Estudos de inibição e modelagem por homologia da enzima beta(1,3)-d-glicano sintase de moniliophthora perniciosa
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UEFS |
Texto Completo: | http://tede2.uefs.br:8080/handle/tede/1065 |
Resumo: | The Witches’ Broom Disease, caused by the hemibiotrophic basidiomycete fungus Moniliophthora perniciosa, drastically reduced the production of cocoa in Brazil. M. perniciosa colonize meristematic tissues decreasing the productivity and the lifetime of the host plant. Efforts have been expended to elucidate the molecular targets, such as beta(1,3)-D-glucan synthase, a glycosyltransferase that is essential for the cell wall construction, this enzyme catalyzes the formation of 1,3-β-D-glycans structural compounds. Bioinformatics methods are able to determine the structure of proteins without performing experimental steps, considering the barriers related to experimental methods for structure determination of molecular targets. Therefore, the objective of this study was determine the three-dimensional model of the enzyme beta(1,3)-D-glucan synthase of M. perniciosa by homology modeling and study the micelial growth inhibiting of the fungus by organics compounds and vegetal extracts with potential use in cocoa plantation. To build the models, was implemented both procedures: a comparative modeling by satisfaction of spatial restraints in MODELLER and a modeling by assembly of rigid bodies in the SWISS-MODEL software. Was obtained reasonable for beta(1,3)-D-glucan synthase enzyme (BegS1), agree well with general structure from GT-2 enzyme family. In studies of fungal inhibiting growth, the MIC was considered one which there was 100% inhibition of mycelial growth. Extracts derived from Lippia alnifolia and the substance HQN showed promising results with MIC lowest values for M. perniciosa fungus. |
id |
UEFS_97882504073b462336770dedec56830f |
---|---|
oai_identifier_str |
oai:tede2.uefs.br:8080:tede/1065 |
network_acronym_str |
UEFS |
network_name_str |
Biblioteca Digital de Teses e Dissertações da UEFS |
repository_id_str |
|
spelling |
Assis, Sandra Aparecida de9806301455398063014553http://lattes.cnpq.br/4044806953310324Pinheiro, Antonio Anderson Freitas2020-05-01T14:42:42Z2011-03-24PINHEIRO, Antonio Anderson Freitas. Estudos de inibição e modelagem por homologia da enzima beta(1,3)-d-glicano sintase de moniliophthora perniciosa. 2011. 109 f. Dissertação (Mestrado Acadêmico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2011.http://tede2.uefs.br:8080/handle/tede/1065The Witches’ Broom Disease, caused by the hemibiotrophic basidiomycete fungus Moniliophthora perniciosa, drastically reduced the production of cocoa in Brazil. M. perniciosa colonize meristematic tissues decreasing the productivity and the lifetime of the host plant. Efforts have been expended to elucidate the molecular targets, such as beta(1,3)-D-glucan synthase, a glycosyltransferase that is essential for the cell wall construction, this enzyme catalyzes the formation of 1,3-β-D-glycans structural compounds. Bioinformatics methods are able to determine the structure of proteins without performing experimental steps, considering the barriers related to experimental methods for structure determination of molecular targets. Therefore, the objective of this study was determine the three-dimensional model of the enzyme beta(1,3)-D-glucan synthase of M. perniciosa by homology modeling and study the micelial growth inhibiting of the fungus by organics compounds and vegetal extracts with potential use in cocoa plantation. To build the models, was implemented both procedures: a comparative modeling by satisfaction of spatial restraints in MODELLER and a modeling by assembly of rigid bodies in the SWISS-MODEL software. Was obtained reasonable for beta(1,3)-D-glucan synthase enzyme (BegS1), agree well with general structure from GT-2 enzyme family. In studies of fungal inhibiting growth, the MIC was considered one which there was 100% inhibition of mycelial growth. Extracts derived from Lippia alnifolia and the substance HQN showed promising results with MIC lowest values for M. perniciosa fungus.A doença da Vassoura-de-Bruxa, causada pelo fungo basidiomiceto hemibiotrófico Moniliophthora perniciosa, reduziu drasticamente a produção de cacau no Brasil. O M. perniciosa coloniza os tecidos meristemáticos diminuindo a produtividade, bem como o tempo de vida da planta hospedeira. Esforços têm sido despendidos para elucidação de alvos moleculares, como a beta(1,3)-D-glicano sintase, que é uma glicosiltransferase fundamental para a construção da parede celular e catalisa a formação dos compostos estruturais beta(1,3)-glicanos. Métodos de bioinformática são capazes de determinar a estrutura das proteínas sem a realização de etapas