Indução de calos em anteras e poliploidia em genótipos de melancia
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UEFS |
Texto Completo: | http://tede2.uefs.br:8080/handle/tede/tede/631 |
Resumo: | The watermelon (Citrullus lanatus) is a very important vegetable for a region of Northeast Brazil due to its adaptation as the natural conditions and the good characteristics of the fruit that are obtained. The aims of the current study are to assess watermelon genotype responses to calluses in anthers by using growth and temperature regulators, and to induce polyploidy through colchicine use (at different concentrations), exposure time, mechanical scarification and application methods. Anthers of Smile and Sugar Baby lines were inoculated in MS medium at different concentrations of 2.4-D or of BAP with 2.4-D, in combination with the pre-treatment (low temperature). Crimson Sweet cultivar seedlings were treated with different colchicine concentrations at two different times, with and without scarification, in order to induce polyploidy. Line LDRO was subjected to different colchicine concentrations at two different times and application methods: a) direct in the seed method (with, and without, scarification) (DSM, WE and WOE), b) Radicle emission method (ERM), c) Hypocotyl and root insertion point method (HRIM), d) At the apex of the seedling method (ASM) and e) Inverted hypocotyl method (IHM). Crimson Sweet flower buds were subjected to colchicine, at two different times, in order to induce polyploidy. Results have shown that 2.4-D often induced callus formation in both lines, but BAP/2.4-D interaction and pre-treatment did not increase the induction frequency. Crimson Sweet showed higher induction rate at 0.2% colchicine for 48h, WE. Line LDRO presented plants with tetraploid cells at 0.2% colchicine for 24 and 48h. The method 0.2% DSM, WE and WOE also generated plants with tetraploid cells. The diameter of treated pollen grains in flower buds have increased; the higher rate of non-reduced gametes induction was 16.07% and flower bud diameter (1.5mm) was estimated as adequate for induction |
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Melo, Natoniel Franklin de41889665487http://lattes.cnpq.br/0592333655980257Dias, Rita de Cássia Souza43550282400http://lattes.cnpq.br/211993474876827803041227428http://lattes.cnpq.br/0101542800804313Silva, Carla Maria de Jesus2018-07-03T23:23:34Z2018-03-29SILVA, Carla Maria de Jesus. Indução de calos em anteras e poliploidia em genótipos de melancia. 2018. 96f. Tese (Doutorado Acadêmico em Recursos Genéticos Vegetais) - Universidade Estadual de Feira de Santana, Feira de Santana, 2018.http://tede2.uefs.br:8080/handle/tede/tede/631The watermelon (Citrullus lanatus) is a very important vegetable for a region of Northeast Brazil due to its adaptation as the natural conditions and the good characteristics of the fruit that are obtained. The aims of the current study are to assess watermelon genotype responses to calluses in anthers by using growth and temperature regulators, and to induce polyploidy through colchicine use (at different concentrations), exposure time, mechanical scarification and application methods. Anthers of Smile and Sugar Baby lines were inoculated in MS medium at different concentrations of 2.4-D or of BAP with 2.4-D, in combination with the pre-treatment (low temperature). Crimson Sweet cultivar seedlings were treated with different colchicine concentrations at two different times, with and without scarification, in order to induce polyploidy. Line LDRO was subjected to different colchicine concentrations at two different times and application methods: a) direct in the seed method (with, and without, scarification) (DSM, WE and WOE), b) Radicle emission method (ERM), c) Hypocotyl and root insertion point method (HRIM), d) At the apex of the seedling method (ASM) and e) Inverted hypocotyl method (IHM). Crimson Sweet flower buds were subjected to colchicine, at two different times, in order to induce polyploidy. Results have shown that 2.4-D often induced callus formation in both lines, but BAP/2.4-D interaction and pre-treatment did not increase the induction frequency. Crimson Sweet showed higher induction rate at 0.2% colchicine for 48h, WE. Line LDRO presented plants with tetraploid cells at 0.2% colchicine for 24 and 48h. The method 0.2% DSM, WE and WOE also generated plants with tetraploid cells. The diameter of treated pollen grains in flower buds have increased; the higher rate of non-reduced gametes induction was 16.07% and flower bud diameter (1.5mm) was estimated as adequate for inductionA melancia (Citrullus lanatus) é uma hortaliça muito importante para a região Nordeste do Brasil por sua adaptação as condições climáticas e pelas boas características de fruto que se