Estudo fitoquímico e incorporação do extrato etanólico de hymenaea martiana hayne (fabaceae) em uma formulação fitocosmética
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UEFS |
Texto Completo: | http://tede2.uefs.br:8080/handle/tede/877 |
Resumo: | Hymenaea martiana, known as “jatobá”, has great phytochemical potential, but the development of pharmaceutical or cosmetic products with this species has not been reported yet.Given this context, this study aimed to develop a phytochemical study of extract of the barks of Hymenaea martiana and incorporate the extract in a phytocosmeticformulation with photoprotective action. For this, the barks were subjected to accelerated solventextraction with ethanol, the crude extract (HM-BSE) being concentrated and partitioned.In the phytochemical study, total phenolic and flavonoid compounds were determined, in addition to photoprotective, antioxidant and antimicrobial activitiesin vitro.An analysis by HPLC-MS and a chromatographic profile in HPLC-DAD was performed with the Hm-Fr.AcOEt fraction.Three flavonoids were isolated by Sephadex® and identified by NMR.A technological application has been demonstrated with the incorporation of the extract and a chemical filter on a cosmetic basis.In the phytochemical study, Hm-EEB and the Hm-Fr.AcOEt fraction presented better dosing results with significant photoprotective, antimicrobial and antioxidant activity, with a strong correlation between activities and phenolic compounds.By CLAE-DAD, 18 bioactive flavonoids were identified.The chromatographic profile presented four major flavonoids with DAD-UV spectrum suggestive of astilbin and its isomers, and three of them were identified by NMR as neoastilbin, astilbina and isoastilbin.In the incorporation step, a synergistic effect was observed between the extract and the chemical filter, and the F4 formulation was considered a promising product.The results show the cosmetic potential of Hymenaea martiana, a native plant from the Brazilian northeast with great biotechnological and therapeutic potential |
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Silva, Jackson Roberto Guedes03016512483http://lattes.cnpq.br/676274200266025105927533442http://lattes.cnpq.br/5611320885094082Oliveira, Fernanda Granja da Silva2019-09-16T19:45:32Z2019-06-13OLIVEIRA. Fernanda Granja da Silva. Estudo fitoquímico e incorporação do extrato etanólico de hymenaea martiana hayne (fabaceae) em uma formulação fitocosmética. 2019. Tese (Doutorado Acadêmico em Biotecnologia) - Universidade Estadual de Feira de Santana, Feira de Santana, 2019.http://tede2.uefs.br:8080/handle/tede/877Hymenaea martiana, known as “jatobá”, has great phytochemical potential, but the development of pharmaceutical or cosmetic products with this species has not been reported yet.Given this context, this study aimed to develop a phytochemical study of extract of the barks of Hymenaea martiana and incorporate the extract in a phytocosmeticformulation with photoprotective action. For this, the barks were subjected to accelerated solventextraction with ethanol, the crude extract (HM-BSE) being concentrated and partitioned.In the phytochemical study, total phenolic and flavonoid compounds were determined, in addition to photoprotective, antioxidant and antimicrobial activitiesin vitro.An analysis by HPLC-MS and a chromatographic profile in HPLC-DAD was performed with the Hm-Fr.AcOEt fraction.Three flavonoids were isolated by Sephadex® and identified by NMR.A technological application has been demonstrated with the incorporation of the extract and a chemical filter on a cosmetic basis.In the phytochemical study, Hm-EEB and the Hm-Fr.AcOEt fraction presented better dosing results with significant photoprotective, antimicrobial and antioxidant activity, with a strong correlation between activities and phenolic compounds.By CLAE-DAD, 18 bioactive flavonoids were identified.The chromatographic profile presented four major flavonoids with DAD-UV spectrum suggestive of astilbin and its isomers, and three of them were identified by NMR as neoastilbin, astilbina and isoastilbin.In the incorporation step, a synergistic effect was observed between the extract and the chemical filter, and the F4 formulation was considered a promising product.The results show the cosmetic potential of Hymenaea martiana, a native plant from the Brazilian northeast with great biotechnological and therapeutic potentialHymenaea martiana, conhecida como “jatobá”, apresenta grande potencial fitoquímico, porém, ainda não foi relatado o desenvolvimento deprodutos farmacêuticos ou cosméticos com esta espécie. Diante deste contexto, este trabalho