Morfogênese e conservação in vitro de Caesalpinia pyramidalis Tul.
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UEFS |
Texto Completo: | http://tede2.uefs.br:8080/handle/tede/1023 |
Resumo: | The catingueira (Caesalpinia pyramidalis Tul.) is an endemic species of the Caatinga, which has the potential timber, fodder and medicinal. The plant tissue culture has proved effective in multiplication and conservation of woody species with potential explored. Thus, this study investigated the effects of the types and concentrations of plant growth regulators on in vitro multiplication of C. pyramidalis and develop a protocol for in vitro conservation of the same. For multiplication was carried out three experiments in which we assessed the effects of different concentrations of plant growth regulators (BAP, KIN, TDZ or NAA) on different explants (hypocotyls, cotyledon segments, cotyledons, and epicotyl nodal segments). For the conservation in vitro experiments there were two: firstly tested the effect of different concentrations of sucrose (87.64, 131.46, 175.28 and 219.10mM) supplemented with different concentrations (0.0 and 87.64mM) of sorbitol or mannitol, and second, to evaluate the use of different concentrations (0.0, 1.5, 3.0, 4.5 and 6.0µM) the growth retardant paclobutrazol (PBZ). The culture medium used for all experiments was the WPM. The highest rate of regeneration, shoot number, leaf number, shoot length and dry weight of shoots were obtained from the nodal segments were inoculated into plant growth regulator free medium. The use of mannitol and sorbitol were not efficient for in vitro conservation catingueira, being suitable for this purpose, use of concentration of 219.10mM sucrose and 6μM PBZ until 240 days. |
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Santana, José Raniere Ferreira de5515544854902068787539http://lattes.cnpq.br/5228799189641294Silva, Tecla Dos Santos2020-04-04T17:41:08Z2012-02-28SILVA, Tecla dos Santos. Morfogênese e conservação in vitro de Caesalpinia pyramidalis Tul. 2012. 88 f. Dissertação (Mestrado Acadêmico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2012.http://tede2.uefs.br:8080/handle/tede/1023The catingueira (Caesalpinia pyramidalis Tul.) is an endemic species of the Caatinga, which has the potential timber, fodder and medicinal. The plant tissue culture has proved effective in multiplication and conservation of woody species with potential explored. Thus, this study investigated the effects of the types and concentrations of plant growth regulators on in vitro multiplication of C. pyramidalis and develop a protocol for in vitro conservation of the same. For multiplication was carried out three experiments in which we assessed the effects of different concentrations of plant growth regulators (BAP, KIN, TDZ or NAA) on different explants (hypocotyls, cotyledon segments, cotyledons, and epicotyl nodal segments). For the conservation in vitro experiments there were two: firstly tested the effect of different concentrations of sucrose (87.64, 131.46, 175.28 and 219.10mM) supplemented with different concentrations (0.0 and 87.64mM) of sorbitol or mannitol, and second, to evaluate the use of different concentrations (0.0, 1.5, 3.0, 4.5 and 6.0µM) the growth retardant paclobutrazol (PBZ). The culture medium used for all experiments was the WPM. The highest rate of regeneration, shoot number, leaf number, shoot length and dry weight of shoots were obtained from the nodal segments were inoculated into plant growth regulator free medium. The use of mannitol and sorbitol were not efficient for in vitro conservation catingueira, being suitable for this purpose, use of concentration of 219.10mM sucrose and 6μM PBZ until 240 days.A catingueira (Caesalpinia pyramidalis Tul.) é uma espécie endêmica da Caatinga, que possui potencial madeireiro, forrageiro e medicinal. A cultura de tecidos vegetais tem-se mostrado eficaz na multiplicação e conservação de espécies lenhosas com potenciais explorados. Dessa forma, este trabalho objetivou estudar os efeitos dos tipos e concentrações de reguladores de crescimento vegetal sobre a multiplicação in vitro de C. pyramidalis e desenvolver um protocolo de conservação in vitro para a mesma. Para a multiplicação realizou-se três experimentos, onde foram avaliados os efeitos de diferentes concentrações dos reguladores de crescimento vegetal (BAP, KIN, TDZ e ANA) sobre diferentes explantes (hipocótilo, segmento cotiledonar, cotilédone, epicótilo e segmento nodal). Para a conservação in vitro, realizaram-se dois experimentos: no primeiro testou-se o efeito de diferentes concentrações de sacarose (87,64; 131,46; 175,28 e 219,10mM) combinados com concentrações (0,0 e 87,64mM) de sorbitol ou manitol, e no segundo, avaliou-se o uso de diferentes concentrações (0,0; 1,5; 3,0; 4,5 e 6,0µM) do retardante de crescimento paclobutrazol (PBZ). O meio de cultura usado para todos os experimentos foi o WPM. A maior taxa de regeneração, número de brotos, número de folhas, comprimento da parte aérea e matéria seca da parte aérea foram obtidas com o segmento nodal inoculado em meio isento de regulador de crescimento vegetal. O uso do sorbitol e do manitol não se mostraram eficientes na conservação in vitro da catingueira, sendo indicada para este propósito, a utilização da concentração de 219,10mM de sacarose e de 6µM de PBZ por até 240 dias.Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2020-04-04T17:41:08Z No. of bitstreams: 1 Dissertação_ Tecla dos Santos.pdf: 5784958 bytes, checksum: ee38009be8880e08442aae1467fb456f (MD5)Made available in DSpace on 2020-04-04T17:41:08Z (GMT). No. of bitstreams: 1 Dissertação_ Tecla dos Santos.pdf: 5784958 bytes, checksum: ee38009be8880e08442aae1467fb456f (MD5) Previous issue date: 2012-02-28Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Estadual de Feira de SantanaMestrado Acadêmico em BiotecnologiaUEFSBrasilDEPARTAMENTO DE CIÊNCIAS BIOLÓGICASAgente osmóticoRetardante de crescimentoReguladores de crescimento vegetalOsmotic agentGrowth retardantPlant growth regulatorsCIENCIAS BIOLOGICAS::BIOLOGIA GERALCIENCIAS BIOLOGICASMorfogênese e conservação in vitro de Caesalpinia pyramidalis Tul.