Isolamento e identificação de fungos endofiticos de Mikania laevigata (asteroceae) e análise química dos extratos por clae-dad
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UEFS |
Texto Completo: | http://tede2.uefs.br:8080/handle/tede/1106 |
Resumo: | The interactions between fungi and plants, especially endophytic fungi, are considered important sources of secondary metabolites. The detection of these microorganisms inside healthy plants has opened new perspectives for the study of fungus-plant interactions. This study aimed to isolate and identify the molecular analysis of endophytic fungi of Mikania laevigata (Asteraceae) and perform chemical analysis by HPLC-DAD extracts of these fungi. The leaves of Mikania laevigata grown in tree farm were subjected to the isolation procedure that resulted in endophytic fungi isolated 40. Of these, 15 were selected and characterized by morphological features as Xylariaceae ascomycetes family. Molecular investigation by sequencing the rDNA ITS region resulted in the identification of three strains of Nodulisporium sp., 3 strains of Hypoxylon sp. Daldinia sp 1 strain. and a species identified as Xylaria luteostromata. Could not be identified at the molecular level the other samples subjected to sequencing. The analysis by HPLC-DAD showed a large chemical diversity among the analyzed extracts. It was noted the presence of a greater amount of metabolites in the extracts compared to the external micellar extracts. The methanol extracts showed the presence of a greater number of metabolites when compared to extracts in ethyl acetate. |
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Branco, Alexsandro9234396197293536747515http://lattes.cnpq.br/3497222662010996Ribeiro, Fernanda Pinheiro Carvalho2020-05-08T00:11:03Z2011-08-12RIBEIRO, Fernanda Pinheiro de Carvalho. Isolamento e identificação de fungos endofiticos de Mikania laevigata (asteroceae) e análise química dos extratos por clae-dad. 2011. 139 f. Dissertação (Mestrado Acadêmico em Recursos Genéticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2011.http://tede2.uefs.br:8080/handle/tede/1106The interactions between fungi and plants, especially endophytic fungi, are considered important sources of secondary metabolites. The detection of these microorganisms inside healthy plants has opened new perspectives for the study of fungus-plant interactions. This study aimed to isolate and identify the molecular analysis of endophytic fungi of Mikania laevigata (Asteraceae) and perform chemical analysis by HPLC-DAD extracts of these fungi. The leaves of Mikania laevigata grown in tree farm were subjected to the isolation procedure that resulted in endophytic fungi isolated 40. Of these, 15 were selected and characterized by morphological features as Xylariaceae ascomycetes family. Molecular investigation by sequencing the rDNA ITS region resulted in the identification of three strains of Nodulisporium sp., 3 strains of Hypoxylon sp. Daldinia sp 1 strain. and a species identified as Xylaria luteostromata. Could not be identified at the molecular level the other samples subjected to sequencing. The analysis by HPLC-DAD showed a large chemical diversity among the analyzed extracts. It was noted the presence of a greater amount of metabolites in the extracts compared to the external micellar extracts. The methanol extracts showed the presence of a greater number of metabolites when compared to extracts in ethyl acetate.As interações entre fungos e plantas, especialmente fungos endofíticos, são consideradas importantes fontes geradoras de metabólitos secundários. A detecção destes microrganismos no interior de plantas sadias abriu novas perspectivas para o estudo das interações fungos-planta. O presente trabalho teve como objetivo isolar e identificar por análise molecular os fungos endofíticos de Mikania laevigata (Asteraceae) e realizar a análise química por CLAE-DAD dos extratos obtidos destes fungos. As folhas de Mikania laevigata, cultivadas em horto florestal, foram submetidas a procedimento de isolamento dos fungos endofíticos que resultou em 40 isolados. Destes, 15 foram selecionados e caracterizados pelos aspectos morfológicos como ascomicetos da família Xylariaceae. A investigação molecular através do sequenciamento da região ITS rDNA resultou na identificação de 3 cepas de Nodulisporium sp., 3 cepas de Hypoxylon sp., 1 cepa de Daldinia sp. e 1 espécie identificada como Xylaria luteostromata. Não foi possível identificar em nível molecular as demais amostras submetidas ao seqüenciamento. A análise por CLAE-DAD revelou grande diversidade química entre os extratos analisados. Notou-se a presença de uma maior quantidade de metabólitos nos extratos externos em comparação com os extratos micelares. Os extratos metanólicos mostraram a presença de um maior número de metabólitos quando comparados aos extratos em acetato de etila.Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2020-05-08T00:11:03Z No. of bitstreams: 1 Monografia.pdf: 3369567 bytes, checksum: 076a115698fc0d6aaa3cb19b76bae2c9 (MD5)Made available in DSpace on 2020-05-08T00:11:03Z (GMT). No. of bitstreams: 1 Monografia.pdf: 3369567 bytes, checksum: 076a115698fc0d6aaa3cb19b76bae2c9 (MD5) Previous issue date: 2011-08-12application/pdfporUniversidade Estadual de Feira de SantanaMestrado Acadêmico em Recursos Genéticos VegetaisUEFSBrasilDEPARTAMENTO DE CIÊNCIAS BIOLÓGICASPlanta medicinalMikania laevigataMetabólitos secundáriosMedicinal plantsSecondary metabolitesCIENCIAS AGRARIASIsolamento e identificação de fungos endofiticos de Mikania laevigata (asteroceae) e análise química dos extratos por clae-dadinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis814664648162224187460060060050261233834505892827828424726906663919info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEFSinstname:Universidade Estadual de Feira de Santana (UEFS)instacron:UEFSORIGINALMonografia.pdfMonografia.pdfapplication/pdf3369567http://tede2.uefs.br:8080/bitstream/tede/1106/2/Monografia.pdf076a115698fc0d6aaa3cb19b76bae2c9MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82089http://tede2.uefs.br:8080/bitstream/tede/1106/1/license.txt7b5ba3d2445355f386edab96125d42b7MD51tede/11062020-05-07 21:11:03.347oai:tede2.uefs.br:8080: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.uefs.br:8080/PUBhttp://tede2.uefs.br:8080/oai/requestbcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.bropendoar:2020-05-08T00:11:03Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS)false |
