Indução de calos em anteras e poliploidia em genótipos de melancia

Detalhes bibliográficos
Autor(a) principal: Silva, Carla Maria de Jesus
Data de Publicação: 2018
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UEFS
Texto Completo: http://tede2.uefs.br:8080/handle/tede/tede/631
Resumo: The watermelon (Citrullus lanatus) is a very important vegetable for a region of Northeast Brazil due to its adaptation as the natural conditions and the good characteristics of the fruit that are obtained. The aims of the current study are to assess watermelon genotype responses to calluses in anthers by using growth and temperature regulators, and to induce polyploidy through colchicine use (at different concentrations), exposure time, mechanical scarification and application methods. Anthers of Smile and Sugar Baby lines were inoculated in MS medium at different concentrations of 2.4-D or of BAP with 2.4-D, in combination with the pre-treatment (low temperature). Crimson Sweet cultivar seedlings were treated with different colchicine concentrations at two different times, with and without scarification, in order to induce polyploidy. Line LDRO was subjected to different colchicine concentrations at two different times and application methods: a) direct in the seed method (with, and without, scarification) (DSM, WE and WOE), b) Radicle emission method (ERM), c) Hypocotyl and root insertion point method (HRIM), d) At the apex of the seedling method (ASM) and e) Inverted hypocotyl method (IHM). Crimson Sweet flower buds were subjected to colchicine, at two different times, in order to induce polyploidy. Results have shown that 2.4-D often induced callus formation in both lines, but BAP/2.4-D interaction and pre-treatment did not increase the induction frequency. Crimson Sweet showed higher induction rate at 0.2% colchicine for 48h, WE. Line LDRO presented plants with tetraploid cells at 0.2% colchicine for 24 and 48h. The method 0.2% DSM, WE and WOE also generated plants with tetraploid cells. The diameter of treated pollen grains in flower buds have increased; the higher rate of non-reduced gametes induction was 16.07% and flower bud diameter (1.5mm) was estimated as adequate for induction
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spelling Melo, Natoniel Franklin de41889665487http://lattes.cnpq.br/0592333655980257Dias, Rita de Cássia Souza43550282400http://lattes.cnpq.br/211993474876827803041227428http://lattes.cnpq.br/0101542800804313Silva, Carla Maria de Jesus2018-07-03T23:23:34Z2018-03-29SILVA, Carla Maria de Jesus. Indução de calos em anteras e poliploidia em genótipos de melancia. 2018. 96f. Tese (Doutorado Acadêmico em Recursos Genéticos Vegetais) - Universidade Estadual de Feira de Santana, Feira de Santana, 2018.http://tede2.uefs.br:8080/handle/tede/tede/631The watermelon (Citrullus lanatus) is a very important vegetable for a region of Northeast Brazil due to its adaptation as the natural conditions and the good characteristics of the fruit that are obtained. The aims of the current study are to assess watermelon genotype responses to calluses in anthers by using growth and temperature regulators, and to induce polyploidy through colchicine use (at different concentrations), exposure time, mechanical scarification and application methods. Anthers of Smile and Sugar Baby lines were inoculated in MS medium at different concentrations of 2.4-D or of BAP with 2.4-D, in combination with the pre-treatment (low temperature). Crimson Sweet cultivar seedlings were treated with different colchicine concentrations at two different times, with and without scarification, in order to induce polyploidy. Line LDRO was subjected to different colchicine concentrations at two different times and application methods: a) direct in the seed method (with, and without, scarification) (DSM, WE and WOE), b) Radicle emission method (ERM), c) Hypocotyl and root insertion point method (HRIM), d) At the apex of the seedling method (ASM) and e) Inverted hypocotyl method (IHM). Crimson Sweet flower buds were subjected to colchicine, at two different times, in order to induce polyploidy. Results have shown that 2.4-D often induced callus formation in both lines, but BAP/2.4-D interaction and pre-treatment did not increase the induction frequency. Crimson Sweet showed higher induction rate at 0.2% colchicine for 48h, WE. Line LDRO presented plants with tetraploid cells at 0.2% colchicine for 24 and 48h. The method 0.2% DSM, WE and WOE also generated plants with tetraploid cells. The diameter of treated pollen grains in flower buds have increased; the higher rate of non-reduced gametes induction was 16.07% and flower bud diameter (1.5mm) was estimated as adequate for inductionA melancia (Citrullus lanatus) é uma hortaliça muito importante