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-54735432730516527826006006006006005026123383450589282-1634559385931244697-34391788430682021613590462550136975366info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEFSinstname:Universidade Estadual de Feira de Santana (UEFS)instacron:UEFSORIGINALDissertação_ Tecla dos Santos.pdfDissertação_ Tecla dos Santos.pdfapplication/pdf5784958http://tede2.uefs.br:8080/bitstream/tede/1023/2/Disserta%C3%A7%C3%A3o_+Tecla+dos+Santos.pdfee38009be8880e08442aae1467fb456fMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82089http://tede2.uefs.br:8080/bitstream/tede/1023/1/license.txt7b5ba3d2445355f386edab96125d42b7MD51tede/10232020-04-04 14:41:08.876oai:tede2.uefs.br:8080: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.uefs.br:8080/PUBhttp://tede2.uefs.br:8080/oai/requestbcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.bropendoar:2020-04-04T17:41:08Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS)false |
dc.title.por.fl_str_mv |
Morfogênese e conservação in vitro de Caesalpinia pyramidalis Tul. |
title |
Morfogênese e conservação in vitro de Caesalpinia pyramidalis Tul. |
spellingShingle |
Morfogênese e conservação in vitro de Caesalpinia pyramidalis Tul. Silva, Tecla Dos Santos Agente osmótico Retardante de crescimento Reguladores de crescimento vegetal Osmotic agent Growth retardant Plant growth regulators CIENCIAS BIOLOGICAS::BIOLOGIA GERAL CIENCIAS BIOLOGICAS |
title_short |
Morfogênese e conservação in vitro de Caesalpinia pyramidalis Tul. |
title_full |
Morfogênese e conservação in vitro de Caesalpinia pyramidalis Tul. |
title_fullStr |
Morfogênese e conservação in vitro de Caesalpinia pyramidalis Tul. |
title_full_unstemmed |
Morfogênese e conservação in vitro de Caesalpinia pyramidalis Tul. |
title_sort |
Morfogênese e conservação in vitro de Caesalpinia pyramidalis Tul. |
author |
Silva, Tecla Dos Santos |
author_facet |
Silva, Tecla Dos Santos |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Santana, José Raniere Ferreira de |
dc.contributor.advisor1ID.fl_str_mv |
55155448549 |
dc.contributor.authorID.fl_str_mv |
02068787539 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5228799189641294 |
dc.contributor.author.fl_str_mv |
Silva, Tecla Dos Santos |
contributor_str_mv |
Santana, José Raniere Ferreira de |
dc.subject.por.fl_str_mv |
Agente osmótico Retardante de crescimento Reguladores de crescimento vegetal |
topic |
Agente osmótico Retardante de crescimento Reguladores de crescimento vegetal Osmotic agent Growth retardant Plant growth regulators CIENCIAS BIOLOGICAS::BIOLOGIA GERAL CIENCIAS BIOLOGICAS |
dc.subject.eng.fl_str_mv |
Osmotic agent Growth retardant Plant growth regulators |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL CIENCIAS BIOLOGICAS |
description |
The catingueira (Caesalpinia pyramidalis Tul.) is an endemic species of the Caatinga, which has the potential timber, fodder and medicinal. The plant tissue culture has proved effective in multiplication and conservation of woody species with potential explored. Thus, this study investigated the effects of the types and concentrations of plant growth regulators on in vitro multiplication of C. pyramidalis and develop a protocol for in vitro conservation of the same. For multiplication was carried out three experiments in which we assessed the effects of different concentrations of plant growth regulators (BAP, KIN, TDZ or NAA) on different explants (hypocotyls, cotyledon segments, cotyledons, and epicotyl nodal segments). For the conservation in vitro experiments there were two: firstly tested the effect of different concentrations of sucrose (87.64, 131.46, 175.28 and 219.10mM) supplemented with different concentrations (0.0 and 87.64mM) of sorbitol or mannitol, and second, to evaluate the use of different concentrations (0.0, 1.5, 3.0, 4.5 and 6.0µM) the growth retardant paclobutrazol (PBZ). The culture medium used for all experiments was the WPM. The highest rate of regeneration, shoot number, leaf number, shoot length and dry weight of shoots were obtained from the nodal segments were inoculated into plant growth regulator free medium. The use of mannitol and sorbitol were not efficient for in vitro conservation catingueira, being suitable for this purpose, use of concentration of 219.10mM sucrose and 6μM PBZ until 240 days. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-02-28 |
dc.date.accessioned.fl_str_mv |
2020-04-04T17:41:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, Tecla dos Santos. Morfogênese e conservação in vitro de Caesalpinia pyramidalis Tul. 2012. 88 f. Dissertação (Mestrado Acadêmico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2012. |
dc.identifier.uri.fl_str_mv |
http://tede2.uefs.br:8080/handle/tede/1023 |
identifier_str_mv |
SILVA, Tecla dos Santos. Morfogênese e conservação in vitro de Caesalpinia pyramidalis Tul. 2012. 88 f. Dissertação (Mestrado Acadêmico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2012. |
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http://tede2.uefs.br:8080/handle/tede/1023 |
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por |
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por |
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Universidade Estadual de Feira de Santana |
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UEFS |
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Universidade Estadual de Feira de Santana |
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