Obtenção, caracterização e atividade antitumoral in vitro de bromelina de diferentes partes de abacaxizeiros
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UEFS |
Texto Completo: | http://localhost:8080/tede/handle/tede/125 |
Resumo: | Bromelain is an enzyme complex with great therapeutic importance attributed both to it’s proteolytic activity, as the non-proteolytic components in the extract. Thus, the enzyme was extracted from different parts of Ananas comosus var comosus, from two pineapple with different codes: BGA 771 and BGA 772. The proteolytic and specific activities of bromelain from each part (stem, crown, peel, pulp and leaves) were investigated as well as their kinetic characteristics by measuring the optimum temperature and the optimum pH, and finally was studied it’s effect in vitro on murine melanoma B16F10 cells. In BGA 771, the highest proteolytic activity was found in the stem (81.10 mg/mL/min), while the highest specific activity was found in the pulp and peel (1983.44 and 1822.33 UA/mg of bromelain, respectively). In general, the optimum temperature varied between of 50 to 80 °C and pH between 6 and 8. Bromelain all parts were effective more than 50% in inhibiting the melanoma B16F10 cells proliferation after 48 hours. The most significant inhibition was find on crown bromelain with 72.86%, and a time-dependent effect was observed on crown bromelain. At BGA 772 the higher activity was find in the pulp (35.58 mg/mL/min and 1446.60 UA/mg bromelain). The optimum temperature in the extracts ranged between 40 and 90 °C, while the pH 6 was the best for all parties, while the leaf bromelain had two great points: 6 and 8. Only the bromelain peel was able to inhibit the melanoma B16F10 proliferation of, being 66.37% after 48 hours of contact, and a dose-dependent activity was observed. |
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Assis, Sandra Aparecida de03589502525http://lattes.cnpq.br/5825099358240776Miranda, Íngara Keisle São Paulo Barretto2015-07-28T23:56:29Z2014-03-25MIRANDA, Íngara Keisle São Paulo Barretto. Obtenção, caracterização e atividade antitumoral in vitro de bromelina de diferentes partes de abacaxizeiros. 2014. 79 f. Dissertação (Mestrado Acadêmico em Recursos Genéticos Vegetais) - Universidade Estadual de Feira de Santana, Feira de Santana, 2014.http://localhost:8080/tede/handle/tede/125Bromelain is an enzyme complex with great therapeutic importance attributed both to it’s proteolytic activity, as the non-proteolytic components in the extract. Thus, the enzyme was extracted from different parts of Ananas comosus var comosus, from two pineapple with different codes: BGA 771 and BGA 772. The proteolytic and specific activities of bromelain from each part (stem, crown, peel, pulp and leaves) were investigated as well as their kinetic characteristics by measuring the optimum temperature and the optimum pH, and finally was studied it’s effect in vitro on murine melanoma B16F10 cells. In BGA 771, the highest proteolytic activity was found in the stem (81.10 mg/mL/min), while the highest specific activity was found in the pulp and peel (1983.44 and 1822.33 UA/mg of bromelain, respectively). In general, the optimum temperature varied between of 50 to 80 °C and pH between 6 and 8. Bromelain all parts were effective more than 50% in inhibiting the melanoma B16F10 cells proliferation after 48 hours. The most significant inhibition was find on crown bromelain with 72.86%, and a time-dependent effect was observed on crown bromelain. At BGA 772 the higher activity was find in the pulp (35.58 mg/mL/min and 1446.60 UA/mg bromelain). The optimum temperature in the extracts ranged between 40 and 90 °C, while the pH 6 was the best for all parties, while the leaf bromelain had two great points: 6 and 8. Only the bromelain peel was able to inhibit the melanoma B16F10 proliferation of, being 66.37% after 48 hours of contact, and a dose-dependent activity was observed.A bromelina é um complexo enzimático de grande importância terapêutica, atribuída tanto à sua atividade proteolítica, como aos componentes não proteolíticos contidos no extrato. Dessa forma, a enzima foi extraída de diferentes partes de Ananas comosus var comosus, a partir de dois abacaxizeiros com identificações distintas: BGA 771 e BGA 772. Foram investigadas as atividades