Avaliação da atividade fotoprotetora in vitro de extratos etanólicos de três espécies de Marcetia e suas formulações
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UEFS |
Texto Completo: | http://localhost:8080/tede/handle/tede/339 |
Resumo: | Solar radiation is the main factor in the appearance of skin diseases. Preventive measures against skin photoaging are necessary and one of the ways to avoid it is to use sunscreen. The genus Marcetia (Melastomataceae) is endemic of rocky fields in northeastern Brazil and are adapted to high sunlight conditions due to bioproduction of flavonoids. The initial goal of this work was to evaluate the antioxidant activities, photoprotection (UVB/UVA) and toxicity of ethanolic extracts of M. taxifolia, M. macrophylla and M. latifolia. Thus, it was investigated the potential photoprotection of the extracts incorporated at 5, 10, 20 and 30% by polawax® lotion. All tests were performed in vitro. The formulations were evaluated in the physicochemical standpoint. In addition it was held HPLC-DAD for identification and quantification of flavonoids. The spectrophotometric method was used to determine the total level of flavonoids (TFC), DPPH• (antioxidant activity) and photoprotective activity (UVB/UVA). The evaluation of toxicity was made by means of the HET-CAM test, and hemolysis rate. The most promising extract was fractionated. Photoprotective formulations have been developed (5, 10, 20 and 30%), which also had their sunscreen profile UVB / UVA evaluated. The M. taxifolia showed higher quercetin content, with 55.78 g/Kg and 168 g/mL, following the studies of HPLC-DAD and TFT. All Marcetia ssp extracts had an antioxidant activity around 95%. Likewise, the three species had good results to SPF and UVA photoprotection. The species that has better value SPF was M. macrophylla (20.25) and UVA protection was M. taxifolia (86.04%), but these species have a higher toxic potential related to M. latifolia, which also had a good value SPF (13.12) and UVA (78.09%). The toxicity results show that this species is more secure and is considered an irritant and of weak hemolysis rate (23.51%), while the other species had a hemolysis rate 50%. Given these results EEML was fractionated into five fractions (MLFR02, MLFR03, MLFR04, MLFR05, MLFR06). After testing, MLFR02 showed good UVB sunscreen activity (17,52) and UVA (87.49%), but the safest was MLFR02 beyond presenting results in a high SPF value (37.82) when incorporated into the emulsion which makes it suitable for individuals who are very sensitive to sunburn. |
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Branco, Alexsandro94820635549http://lattes.cnpq.br/4166810899152932Costa, Sônia Carine Cova2016-06-14T22:48:14Z2015-12-17COSTA, Sônia Carine Cova. Avaliação da atividade fotoprotetora in vitro de extratos etanólicos de três espécies de Marcetia e suas formulações. 2015. 215 f. Tese (Doutorado Acadêmico em Recursos Genéticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2015.http://localhost:8080/tede/handle/tede/339Solar radiation is the main factor in the appearance of skin diseases. Preventive measures against skin photoaging are necessary and one of the ways to avoid it is to use sunscreen. The genus Marcetia (Melastomataceae) is endemic of rocky fields in northeastern Brazil and are adapted to high sunlight conditions due to bioproduction of flavonoids. The initial goal of this work was to evaluate the antioxidant activities, photoprotection (UVB/UVA) and toxicity of ethanolic extracts of M. taxifolia, M. macrophylla and M. latifolia. Thus, it was investigated the potential photoprotection of the extracts incorporated at 5, 10, 20 and 30% by polawax® lotion. All tests were performed in vitro. The formulations were evaluated in the physicochemical standpoint. In addition it was held HPLC-DAD for identification and quantification of flavonoids. The spectrophotometric method was used to determine the total level of flavonoids (TFC), DPPH• (antioxidant activity) and photoprotective activity (UVB/UVA). The evaluation of toxicity was made by means of the HET-CAM test, and hemolysis rate. The most promising extract was fractionated. Photoprotective formulations have been developed (5, 10, 20 and 30%), which also had their sunscreen profile UVB / UVA evaluated. The M. taxifolia showed higher quercetin content, with 55.78 g/Kg and 168 g/mL, following the studies of HPLC-DAD and TFT. All Marcetia ssp extracts had an antioxidant activity around 95%. Likewise, the three species had good results to SPF and UVA photoprotection. The species that has better value SPF was M. macrophylla (20.25) and UVA protection was M. taxifolia (86.04%), but these species have a higher toxic potential related to M. latifolia, which also had a good value SPF (13.12) and UVA (78.09%). The toxicity results show that this species is more secure and is considered an irritant and of weak hemolysis rate (23.51%), while the other species had a hemolysis rate 50%. Given these results EEML was fractionated into five fractions (MLFR02, MLFR03, MLFR04, MLFR05, MLFR06). After testing, MLFR02 showed good UVB sunscreen activity (17,52) and UVA (87.49%), but the