para a região Nordeste do Brasil por sua adaptação as condições climáticas e pelas boas características de fruto que se obtém. Os objetivos desse trabalho foram avaliar as respostas de genótipos de melancia quanto à indução de calos em anteras utilizando reguladores de crescimento e temperatura; e induzir a poliploidia mediante o uso da colchicina em diferentes concentrações, tempos de exposição, escarificação mecânica e métodos de aplicação. Anteras das linhagens de Smile e Sugar Baby foram inoculadas em meio MS sob diferentes concentrações de 2,4-D ou BAP com 2,4-D, associado ao pré-tratamento (baixa temperatura). Para indução de poliploidia, sementes da cultivar Crimson Sweet foram tratadas com diferentes concentrações de colchicina, em dois tempos, com e sem escarificação. Para a linhagem LDRO, utilizaram-se diferentes concentrações de colchicina em dois tempos e métodos de aplicação: a) Método direto na semente (com escarificação e sem escarificação) (MDS, CE e SE), b) Método da semente com emissão da radícula (MER), c) Método no ponto de inserção do hipocótilo e raiz (MIHR), d) Método no ápice da plântula (MAP) e e) Método do hipocótilo invertido (MHI). Na indução de poliploidia em botões florais de Crimson Sweet utilizou-se colchicina em dois tempos. Os resultados mostraram que o 2,4-D induziu a maior frequência de calos nas duas linhagens, a interação BAP com 2,4-D e o pré-tratamento, não aumentaram a frequência de indução. Para Crimson Sweet, o maior percentual de indução foi obtido com colchicina a 0,2% por 48 h, SE. Para LDRO, observou-se uma frequência de plantas com células tetraploides com colchicina a 0,2% por 24 h e 48 h. Nos métodos 0,2% MDS CE e MHI observou-se também plantas com células tetraploides. Em botões florais, o diâmetro dos grãos de pólen tratados aumentou; o maior percentual de indução de gametas não reduzidos foi de 16,07% e; o diâmetro em torno de 1,5 mm do botão floral foi estimado como adequado para induçãoSubmitted by Verena Pereira (verenagoncalves@uefs.br) on 2018-07-03T23:23:34Z No. of bitstreams: 1 TESE 2018 - Doutorado RGV_UEFS - Carla Maria de Jesus Silva.pdf: 1687907 bytes, checksum: 3e9fe14e189c00f951079ca2a71e3c66 (MD5)Made available in DSpace on 2018-07-03T23:23:34Z (GMT). No. of bitstreams: 1 TESE 2018 - Doutorado RGV_UEFS - Carla Maria de Jesus Silva.pdf: 1687907 bytes, checksum: 3e9fe14e189c00f951079ca2a71e3c66 (MD5) Previous issue date: 2018-03-29Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Estadual de Feira de SantanaDoutorado Acadêmico em Recursos Genéticos VegetaisUEFSBrasilDEPARTAMENTO DE CIÊNCIAS BIOLÓGICASCalogênese em anterasCitogenéticaCitrullus lanatusGametas não reduzidosMelancia sem sementeViabilidade polínica em melanciaCalogenesis in anthersCytogeneticsCitrullus lanatusUnreduced gametesSeedless watermelonPollen viability in watermelonGENETICA::GENETICA VEGETALIndução de calos em anteras e poliploidia em genótipos de melanciainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis2074743607827725103600600600600-6971480722008537872-73979202484192807162075167498588264571info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEFSinstname:Universidade Estadual de Feira de Santana (UEFS)instacron:UEFSORIGINALTESE 2018 - Doutorado RGV_UEFS - Carla Maria de Jesus Silva.pdfTESE 2018 - Doutorado RGV_UEFS - Carla Maria de Jesus Silva.pdfapplication/pdf1687907http://tede2.uefs.br:8080/bitstream/tede/631/2/TESE+2018+-+Doutorado+RGV_UEFS+-+Carla+Maria+de+Jesus+Silva.pdf3e9fe14e189c00f951079ca2a71e3c66MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://tede2.uefs.br:8080/bitstream/tede/631/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/6312018-07-03 20:23:34.802oai:tede2.uefs.br:8080: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.uefs.br:8080/PUBhttp://tede2.uefs.br:8080/oai/requestbcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.bropendoar:2018-07-03T23:23:34Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS)false
dc.title.por.fl_str_mv Indução de calos em anteras e poliploidia em genótipos de melancia
title Indução de calos em anteras e poliploidia em genótipos de melancia
spellingShingle Indução de calos em anteras e poliploidia em genótipos de melancia
Silva, Carla Maria de Jesus
Calogênese em anteras
Citogenética
Citrullus lanatus
Gametas não reduzidos
Melancia sem semente
Viabilidade polínica em melancia
Calogenesis in anthers
Cytogenetics
Citrullus lanatus
Unreduced gametes
Seedless watermelon
Pollen viability in watermelon
GENETICA::GENETICA VEGETAL
title_short Indução de calos em anteras e poliploidia em genótipos de melancia
title_full Indução de calos em anteras e poliploidia em genótipos de melancia
title_fullStr Indução de calos em anteras e poliploidia em genótipos de melancia