obtém. Os objetivos desse trabalho foram avaliar as respostas de genótipos de melancia quanto à indução de calos em anteras utilizando reguladores de crescimento e temperatura; e induzir a poliploidia mediante o uso da colchicina em diferentes concentrações, tempos de exposição, escarificação mecânica e métodos de aplicação. Anteras das linhagens de Smile e Sugar Baby foram inoculadas em meio MS sob diferentes concentrações de 2,4-D ou BAP com 2,4-D, associado ao pré-tratamento (baixa temperatura). Para indução de poliploidia, sementes da cultivar Crimson Sweet foram tratadas com diferentes concentrações de colchicina, em dois tempos, com e sem escarificação. Para a linhagem LDRO, utilizaram-se diferentes concentrações de colchicina em dois tempos e métodos de aplicação: a) Método direto na semente (com escarificação e sem escarificação) (MDS, CE e SE), b) Método da semente com emissão da radícula (MER), c) Método no ponto de inserção do hipocótilo e raiz (MIHR), d) Método no ápice da plântula (MAP) e e) Método do hipocótilo invertido (MHI). Na indução de poliploidia em botões florais de Crimson Sweet utilizou-se colchicina em dois tempos. Os resultados mostraram que o 2,4-D induziu a maior frequência de calos nas duas linhagens, a interação BAP com 2,4-D e o pré-tratamento, não aumentaram a frequência de indução. Para Crimson Sweet, o maior percentual de indução foi obtido com colchicina a 0,2% por 48 h, SE. Para LDRO, observou-se uma frequência de plantas com células tetraploides com colchicina a 0,2% por 24 h e 48 h. Nos métodos 0,2% MDS CE e MHI observou-se também plantas com células tetraploides. Em botões florais, o diâmetro dos grãos de pólen tratados aumentou; o maior percentual de indução de gametas não reduzidos foi de 16,07% e; o diâmetro em torno de 1,5 mm do botão floral foi estimado como adequado para induçãoSubmitted by Verena Pereira (verenagoncalves@uefs.br) on 2018-07-03T23:23:34Z No. of bitstreams: 1 TESE 2018 - Doutorado RGV_UEFS - Carla Maria de Jesus Silva.pdf: 1687907 bytes, checksum: 3e9fe14e189c00f951079ca2a71e3c66 (MD5)Made available in DSpace on 2018-07-03T23:23:34Z (GMT). No. of bitstreams: 1 TESE 2018 - Doutorado RGV_UEFS - Carla Maria de Jesus Silva.pdf: 1687907 bytes, checksum: 3e9fe14e189c00f951079ca2a71e3c66 (MD5) Previous issue date: 2018-03-29Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Estadual de Feira de SantanaDoutorado Acadêmico em Recursos Genéticos VegetaisUEFSBrasilDEPARTAMENTO DE CIÊNCIAS BIOLÓGICASCalogênese em anterasCitogenéticaCitrullus lanatusGametas não reduzidosMelancia sem sementeViabilidade polínica em melanciaCalogenesis in anthersCytogeneticsCitrullus lanatusUnreduced gametesSeedless watermelonPollen viability in watermelonGENETICA::GENETICA VEGETALIndução de calos em anteras e poliploidia em genótipos de melanciainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis2074743607827725103600600600600-6971480722008537872-73979202484192807162075167498588264571info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEFSinstname:Universidade Estadual de Feira de Santana (UEFS)instacron:UEFSORIGINALTESE 2018 - Doutorado RGV_UEFS - Carla Maria de Jesus Silva.pdfTESE 2018 - Doutorado RGV_UEFS - Carla Maria de Jesus Silva.pdfapplication/pdf1687907http://tede2.uefs.br:8080/bitstream/tede/631/2/TESE+2018+-+Doutorado+RGV_UEFS+-+Carla+Maria+de+Jesus+Silva.pdf3e9fe14e189c00f951079ca2a71e3c66MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://tede2.uefs.br:8080/bitstream/tede/631/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/6312018-07-03 20:23:34.802oai:tede2.uefs.br:8080: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.uefs.br:8080/PUBhttp://tede2.uefs.br:8080/oai/requestbcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.bropendoar:2018-07-03T23:23:34Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS)false |
dc.title.por.fl_str_mv |
Indução de calos em anteras e poliploidia em genótipos de melancia |
title |
Indução de calos em anteras e poliploidia em genótipos de melancia |
spellingShingle |
Indução de calos em anteras e poliploidia em genótipos de melancia Silva, Carla Maria de Jesus Calogênese em anteras Citogenética Citrullus lanatus Gametas não reduzidos Melancia sem semente Viabilidade polínica em melancia Calogenesis in anthers Cytogenetics Citrullus lanatus Unreduced gametes Seedless watermelon Pollen viability in watermelon GENETICA::GENETICA VEGETAL |
title_short |
Indução de calos em anteras e poliploidia em genótipos de melancia |
title_full |
Indução de calos em anteras e poliploidia em genótipos de melancia |
title_fullStr |
Indução de calos em anteras e poliploidia em genótipos de melancia |
title_full_unstemmed |
Indução de calos em anteras e poliploidia em genótipos de melancia |
title_sort |
Indução de calos em anteras e poliploidia em genótipos de melancia |