proteolítica e específica da bromelina de cada parte (caule, coroa, casca, polpa e folha), bem como suas características cinéticas através da medida da temperatura e do pH ótimos, investigando-se, por fim, o seu efeito in vitro sobre células tumorais de melanoma murino B16F10. No BGA 771, a maior atividade proteolítica foi encontrada no caule (81,10 μg/mL/min), enquanto a maior atividade específica foi encontrada na polpa e na casca (1983,44 e 1822,33 UA/mg de bromelina, respectivamente). De um modo geral, a temperatura ótima variou entre as partes de 50 a 80 °C e o pH ótimo entre 6 e 8. A bromelina de todas as partes foi eficaz em inibir a proliferação das células de melanoma B16F10 após 48 horas em mais de 50%, sendo a da coroa a mais significativa, com 72,86% de inibição, tendo sido observada uma atividade tempo-dependente. No BGA 772, as maiores atividade foram encontradas na polpa (35,58 μg/mL/min e 1446,60 UA/mg de bromelina). A temperatura ótima nos extratos variou entre 40 e 90°C, enquanto o pH 6 foi o melhor para todas as partes, exceto para a folha que teve dois pontos ótimos: 6 e 8. Somente a bromelina da casca foi capaz de inibir a proliferação do melanoma B16F10, sendo de 66,37% após 48 horas de contato, tendo sido observada uma atividade dose-dependente.Submitted by Natalie Mendes (nataliermendes@gmail.com) on 2015-07-28T23:56:29Z No. of bitstreams: 1 Dissertação_Íngara Keisle São Paulo Barretto Miranda.pdf: 984548 bytes, checksum: 4e0290fe54977157fb35593ccdb7ef99 (MD5)Made available in DSpace on 2015-07-28T23:56:29Z (GMT). No. of bitstreams: 1 Dissertação_Íngara Keisle São Paulo Barretto Miranda.pdf: 984548 bytes, checksum: 4e0290fe54977157fb35593ccdb7ef99 (MD5) Previous issue date: 2014-03-25Fundação de Amparo à Pesquisa do Estado da Bahia - FAPEBapplication/pdfporUniversidade Estadual de Feira de SantanaMestrado Acadêmico em Recursos Genéticos VegetaisUEFSBrasilDEPARTAMENTO DE CIÊNCIAS BIOLÓGICASProteaseAbacaxiAtividade antineoplásicaEfeito antiproliferativoProteasePineappleAntineoplastic activityCIENCIAS BIOLOGICASObtenção, caracterização e atividade antitumoral in vitro de bromelina de diferentes partes de abacaxizeirosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis2441563959449389152600600600600-6971480722008537872-34391788430682021614767858349021390776info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEFSinstname:Universidade Estadual de Feira de Santana (UEFS)instacron:UEFSORIGINALDissertação_Íngara Keisle São Paulo Barretto Miranda.pdfDissertação_Íngara Keisle São Paulo Barretto Miranda.pdfapplication/pdf984548http://tede2.uefs.br:8080/bitstream/tede/125/2/Disserta%C3%A7%C3%A3o_%C3%8Dngara+Keisle+S%C3%A3o+Paulo+Barretto+Miranda.pdf4e0290fe54977157fb35593ccdb7ef99MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://tede2.uefs.br:8080/bitstream/tede/125/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/1252016-01-13 21:06:19.417oai:tede2.uefs.br:8080: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.uefs.br:8080/PUBhttp://tede2.uefs.br:8080/oai/requestbcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.bropendoar:2016-01-14T00:06:19Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS)false |
dc.title.por.fl_str_mv |
Obtenção, caracterização e atividade antitumoral in vitro de bromelina de diferentes partes de abacaxizeiros |
title |
Obtenção, caracterização e atividade antitumoral in vitro de bromelina de diferentes partes de abacaxizeiros |
spellingShingle |
Obtenção, caracterização e atividade antitumoral in vitro de bromelina de diferentes partes de abacaxizeiros Miranda, Íngara Keisle São Paulo Barretto Protease Abacaxi Atividade antineoplásica Efeito antiproliferativo Protease Pineapple Antineoplastic activity CIENCIAS BIOLOGICAS |
title_short |
Obtenção, caracterização e atividade antitumoral in vitro de bromelina de diferentes partes de abacaxizeiros |
title_full |
Obtenção, caracterização e atividade antitumoral in vitro de bromelina de diferentes partes de abacaxizeiros |
title_fullStr |
Obtenção, caracterização e atividade antitumoral in vitro de bromelina de diferentes partes de abacaxizeiros |
title_full_unstemmed |
Obtenção, caracterização e atividade antitumoral in vitro de bromelina de diferentes partes de abacaxizeiros |
title_sort |
Obtenção, caracterização e atividade antitumoral in vitro de bromelina de diferentes partes de abacaxizeiros |
