Aspectos morfoanatômicos e farmacognósticos de “gervão” Stachytarpheta jamaicensis (L.) Vahl (verbenaceae)
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UEFS |
Texto Completo: | http://localhost:8080/tede/handle/tede/337 |
Resumo: | The species was selected to carry out the research because of the wide medicinal use for diarrhea treatment by the Brazilian northeast population. The objective of this work was to study the morphological and anatomical aspects of the leaves of Stachytarpheta jamaicensis, detect the presence of the major classes of secondary metabolites present in the crude extract of the leaves and still assessing the potential antioxidant, cytotoxic and antibacterial said extract and its fractions. To achieve the objectives, paradermic sections and cross were made to study the epidermis and its annexes. Was also analyzed characters of leaf morphology and petiole through histological sections. Phytochemical screening of crude extracts was performed by colorimetric tests and tests precipitation. From the raw methanol extract was held partitions with increasing polarity solvents (hexane, chloroform and ethyl acetate). The fractionation and purification of the partitioned extracts was performed by column chromatography (CC) and monitored by thin layer chromatography (TLC). The crude extract (EB), and fractions were subjected to test the free radical sequestration 1,1 - diphenyl-2-picrylhydrazyl (DPPH) test and the mortality of Artemia salina, to assess antioxidant activities and cytotoxic activities, respectively. To evaluate the antibacterial activity were performed the disk diffusion techniques in well and MIC (minimum inhibitory concentration) compared to the strains of Salmonella and E.coli. The blade is amphistomatic with stomata of diacític type, the epidermis is uniseriate, with the presence of trichomes. The results detected the presence of tannins, flavonoids, alkaloids and saponins. In the evaluation of antioxidant activity, IC 50 values (mg / ml) were found 6.62 to 446.20 for the tested extracts. For cytotoxicity, all extracts showed LC50 values (mg / ml) 211.16 to 446.20, and the hexane extract the most active (LC50 211.16). For antibacterial analysis, has not been demonstrated activity of extracts front bacteria tested. The toxicity of the leaf of this species refers caution about their safety for use by the population. |
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Silva, Tânia Regina dos Santos02791619569http://lattes.cnpq.br/5502776449602089Almeida, Laís Cardoso2016-06-14T22:02:36Z2014-08-07ALMEIDA, Laís Cardoso. Aspectos morfoanatômicos e farmacognósticos de “gervão” Stachytarpheta jamaicensis (L.) Vahl (verbenaceae). 2014. 43 f. Dissertação (Mestrado Acadêmico em Recursos Genéticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2014.http://localhost:8080/tede/handle/tede/337The species was selected to carry out the research because of the wide medicinal use for diarrhea treatment by the Brazilian northeast population. The objective of this work was to study the morphological and anatomical aspects of the leaves of Stachytarpheta jamaicensis, detect the presence of the major classes of secondary metabolites present in the crude extract of the leaves and still assessing the potential antioxidant, cytotoxic and antibacterial said extract and its fractions. To achieve the objectives, paradermic sections and cross were made to study the epidermis and its annexes. Was also analyzed characters of leaf morphology and petiole through histological sections. Phytochemical screening of crude extracts was performed by colorimetric tests and tests precipitation. From the raw methanol extract was held partitions with increasing polarity solvents (hexane, chloroform and ethyl acetate). The fractionation and purification of the partitioned extracts was performed by column chromatography (CC) and monitored by thin layer chromatography (TLC). The crude extract (EB), and fractions were subjected to test the free radical sequestration 1,1 - diphenyl-2-picrylhydrazyl (DPPH) test and the mortality of Artemia salina, to assess antioxidant activities and cytotoxic activities, respectively. To evaluate the antibacterial activity were performed the disk diffusion techniques in well and MIC (minimum inhibitory concentration) compared to the strains of Salmonella and E.coli. The blade is amphistomatic with stomata of diacític type, the epidermis is uniseriate, with the presence of trichomes. The results detected the presence of tannins, flavonoids, alkaloids and saponins. In the evaluation of antioxidant activity, IC 50 