Micropropagação de duas espécies de veloziaceae da Chapada Diamantina
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UEFS |
Texto Completo: | http://tede2.uefs.br:8080/handle/tede/1517 |
Resumo: | Velloziaceae is one of the main families of the floristic composition of the rupestrian fields of Chapada Diamantina and has species with significant ornamental potential and resistance to climate change. The species Vellozia seubertiana and Vellozia pyrantha stand out for the beauty of the flowers and lack studies to guarantee their propagation and conservation. This work had as objective to establish protocols of micropropagation of the species V. seubertiana and V. pyrantha. For establishment in vitro the seeds of the two species were disinfected and inoculated in MS medium with half the saline concentrations and activated charcoal 1 g L-¹. Whole germinated plants in vitro were used for the induction of shoots in MS ½ medium supplemented with BAP (0.00; 4.44; 8.88 and 17.76 µM) and ANA (0.00 and 2 .22 µM). For V. seubertiana the influence of the consistency of the cultivation medium (solid and liquid) in the multiplication stepwas tested. Shoots of V. seubertiana were inoculated in MS ½ medium containing activated charcoal (0.0 and 1.0 g L-¹) and IBA (0.00 and 2.22 µM) for rooting. Rooted Microplantas had been aclimatized in vegetation house. It was obtained, via direct organogenesis, an average of 5.7 sprouts of V. seubertiana in a medium containing 8.75 µM of BAP and 2.22 µM of ANA and 5.5 sprouts per explant for the species V. pyrantha in medium with 4.44 µM of BAP. The solid medium was more efficient for the multiplication of V. seubertiana. In the rooting phase of V. seubertiana, the presence of activated carbon provided greater growth of the aerial part and root. ex vitro. It is concluded that micropropagation is a promising technique for the species V. seubertiana and V. pyrantha. |
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Brito, Alone Lima52190404568http://lattes.cnpq.br/056033951400627501828804517http://lattes.cnpq.br/0751257050352727Pinto, Dinah Ise Jimenez Gonçalves e Costa2023-08-14T13:17:56Z2021-09-26PINTO, Dinah Ise Jimenez Gonçalves e Costa. Micropropagação de duas espécies de veloziaceae da Chapada Diamantina. 2021. 49 f. Dissertação (Mestrado Acadêmico em Recursos Genéticos Vegetais) Universidade Estadual de Feira de Santana, Feira de Santana, 2021.http://tede2.uefs.br:8080/handle/tede/1517Velloziaceae is one of the main families of the floristic composition of the rupestrian fields of Chapada Diamantina and has species with significant ornamental potential and resistance to climate change. The species Vellozia seubertiana and Vellozia pyrantha stand out for the beauty of the flowers and lack studies to guarantee their propagation and conservation. This work had as objective to establish protocols of micropropagation of the species V. seubertiana and V. pyrantha. For establishment in vitro the seeds of the two species were disinfected and inoculated in MS medium with half the saline concentrations and activated charcoal 1 g L-¹. Whole germinated plants in vitro were used for the induction of shoots in MS ½ medium supplemented with BAP (0.00; 4.44; 8.88 and 17.76 µM) and ANA (0.00 and 2 .22 µM). For V. seubertiana the influence of the consistency of the cultivation medium (solid and liquid) in the multiplication stepwas tested. Shoots of V. seubertiana were inoculated in MS ½ medium containing activated charcoal (0.0 and 1.0 g L-¹) and IBA (0.00 and 2.22 µM) for rooting. Rooted Microplantas had been aclimatized in vegetation house. It was obtained, via direct organogenesis, an average of 5.7 sprouts of V. seubertiana in a medium containing 8.75 µM of BAP and 2.22 µM of ANA and 5.5 sprouts per explant for the species V. pyrantha in medium with 4.44 µM of BAP. The solid medium was more efficient for the multiplication of V. seubertiana. In the rooting phase of V. seubertiana, the presence of activated carbon provided greater growth of the aerial part and root. ex vitro. It is concluded that micropropagation is a promising technique for the species V. seubertiana and V. pyrantha.Velloziaceae é uma das principais famílias da composição florística