Transplante autólogo de células-tronco de medula óssea em pacientes com trauma raquimedular
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UEFS |
Texto Completo: | http://localhost:8080/tede/handle/tede/84 |
Resumo: | Despite the advancement in the care of patients with spinal cord injury, currently there is no specific treatment for neurological lesion. Therapy with mesenchymal stem cells from bone marrow (BMMC) has shown to be a safe and promising technique in various medical fields. The objective of this study is to evaluate the safety of autologous BMMC transplantation for the treatment of chronic spinal cord injury of traumatic origin.Fourteen paraplegic patients (classified as A in ASIA scale) with at least 6-month thoracic or thoracolumbar spinal cord injurywere selected. They underwent aspiration of bone marrow tissue from the iliac crest with further expansion and in vitro characterization; by means of microsurgical procedure, the BMMC suspension was introduced at the level of the spinal cord injury. A 6-month clinical follow-up was performed after surgery, including functional and imaging tests. There was a change in ASIA scale in 7 patients, increased bladder capacity in 5 patients and appearance of the SSEP waveform in one patient. Throughout the observation period, no major complications were observed. The present protocol proved to be safe and, due to significant improvements in neurological symptoms in many patients, also a potentially promising therapeutic method for patients with spinal cord injury. |
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Soares, Milena Botelho Pereira01249363750http://lattes.cnpq.br/5484905006036882Mendonça, Marcus Vinícius Pinheiro2015-07-24T01:11:42Z2014-10-10MENDONÇA, Marcus Vinícius Pinheiro. Transplante autólogo de células-tronco de medula óssea em pacientes com trauma raquimedular. 2014. 105 f. Tese (Doutorado Acadêmico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2014.http://localhost:8080/tede/handle/tede/84Despite the advancement in the care of patients with spinal cord injury, currently there is no specific treatment for neurological lesion. Therapy with mesenchymal stem cells from bone marrow (BMMC) has shown to be a safe and promising technique in various medical fields. The objective of this study is to evaluate the safety of autologous BMMC transplantation for the treatment of chronic spinal cord injury of traumatic origin.Fourteen paraplegic patients (classified as A in ASIA scale) with at least 6-month thoracic or thoracolumbar spinal cord injurywere selected. They underwent aspiration of bone marrow tissue from the iliac crest with further expansion and in vitro characterization; by means of microsurgical procedure, the BMMC suspension was introduced at the level of the spinal cord injury. A 6-month clinical follow-up was performed after surgery, including functional and imaging tests. There was a change in ASIA scale in 7 patients, increased bladder capacity in 5 patients and appearance of the SSEP waveform in one patient. Throughout the observation period, no major complications were observed. The present protocol proved to be safe and, due to significant improvements in neurological symptoms in many patients, also a potentially promising therapeutic method for patients with spinal cord injury.A despeito do avanço na assistência do paciente com trauma raquimedular, atualmente não há tratamento específico para a lesão neurológica. A terapia com células-tronco mesenquimais da medula óssea (CMMO) tem-se mostrado uma técnica segura e promissora em diversas áreas médicas. O objetivo deste estudo é avaliar a segurança do transplante autólogo de CMMO para o tratamento de lesão medular crônica de origem traumática. Foram selecionados 14 pacientes paraplégicos (classificados como A na escala ASIA) com lesão medular torácica ou tóraco-lombar há mais de 6 meses. Eles foram inicialmente submetidos à aspiração de tecido da medula óssea da crista ilíaca, com posterior expansão e caracterização in vitro de CMMO e, por meio de procedimento microcirúrgico, introduziu-se a suspensão de CMMO na medula espinhal no nível da lesão. Os pacientes foram acompanhados clinicamente, com realização de exames funcionais e de imagem por um período de 6 meses após o procedimento cirúrgico. Houve mudança na escala ASIA em 7 pacientes, aumento da capacidade vesical em 5 pacientes e aparecimento de onda ao SSEP em 1 paciente. Durante todo o tempo de observação, não foram identificadas complicações potencialmente graves. O presente protocolo se mostrou seguro e, ainda, devido a melhoras significativas no quadro neurológico em boa parte dos pacientes, um método terapêutico potencialmente promissor para pacientes com trauma raquimedular.Submitted by Natalie Mendes (nataliermendes@gmail.com) on 2015-07-24T01:11:42Z No. of bitstreams: 1 marcus_vinicius_pinheiro_mendonca_tese.pdf: 8890182 bytes, checksum: 8c3486b869dff02251529c805a28b23c (MD5)Made available in DSpace on 2015-07-24T01:11:42Z (GMT). No. of bitstreams: 1 marcus_vinicius_pinheiro_mendonca_tese.pdf: 8890182 bytes, checksum: 8c3486b869dff02251529c805a28b23c (MD5) Previous issue date: 2014-10-10application/pdfporUniversidade Estadual de Feira de SantanaDoutorado Acadêmico em BiotecnologiaUEFSBrasilDEPARTAMENTO DE SAÚDETrauma raquimedularCélulas-tronco mesenquimaisTransplanteSpinal cord injuryMesenchymal stem cellsTransplantCIENCIAS DA SAUDE::MEDICINATransplante autólogo de células-tronco de medula óssea em pacientes com trauma raquimedularinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis-55209245493529045686006006008549511740116106200-969369452308786627info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEFSinstname:Universidade Estadual de Feira de Santana (UEFS)instacron:UEFSORIGINALmarcus_vinicius_pinheiro_mendonca_tese.pdfmarcus_vinicius_pinheiro_mendonca_tese.pdfapplication/pdf8890182http://tede2.uefs.br:8080/bitstream/tede/84/2/marcus_vinicius_pinheiro_mendonca_tese.pdf8c3486b869dff02251529c805a28b23cMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://tede2.uefs.br:8080/bitstream/tede/84/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/842015-08-05 18:42:40.37oai:tede2.uefs.br:8080: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.uefs.br:8080/PUBhttp://tede2.uefs.br:8080/oai/requestbcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.bropendoar:2015-08-05T21:42:40Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS)false |
dc.title.por.fl_str_mv |
Transplante autólogo de células-tronco de medula óssea em pacientes com trauma raquimedular |
title |
Transplante autólogo de células-tronco de medula óssea em pacientes com trauma raquimedular |
spellingShingle |
Transplante autólogo de células-tronco de medula óssea em pacientes com trauma raquimedular Mendonça, Marcus Vinícius Pinheiro Trauma raquimedular Células-tronco mesenquimais Transplante Spinal cord injury Mesenchymal stem cells Transplant CIENCIAS DA SAUDE::MEDICINA |
title_short |
Transplante autólogo de células-tronco de medula óssea em pacientes com trauma raquimedular |
title_full |
Transplante autólogo de células-tronco de medula óssea em pacientes com trauma raquimedular |
title_fullStr |
Transplante autólogo de células-tronco de medula óssea em pacientes com trauma raquimedular |
title_full_unstemmed |
Transplante autólogo de células-tronco de medula óssea em pacientes com trauma raquimedular |
title_sort |
Transplante autólogo de células-tronco de medula óssea em pacientes com trauma raquimedular |
author |
Mendonça, Marcus Vinícius Pinheiro |
author_facet |
Mendonça, Marcus Vinícius Pinheiro |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Soares, Milena Botelho Pereira |
dc.contributor.authorID.fl_str_mv |
01249363750 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5484905006036882 |
dc.contributor.author.fl_str_mv |
Mendonça, Marcus Vinícius Pinheiro |
contributor_str_mv |
Soares, Milena Botelho Pereira |
dc.subject.por.fl_str_mv |
Trauma raquimedular Células-tronco mesenquimais Transplante |
topic |
Trauma raquimedular Células-tronco mesenquimais Transplante Spinal cord injury Mesenchymal stem cells Transplant CIENCIAS DA SAUDE::MEDICINA |
dc.subject.eng.fl_str_mv |
Spinal cord injury Mesenchymal stem cells Transplant |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::MEDICINA |
description |
Despite the advancement in the care of patients with spinal cord injury, currently there is no specific treatment for neurological lesion. Therapy with mesenchymal stem cells from bone marrow (BMMC) has shown to be a safe and promising technique in various medical fields. The objective of this study is to evaluate the safety of autologous BMMC transplantation for the treatment of chronic spinal cord injury of traumatic origin.Fourteen paraplegic patients (classified as A in ASIA scale) with at least 6-month thoracic or thoracolumbar spinal cord injurywere selected. They underwent aspiration of bone marrow tissue from the iliac crest with further expansion and in vitro characterization; by means of microsurgical procedure, the BMMC suspension was introduced at the level of the spinal cord injury. A 6-month clinical follow-up was performed after surgery, including functional and imaging tests. There was a change in ASIA scale in 7 patients, increased bladder capacity in 5 patients and appearance of the SSEP waveform in one patient. Throughout the observation period, no major complications were observed. The present protocol proved to be safe and, due to significant improvements in neurological symptoms in many patients, also a potentially promising therapeutic method for patients with spinal cord injury. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-10-10 |
dc.date.accessioned.fl_str_mv |
2015-07-24T01:11:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MENDONÇA, Marcus Vinícius Pinheiro. Transplante autólogo de células-tronco de medula óssea em pacientes com trauma raquimedular. 2014. 105 f. Tese (Doutorado Acadêmico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2014. |
dc.identifier.uri.fl_str_mv |
http://localhost:8080/tede/handle/tede/84 |
identifier_str_mv |
MENDONÇA, Marcus Vinícius Pinheiro. Transplante autólogo de células-tronco de medula óssea em pacientes com trauma raquimedular. 2014. 105 f. Tese (Doutorado Acadêmico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2014. |
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http://localhost:8080/tede/handle/tede/84 |
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por |
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por |
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600 600 600 |
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Universidade Estadual de Feira de Santana |
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Doutorado Acadêmico em Biotecnologia |
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UEFS |
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Universidade Estadual de Feira de Santana |
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