values (mg / ml) were found 6.62 to 446.20 for the tested extracts. For cytotoxicity, all extracts showed LC50 values (mg / ml) 211.16 to 446.20, and the hexane extract the most active (LC50 211.16). For antibacterial analysis, has not been demonstrated activity of extracts front bacteria tested. The toxicity of the leaf of this species refers caution about their safety for use by the population.A espécie foi selecionada para realização da pesquisa devido à ampla utilização medicinal para tratamento de diarreia pela população do nordeste brasileiro. O objetivo desse trabalho foi estudar os aspectos morfoanâtomicos das folhas de Stachytarpheta jamaicensis, detectar presença das principais classes de metabólitos secundários presentes no extrato bruto de suas folhas e ainda avaliação do potencial antioxidante, citotóxico e antibacteriano do referido extrato e suas frações. Para alcançar os objetivos, secções paradérmicas e transversais foram efetuadas para estudo da epiderme e seus anexos. Analisou-se também caracteres da morfologia foliar e do pecíolo através de cortes histológicos. Foi realizada triagem fitoquímica do extrato bruto através de testes colorimétricos e ensaios de precipitação. A partir do extrato bruto metanólico, realizou-se partições com solventes de polaridade crescente (hexano, clorofórmio e acetato de etila). O fracionamento e purificação dos extratos particionados foi realizado por cromatografia em coluna (CC) e monitorado por cromatografia em camada delgada (CCD). O Extrato Bruto (EB) e as fracões foram submetidos ao teste do sequestro do radical livre 1,1 – difenil-2-picrilhidrazil (DPPH) e ao ensaio de letalidade da Artemia salina, para avaliação das atividades antioxidante e citotóxica, respectivamente. Para avaliação da atividade antibacteriana foram executadas as técnicas de difusão em disco, em poço e MIC (concentração inibitória mínima) frente às cepas de Samonella e E.coli. A lâmina foliar é anfiestomática, com estômatos do tipo diacítico, a epiderme é uniestratificada, com presença de tricomas tectores. Os resultados detectaram presença de taninos, flavonoides, alcaloides e saponinas. Na avaliação da atividade antioxidante, os valores de IC50 (mg/ml) encontrados foram de 6,62 a 446,20 para os extratos testados. Quanto à citotoxicidade, todos os extratos apresentaram valores de LC50 (mg/ml) de 211,16 a 446,20, sendo o extrato hexânico o mais ativo (LC50 211,16) . Quanto à análise antibacteriana, não foi demonstrada atividade dos extratos frente as bactérias testadas. A toxicidade apresentada pela folha dessa espécie remete cautela quanto à sua segurança para uso pela população.Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2016-06-14T22:02:36Z No. of bitstreams: 1 63-lais-cardoso-dissertacao-completa-07-08-2014.pdf: 1594778 bytes, checksum: 095a7789d1a934007d9907b518ed7989 (MD5)Made available in DSpace on 2016-06-14T22:02:36Z (GMT). No. of bitstreams: 1 63-lais-cardoso-dissertacao-completa-07-08-2014.pdf: 1594778 bytes, checksum: 095a7789d1a934007d9907b518ed7989 (MD5) Previous issue date: 2014-08-07Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Estadual de Feira de SantanaMestrado Acadêmico em Recursos Genéticos VegetaisUEFSBrasilDEPARTAMENTO DE CIÊNCIAS BIOLÓGICASStachytarpheta jamaicensisMorfoanatomiaFitoquímicaAtividade biológicaCitotoxidadeMorphologyAnatomyPhytochemistryBiological activityCytotoxicityStachytarpheta jamaicensisCIENCIAS BIOLOGICAS::BIOLOGIA GERALAspectos morfoanatômicos e farmacognósticos de “gervão” Stachytarpheta jamaicensis (L.) 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dc.title.por.fl_str_mv |
Aspectos morfoanatômicos e farmacognósticos de “gervão” Stachytarpheta jamaicensis (L.) Vahl (verbenaceae) |
title |
Aspectos morfoanatômicos e farmacognósticos de “gervão” Stachytarpheta jamaicensis (L.) Vahl (verbenaceae) |
spellingShingle |
Aspectos morfoanatômicos e farmacognósticos de “gervão” Stachytarpheta jamaicensis (L.) Vahl (verbenaceae) Almeida, Laís Cardoso Stachytarpheta jamaicensis Morfoanatomia Fitoquímica Atividade biológica Citotoxidade Morphology Anatomy Phytochemistry Biological activity Cytotoxicity Stachytarpheta jamaicensis CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
title_short |
Aspectos morfoanatômicos e farmacognósticos de “gervão” Stachytarpheta jamaicensis (L.) Vahl (verbenaceae) |
title_full |
Aspectos morfoanatômicos e farmacognósticos de “gervão” Stachytarpheta jamaicensis (L.) Vahl (verbenaceae) |
title_fullStr |
Aspectos morfoanatômicos e farmacognósticos de “gervão” Stachytarpheta jamaicensis (L.) Vahl (verbenaceae) |
title_full_unstemmed |