safest was MLFR02 beyond presenting results in a high SPF value (37.82) when incorporated into the emulsion which makes it suitable for individuals who are very sensitive to sunburn.A radiação solar é o principal fator de surgimento de patologias tópicas. Medidas preventivas contra o fotoenvelhecimento cutâneo são necessárias e umas das formas é o uso de fotoprotetores. O gênero Marcetia (Melastomataceae) é endêmico dos campos rupestres do nordeste brasileiro e são adaptadas as condições de alta incidência solar devido à bioprodução de flavonoides. Este trabalho teve como objetivo inicial avaliar as atividades antioxidante, fotoprotetora (UVB/UVA) e toxicidade dos extratos etanólicos de M. taxifolia, M. macrophylla e M. latifolia. Em seguida, foi investigado o potencial fotoprotetor dos extratos incorporados a 5, 10, 20 e 30% em loção polawax®. Todos os testes foram realizados in vitro. As formulações foram avaliadas do ponto de vista físico-químico. Em adição realizou-se CLAE-DAD para identificação e quantificação dos flavonoides. O método espectrofotométrico foi usado para determinação do teor total de flavonoides (TFC), método DPPH (atividade antioxidante) e atividade fotoprotetora (UVB/UVA). A avaliação da toxicidade foi feita por meio do teste HET-CAM e hemólise. O extrato mais promissor foi fracionado. Foram desenvolvidas formulações fotoprotetoras (5, 10, 20 e 30%), as quais também tiveram seu perfil fotoprotetor UVB/UVA avaliados. A M. taxifolia apresentou maior teor de quercetina, com 55,78 g/Kg e 168 g/mL, conforme os estudos de CLAE-DAD e TFT. Todos os extratos de Marcetia ssp. tiveram uma atividade antioxidante em torno de 95%. Da mesma forma que as três espécies tiveram bons resultados de FPS e fotoproteção UVA. A espécie que apresentou melhor valor de FPS foi a M. macrophylla (20,25) e proteção UVA foi a M. taxifolia (86,04%), porém estas espécies tem um potencial tóxico superior a M. latifolia, que também teve um bom valor de FPS (13,12) e proteção UVA (78,09%). Os resultados de toxicidade mostram que esta espécie foi a mais segura, sendo considerada um irritante fraco e com uma taxa de hemólise de 23,51%, enquanto as outras espécies tiveram a taxa de hemólise 50%. Diante destes resultados o EEML foi fracionado em cinco frações (MLFR02, MLFR03, MLFR04, MLFR05, MLFR06). Após os ensaios, a MLFR02 apresentou boa atividade fotoprotetora UVB (FPS: 17, 52) e UVA (87,49%), porém a mais segura foi a MLFR02, além de apresentar um alto valor FPS (37,82) quando incorporada à emulsão o que a faz ser indicada para indivíduos muito sensíveis a queimadura solar.Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2016-06-14T22:48:14Z No. of bitstreams: 1 TESE SÔNIA CARINE COVA COSTA.pdf: 4450540 bytes, checksum: 273e33d63aa35b23e7686b53ad4e5b56 (MD5)Made available in DSpace on 2016-06-14T22:48:14Z (GMT). No. of bitstreams: 1 TESE SÔNIA CARINE COVA COSTA.pdf: 4450540 bytes, checksum: 273e33d63aa35b23e7686b53ad4e5b56 (MD5) Previous issue date: 2015-12-17application/pdfporUniversidade Estadual de Feira de SantanaDoutorado Acadêmico em Recursos Genéticos VegetaisUEFSBrasilDEPARTAMENTO DE CIÊNCIAS BIOLÓGICASMarcetiaFlavonoidesAtividade antioxidanteFotoproteção UVB/UVAAntioxidant activitiePhotoprotection UVB/UVA.MarcetiaFlavonoidesCIENCIAS BIOLOGICAS::BIOLOGIA GERALAvaliação da atividade fotoprotetora in vitro de extratos etanólicos de três espécies de Marcetia e suas formulaçõesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis2074743607827725103600600600-6971480722008537872-1634559385931244697info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEFSinstname:Universidade Estadual de Feira de Santana (UEFS)instacron:UEFSORIGINALTESE SÔNIA CARINE COVA COSTA.pdfTESE SÔNIA CARINE COVA COSTA.pdfapplication/pdf4450540http://tede2.uefs.br:8080/bitstream/tede/339/2/TESE+S%C3%94NIA+CARINE+COVA+COSTA.pdf273e33d63aa35b23e7686b53ad4e5b56MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://tede2.uefs.br:8080/bitstream/tede/339/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/3392016-06-16 20:04:18.379oai:tede2.uefs.br:8080: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.uefs.br:8080/PUBhttp://tede2.uefs.br:8080/oai/requestbcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.bropendoar:2016-06-16T23:04:18Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS)false |
dc.title.por.fl_str_mv |
Avaliação da atividade fotoprotetora in vitro de extratos etanólicos de três espécies de Marcetia e suas formulações |
title |
Avaliação da atividade fotoprotetora in vitro de extratos etanólicos de três espécies de Marcetia e suas formulações |
spellingShingle |
Avaliação da atividade fotoprotetora in vitro de extratos etanólicos de três espécies de Marcetia e suas formulações Costa, Sônia Carine Cova Marcetia Flavonoides Atividade antioxidante Fotoproteção UVB/UVA Antioxidant activitie Photoprotection UVB/UVA. Marcetia Flavonoides CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
title_short |
Avaliação da atividade fotoprotetora in vitro de extratos etanólicos de três espécies de Marcetia e suas formulações |
title_full |
Avaliação da atividade fotoprotetora in vitro de extratos etanólicos de três espécies de Marcetia e suas formulações |