dc.title.por.fl_str_mv |
Isolamento e identificação de fungos endofiticos de Mikania laevigata (asteroceae) e análise química dos extratos por clae-dad |
title |
Isolamento e identificação de fungos endofiticos de Mikania laevigata (asteroceae) e análise química dos extratos por clae-dad |
spellingShingle |
Isolamento e identificação de fungos endofiticos de Mikania laevigata (asteroceae) e análise química dos extratos por clae-dad Ribeiro, Fernanda Pinheiro Carvalho Planta medicinal Mikania laevigata Metabólitos secundários Medicinal plants Secondary metabolites CIENCIAS AGRARIAS |
title_short |
Isolamento e identificação de fungos endofiticos de Mikania laevigata (asteroceae) e análise química dos extratos por clae-dad |
title_full |
Isolamento e identificação de fungos endofiticos de Mikania laevigata (asteroceae) e análise química dos extratos por clae-dad |
title_fullStr |
Isolamento e identificação de fungos endofiticos de Mikania laevigata (asteroceae) e análise química dos extratos por clae-dad |
title_full_unstemmed |
Isolamento e identificação de fungos endofiticos de Mikania laevigata (asteroceae) e análise química dos extratos por clae-dad |
title_sort |
Isolamento e identificação de fungos endofiticos de Mikania laevigata (asteroceae) e análise química dos extratos por clae-dad |
author |
Ribeiro, Fernanda Pinheiro Carvalho |
author_facet |
Ribeiro, Fernanda Pinheiro Carvalho |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Branco, Alexsandro |
dc.contributor.advisor1ID.fl_str_mv |
92343961972 |
dc.contributor.authorID.fl_str_mv |
93536747515 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3497222662010996 |
dc.contributor.author.fl_str_mv |
Ribeiro, Fernanda Pinheiro Carvalho |
contributor_str_mv |
Branco, Alexsandro |
dc.subject.por.fl_str_mv |
Planta medicinal Mikania laevigata Metabólitos secundários |
topic |
Planta medicinal Mikania laevigata Metabólitos secundários Medicinal plants Secondary metabolites CIENCIAS AGRARIAS |
dc.subject.eng.fl_str_mv |
Medicinal plants Secondary metabolites |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS |
description |
The interactions between fungi and plants, especially endophytic fungi, are considered important sources of secondary metabolites. The detection of these microorganisms inside healthy plants has opened new perspectives for the study of fungus-plant interactions. This study aimed to isolate and identify the molecular analysis of endophytic fungi of Mikania laevigata (Asteraceae) and perform chemical analysis by HPLC-DAD extracts of these fungi. The leaves of Mikania laevigata grown in tree farm were subjected to the isolation procedure that resulted in endophytic fungi isolated 40. Of these, 15 were selected and characterized by morphological features as Xylariaceae ascomycetes family. Molecular investigation by sequencing the rDNA ITS region resulted in the identification of three strains of Nodulisporium sp., 3 strains of Hypoxylon sp. Daldinia sp 1 strain. and a species identified as Xylaria luteostromata. Could not be identified at the molecular level the other samples subjected to sequencing. The analysis by HPLC-DAD showed a large chemical diversity among the analyzed extracts. It was noted the presence of a greater amount of metabolites in the extracts compared to the external micellar extracts. The methanol extracts showed the presence of a greater number of metabolites when compared to extracts in ethyl acetate. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-08-12 |
dc.date.accessioned.fl_str_mv |
2020-05-08T00:11:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
RIBEIRO, Fernanda Pinheiro de Carvalho. Isolamento e identificação de fungos endofiticos de Mikania laevigata (asteroceae) e análise química dos extratos por clae-dad. 2011. 139 f. Dissertação (Mestrado Acadêmico em Recursos Genéticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2011. |
dc.identifier.uri.fl_str_mv |
http://tede2.uefs.br:8080/handle/tede/1106 |
identifier_str_mv |
RIBEIRO, Fernanda Pinheiro de Carvalho. Isolamento e identificação de fungos endofiticos de Mikania laevigata (asteroceae) e análise química dos extratos por clae-dad. 2011. 139 f. Dissertação (Mestrado Acadêmico em Recursos Genéticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2011. |
url |
http://tede2.uefs.br:8080/handle/tede/1106 |
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por |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Estadual de Feira de Santana |
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Mestrado Acadêmico em Recursos Genéticos Vegetais |
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UEFS |
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DEPARTAMENTO DE CIÊNCIAS BIOLÓGICAS |
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Universidade Estadual de Feira de Santana |
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Biblioteca Digital de Teses e Dissertações da UEFS |
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