teve como objetivo realizar um estudo fitoquímico do extrato das cascas de Hymenaea martianae incorporá-lo em uma formulação fitocosmética com ação fotoprotetora. Para isso, as cascas foram submetidas à extração acelerada por solvente com etanol, sendo o extrato bruto (HM-EEB) concentrado e particionado. No estudo fitoquímico, foram determinados os compostos fenólicos e flavonoides totais, além das atividades fotoprotetora, antioxidante e antimicrobiana in vitro. Uma análise por CLAE-EM e um perfil cromatográfico em CLAE-DAD foi realizada com o extrato bruto e a fração Hm-Fr.AcOEt. Três flavonoides foram isolados por Sephadex® e identificados por RMN. Uma aplicação tecnológica foi demonstrada com a incorporação do extrato e um filtro químico em uma base cosmética. No estudo fitoquímico, o extrato bruto e a fração acetato apresentaram melhores resultados de doseamento, com atividade fotoprotetora, antimicrobiana e antioxidante significativas, com forte correlação entre as atividades e os compostos fenólicos. Por CLAE-DAD, 18 flavonoides bioativos foram identificados. O perfil cromatográfico apresentou quatro flavonoides majoritários com espectro DAD-UV sugestivo para astilbina e seus isômeros, e três desses foram identificados por RMN como neoastibilna, astilbina e isoastilbina.Na etapa de incorporação, foi observado um possível efeito sinérgico entre o extrato e o filtro químico, e a formulação F4 foi considerada um produto promissor. Os resultados evidenciam o potencial cosmético de Hymenaea martiana, uma planta nativa do nordeste brasileiro com um grande potencial biotecnológico e terapêuticoSubmitted by Verena Pereira (verenagoncalves@uefs.br) on 2019-09-16T19:45:32Z No. of bitstreams: 1 Tese Fernanda Granja PPGBiotec UEFS.pdf: 5277583 bytes, checksum: a99661838750d59a71a44bfbe333bafb (MD5)Made available in DSpace on 2019-09-16T19:45:32Z (GMT). No. of bitstreams: 1 Tese Fernanda Granja PPGBiotec UEFS.pdf: 5277583 bytes, checksum: a99661838750d59a71a44bfbe333bafb (MD5) Previous issue date: 2019-06-13Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Estadual de Feira de SantanaDoutorado Acadêmico em BiotecnologiaUEFSBrasilDEPARTAMENTO DE CIÊNCIAS BIOLÓGICASCosméticos naturaisFotoprotetores naturaisFlavonoidesFiltro SolarProdutos naturaisNatural cosmeticsNatural photoprotectivesFlavonoids.SunscreenNatural productsCIENCIAS BIOLOGICAS::FARMACOLOGIACIENCIAS BIOLOGICASEstudo fitoquímico e incorporação do extrato etanólico de hymenaea martiana hayne (fabaceae) em uma formulação fitocosméticainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis-68152692297897915436006006006006005026123383450589282700814650651154363-34391788430682021613590462550136975366info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEFSinstname:Universidade Estadual de Feira de Santana (UEFS)instacron:UEFSORIGINALTese Fernanda Granja PPGBiotec UEFS.pdfTese Fernanda Granja PPGBiotec UEFS.pdfapplication/pdf5277583http://tede2.uefs.br:8080/bitstream/tede/877/2/Tese+Fernanda+Granja+PPGBiotec+UEFS.pdfa99661838750d59a71a44bfbe333bafbMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82089http://tede2.uefs.br:8080/bitstream/tede/877/1/license.txt7b5ba3d2445355f386edab96125d42b7MD51tede/8772019-09-16 16:45:32.255oai:tede2.uefs.br:8080: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.uefs.br:8080/PUBhttp://tede2.uefs.br:8080/oai/requestbcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.bropendoar:2019-09-16T19:45:32Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS)false |
dc.title.por.fl_str_mv |
Estudo fitoquímico e incorporação do extrato etanólico de hymenaea martiana hayne (fabaceae) em uma formulação fitocosmética |
title |
Estudo fitoquímico e incorporação do extrato etanólico de hymenaea martiana hayne (fabaceae) em uma formulação fitocosmética |
spellingShingle |
Estudo fitoquímico e incorporação do extrato etanólico de hymenaea martiana hayne (fabaceae) em uma formulação fitocosmética Oliveira, Fernanda Granja da Silva Cosméticos naturais Fotoprotetores naturais Flavonoides Filtro Solar Produtos naturais Natural cosmetics Natural photoprotectives Flavonoids.Sunscreen Natural products CIENCIAS BIOLOGICAS::FARMACOLOGIA CIENCIAS BIOLOGICAS |
title_short |
Estudo fitoquímico e incorporação do extrato etanólico de hymenaea martiana hayne (fabaceae) em uma formulação fitocosmética |
title_full |
Estudo fitoquímico e incorporação do extrato etanólico de hymenaea martiana hayne (fabaceae) em uma formulação fitocosmética |
title_fullStr |
Estudo fitoquímico e incorporação do extrato etanólico de hymenaea martiana hayne (fabaceae) em uma formulação fitocosmética |
title_full_unstemmed |
