Indução e caracterização de calos de Amburana cearensis (Allen.) A.C E Poincianella pyramidalis (Tul.) L.P.Queiroz
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UEFS |
Texto Completo: | http://tede2.uefs.br:8080/handle/tede/781 |
Resumo: | The objective of this work was to establish a protocol for the induction and initiation of somatic embryogenesis of Amburana cearensis (amburana) and Poincianella Pyramidali (catingueira), establishing adequate time for callus repication and characterizing them as morphological aspects, besides performing the analysis phytochemistry of the catingueira. For the callus induction, leaf segments of amburana and cotyledonary segments for the catingueira were used as explants, all with about 1.0 cm2 of plants previously germinated in vitro. For amburana, explants were cultured in WPM medium supplemented with different concentrations of 2,4-D or picloram (0.0, 5.0, 10.0, 20.0 and 40.0 μM). for the catingueira, explants were cultured in MS / 2 medium supplemented with different associations of picloram (0.0, 2.5, 5.0, 10.0 and 20.0 μM) x BAP (0.05, 1.0 , 5.0, 10.0 μM).After 30 days of in vitro culture, the percentage of calli formed was evaluated, the growth curve of both species was determined by the fresh weight (g) of the calli. Phytochemical screening was performed to establish the phytochemical profile of catingueira extracts grown in different environments. The regression analysis showed a quadratic response for callus production for both species. After 30 days the best callus production for the amburana species was observed in the concentration of 23.83 μM of 2,4-D with 84,71% of callus formed. For the catingueira species the highest production was observed in the presence of 19,53 μM picloram + 10 μM BAP with 98.68% callus formation.Callus with friable characteristics, as well as the presence of the apigenin compound in catingueira callus suggests future work for the in vitro production of these species and the exploitation of these compounds through biotechnological techniques. |
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Santana, José Raniere Ferreira de09746602489http://lattes.cnpq.br/9012703045524286Costa, Evelyn Sophia Silva2019-06-12T00:14:16Z2018-03-20COSTA, Evelyn Sophia Silva. Indução e caracterização de calos de Amburana cearensis (Allen.) A.C E Poincianella pyramidalis (Tul.) L.P.Queiroz. 2018. 27 f. Dissertação (Mestrado Acadêmico em Recursos Genéticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2018.http://tede2.uefs.br:8080/handle/tede/781The objective of this work was to establish a protocol for the induction and initiation of somatic embryogenesis of Amburana cearensis (amburana) and Poincianella Pyramidali (catingueira), establishing adequate time for callus repication and characterizing them as morphological aspects, besides performing the analysis phytochemistry of the catingueira. For the callus induction, leaf segments of amburana and cotyledonary segments for the catingueira were used as explants, all with about 1.0 cm2 of plants previously germinated in vitro. For amburana, explants were cultured in WPM medium supplemented with different concentrations of 2,4-D or picloram (0.0, 5.0, 10.0, 20.0 and 40.0 μM). for the catingueira, explants were cultured in MS / 2 medium supplemented with different associations of picloram (0.0, 2.5, 5.0, 10.0 and 20.0 μM) x BAP (0.05, 1.0 , 5.0, 10.0 μM).After 30 days of in vitro culture, the percentage of calli formed was evaluated, the growth curve of both species was determined by the fresh weight (g) of the calli. Phytochemical screening was performed to establish the phytochemical profile of catingueira extracts grown in different environments. The regression analysis showed a quadratic response for callus production for both species. After 30 days the best callus production for the amburana species was observed in the concentration of 23.83 μM of 2,4-D with 84,71% of callus formed. For the catingueira species the highest production was observed in the presence of 19,53 μM picloram + 10 μM BAP with 98.68% callus formation.Callus with friable characteristics, as well as the presence of the apigenin compound