Aspectos morfoanatômicos e farmacognósticos de “gervão” Stachytarpheta jamaicensis (L.) Vahl (verbenaceae) |
title_sort |
Aspectos morfoanatômicos e farmacognósticos de “gervão” Stachytarpheta jamaicensis (L.) Vahl (verbenaceae) |
author |
Almeida, Laís Cardoso |
author_facet |
Almeida, Laís Cardoso |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Silva, Tânia Regina dos Santos |
dc.contributor.authorID.fl_str_mv |
02791619569 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5502776449602089 |
dc.contributor.author.fl_str_mv |
Almeida, Laís Cardoso |
contributor_str_mv |
Silva, Tânia Regina dos Santos |
dc.subject.por.fl_str_mv |
Stachytarpheta jamaicensis Morfoanatomia Fitoquímica Atividade biológica Citotoxidade |
topic |
Stachytarpheta jamaicensis Morfoanatomia Fitoquímica Atividade biológica Citotoxidade Morphology Anatomy Phytochemistry Biological activity Cytotoxicity Stachytarpheta jamaicensis CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
dc.subject.eng.fl_str_mv |
Morphology Anatomy Phytochemistry Biological activity Cytotoxicity Stachytarpheta jamaicensis |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
description |
The species was selected to carry out the research because of the wide medicinal use for diarrhea treatment by the Brazilian northeast population. The objective of this work was to study the morphological and anatomical aspects of the leaves of Stachytarpheta jamaicensis, detect the presence of the major classes of secondary metabolites present in the crude extract of the leaves and still assessing the potential antioxidant, cytotoxic and antibacterial said extract and its fractions. To achieve the objectives, paradermic sections and cross were made to study the epidermis and its annexes. Was also analyzed characters of leaf morphology and petiole through histological sections. Phytochemical screening of crude extracts was performed by colorimetric tests and tests precipitation. From the raw methanol extract was held partitions with increasing polarity solvents (hexane, chloroform and ethyl acetate). The fractionation and purification of the partitioned extracts was performed by column chromatography (CC) and monitored by thin layer chromatography (TLC). The crude extract (EB), and fractions were subjected to test the free radical sequestration 1,1 - diphenyl-2-picrylhydrazyl (DPPH) test and the mortality of Artemia salina, to assess antioxidant activities and cytotoxic activities, respectively. To evaluate the antibacterial activity were performed the disk diffusion techniques in well and MIC (minimum inhibitory concentration) compared to the strains of Salmonella and E.coli. The blade is amphistomatic with stomata of diacític type, the epidermis is uniseriate, with the presence of trichomes. The results detected the presence of tannins, flavonoids, alkaloids and saponins. In the evaluation of antioxidant activity, IC 50 values (mg / ml) were found 6.62 to 446.20 for the tested extracts. For cytotoxicity, all extracts showed LC50 values (mg / ml) 211.16 to 446.20, and the hexane extract the most active (LC50 211.16). For antibacterial analysis, has not been demonstrated activity of extracts front bacteria tested. The toxicity of the leaf of this species refers caution about their safety for use by the population. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-08-07 |
dc.date.accessioned.fl_str_mv |
2016-06-14T22:02:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ALMEIDA, Laís Cardoso. Aspectos morfoanatômicos e farmacognósticos de “gervão” Stachytarpheta jamaicensis (L.) Vahl (verbenaceae). 2014. 43 f. Dissertação (Mestrado Acadêmico em Recursos Genéticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2014. |
dc.identifier.uri.fl_str_mv |
http://localhost:8080/tede/handle/tede/337 |
identifier_str_mv |
ALMEIDA, Laís Cardoso. Aspectos morfoanatômicos e farmacognósticos de “gervão” Stachytarpheta jamaicensis (L.) Vahl (verbenaceae). 2014. 43 f. Dissertação (Mestrado Acadêmico em Recursos Genéticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2014. |
url |
http://localhost:8080/tede/handle/tede/337 |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Estadual de Feira de Santana |
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dc.publisher.initials.fl_str_mv |
UEFS |
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Brasil |
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DEPARTAMENTO DE CIÊNCIAS BIOLÓGICAS |
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Universidade Estadual de Feira de Santana |
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