title_full_unstemmed Indução de calos em anteras e poliploidia em genótipos de melancia
title_sort Indução de calos em anteras e poliploidia em genótipos de melancia
author Silva, Carla Maria de Jesus
author_facet Silva, Carla Maria de Jesus
author_role author
dc.contributor.advisor1.fl_str_mv Melo, Natoniel Franklin de
dc.contributor.advisor1ID.fl_str_mv 41889665487
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/0592333655980257
dc.contributor.advisor-co1.fl_str_mv Dias, Rita de Cássia Souza
dc.contributor.advisor-co1ID.fl_str_mv 43550282400
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/2119934748768278
dc.contributor.authorID.fl_str_mv 03041227428
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/0101542800804313
dc.contributor.author.fl_str_mv Silva, Carla Maria de Jesus
contributor_str_mv Melo, Natoniel Franklin de
Dias, Rita de Cássia Souza
dc.subject.por.fl_str_mv Calogênese em anteras
Citogenética
Citrullus lanatus
Gametas não reduzidos
Melancia sem semente
Viabilidade polínica em melancia
topic Calogênese em anteras
Citogenética
Citrullus lanatus
Gametas não reduzidos
Melancia sem semente
Viabilidade polínica em melancia
Calogenesis in anthers
Cytogenetics
Citrullus lanatus
Unreduced gametes
Seedless watermelon
Pollen viability in watermelon
GENETICA::GENETICA VEGETAL
dc.subject.eng.fl_str_mv Calogenesis in anthers
Cytogenetics
Citrullus lanatus
Unreduced gametes
Seedless watermelon
Pollen viability in watermelon
dc.subject.cnpq.fl_str_mv GENETICA::GENETICA VEGETAL
description The watermelon (Citrullus lanatus) is a very important vegetable for a region of Northeast Brazil due to its adaptation as the natural conditions and the good characteristics of the fruit that are obtained. The aims of the current study are to assess watermelon genotype responses to calluses in anthers by using growth and temperature regulators, and to induce polyploidy through colchicine use (at different concentrations), exposure time, mechanical scarification and application methods. Anthers of Smile and Sugar Baby lines were inoculated in MS medium at different concentrations of 2.4-D or of BAP with 2.4-D, in combination with the pre-treatment (low temperature). Crimson Sweet cultivar seedlings were treated with different colchicine concentrations at two different times, with and without scarification, in order to induce polyploidy. Line LDRO was subjected to different colchicine concentrations at two different times and application methods: a) direct in the seed method (with, and without, scarification) (DSM, WE and WOE), b) Radicle emission method (ERM), c) Hypocotyl and root insertion point method (HRIM), d) At the apex of the seedling method (ASM) and e) Inverted hypocotyl method (IHM). Crimson Sweet flower buds were subjected to colchicine, at two different times, in order to induce polyploidy. Results have shown that 2.4-D often induced callus formation in both lines, but BAP/2.4-D interaction and pre-treatment did not increase the induction frequency. Crimson Sweet showed higher induction rate at 0.2% colchicine for 48h, WE. Line LDRO presented plants with tetraploid cells at 0.2% colchicine for 24 and 48h. The method 0.2% DSM, WE and WOE also generated plants with tetraploid cells. The diameter of treated pollen grains in flower buds have increased; the higher rate of non-reduced gametes induction was 16.07% and flower bud diameter (1.5mm) was estimated as adequate for induction
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-07-03T23:23:34Z
dc.date.issued.fl_str_mv 2018-03-29
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
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dc.identifier.citation.fl_str_mv SILVA, Carla Maria de Jesus. Indução de calos em anteras e poliploidia em genótipos de melancia. 2018. 96f. Tese (Doutorado Acadêmico em Recursos Genéticos Vegetais) - Universidade Estadual de Feira de Santana, Feira de Santana, 2018.
dc.identifier.uri.fl_str_mv http://tede2.uefs.br:8080/handle/tede/tede/631
identifier_str_mv SILVA, Carla Maria de Jesus. Indução de calos em anteras e poliploidia em genótipos de melancia. 2018. 96f. Tese (Doutorado Acadêmico em Recursos Genéticos Vegetais) - Universidade Estadual de Feira de Santana, Feira de Santana, 2018.
url http://tede2.uefs.br:8080/handle/tede/tede/631
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language por
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dc.publisher.none.fl_str_mv Universidade Estadual de Feira de Santana
dc.publisher.program.fl_str_mv Doutorado Acadêmico em Recursos Genéticos Vegetais
dc.publisher.initials.fl_str_mv UEFS
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv DEPARTAMENTO DE CIÊNCIAS BIOLÓGICAS
publisher.none.fl_str_mv Universidade Estadual de Feira de Santana
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