in catingueira callus suggests future work for the in vitro production of these species and the exploitation of these compounds through biotechnological techniques.Objetivou-se com este trabalho estabelecer um protocolo para a indução e iniciação da embriogênese somática de Amburana cearensis (amburana) ePoincianella Pyramidali(catingueira), estabelecendo o tempo adequado para a repicagem dos calos e caracteriza-los quanto os aspectos morfológicos, além de realizar a análise fitoquímica da catingueira. Para a indução de calos foram utilizados como explantes segmentos foliares de amburana e para a espécie catingueira, segmentos cotiledonares, todos com cerca de 1,0 cm2 provenientes de plantas previamente germinadas in vitro. Para a amburana os explantes foram cultivados em meio WPM suplementados com diferentes concentrações de 2,4-D ou picloram (0,0; 5,0; 10,0; 20,0 e 40,0 µM), já para a catingueira os explantes foram cultivados em meio MS/2 suplementados com diferentes associações de picloram (0,0; 2,5; 5,0; 10,0 e 20,0 µM) x BAP (0,0; 0,5; 1,0; 2,5; 5,0; 10,0 µM). Após 30 dias de cultivo in vitro, avaliou-se a porcentagem de calos formados, a curva de crescimento de ambas as espécies foi determinada através do peso fresco (g) dos calos. A triagem fitoquímica foram realizados para estabelecer o perfil fitoquimico dos extratos de catingueira cultivados em diferentes ambientes. A analise de regressão apresentou resposta quadrática para a produção de calos para ambas as espécies. Após 30 dias a melhor produção de calos para a espécie amburana foi observado na concentração de 23,83µM de 2,4-D com 84,71% de calos formados, já para a espécie catingueira a maior produção foi observado na presença de 19,53µM de picloram + 10µM de BAP com 98,68% de calos formados. Os calos apresentaram crescimento sigmoidal e características friáveis para ambas as espécies. Foi possível identificar pela análise fitoquímica a presença de apigenina. Calos com características friáveis, assim como, a presença do composto apigenina nos calos de catingueira sugere trabalhos futuros para a produção in vitro dessas espécies e a exploração desse compostos através de técnicas biotecnológicas.Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2019-06-12T00:14:16Z No. of bitstreams: 1 PDF INDUÇÃO E CARACTERIZAÇÃO DE CALOS (Evelyn Sophia).pdf: 1296402 bytes, checksum: 19305d6900d6b1525fd0325a9a334ed7 (MD5)Made available in DSpace on 2019-06-12T00:14:16Z (GMT). No. of bitstreams: 1 PDF INDUÇÃO E CARACTERIZAÇÃO DE CALOS (Evelyn Sophia).pdf: 1296402 bytes, checksum: 19305d6900d6b1525fd0325a9a334ed7 (MD5) Previous issue date: 2018-03-20Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Estadual de Feira de SantanaMestrado Acadêmico em Recursos Genéticos VegetaisUEFSBrasilDEPARTAMENTO DE CIÊNCIAS BIOLÓGICASCumaruCatingueiraCalogêneseAnatomia vegetalMetabolitos secundáriosCalogenesisPlant anatomySecondary metabolitesCIENCIAS BIOLOGICAS::BIOLOGIA GERALIndução e caracterização de calos de Amburana cearensis (Allen.) A.C E Poincianella pyramidalis (Tul.) 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dc.title.por.fl_str_mv |
Indução e caracterização de calos de Amburana cearensis (Allen.) A.C E Poincianella pyramidalis (Tul.) L.P.Queiroz |
title |
Indução e caracterização de calos de Amburana cearensis (Allen.) A.C E Poincianella pyramidalis (Tul.) L.P.Queiroz |
spellingShingle |
Indução e caracterização de calos de Amburana cearensis (Allen.) A.C E Poincianella pyramidalis (Tul.) L.P.Queiroz Costa, Evelyn Sophia Silva Cumaru Catingueira Calogênese Anatomia vegetal Metabolitos secundários Calogenesis Plant anatomy Secondary metabolites CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
title_short |
Indução e caracterização de calos de Amburana cearensis (Allen.) A.C E Poincianella pyramidalis (Tul.) L.P.Queiroz |
title_full |
Indução e caracterização de calos de Amburana cearensis (Allen.) A.C E Poincianella pyramidalis (Tul.) L.P.Queiroz |
title_fullStr |
Indução e caracterização de calos de Amburana cearensis (Allen.) A.C E Poincianella pyramidalis (Tul.) L.P.Queiroz |
title_full_unstemmed |
Indução e caracterização de calos de Amburana cearensis (Allen.) A.C E Poincianella pyramidalis (Tul.) L.P.Queiroz |
