Micropropagação e análises bioquímicas e fitoquímicas de Physalis peruviana L.
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UEFS |
Texto Completo: | http://tede2.uefs.br:8080/handle/tede/754 |
Resumo: | Physalis peruviana L. (Solanaceae) is a fruit herbaceous, which has been consolidating in the small fruit market due to its nutritional, medicinal potentialities and relevant concentration of vitamins, minerals and secondary metabolites of interest to the pharmaceutical industry. The barriers to its production are the short reproductive cycle, the involvement of the fruits by pests and the lack of studies for the species. Micropropagation is an alternative for the propagation of medicinal species, since this technique allows the production of a large number of seedlings with phytosanitary quality, besides being a tool for genetic improvement and for phytochemical studies. This study aims to evaluate the in vitro morphogenesis of P. peruviana and to analyze the biochemical and phytochemical profile of the species in different cultures. Cotyledon node, cotyledon, leaf, epicotyl, hypocotyl and root explants were inoculated in half-saline MS culture medium supplemented with BAP (0,00; 2,22; 4,44; 6,66 and 8,88µM), plus 30 gL-1 of sucrose and 7 gL-1 of agar; and the explant cotyledonary node was submitted to (0,00; 2,22; 4,44; 6,66; 8,88; 13,32; 17,76 and 22,20 μM), plus 30 gL-1 of sucrose and 7 gL-1 of agar. It was verified that the cotyledon node submitted to the concentration of 12,50 μM BAP is the best source of explant, with regeneration of the shoots via direct organogenesis, and the leaf is the most efficient explant for the indirect organogenesis in concentrations of 4,44 μM BAP. Histological analysis proved the pathways of regeneration. The shoots from the direct organogenesis were rooted in vitro in MS½ medium free of plant regulator, and the microplants acclimatized in vegetal soil, reached 100% survival at 90 days of acclimatization. Biochemical and phytochemical analyzes of stem, root and leaf explants were carried out in vitro and ex vitro. The analysis showed that in vitro cultivation favors the synthesis of proteins and reducer sugars in leaf and root tissues. The metabolite rutin is produced in leaf explants in greenhouse. |
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Brito, Alone Lima5219040456883508309568http://lattes.cnpq.br/7398279864316232Mascarenhas, Lilian Marcia Santana2019-05-07T21:16:49Z2018-06-05MASCARENHAS, Lilian Marcia Santana. Micropropagação e análises bioquímicas e fitoquímicas de Physalis peruviana L. 2018. 75 f. Dissertação (Mestrado Acadêmico em Recursos Genéticos Vegetais) - Universidade Estadual de Feira de Santana, Feira de Santana, 2018.http://tede2.uefs.br:8080/handle/tede/754Physalis peruviana L. (Solanaceae) is a fruit herbaceous, which has been consolidating in the small fruit market due to its nutritional, medicinal potentialities and relevant concentration of vitamins, minerals and secondary metabolites of interest to the pharmaceutical industry. The barriers to its production are the short reproductive cycle, the involvement of the fruits by pests and the lack of studies for the species. Micropropagation is an alternative for the propagation of medicinal species, since this technique allows the production of a large number of seedlings with phytosanitary quality, besides being