author |
Miranda, Íngara Keisle São Paulo Barretto |
author_facet |
Miranda, Íngara Keisle São Paulo Barretto |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Assis, Sandra Aparecida de |
dc.contributor.authorID.fl_str_mv |
03589502525 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5825099358240776 |
dc.contributor.author.fl_str_mv |
Miranda, Íngara Keisle São Paulo Barretto |
contributor_str_mv |
Assis, Sandra Aparecida de |
dc.subject.por.fl_str_mv |
Protease Abacaxi Atividade antineoplásica Efeito antiproliferativo |
topic |
Protease Abacaxi Atividade antineoplásica Efeito antiproliferativo Protease Pineapple Antineoplastic activity CIENCIAS BIOLOGICAS |
dc.subject.eng.fl_str_mv |
Protease Pineapple Antineoplastic activity |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS |
description |
Bromelain is an enzyme complex with great therapeutic importance attributed both to it’s proteolytic activity, as the non-proteolytic components in the extract. Thus, the enzyme was extracted from different parts of Ananas comosus var comosus, from two pineapple with different codes: BGA 771 and BGA 772. The proteolytic and specific activities of bromelain from each part (stem, crown, peel, pulp and leaves) were investigated as well as their kinetic characteristics by measuring the optimum temperature and the optimum pH, and finally was studied it’s effect in vitro on murine melanoma B16F10 cells. In BGA 771, the highest proteolytic activity was found in the stem (81.10 mg/mL/min), while the highest specific activity was found in the pulp and peel (1983.44 and 1822.33 UA/mg of bromelain, respectively). In general, the optimum temperature varied between of 50 to 80 °C and pH between 6 and 8. Bromelain all parts were effective more than 50% in inhibiting the melanoma B16F10 cells proliferation after 48 hours. The most significant inhibition was find on crown bromelain with 72.86%, and a time-dependent effect was observed on crown bromelain. At BGA 772 the higher activity was find in the pulp (35.58 mg/mL/min and 1446.60 UA/mg bromelain). The optimum temperature in the extracts ranged between 40 and 90 °C, while the pH 6 was the best for all parties, while the leaf bromelain had two great points: 6 and 8. Only the bromelain peel was able to inhibit the melanoma B16F10 proliferation of, being 66.37% after 48 hours of contact, and a dose-dependent activity was observed. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-03-25 |
dc.date.accessioned.fl_str_mv |
2015-07-28T23:56:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MIRANDA, Íngara Keisle São Paulo Barretto. Obtenção, caracterização e atividade antitumoral in vitro de bromelina de diferentes partes de abacaxizeiros. 2014. 79 f. Dissertação (Mestrado Acadêmico em Recursos Genéticos Vegetais) - Universidade Estadual de Feira de Santana, Feira de Santana, 2014. |
dc.identifier.uri.fl_str_mv |
http://localhost:8080/tede/handle/tede/125 |
identifier_str_mv |
MIRANDA, Íngara Keisle São Paulo Barretto. Obtenção, caracterização e atividade antitumoral in vitro de bromelina de diferentes partes de abacaxizeiros. 2014. 79 f. Dissertação (Mestrado Acadêmico em Recursos Genéticos Vegetais) - Universidade Estadual de Feira de Santana, Feira de Santana, 2014. |
url |
http://localhost:8080/tede/handle/tede/125 |
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por |
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por |
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2441563959449389152 |
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600 600 600 600 |
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4767858349021390776 |
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openAccess |
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Universidade Estadual de Feira de Santana |
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Mestrado Acadêmico em Recursos Genéticos Vegetais |
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UEFS |
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Brasil |
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DEPARTAMENTO DE CIÊNCIAS BIOLÓGICAS |
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Universidade Estadual de Feira de Santana |
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