title_fullStr |
Avaliação da atividade fotoprotetora in vitro de extratos etanólicos de três espécies de Marcetia e suas formulações |
title_full_unstemmed |
Avaliação da atividade fotoprotetora in vitro de extratos etanólicos de três espécies de Marcetia e suas formulações |
title_sort |
Avaliação da atividade fotoprotetora in vitro de extratos etanólicos de três espécies de Marcetia e suas formulações |
author |
Costa, Sônia Carine Cova |
author_facet |
Costa, Sônia Carine Cova |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Branco, Alexsandro |
dc.contributor.authorID.fl_str_mv |
94820635549 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/4166810899152932 |
dc.contributor.author.fl_str_mv |
Costa, Sônia Carine Cova |
contributor_str_mv |
Branco, Alexsandro |
dc.subject.por.fl_str_mv |
Marcetia Flavonoides Atividade antioxidante Fotoproteção UVB/UVA |
topic |
Marcetia Flavonoides Atividade antioxidante Fotoproteção UVB/UVA Antioxidant activitie Photoprotection UVB/UVA. Marcetia Flavonoides CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
dc.subject.eng.fl_str_mv |
Antioxidant activitie Photoprotection UVB/UVA. Marcetia Flavonoides |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
description |
Solar radiation is the main factor in the appearance of skin diseases. Preventive measures against skin photoaging are necessary and one of the ways to avoid it is to use sunscreen. The genus Marcetia (Melastomataceae) is endemic of rocky fields in northeastern Brazil and are adapted to high sunlight conditions due to bioproduction of flavonoids. The initial goal of this work was to evaluate the antioxidant activities, photoprotection (UVB/UVA) and toxicity of ethanolic extracts of M. taxifolia, M. macrophylla and M. latifolia. Thus, it was investigated the potential photoprotection of the extracts incorporated at 5, 10, 20 and 30% by polawax® lotion. All tests were performed in vitro. The formulations were evaluated in the physicochemical standpoint. In addition it was held HPLC-DAD for identification and quantification of flavonoids. The spectrophotometric method was used to determine the total level of flavonoids (TFC), DPPH• (antioxidant activity) and photoprotective activity (UVB/UVA). The evaluation of toxicity was made by means of the HET-CAM test, and hemolysis rate. The most promising extract was fractionated. Photoprotective formulations have been developed (5, 10, 20 and 30%), which also had their sunscreen profile UVB / UVA evaluated. The M. taxifolia showed higher quercetin content, with 55.78 g/Kg and 168 g/mL, following the studies of HPLC-DAD and TFT. All Marcetia ssp extracts had an antioxidant activity around 95%. Likewise, the three species had good results to SPF and UVA photoprotection. The species that has better value SPF was M. macrophylla (20.25) and UVA protection was M. taxifolia (86.04%), but these species have a higher toxic potential related to M. latifolia, which also had a good value SPF (13.12) and UVA (78.09%). The toxicity results show that this species is more secure and is considered an irritant and of weak hemolysis rate (23.51%), while the other species had a hemolysis rate 50%. Given these results EEML was fractionated into five fractions (MLFR02, MLFR03, MLFR04, MLFR05, MLFR06). After testing, MLFR02 showed good UVB sunscreen activity (17,52) and UVA (87.49%), but the safest was MLFR02 beyond presenting results in a high SPF value (37.82) when incorporated into the emulsion which makes it suitable for individuals who are very sensitive to sunburn. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-12-17 |
dc.date.accessioned.fl_str_mv |
2016-06-14T22:48:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
COSTA, Sônia Carine Cova. Avaliação da atividade fotoprotetora in vitro de extratos etanólicos de três espécies de Marcetia e suas formulações. 2015. 215 f. Tese (Doutorado Acadêmico em Recursos Genéticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2015. |
dc.identifier.uri.fl_str_mv |
http://localhost:8080/tede/handle/tede/339 |
identifier_str_mv |
COSTA, Sônia Carine Cova. Avaliação da atividade fotoprotetora in vitro de extratos etanólicos de três espécies de Marcetia e suas formulações. 2015. 215 f. Tese (Doutorado Acadêmico em Recursos Genéticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2015. |
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http://localhost:8080/tede/handle/tede/339 |
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por |
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por |
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600 600 600 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidade Estadual de Feira de Santana |
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Doutorado Acadêmico em Recursos Genéticos Vegetais |
dc.publisher.initials.fl_str_mv |
UEFS |
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Brasil |
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DEPARTAMENTO DE CIÊNCIAS BIOLÓGICAS |
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Universidade Estadual de Feira de Santana |
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