a tool for genetic improvement and for phytochemical studies. This study aims to evaluate the in vitro morphogenesis of P. peruviana and to analyze the biochemical and phytochemical profile of the species in different cultures. Cotyledon node, cotyledon, leaf, epicotyl, hypocotyl and root explants were inoculated in half-saline MS culture medium supplemented with BAP (0,00; 2,22; 4,44; 6,66 and 8,88µM), plus 30 gL-1 of sucrose and 7 gL-1 of agar; and the explant cotyledonary node was submitted to (0,00; 2,22; 4,44; 6,66; 8,88; 13,32; 17,76 and 22,20 μM), plus 30 gL-1 of sucrose and 7 gL-1 of agar. It was verified that the cotyledon node submitted to the concentration of 12,50 μM BAP is the best source of explant, with regeneration of the shoots via direct organogenesis, and the leaf is the most efficient explant for the indirect organogenesis in concentrations of 4,44 μM BAP. Histological analysis proved the pathways of regeneration. The shoots from the direct organogenesis were rooted in vitro in MS½ medium free of plant regulator, and the microplants acclimatized in vegetal soil, reached 100% survival at 90 days of acclimatization. Biochemical and phytochemical analyzes of stem, root and leaf explants were carried out in vitro and ex vitro. The analysis showed that in vitro cultivation favors the synthesis of proteins and reducer sugars in leaf and root tissues. The metabolite rutin is produced in leaf explants in greenhouse.Physalis peruviana L. (Solanaceae) é uma frutífera herbácea, que vem se consolidando no mercado de pequenas frutas em virtude das suas potencialidades nutricionais, medicinais e relevante concentração de vitaminas, sais minerais e metabólitos secundários de interesse à indústria farmacêutica. As limitações à sua produção são o ciclo reprodutivo curto, o acometimento dos frutos por pragas e a carência de estudos para a espécie. A micropropagação constitui-se numa alternativa para propagação de espécies medicinais, visto que esta técnica permite a produção de um grande número de mudas com qualidade fitossanitária, além de se constituir uma ferramenta para o melhoramento genético e para estudos fitoquímicos. Esse estudo se propôs a avaliar a morfogênese in vitro de P. peruviana e analisar o perfil bioquímico e fitoquímico da espécie em diferentes cultivos. Explantes de nó cotiledonar, cotilédone, folha, epicótilo, hipocótilo e raiz foram inoculados em meio de cultura MS com metade das concentrações salinas e suplementado com BAP (0,00; 2,22; 4,44; 6,66 e 8,88µM),acrescido de 30 g.L-1de sacarose e 7 g.L-1 de ágar; e o explante nó cotiledonar foi submetido à (0,00; 2,22; 4,44; 6,66; 8,88; 13,32; 17,76 e 22,20 µM). Verificou-se que o nó cotiledonar submetido à concentração de 12,50 µM de BAP é a melhor fonte de explante, com regeneração dos brotos via organogênese direta, e a folha é o explante mais eficiente para a organogênese indireta em concentrações de 4,44 µM de BAP. A análise histológica comprovou as vias de regeneração. Os brotos provenientes da organogênese direta foram enraizados in vitro em meio MS½ isento de regulador vegetal,e as microplantas aclimatizadas em terra vegetal, alcançaram 100% de sobrevivência aos 90 dias da aclimatização. Realizou-se análises bioquímicas e fitoquímicas dos explantes caule, raiz e folhas in vitro e ex vitro. As análises evidenciaram que o cultivo in vitro favorece a síntese de proteínas e açúcares redutores em tecidos de folhas e raízes. O metabólito rutina é produzido em explantes foliares em casa de vegetação.Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2019-05-07T21:16:49Z No. of bitstreams: 1 30.09 dissertação final. pdf. gráfica e CD.pdf: 1424025 bytes, checksum: a96c5b62a0afc9fb5ed6d6966d252e3a (MD5)Made available in DSpace on 2019-05-07T21:16:49Z (GMT). No. of bitstreams: 1 30.09 dissertação final. pdf. gráfica e CD.pdf: 1424025 bytes, checksum: a96c5b62a0afc9fb5ed6d6966d252e3a (MD5) Previous issue date: 2018-06-05Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Estadual de Feira de SantanaMestrado Acadêmico em Recursos Genéticos VegetaisUEFSBrasilDEPARTAMENTO DE CIÊNCIAS BIOLÓGICASCultura de tecidosOrganogêneseBAPMetabólitos secundáriosRutinaTissue cultureOrganogenesisSecondary metabolitesRutinCIENCIAS BIOLOGICAS::BIOLOGIA GERALMicropropagação e análises bioquímicas e fitoquímicas de Physalis peruviana L.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis81466464816222418746006006006005026123383450589282-16345593859312446973590462550136975366info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEFSinstname:Universidade Estadual de Feira de Santana (UEFS)instacron:UEFSORIGINAL30.09 dissertação final. pdf. gráfica e CD.pdf30.09 dissertação final. pdf. gráfica e CD.pdfapplication/pdf1424025http://tede2.uefs.br:8080/bitstream/tede/754/2/30.09+disserta%C3%A7%C3%A3o+final.+pdf.+gr%C3%A1fica+e+CD.pdfa96c5b62a0afc9fb5ed6d6966d252e3aMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82089http://tede2.uefs.br:8080/bitstream/tede/754/1/license.txt7b5ba3d2445355f386edab96125d42b7MD51tede/7542019-05-08 19:43:52.882oai:tede2.uefs.br:8080:tede/754Tk9UQTogQ09MT1FVRSBBUVVJIEEgU1VBIFBSP1BSSUEgTElDRU4/QQpFc3RhIGxpY2VuP2EgZGUgZXhlbXBsbyA/IGZvcm5lY2lkYSBhcGVuYXMgcGFyYSBmaW5zIGluZm9ybWF0aXZvcy4KCkxJQ0VOP0EgREUgRElTVFJJQlVJPz9PIE4/Ty1FWENMVVNJVkEKCkNvbSBhIGFwcmVzZW50YT8/byBkZXN0YSBsaWNlbj9hLCB2b2M/IChvIGF1dG9yIChlcykgb3UgbyB0aXR1bGFyIGRvcyBkaXJlaXRvcyBkZSBhdXRvcikgY29uY2VkZSA/IFVuaXZlcnNpZGFkZSAKWFhYIChTaWdsYSBkYSBVbml2ZXJzaWRhZGUpIG8gZGlyZWl0byBuP28tZXhjbHVzaXZvIGRlIHJlcHJvZHV6aXIsICB0cmFkdXppciAoY29uZm9ybWUgZGVmaW5pZG8gYWJhaXhvKSwgZS9vdSAKZGlzdHJpYnVpciBhIHN1YSB0ZXNlIG91IGRpc3NlcnRhPz9vIChpbmNsdWluZG8gbyByZXN1bW8pIHBvciB0b2RvIG8gbXVuZG8gbm8gZm9ybWF0byBpbXByZXNzbyBlIGVsZXRyP25pY28gZSAKZW0gcXVhbHF1ZXIgbWVpbywgaW5jbHVpbmRvIG9zIGZvcm1hdG9zID91ZGlvIG91IHY/ZGVvLgoKVm9jPyBjb25jb3JkYSBxdWUgYSBTaWdsYSBkZSBVbml2ZXJzaWRhZGUgcG9kZSwgc2VtIGFsdGVyYXIgbyBjb250ZT9kbywgdHJhbnNwb3IgYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YT8/byAKcGFyYSBxdWFscXVlciBtZWlvIG91IGZvcm1hdG8gcGFyYSBmaW5zIGRlIHByZXNlcnZhPz9vLgoKVm9jPyB0YW1iP20gY29uY29yZGEgcXVlIGEgU2lnbGEgZGUgVW5pdmVyc2lkYWRlIHBvZGUgbWFudGVyIG1haXMgZGUgdW1hIGM/cGlhIGEgc3VhIHRlc2Ugb3UgCmRpc3NlcnRhPz9vIHBhcmEgZmlucyBkZSBzZWd1cmFuP2EsIGJhY2stdXAgZSBwcmVzZXJ2YT8/by4KClZvYz8gZGVjbGFyYSBxdWUgYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YT8/byA/IG9yaWdpbmFsIGUgcXVlIHZvYz8gdGVtIG8gcG9kZXIgZGUgY29uY2VkZXIgb3MgZGlyZWl0b3MgY29udGlkb3MgCm5lc3RhIGxpY2VuP2EuIFZvYz8gdGFtYj9tIGRlY2xhcmEgcXVlIG8gZGVwP3NpdG8gZGEgc3VhIHRlc2Ugb3UgZGlzc2VydGE/P28gbj9vLCBxdWUgc2VqYSBkZSBzZXUgCmNvbmhlY2ltZW50bywgaW5mcmluZ2UgZGlyZWl0b3MgYXV0b3JhaXMgZGUgbmluZ3U/bS4KCkNhc28gYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YT8/byBjb250ZW5oYSBtYXRlcmlhbCBxdWUgdm9jPyBuP28gcG9zc3VpIGEgdGl0dWxhcmlkYWRlIGRvcyBkaXJlaXRvcyBhdXRvcmFpcywgdm9jPyAKZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzcz9vIGlycmVzdHJpdGEgZG8gZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzIHBhcmEgY29uY2VkZXIgPyBTaWdsYSBkZSBVbml2ZXJzaWRhZGUgCm9zIGRpcmVpdG9zIGFwcmVzZW50YWRvcyBuZXN0YSBsaWNlbj9hLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3Q/IGNsYXJhbWVudGUgCmlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIG5vIHRleHRvIG91IG5vIGNvbnRlP2RvIGRhIHRlc2Ugb3UgZGlzc2VydGE/P28gb3JhIGRlcG9zaXRhZGEuCgpDQVNPIEEgVEVTRSBPVSBESVNTRVJUQT8/TyBPUkEgREVQT1NJVEFEQSBURU5IQSBTSURPIFJFU1VMVEFETyBERSBVTSBQQVRST0M/TklPIE9VIApBUE9JTyBERSBVTUEgQUc/TkNJQSBERSBGT01FTlRPIE9VIE9VVFJPIE9SR0FOSVNNTyBRVUUgTj9PIFNFSkEgQSBTSUdMQSBERSAKVU5JVkVSU0lEQURFLCBWT0M/IERFQ0xBUkEgUVVFIFJFU1BFSVRPVSBUT0RPUyBFIFFVQUlTUVVFUiBESVJFSVRPUyBERSBSRVZJUz9PIENPTU8gClRBTUI/TSBBUyBERU1BSVMgT0JSSUdBPz9FUyBFWElHSURBUyBQT1IgQ09OVFJBVE8gT1UgQUNPUkRPLgoKQSBTaWdsYSBkZSBVbml2ZXJzaWRhZGUgc2UgY29tcHJvbWV0ZSBhIGlkZW50aWZpY2FyIGNsYXJhbWVudGUgbyBzZXUgbm9tZSAocykgb3UgbyhzKSBub21lKHMpIGRvKHMpIApkZXRlbnRvcihlcykgZG9zIGRpcmVpdG9zIGF1dG9yYWlzIGRhIHRlc2Ugb3UgZGlzc2VydGE/P28sIGUgbj9vIGZhcj8gcXVhbHF1ZXIgYWx0ZXJhPz9vLCBhbD9tIGRhcXVlbGFzIApjb25jZWRpZGFzIHBvciBlc3RhIGxpY2VuP2EuCg==Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.uefs.br:8080/PUBhttp://tede2.uefs.br:8080/oai/requestbcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.bropendoar:2019-05-08T22:43:52Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS)false |
dc.title.por.fl_str_mv |
Micropropagação e análises bioquímicas e fitoquímicas de Physalis peruviana L. |
title |
Micropropagação e análises bioquímicas e fitoquímicas de Physalis peruviana L. |
spellingShingle |
Micropropagação e análises bioquímicas e fitoquímicas de Physalis peruviana L. Mascarenhas, Lilian Marcia Santana Cultura de tecidos Organogênese BAP Metabólitos secundários Rutina Tissue culture Organogenesis Secondary metabolites Rutin CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
title_short |
Micropropagação e análises bioquímicas e fitoquímicas de Physalis peruviana L. |
title_full |
Micropropagação e análises bioquímicas e fitoquímicas de Physalis peruviana L. |
title_fullStr |
Micropropagação e análises bioquímicas e fitoquímicas de Physalis peruviana L. |
title_full_unstemmed |
Micropropagação e análises bioquímicas e fitoquímicas de Physalis peruviana L. |
title_sort |
Micropropagação e análises bioquímicas e fitoquímicas de Physalis peruviana L. |
author |
Mascarenhas, Lilian Marcia Santana |
author_facet |
Mascarenhas, Lilian Marcia Santana |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Brito, Alone Lima |
dc.contributor.advisor1ID.fl_str_mv |
52190404568 |
dc.contributor.authorID.fl_str_mv |
83508309568 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7398279864316232 |
dc.contributor.author.fl_str_mv |
Mascarenhas, Lilian Marcia Santana |
contributor_str_mv |
Brito, Alone Lima |
dc.subject.por.fl_str_mv |
Cultura de tecidos Organogênese BAP Metabólitos secundários Rutina |
topic |
Cultura de tecidos Organogênese BAP Metabólitos secundários Rutina Tissue culture Organogenesis Secondary metabolites Rutin CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
dc.subject.eng.fl_str_mv |
Tissue culture Organogenesis Secondary metabolites Rutin |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
description |