dos campos rupestres da Chapada Diamantina e possui espécies com significativo potencial ornamental e resistência a mudanças climáticas. As espécies Vellozia seubertiana e Vellozia pyrantha se destacam pela beleza das suas flores e carecem de estudos que garantam a sua propagação e conservação. Este trabalho teve como objetivo estabelecer protocolos de micropropagação das espécies V. seubertiana e V. pyrantha. Para o estabelecimento in vitro as sementes das duas espécies foram desinfestadas e inoculadas em meio MS com metade das concentrações salinas (MS ½) contendo 1 g L-¹ de carvão ativado a parte aérea das plantas germinadas in vitro foram utilizadas para a indução de brotos em meio MS ½ suplementado com BAP (0,00; 4,44; 8,88 e 17,76 µM) e ANA (0,00 e 2,22 µM). Para V. seubertiana testou-se também a influência da consistência do meio de cultura (sólido e líquido) na etapa de multiplicação. Brotos de V. seubertiana foram inoculados em meio MS ½ contendo carvão ativado (0,0 e 1,0 g L-¹) e AIB (0,00 e 2,22 µM) para enraizamento. Microplantas enraizadas foram aclimatizada sem casa de vegetação. Obteve-se, via organogênese direta, uma média de 5,7 brotos de V. seubertiana em meio contendo 8,75 µM de BAP e 2,22 µM de ANA e 5,5 brotos por explante para a espécie V. pyrantha em meio com 4,44 µM de BAP. O meio sólido foi mais eficiente para a multiplicação de V. seubertiana. Na fase de enraizamento de V. seubertiana, a presença do carvão ativado proporcionou maior crescimento da parte aérea e da raiz; e as plantas aclimatizadas atingiram 75%de sobrevivência após 60 dias da transferência para a condição ex vitro. Conclui-se que a micropropagação é uma técnica promissora para as espécies V. seubertiana e a V. pyrantha.Submitted by Amanda Ponce (aponce@uefs.br) on 2023-08-14T13:17:56Z No. of bitstreams: 1 VERSAO_FINAL___DINAH_ISE_JIMENEZ_GONCALVES.pdf: 898754 bytes, checksum: 90667d97a24c4685e19b9883b8003225 (MD5)Made available in DSpace on 2023-08-14T13:17:56Z (GMT). No. of bitstreams: 1 VERSAO_FINAL___DINAH_ISE_JIMENEZ_GONCALVES.pdf: 898754 bytes, checksum: 90667d97a24c4685e19b9883b8003225 (MD5) Previous issue date: 2021-09-26Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Estadual de Feira de SantanaMestrado Acadêmico em Recursos Genéticos VegetaisUEFSBrasilDEPARTAMENTO DE CIÊNCIAS BIOLÓGICASCampo rupestrePropagação in vitroRegulador vegetalCampo rupestreIn vitro propagationVegetable regulatorCIENCIAS BIOLOGICAS::BIOLOGIA GERALCIENCIAS BIOLOGICAS::BOTANICAFITOTECNIA::FISIOLOGIA DE PLANTAS CULTIVADASMicropropagação de duas espécies de veloziaceae da Chapada Diamantinainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis2441563959449389152600600600600600600-6971480722008537872-1634559385931244697-3406147892414307501-27695799023176980572075167498588264571info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEFSinstname:Universidade Estadual de Feira de Santana (UEFS)instacron:UEFSORIGINALVERSAO_FINAL___DINAH_ISE_JIMENEZ_GONCALVES.pdfVERSAO_FINAL___DINAH_ISE_JIMENEZ_GONCALVES.pdfapplication/pdf898754http://tede2.uefs.br:8080/bitstream/tede/1517/2/VERSAO_FINAL___DINAH_ISE_JIMENEZ_GONCALVES.pdf90667d97a24c4685e19b9883b8003225MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://tede2.uefs.br:8080/bitstream/tede/1517/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/15172023-08-14 10:17:56.484oai:tede2.uefs.br:8080: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.uefs.br:8080/PUBhttp://tede2.uefs.br:8080/oai/requestbcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.bropendoar:2023-08-14T13:17:56Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS)false |
dc.title.por.fl_str_mv |
Micropropagação de duas espécies de veloziaceae da Chapada Diamantina |
title |
Micropropagação de duas espécies de veloziaceae da Chapada Diamantina |
spellingShingle |
Micropropagação de duas espécies de veloziaceae da Chapada Diamantina Pinto, Dinah Ise Jimenez Gonçalves e Costa Campo rupestre Propagação in vitro Regulador vegetal Campo rupestre In vitro propagation Vegetable regulator CIENCIAS BIOLOGICAS::BIOLOGIA GERAL CIENCIAS BIOLOGICAS::BOTANICA FITOTECNIA::FISIOLOGIA DE PLANTAS CULTIVADAS |
title_short |
Micropropagação de duas espécies de veloziaceae da Chapada Diamantina |
