Identificação e mapeamento de marcadores associados a locos de resistência a virose do mosaico dourado em feijão caupi (Vignaunguiculata (L.) Walp.)

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Marciene Amorim
Data de Publicação: 2011
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UEFS
Texto Completo: http://tede2.uefs.br:8080/handle/tede/1074
Resumo: The objective of this work was the identification of markers linked to resistant genes to the cowpea golden mosaic virus (CGMV) through AFLP markers and the development and validation of SCAR markers linked to the resistance genes. For the study, the F2 population (286 individuals) resulting from the cross between IT97K-499-35, resistant genitor, and Canapu T16, susceptible, was used. The methodologies of Bulked Segregant Analysis and AFLP were used to identify markers linked to the resistance gene. The program JoinMap version 2.0 was used for construction of the map, adopting a minimum LOD of 3.0, a maximum recombination frequency of 0.45, and the Kosambi mapping function. Among the 196 combinations of AFLP primers tested, three markers linked to the resistance gene to CGMV were identified. The markers presented distances of 4.3 cM (E.AAC/M.CCC515), 14.2 cM (E.AGG/M.CTT280), and 16.8 cM (E.AAA/M.CAG352) of the resistance gene to golden mosaic, with the first two flanking it. The LOD scores were 50.4, 24.4, and 28.7, respectively. Eight SCAR markers were developed from the AFLP markers E.AAC/M.CCC515 and E.AAA/M.CAG352. In the eight combinations of SCAR primers tested among resistant and susceptible individuals of the F2 population, it was not possible to obtain the same segregation observed in the AFLP analysis. Efforts to develop SCAR markers efficient in detecting the resistance gene from AFLP markers obtained in the mapping of the gene that confers resistance to CGMV continue being done.
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spelling Santana, José Raniere Ferreira de5515544854904405135452http://lattes.cnpq.br/6547881854777229Rodrigues, Marciene Amorim2020-05-03T22:46:06Z2011-03-14RODRIGUES, Marciene Amorim. Identificação e mapeamento de marcadores associados a locos de resistência a virose do mosaico dourado em feijão caupi (Vignaunguiculata (L.) Walp.). 2011. 60 f. Dissertação (Mestrado Acadêmico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2011.http://tede2.uefs.br:8080/handle/tede/1074The objective of this work was the identification of markers linked to resistant genes to the cowpea golden mosaic virus (CGMV) through AFLP markers and the development and validation of SCAR markers linked to the resistance genes. For the study, the F2 population (286 individuals) resulting from the cross between IT97K-499-35, resistant genitor, and Canapu T16, susceptible, was used. The methodologies of Bulked Segregant Analysis and AFLP were used to identify markers linked to the resistance gene. The program JoinMap version 2.0 was used for construction of the map, adopting a minimum LOD of 3.0, a maximum recombination frequency of 0.45, and the Kosambi mapping function. Among the 196 combinations of AFLP primers tested, three markers linked to the resistance gene to CGMV were identified. The markers presented distances of 4.3 cM (E.AAC/M.CCC515), 14.2 cM (E.AGG/M.CTT280), and 16.8 cM (E.AAA/M.CAG352) of the resistance gene to golden mosaic, with the first two flanking it. The LOD scores were 50.4, 24.4, and 28.7, respectively. Eight SCAR markers were developed from the AFLP markers E.AAC/M.CCC515 and E.AAA/M.CAG352. In the eight combinations of SCAR primers tested among resistant and susceptible individuals of the F2 population, it was not possible to obtain the same segregation observed in the AFLP analysis. Efforts to develop SCAR markers efficient in detecting the resistance gene from AFLP markers obtained in the mapping of the gene that confers resistance to CGMV continue being done.O objetivo desse trabalho foi a identificação de marcadores associados aos genes de resistência ao vírus do mosaico dourado do feijão caupi (CGMV) por meio do mapeamento de marcadores AFLP e o desenvolvimento e validação de marcadores SCAR ligados aos genes de resistência. Para o estudo, foi utilizada a população de 286 indivíduos F2 resultante do cruzamento entre IT97K-499-35, genitor resistente, e Canapu T16, suscetível. Foram utilizadas as metodologias de ‘Bulked segregant analysis’ e AFLP para identificar marcadores ligados ao gene de resistência. O programa JoinMap versão 2.0 foi usado para construção do mapa, adotando-se LOD mínimo de 3,0, frequência máxima de recombinação de 0,45, e função de mapeamento Kosambi. Dentre as 196 combinações de primers AFLP testadas, foram identificados três marcadores ligados ao gene de resistência ao CGMV. Os marcadores apresentaram distâncias de 4,3 cM (E.AAC/M.CCC515), 14,2 cM (E.AGG/M.CTT280) e 16,8 cM (E.AAA/M.CAG352) do gene de resistência ao mosaico dourado, com os dois primeiros flanqueando-o. Os LODs foram 50,4, 24,4 e 28,7, respectivamente. Foram desenvolvidos oito marcadores SCARs a partir das marcas AFLP E.AAC/M.CCC515 e E.AAA/M.CAG352. Nas oito combinações de primers SCARs testados entre indivíduos resistentes e suscetíveis da população F2, não foi possível obter a mesma segregação observada na análise AFLP. Esforços para desenvolver marcadores SCARs eficientes em detectar o gene de resistência a partir das marcas AFLP obtidas no mapeamento do gene que confere resistência ao CGMV continuam sendo realizados.Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2020-05-03T22:46:06Z No. of bitstreams: 1 Disseratação_Marciene Amorim Rodrigues.[1].pdf: 1219975 bytes, checksum: eee2f455c35fb9e878e2d0a6432a26fe (MD5)Made available in DSpace on 2020-05-03T22:46:06Z (GMT). No. of bitstreams: 1 Disseratação_Marciene Amorim Rodrigues.[1].pdf: 1219975 bytes, checksum: eee2f455c35fb9e878e2d0a6432a26fe (MD5) Previous issue date: 2011-03-14Fundação de Amparo à Pesquisa do Estado da Bahia - FAPEBapplication/pdfporUniversidade Estadual de Feira de SantanaMestrado Acadêmico em BiotecnologiaUEFSBrasilDEPARTAMENTO DE CIÊNCIAS BIOLÓGICASMapeamento genéticoMarcadores molecularesCowpea golden mosaic virusVigna unguiculataGenetic mappingMolecular markersCIENCIAS BIOLOGICAS::BIOLOGIA GERALCIENCIAS BIOLOGICASIdentificação e mapeamento de marcadores associados a locos de resistência a virose do mosaico dourado em feijão caupi (Vignaunguiculata (L.) 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dc.title.por.fl_str_mv Identificação e mapeamento de marcadores associados a locos de resistência a virose do mosaico dourado em feijão caupi (Vignaunguiculata (L.) Walp.)