Physalis peruviana L. (Solanaceae) is a fruit herbaceous, which has been consolidating in the small fruit market due to its nutritional, medicinal potentialities and relevant concentration of vitamins, minerals and secondary metabolites of interest to the pharmaceutical industry. The barriers to its production are the short reproductive cycle, the involvement of the fruits by pests and the lack of studies for the species. Micropropagation is an alternative for the propagation of medicinal species, since this technique allows the production of a large number of seedlings with phytosanitary quality, besides being a tool for genetic improvement and for phytochemical studies. This study aims to evaluate the in vitro morphogenesis of P. peruviana and to analyze the biochemical and phytochemical profile of the species in different cultures. Cotyledon node, cotyledon, leaf, epicotyl, hypocotyl and root explants were inoculated in half-saline MS culture medium supplemented with BAP (0,00; 2,22; 4,44; 6,66 and 8,88µM), plus 30 gL-1 of sucrose and 7 gL-1 of agar; and the explant cotyledonary node was submitted to (0,00; 2,22; 4,44; 6,66; 8,88; 13,32; 17,76 and 22,20 μM), plus 30 gL-1 of sucrose and 7 gL-1 of agar. It was verified that the cotyledon node submitted to the concentration of 12,50 μM BAP is the best source of explant, with regeneration of the shoots via direct organogenesis, and the leaf is the most efficient explant for the indirect organogenesis in concentrations of 4,44 μM BAP. Histological analysis proved the pathways of regeneration. The shoots from the direct organogenesis were rooted in vitro in MS½ medium free of plant regulator, and the microplants acclimatized in vegetal soil, reached 100% survival at 90 days of acclimatization. Biochemical and phytochemical analyzes of stem, root and leaf explants were carried out in vitro and ex vitro. The analysis showed that in vitro cultivation favors the synthesis of proteins and reducer sugars in leaf and root tissues. The metabolite rutin is produced in leaf explants in greenhouse. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018-06-05 |
dc.date.accessioned.fl_str_mv |
2019-05-07T21:16:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MASCARENHAS, Lilian Marcia Santana. Micropropagação e análises bioquímicas e fitoquímicas de Physalis peruviana L. 2018. 75 f. Dissertação (Mestrado Acadêmico em Recursos Genéticos Vegetais) - Universidade Estadual de Feira de Santana, Feira de Santana, 2018. |
dc.identifier.uri.fl_str_mv |
http://tede2.uefs.br:8080/handle/tede/754 |
identifier_str_mv |
MASCARENHAS, Lilian Marcia Santana. Micropropagação e análises bioquímicas e fitoquímicas de Physalis peruviana L. 2018. 75 f. Dissertação (Mestrado Acadêmico em Recursos Genéticos Vegetais) - Universidade Estadual de Feira de Santana, Feira de Santana, 2018. |
url |
http://tede2.uefs.br:8080/handle/tede/754 |
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por |
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por |
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Universidade Estadual de Feira de Santana |
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UEFS |
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DEPARTAMENTO DE CIÊNCIAS BIOLÓGICAS |
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Universidade Estadual de Feira de Santana |
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