title_full |
Micropropagação de duas espécies de veloziaceae da Chapada Diamantina |
title_fullStr |
Micropropagação de duas espécies de veloziaceae da Chapada Diamantina |
title_full_unstemmed |
Micropropagação de duas espécies de veloziaceae da Chapada Diamantina |
title_sort |
Micropropagação de duas espécies de veloziaceae da Chapada Diamantina |
author |
Pinto, Dinah Ise Jimenez Gonçalves e Costa |
author_facet |
Pinto, Dinah Ise Jimenez Gonçalves e Costa |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Brito, Alone Lima |
dc.contributor.advisor1ID.fl_str_mv |
52190404568 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/0560339514006275 |
dc.contributor.authorID.fl_str_mv |
01828804517 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0751257050352727 |
dc.contributor.author.fl_str_mv |
Pinto, Dinah Ise Jimenez Gonçalves e Costa |
contributor_str_mv |
Brito, Alone Lima |
dc.subject.por.fl_str_mv |
Campo rupestre Propagação in vitro Regulador vegetal |
topic |
Campo rupestre Propagação in vitro Regulador vegetal Campo rupestre In vitro propagation Vegetable regulator CIENCIAS BIOLOGICAS::BIOLOGIA GERAL CIENCIAS BIOLOGICAS::BOTANICA FITOTECNIA::FISIOLOGIA DE PLANTAS CULTIVADAS |
dc.subject.eng.fl_str_mv |
Campo rupestre In vitro propagation Vegetable regulator |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL CIENCIAS BIOLOGICAS::BOTANICA FITOTECNIA::FISIOLOGIA DE PLANTAS CULTIVADAS |
description |
Velloziaceae is one of the main families of the floristic composition of the rupestrian fields of Chapada Diamantina and has species with significant ornamental potential and resistance to climate change. The species Vellozia seubertiana and Vellozia pyrantha stand out for the beauty of the flowers and lack studies to guarantee their propagation and conservation. This work had as objective to establish protocols of micropropagation of the species V. seubertiana and V. pyrantha. For establishment in vitro the seeds of the two species were disinfected and inoculated in MS medium with half the saline concentrations and activated charcoal 1 g L-¹. Whole germinated plants in vitro were used for the induction of shoots in MS ½ medium supplemented with BAP (0.00; 4.44; 8.88 and 17.76 µM) and ANA (0.00 and 2 .22 µM). For V. seubertiana the influence of the consistency of the cultivation medium (solid and liquid) in the multiplication stepwas tested. Shoots of V. seubertiana were inoculated in MS ½ medium containing activated charcoal (0.0 and 1.0 g L-¹) and IBA (0.00 and 2.22 µM) for rooting. Rooted Microplantas had been aclimatized in vegetation house. It was obtained, via direct organogenesis, an average of 5.7 sprouts of V. seubertiana in a medium containing 8.75 µM of BAP and 2.22 µM of ANA and 5.5 sprouts per explant for the species V. pyrantha in medium with 4.44 µM of BAP. The solid medium was more efficient for the multiplication of V. seubertiana. In the rooting phase of V. seubertiana, the presence of activated carbon provided greater growth of the aerial part and root. ex vitro. It is concluded that micropropagation is a promising technique for the species V. seubertiana and V. pyrantha. |
publishDate |
2021 |
dc.date.issued.fl_str_mv |
2021-09-26 |
dc.date.accessioned.fl_str_mv |
2023-08-14T13:17:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
PINTO, Dinah Ise Jimenez Gonçalves e Costa. Micropropagação de duas espécies de veloziaceae da Chapada Diamantina. 2021. 49 f. Dissertação (Mestrado Acadêmico em Recursos Genéticos Vegetais) Universidade Estadual de Feira de Santana, Feira de Santana, 2021. |
dc.identifier.uri.fl_str_mv |
http://tede2.uefs.br:8080/handle/tede/1517 |
identifier_str_mv |
PINTO, Dinah Ise Jimenez Gonçalves e Costa. Micropropagação de duas espécies de veloziaceae da Chapada Diamantina. 2021. 49 f. Dissertação (Mestrado Acadêmico em Recursos Genéticos Vegetais) Universidade Estadual de Feira de Santana, Feira de Santana, 2021. |
url |
http://tede2.uefs.br:8080/handle/tede/1517 |
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Universidade Estadual de Feira de Santana |
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Universidade Estadual de Feira de Santana |
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