author |
Silva, Carla Maria de Jesus |
author_facet |
Silva, Carla Maria de Jesus |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Melo, Natoniel Franklin de |
dc.contributor.advisor1ID.fl_str_mv |
41889665487 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/0592333655980257 |
dc.contributor.advisor-co1.fl_str_mv |
Dias, Rita de Cássia Souza |
dc.contributor.advisor-co1ID.fl_str_mv |
43550282400 |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/2119934748768278 |
dc.contributor.authorID.fl_str_mv |
03041227428 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0101542800804313 |
dc.contributor.author.fl_str_mv |
Silva, Carla Maria de Jesus |
contributor_str_mv |
Melo, Natoniel Franklin de Dias, Rita de Cássia Souza |
dc.subject.por.fl_str_mv |
Calogênese em anteras Citogenética Citrullus lanatus Gametas não reduzidos Melancia sem semente Viabilidade polínica em melancia |
topic |
Calogênese em anteras Citogenética Citrullus lanatus Gametas não reduzidos Melancia sem semente Viabilidade polínica em melancia Calogenesis in anthers Cytogenetics Citrullus lanatus Unreduced gametes Seedless watermelon Pollen viability in watermelon GENETICA::GENETICA VEGETAL |
dc.subject.eng.fl_str_mv |
Calogenesis in anthers Cytogenetics Citrullus lanatus Unreduced gametes Seedless watermelon Pollen viability in watermelon |
dc.subject.cnpq.fl_str_mv |
GENETICA::GENETICA VEGETAL |
description |
The watermelon (Citrullus lanatus) is a very important vegetable for a region of Northeast Brazil due to its adaptation as the natural conditions and the good characteristics of the fruit that are obtained. The aims of the current study are to assess watermelon genotype responses to calluses in anthers by using growth and temperature regulators, and to induce polyploidy through colchicine use (at different concentrations), exposure time, mechanical scarification and application methods. Anthers of Smile and Sugar Baby lines were inoculated in MS medium at different concentrations of 2.4-D or of BAP with 2.4-D, in combination with the pre-treatment (low temperature). Crimson Sweet cultivar seedlings were treated with different colchicine concentrations at two different times, with and without scarification, in order to induce polyploidy. Line LDRO was subjected to different colchicine concentrations at two different times and application methods: a) direct in the seed method (with, and without, scarification) (DSM, WE and WOE), b) Radicle emission method (ERM), c) Hypocotyl and root insertion point method (HRIM), d) At the apex of the seedling method (ASM) and e) Inverted hypocotyl method (IHM). Crimson Sweet flower buds were subjected to colchicine, at two different times, in order to induce polyploidy. Results have shown that 2.4-D often induced callus formation in both lines, but BAP/2.4-D interaction and pre-treatment did not increase the induction frequency. Crimson Sweet showed higher induction rate at 0.2% colchicine for 48h, WE. Line LDRO presented plants with tetraploid cells at 0.2% colchicine for 24 and 48h. The method 0.2% DSM, WE and WOE also generated plants with tetraploid cells. The diameter of treated pollen grains in flower buds have increased; the higher rate of non-reduced gametes induction was 16.07% and flower bud diameter (1.5mm) was estimated as adequate for induction |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-07-03T23:23:34Z |
dc.date.issued.fl_str_mv |
2018-03-29 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, Carla Maria de Jesus. Indução de calos em anteras e poliploidia em genótipos de melancia. 2018. 96f. Tese (Doutorado Acadêmico em Recursos Genéticos Vegetais) - Universidade Estadual de Feira de Santana, Feira de Santana, 2018. |
dc.identifier.uri.fl_str_mv |
http://tede2.uefs.br:8080/handle/tede/tede/631 |
identifier_str_mv |
SILVA, Carla Maria de Jesus. Indução de calos em anteras e poliploidia em genótipos de melancia. 2018. 96f. Tese (Doutorado Acadêmico em Recursos Genéticos Vegetais) - Universidade Estadual de Feira de Santana, Feira de Santana, 2018. |
url |
http://tede2.uefs.br:8080/handle/tede/tede/631 |
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por |
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por |
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2074743607827725103 |
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600 600 600 600 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidade Estadual de Feira de Santana |
dc.publisher.program.fl_str_mv |
Doutorado Acadêmico em Recursos Genéticos Vegetais |
dc.publisher.initials.fl_str_mv |
UEFS |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
DEPARTAMENTO DE CIÊNCIAS BIOLÓGICAS |
publisher.none.fl_str_mv |
Universidade Estadual de Feira de Santana |
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