experimentais, considerando as dificuldades relacionadas aos métodos experimentais para determinação da estrutura de alvos moleculares. Portanto, o objetivo deste estudo foi determinar o modelo tridimensional da enzima sintase do beta(1,3)-D-glicano de M. perniciosa pela modelagem por homologia e estudar a inibição do crescimento micelial do fungo por compostos organicos e extratos vegetais com uso potencial em plantações de cacau. Para construir os modelos, foram executadas duas metodologias: através da modelagem comparativa por satisfação de restrições espaciais no MODELLER e por meio da metodologia de montagem de corpos rígidos no software SWISS-MODEL. Foi obtido modelo com estrutura razoável para a enzima beta(1,3)-D-glucano sintase (BegS1), este modelo apresentou concordância com a estrutural geral da família de enzimas GT-2. Nos estudos de inibição do crescimento fúngico, a CIM foi considerada aquela onde existiu inibição de 100% do crescimento micelial. Extratos obtidos a partir de Lippia alnifolia e a substância HQN revelaram resultados promissores com menores valores de CIM para o fungo M. perniciosa.Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2020-05-01T14:42:42Z No. of bitstreams: 1 Dissertação AAFPinheiro.pdf: 6582051 bytes, checksum: f96b8fc347ec5d82593310dceab0d257 (MD5)Made available in DSpace on 2020-05-01T14:42:42Z (GMT). No. of bitstreams: 1 Dissertação AAFPinheiro.pdf: 6582051 bytes, checksum: f96b8fc347ec5d82593310dceab0d257 (MD5) Previous issue date: 2011-03-24application/pdfporUniversidade Estadual de Feira de SantanaMestrado Acadêmico em BiotecnologiaUEFSBrasilDEPARTAMENTO DE CIÊNCIAS BIOLÓGICASDoença da Vassoura-de-BruxaMoniliophthora perniciosaModelagem por homologiaBeta(1,3)-D-glicano sintaseGlicosiltransferasesInibição do crescimento fúngicoWitches’ Broom DiseaseMoniliophora perniciosaHomology modelingBeta(1,3)-D-glucan synthaseGlycosyltransferaseFungal growth inhibitionCIENCIAS BIOLOGICAS::BIOLOGIA GERALCIENCIAS BIOLOGICASEstudos de inibição e modelagem por homologia da enzima beta(1,3)-d-glicano sintase de moniliophthora perniciosainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-54735432730516527826006006006005026123383450589282-1634559385931244697-3439178843068202161info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEFSinstname:Universidade Estadual de Feira de Santana (UEFS)instacron:UEFSORIGINALDissertação AAFPinheiro.pdfDissertação AAFPinheiro.pdfapplication/pdf6582051http://tede2.uefs.br:8080/bitstream/tede/1065/2/Disserta%C3%A7%C3%A3o+AAFPinheiro.pdff96b8fc347ec5d82593310dceab0d257MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82089http://tede2.uefs.br:8080/bitstream/tede/1065/1/license.txt7b5ba3d2445355f386edab96125d42b7MD51tede/10652020-05-01 11:42:42.14oai:tede2.uefs.br:8080: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.uefs.br:8080/PUBhttp://tede2.uefs.br:8080/oai/requestbcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.bropendoar:2020-05-01T14:42:42Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS)false |
dc.title.por.fl_str_mv |
Estudos de inibição e modelagem por homologia da enzima beta(1,3)-d-glicano sintase de moniliophthora perniciosa |
title |
Estudos de inibição e modelagem por homologia da enzima beta(1,3)-d-glicano sintase de moniliophthora perniciosa |
spellingShingle |
Estudos de inibição e modelagem por homologia da enzima beta(1,3)-d-glicano sintase de moniliophthora perniciosa Pinheiro, Antonio Anderson Freitas Doença da Vassoura-de-Bruxa Moniliophthora perniciosa Modelagem por homologia Beta(1,3)-D-glicano sintase Glicosiltransferases Inibição do crescimento fúngico Witches’ Broom Disease Moniliophora perniciosa Homology modeling Beta(1,3)-D-glucan synthase Glycosyltransferase Fungal growth inhibition CIENCIAS BIOLOGICAS::BIOLOGIA GERAL CIENCIAS BIOLOGICAS |
title_short |
Estudos de inibição e modelagem por homologia da enzima beta(1,3)-d-glicano sintase de moniliophthora perniciosa |
title_full |
Estudos de inibição e modelagem por homologia da enzima beta(1,3)-d-glicano sintase de moniliophthora perniciosa |
title_fullStr |
Estudos de inibição e modelagem por homologia da enzima beta(1,3)-d-glicano sintase de moniliophthora perniciosa |
title_full_unstemmed |
Estudos de inibição e modelagem por homologia da enzima beta(1,3)-d-glicano sintase de moniliophthora perniciosa |
title_sort |
Estudos de inibição e modelagem por homologia da enzima beta(1,3)-d-glicano sintase de moniliophthora perniciosa |
author |
Pinheiro, Antonio Anderson Freitas |
author_facet |
Pinheiro, Antonio Anderson Freitas |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Assis, Sandra Aparecida de |
dc.contributor.advisor1ID.fl_str_mv |
98063014553 |
dc.contributor.authorID.fl_str_mv |
98063014553 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/4044806953310324 |
