friáveis em embriões zigóticos inteiros sendo verificado a redução dos AR, AST e presença de células em divisão e amiloplastos durante o período de cultivo. A curva de crescimento mostrou que os calos devem ser transferidos para meio de subcultivo entre o 15o até o 21o dia. Na concentração testada de 11,75µM de 2,4-D obteve-se calos friáveis de segmentos foliares. Foi observado crescimento mais lento das culturas na temperatura de 18ºC, a concentração de sacarose até 175,28mM não foram suficientes para reduzir o crescimento das plantas. Quando acrescido ao meio de cultura de 4µM de BPZ nota-se redução no crescimento das plantas.Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2020-04-23T00:02:03Z No. of bitstreams: 1 Dissertação_Priscila Tavares.pdf: 3165609 bytes, checksum: 7c319f735dd32f4470834727b79fddd3 (MD5)Made available in DSpace on 2020-04-23T00:02:03Z (GMT). No. of bitstreams: 1 Dissertação_Priscila Tavares.pdf: 3165609 bytes, checksum: 7c319f735dd32f4470834727b79fddd3 (MD5) Previous issue date: 2012-02-27Fundação de Amparo à Pesquisa do Estado da Bahia - FAPEBapplication/pdfporUniversidade Estadual de Feira de SantanaMestrado Acadêmico em Recursos Genéticos VegetaisUEFSBrasilDEPARTAMENTO DE CIÊNCIAS BIOLÓGICASMulunguRegeneração in vitroCitocininasAuxinasCalogêneseCrescimento mínimoIn vitro regenerationCytokininAuxinCallus formationMinimum growthCIENCIAS BIOLOGICASCIENCIAS BIOLOGICAS::BIOLOGIA GERALCultura de embrião zigótico, calogênise e conservação in vitro de Erythrina velutina Willd. 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dc.title.por.fl_str_mv Cultura de embrião zigótico, calogênise e conservação in vitro de Erythrina velutina Willd. (Leguminosae)
title Cultura de embrião zigótico, calogênise e conservação in vitro de Erythrina velutina Willd. (Leguminosae)
spellingShingle Cultura de embrião zigótico, calogênise e conservação in vitro de Erythrina velutina Willd. (Leguminosae)
Fonseca, Priscila Tavares
Mulungu
Regeneração in vitro
Citocininas
Auxinas
Calogênese
Crescimento mínimo
In vitro regeneration
Cytokinin
Auxin
Callus formation
Minimum growth
CIENCIAS BIOLOGICAS
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
title_short Cultura de embrião zigótico, calogênise e conservação in vitro de Erythrina velutina Willd. (Leguminosae)
title_full Cultura de embrião zigótico, calogênise e conservação in vitro de Erythrina velutina Willd. (Leguminosae)
title_fullStr Cultura de embrião zigótico, calogênise e conservação in vitro de Erythrina velutina Willd. (Leguminosae)
title_full_unstemmed Cultura de embrião zigótico, calogênise e conservação in vitro de Erythrina velutina Willd. (Leguminosae)
title_sort Cultura de embrião zigótico, calogênise e conservação in vitro de Erythrina velutina Willd. (Leguminosae)
author Fonseca, Priscila Tavares
author_facet Fonseca, Priscila Tavares
author_role author
dc.contributor.advisor1.fl_str_mv Santana, José Raniere Ferreira de
dc.contributor.authorID.fl_str_mv 01664354573
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/3884092334137076
dc.contributor.author.fl_str_mv Fonseca, Priscila Tavares
contributor_str_mv Santana, José Raniere Ferreira de
dc.subject.por.fl_str_mv Mulungu
Regeneração in vitro
Citocininas
Auxinas
Calogênese
Crescimento mínimo
topic Mulungu
Regeneração in vitro
Citocininas
Auxinas
Calogênese
Crescimento mínimo
In vitro regeneration
Cytokinin
Auxin
Callus formation
Minimum growth
CIENCIAS BIOLOGICAS
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
dc.subject.eng.fl_str_mv In vitro regeneration
Cytokinin
Auxin
Callus formation
Minimum growth
dc.subject.cnpq.fl_str_mv CIENCIAS BIOLOGICAS
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
description Erythrina velutina is a native Caatinga biome, with increasing socio-economic importance and medical. The objective of this work were to culture zygotic embryo, as well as in vitro E. velutina callus formation and conservation. The first experiment was a comparative study of seed germination between intact and embryo rescue evaluating the effect of plant growth regulators BAP and NAA on in vitro morphogenesis. The second experiment was verify the effect of BAP and CIN in the nodal segments regeneration. The third experiment observed the effect of 2,4-D on in vitro callus formation from different explants: zygotic embryos whole and sectioned, leaves and epicotyl. In callus cultures of zygotic embryos we analyzed the kinetics growth, quantified if the AR and AST did to plant anatomy. In the last experiment evaluated the effect of sucrose osmotic agent and the PBZ growth retardant the in vitro conservation. For germination of zygotic embryos cultivation provided more vigorous plants. The plumule explant and zygotic embryos can be regenerated at concentration of 4.0μM of BAP, the intermediate regions and the radicle promoted formation of compact callus on the combination of 10.63μM BAP and 2.0μM NAA. In regeneration in vitro concentration of 21.03μM BAP induced higher number of shoots in nodal segments. For callus formation concentration of 10.65μM 2,4-D induced friable callus on zygotic embryos whole and the reduction of AR, AST and the presence of dividing cells and amyloplasts during the growing period. The growth curve showed that callus should be transferred to subculture medium between the 15th to the 21st day. In the tested concentration of 11.75μM 2,4-D was obtained friable callus on leaf segments. We observed slower cultures growth at a temperature of 18°C, concentrations of up to 175.28mM sucrose was not sufficient to reduce plant growth. When using PBZ was observed a reduction of plant growth when added to the culture medium 4μM of BPZ.
publishDate 2012
dc.date.issued.fl_str_mv 2012-02-27
dc.date.accessioned.fl_str_mv 2020-04-23T00:02:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.citation.fl_str_mv FONSECA, Priscila Tavares. Cultura de embrião zigótico, calogênise e conservação in vitro de Erythrina velutina Willd. (Leguminosae). 2012. 92 f. Dissertação (Mestrado Acadêmico em Recursos Genéticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2012.
dc.identifier.uri.fl_str_mv http://tede2.uefs.br:8080/handle/tede/1047
identifier_str_mv FONSECA, Priscila Tavares. Cultura de embrião zigótico, calogênise e conservação in vitro de Erythrina velutina Willd. (Leguminosae). 2012. 92 f. Dissertação (Mestrado Acadêmico em Recursos Genéticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2012.
url http://tede2.uefs.br:8080/handle/tede/1047
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