Estudo fitoquímico e incorporação do extrato etanólico de hymenaea martiana hayne (fabaceae) em uma formulação fitocosmética |
title_sort |
Estudo fitoquímico e incorporação do extrato etanólico de hymenaea martiana hayne (fabaceae) em uma formulação fitocosmética |
author |
Oliveira, Fernanda Granja da Silva |
author_facet |
Oliveira, Fernanda Granja da Silva |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Silva, Jackson Roberto Guedes |
dc.contributor.advisor1ID.fl_str_mv |
03016512483 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6762742002660251 |
dc.contributor.authorID.fl_str_mv |
05927533442 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5611320885094082 |
dc.contributor.author.fl_str_mv |
Oliveira, Fernanda Granja da Silva |
contributor_str_mv |
Silva, Jackson Roberto Guedes |
dc.subject.por.fl_str_mv |
Cosméticos naturais Fotoprotetores naturais Flavonoides Filtro Solar Produtos naturais Natural cosmetics Natural photoprotectives Flavonoids.Sunscreen Natural products |
topic |
Cosméticos naturais Fotoprotetores naturais Flavonoides Filtro Solar Produtos naturais Natural cosmetics Natural photoprotectives Flavonoids.Sunscreen Natural products CIENCIAS BIOLOGICAS::FARMACOLOGIA CIENCIAS BIOLOGICAS |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::FARMACOLOGIA CIENCIAS BIOLOGICAS |
description |
Hymenaea martiana, known as “jatobá”, has great phytochemical potential, but the development of pharmaceutical or cosmetic products with this species has not been reported yet.Given this context, this study aimed to develop a phytochemical study of extract of the barks of Hymenaea martiana and incorporate the extract in a phytocosmeticformulation with photoprotective action. For this, the barks were subjected to accelerated solventextraction with ethanol, the crude extract (HM-BSE) being concentrated and partitioned.In the phytochemical study, total phenolic and flavonoid compounds were determined, in addition to photoprotective, antioxidant and antimicrobial activitiesin vitro.An analysis by HPLC-MS and a chromatographic profile in HPLC-DAD was performed with the Hm-Fr.AcOEt fraction.Three flavonoids were isolated by Sephadex® and identified by NMR.A technological application has been demonstrated with the incorporation of the extract and a chemical filter on a cosmetic basis.In the phytochemical study, Hm-EEB and the Hm-Fr.AcOEt fraction presented better dosing results with significant photoprotective, antimicrobial and antioxidant activity, with a strong correlation between activities and phenolic compounds.By CLAE-DAD, 18 bioactive flavonoids were identified.The chromatographic profile presented four major flavonoids with DAD-UV spectrum suggestive of astilbin and its isomers, and three of them were identified by NMR as neoastilbin, astilbina and isoastilbin.In the incorporation step, a synergistic effect was observed between the extract and the chemical filter, and the F4 formulation was considered a promising product.The results show the cosmetic potential of Hymenaea martiana, a native plant from the Brazilian northeast with great biotechnological and therapeutic potential |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-09-16T19:45:32Z |
dc.date.issued.fl_str_mv |
2019-06-13 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
OLIVEIRA. Fernanda Granja da Silva. Estudo fitoquímico e incorporação do extrato etanólico de hymenaea martiana hayne (fabaceae) em uma formulação fitocosmética. 2019. Tese (Doutorado Acadêmico em Biotecnologia) - Universidade Estadual de Feira de Santana, Feira de Santana, 2019. |
dc.identifier.uri.fl_str_mv |
http://tede2.uefs.br:8080/handle/tede/877 |
identifier_str_mv |
OLIVEIRA. Fernanda Granja da Silva. Estudo fitoquímico e incorporação do extrato etanólico de hymenaea martiana hayne (fabaceae) em uma formulação fitocosmética. 2019. Tese (Doutorado Acadêmico em Biotecnologia) - Universidade Estadual de Feira de Santana, Feira de Santana, 2019. |
url |
http://tede2.uefs.br:8080/handle/tede/877 |
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por |
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por |
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600 600 600 600 600 |
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openAccess |
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Universidade Estadual de Feira de Santana |
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UEFS |
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DEPARTAMENTO DE CIÊNCIAS BIOLÓGICAS |
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Universidade Estadual de Feira de Santana |
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