dc.contributor.author.fl_str_mv |
Pinheiro, Antonio Anderson Freitas |
contributor_str_mv |
Assis, Sandra Aparecida de |
dc.subject.por.fl_str_mv |
Doença da Vassoura-de-Bruxa Moniliophthora perniciosa Modelagem por homologia Beta(1,3)-D-glicano sintase Glicosiltransferases Inibição do crescimento fúngico |
topic |
Doença da Vassoura-de-Bruxa Moniliophthora perniciosa Modelagem por homologia Beta(1,3)-D-glicano sintase Glicosiltransferases Inibição do crescimento fúngico Witches’ Broom Disease Moniliophora perniciosa Homology modeling Beta(1,3)-D-glucan synthase Glycosyltransferase Fungal growth inhibition CIENCIAS BIOLOGICAS::BIOLOGIA GERAL CIENCIAS BIOLOGICAS |
dc.subject.eng.fl_str_mv |
Witches’ Broom Disease Moniliophora perniciosa Homology modeling Beta(1,3)-D-glucan synthase Glycosyltransferase Fungal growth inhibition |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL CIENCIAS BIOLOGICAS |
description |
The Witches’ Broom Disease, caused by the hemibiotrophic basidiomycete fungus Moniliophthora perniciosa, drastically reduced the production of cocoa in Brazil. M. perniciosa colonize meristematic tissues decreasing the productivity and the lifetime of the host plant. Efforts have been expended to elucidate the molecular targets, such as beta(1,3)-D-glucan synthase, a glycosyltransferase that is essential for the cell wall construction, this enzyme catalyzes the formation of 1,3-β-D-glycans structural compounds. Bioinformatics methods are able to determine the structure of proteins without performing experimental steps, considering the barriers related to experimental methods for structure determination of molecular targets. Therefore, the objective of this study was determine the three-dimensional model of the enzyme beta(1,3)-D-glucan synthase of M. perniciosa by homology modeling and study the micelial growth inhibiting of the fungus by organics compounds and vegetal extracts with potential use in cocoa plantation. To build the models, was implemented both procedures: a comparative modeling by satisfaction of spatial restraints in MODELLER and a modeling by assembly of rigid bodies in the SWISS-MODEL software. Was obtained reasonable for beta(1,3)-D-glucan synthase enzyme (BegS1), agree well with general structure from GT-2 enzyme family. In studies of fungal inhibiting growth, the MIC was considered one which there was 100% inhibition of mycelial growth. Extracts derived from Lippia alnifolia and the substance HQN showed promising results with MIC lowest values for M. perniciosa fungus. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-03-24 |
dc.date.accessioned.fl_str_mv |
2020-05-01T14:42:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PINHEIRO, Antonio Anderson Freitas. Estudos de inibição e modelagem por homologia da enzima beta(1,3)-d-glicano sintase de moniliophthora perniciosa. 2011. 109 f. Dissertação (Mestrado Acadêmico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2011. |
dc.identifier.uri.fl_str_mv |
http://tede2.uefs.br:8080/handle/tede/1065 |
identifier_str_mv |
PINHEIRO, Antonio Anderson Freitas. Estudos de inibição e modelagem por homologia da enzima beta(1,3)-d-glicano sintase de moniliophthora perniciosa. 2011. 109 f. Dissertação (Mestrado Acadêmico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2011. |
url |
http://tede2.uefs.br:8080/handle/tede/1065 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
-5473543273051652782 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 |
dc.relation.department.fl_str_mv |
5026123383450589282 |
dc.relation.cnpq.fl_str_mv |
-1634559385931244697 -3439178843068202161 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Feira de Santana |
dc.publisher.program.fl_str_mv |
Mestrado Acadêmico em Biotecnologia |
dc.publisher.initials.fl_str_mv |
UEFS |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
DEPARTAMENTO DE CIÊNCIAS BIOLÓGICAS |
publisher.none.fl_str_mv |
Universidade Estadual de Feira de Santana |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UEFS instname:Universidade Estadual de Feira de Santana (UEFS) instacron:UEFS |
instname_str |
Universidade Estadual de Feira de Santana (UEFS) |
instacron_str |
UEFS |
institution |
UEFS |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UEFS |
collection |
Biblioteca Digital de Teses e Dissertações da UEFS |
bitstream.url.fl_str_mv |
http://tede2.uefs.br:8080/bitstream/tede/1065/2/Disserta%C3%A7%C3%A3o+AAFPinheiro.pdf http://tede2.uefs.br:8080/bitstream/tede/1065/1/license.txt |
bitstream.checksum.fl_str_mv |
f96b8fc347ec5d82593310dceab0d257 7b5ba3d2445355f386edab96125d42b7 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS) |
repository.mail.fl_str_mv |
bcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.br |
_version_ |
1809288779871027200 |