title Identificação e mapeamento de marcadores associados a locos de resistência a virose do mosaico dourado em feijão caupi (Vignaunguiculata (L.) Walp.)
spellingShingle Identificação e mapeamento de marcadores associados a locos de resistência a virose do mosaico dourado em feijão caupi (Vignaunguiculata (L.) Walp.)
Rodrigues, Marciene Amorim
Mapeamento genético
Marcadores moleculares
Cowpea golden mosaic virus
Vigna unguiculata
Genetic mapping
Molecular markers
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
CIENCIAS BIOLOGICAS
title_short Identificação e mapeamento de marcadores associados a locos de resistência a virose do mosaico dourado em feijão caupi (Vignaunguiculata (L.) Walp.)
title_full Identificação e mapeamento de marcadores associados a locos de resistência a virose do mosaico dourado em feijão caupi (Vignaunguiculata (L.) Walp.)
title_fullStr Identificação e mapeamento de marcadores associados a locos de resistência a virose do mosaico dourado em feijão caupi (Vignaunguiculata (L.) Walp.)
title_full_unstemmed Identificação e mapeamento de marcadores associados a locos de resistência a virose do mosaico dourado em feijão caupi (Vignaunguiculata (L.) Walp.)
title_sort Identificação e mapeamento de marcadores associados a locos de resistência a virose do mosaico dourado em feijão caupi (Vignaunguiculata (L.) Walp.)
author Rodrigues, Marciene Amorim
author_facet Rodrigues, Marciene Amorim
author_role author
dc.contributor.advisor1.fl_str_mv Santana, José Raniere Ferreira de
dc.contributor.advisor1ID.fl_str_mv 55155448549
dc.contributor.authorID.fl_str_mv 04405135452
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/6547881854777229
dc.contributor.author.fl_str_mv Rodrigues, Marciene Amorim
contributor_str_mv Santana, José Raniere Ferreira de
dc.subject.por.fl_str_mv Mapeamento genético
Marcadores moleculares
Cowpea golden mosaic virus
Vigna unguiculata
topic Mapeamento genético
Marcadores moleculares
Cowpea golden mosaic virus
Vigna unguiculata
Genetic mapping
Molecular markers
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
CIENCIAS BIOLOGICAS
dc.subject.eng.fl_str_mv Genetic mapping
Molecular markers
dc.subject.cnpq.fl_str_mv CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
CIENCIAS BIOLOGICAS
description The objective of this work was the identification of markers linked to resistant genes to the cowpea golden mosaic virus (CGMV) through AFLP markers and the development and validation of SCAR markers linked to the resistance genes. For the study, the F2 population (286 individuals) resulting from the cross between IT97K-499-35, resistant genitor, and Canapu T16, susceptible, was used. The methodologies of Bulked Segregant Analysis and AFLP were used to identify markers linked to the resistance gene. The program JoinMap version 2.0 was used for construction of the map, adopting a minimum LOD of 3.0, a maximum recombination frequency of 0.45, and the Kosambi mapping function. Among the 196 combinations of AFLP primers tested, three markers linked to the resistance gene to CGMV were identified. The markers presented distances of 4.3 cM (E.AAC/M.CCC515), 14.2 cM (E.AGG/M.CTT280), and 16.8 cM (E.AAA/M.CAG352) of the resistance gene to golden mosaic, with the first two flanking it. The LOD scores were 50.4, 24.4, and 28.7, respectively. Eight SCAR markers were developed from the AFLP markers E.AAC/M.CCC515 and E.AAA/M.CAG352. In the eight combinations of SCAR primers tested among resistant and susceptible individuals of the F2 population, it was not possible to obtain the same segregation observed in the AFLP analysis. Efforts to develop SCAR markers efficient in detecting the resistance gene from AFLP markers obtained in the mapping of the gene that confers resistance to CGMV continue being done.
publishDate 2011
dc.date.issued.fl_str_mv 2011-03-14
dc.date.accessioned.fl_str_mv 2020-05-03T22:46:06Z
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dc.identifier.citation.fl_str_mv RODRIGUES, Marciene Amorim. Identificação e mapeamento de marcadores associados a locos de resistência a virose do mosaico dourado em feijão caupi (Vignaunguiculata (L.) Walp.). 2011. 60 f. Dissertação (Mestrado Acadêmico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2011.
dc.identifier.uri.fl_str_mv http://tede2.uefs.br:8080/handle/tede/1074
identifier_str_mv RODRIGUES, Marciene Amorim. Identificação e mapeamento de marcadores associados a locos de resistência a virose do mosaico dourado em feijão caupi (Vignaunguiculata (L.) Walp.). 2011. 60 f. Dissertação (Mestrado Acadêmico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2011.
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