title_sort |
Indução e caracterização de calos de Amburana cearensis (Allen.) A.C E Poincianella pyramidalis (Tul.) L.P.Queiroz |
author |
Costa, Evelyn Sophia Silva |
author_facet |
Costa, Evelyn Sophia Silva |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Santana, José Raniere Ferreira de |
dc.contributor.authorID.fl_str_mv |
09746602489 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/9012703045524286 |
dc.contributor.author.fl_str_mv |
Costa, Evelyn Sophia Silva |
contributor_str_mv |
Santana, José Raniere Ferreira de |
dc.subject.por.fl_str_mv |
Cumaru Catingueira Calogênese Anatomia vegetal Metabolitos secundários |
topic |
Cumaru Catingueira Calogênese Anatomia vegetal Metabolitos secundários Calogenesis Plant anatomy Secondary metabolites CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
dc.subject.eng.fl_str_mv |
Calogenesis Plant anatomy Secondary metabolites |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
description |
The objective of this work was to establish a protocol for the induction and initiation of somatic embryogenesis of Amburana cearensis (amburana) and Poincianella Pyramidali (catingueira), establishing adequate time for callus repication and characterizing them as morphological aspects, besides performing the analysis phytochemistry of the catingueira. For the callus induction, leaf segments of amburana and cotyledonary segments for the catingueira were used as explants, all with about 1.0 cm2 of plants previously germinated in vitro. For amburana, explants were cultured in WPM medium supplemented with different concentrations of 2,4-D or picloram (0.0, 5.0, 10.0, 20.0 and 40.0 μM). for the catingueira, explants were cultured in MS / 2 medium supplemented with different associations of picloram (0.0, 2.5, 5.0, 10.0 and 20.0 μM) x BAP (0.05, 1.0 , 5.0, 10.0 μM).After 30 days of in vitro culture, the percentage of calli formed was evaluated, the growth curve of both species was determined by the fresh weight (g) of the calli. Phytochemical screening was performed to establish the phytochemical profile of catingueira extracts grown in different environments. The regression analysis showed a quadratic response for callus production for both species. After 30 days the best callus production for the amburana species was observed in the concentration of 23.83 μM of 2,4-D with 84,71% of callus formed. For the catingueira species the highest production was observed in the presence of 19,53 μM picloram + 10 μM BAP with 98.68% callus formation.Callus with friable characteristics, as well as the presence of the apigenin compound in catingueira callus suggests future work for the in vitro production of these species and the exploitation of these compounds through biotechnological techniques. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018-03-20 |
dc.date.accessioned.fl_str_mv |
2019-06-12T00:14:16Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
COSTA, Evelyn Sophia Silva. Indução e caracterização de calos de Amburana cearensis (Allen.) A.C E Poincianella pyramidalis (Tul.) L.P.Queiroz. 2018. 27 f. Dissertação (Mestrado Acadêmico em Recursos Genéticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2018. |
dc.identifier.uri.fl_str_mv |
http://tede2.uefs.br:8080/handle/tede/781 |
identifier_str_mv |
COSTA, Evelyn Sophia Silva. Indução e caracterização de calos de Amburana cearensis (Allen.) A.C E Poincianella pyramidalis (Tul.) L.P.Queiroz. 2018. 27 f. Dissertação (Mestrado Acadêmico em Recursos Genéticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2018. |
url |
http://tede2.uefs.br:8080/handle/tede/781 |
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por |
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openAccess |
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Universidade Estadual de Feira de Santana |
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UEFS |
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DEPARTAMENTO DE CIÊNCIAS BIOLÓGICAS |
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Universidade Estadual de Feira de Santana |
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