Light sources and culture media in the in vitro growth of the Brazilian orchid Microlaelia lundii

Detalhes bibliográficos
Autor(a) principal: Favetta, Vanessa
Data de Publicação: 2017
Outros Autores: Colombo, Ronan Carlos, Mangili Júnior, José Fernando, Faria, Ricardo Tadeu de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/26482
Resumo: The Brazilian micro-orchid Microlaelia lundii enchants by its beautiful blooms in winter, but its cultivation is still a challenge, requiring specific care. The objectiv of this work was to evaluate the influence of light sources and culture media on in vitro growth and ex vitro survival of this micro-orchid. M. lundii seedlings were transferred to flasks containing the culture medium ½ MS and Simplified Medium, consisting of 5 mL L-1 of Biofert Plus® NPK fertilizer (09-08-09) and 60 g L-1 of ‘Nanica’ banana pulp. These flasks were placed in a growth chamber composed of seven environments, characterized by types of lighting: L1) LEDtube 4000K; L2) LEDtube 6500K; L3) L1 + L2; L4) L1 + L1; L5) red LED; L6) blue LED; L7) fluorescent lamp (control). After 200 days of cultivation, biometric growth parameters and leaves chlorophyll content were evaluated together with the percentage of seedling survival when acclimatized. At the end of the experiment, an analysis of individual variances and a combined analysis of the environments were carried out. The simplified medium led to higher seedlings, especially for light source L6. Light sources L1, L2, L3 and L4 promoted an increase in the seedlings root system in the simplified medium. As for the chlorophyll content, L4 and L7 showed higher contents of chlorophyll a and b. The simplified medium led practically to 100% of seedling survival, regardless of the light source used. Results shown that the simplified medium for light sources L1, L2, L3 and L4 is recommended for the in vitro propagation of M. lundii.
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spelling Light sources and culture media in the in vitro growth of the Brazilian orchid Microlaelia lundiiFontes de luz e meios de cultura no crescimento in vitro da orquidea brasileira Microlaelia lundiiLEDNutritious mediumOrchidaceaeQuality of light.LEDMeio nutritivoOrchidaceaeQualidade da luz.The Brazilian micro-orchid Microlaelia lundii enchants by its beautiful blooms in winter, but its cultivation is still a challenge, requiring specific care. The objectiv of this work was to evaluate the influence of light sources and culture media on in vitro growth and ex vitro survival of this micro-orchid. M. lundii seedlings were transferred to flasks containing the culture medium ½ MS and Simplified Medium, consisting of 5 mL L-1 of Biofert Plus® NPK fertilizer (09-08-09) and 60 g L-1 of ‘Nanica’ banana pulp. These flasks were placed in a growth chamber composed of seven environments, characterized by types of lighting: L1) LEDtube 4000K; L2) LEDtube 6500K; L3) L1 + L2; L4) L1 + L1; L5) red LED; L6) blue LED; L7) fluorescent lamp (control). After 200 days of cultivation, biometric growth parameters and leaves chlorophyll content were evaluated together with the percentage of seedling survival when acclimatized. At the end of the experiment, an analysis of individual variances and a combined analysis of the environments were carried out. The simplified medium led to higher seedlings, especially for light source L6. Light sources L1, L2, L3 and L4 promoted an increase in the seedlings root system in the simplified medium. As for the chlorophyll content, L4 and L7 showed higher contents of chlorophyll a and b. The simplified medium led practically to 100% of seedling survival, regardless of the light source used. Results shown that the simplified medium for light sources L1, L2, L3 and L4 is recommended for the in vitro propagation of M. lundii.A micro-orquídea brasileira Microlaelia lundii encanta pelas belas floradas no inverno, no entanto o seu cultivo ainda é um desafio, requerendo cuidados específicos. Assim, objetivou-se avaliar a influência de fontes de luz e meios de cultura no crescimento in vitro e na sobrevivência ex vitro dessa micro-orquídea. Plântulas de M. lundii foram transferidas para frascos contendo os meios de cultura: ½ MS e Meio Simplificado, composto por 5 mL L-1 do fertilizante Biofert Plus® NPK (08-09-09) e 60 g L-1 de polpa de banana ‘Nanica’. Estes frascos foram acondicionados em câmara de cultivo composta por sete ambientes, caracterizados pelo tipo de iluminação: L1) LEDtube 4000K; L2) LEDtube 6500K; L3) L1 + L2; L4) L1 + L1; L5) LED vermelho; L6) LED azul; L7) Lâmpada fluorescente (controle). Após 200 dias de cultivo, avaliou-se o crescimento da parte aérea e do sistema radicular das plantas e o teor de clorofila nas folhas. Também, avaliou-se a porcentagem de sobrevivência das plantas, quando aclimatizadas. O meio simplificado propiciou plântulas mais altas, com destaque para a fonte de luz L6. As fontes de luz L1, L2, L3 e L4 no meio simplificado proporcionaram aumento do sistema radicular das plantas. Quanto ao teor de clorofila, L4 e L7 apresentaram maiores teores de clorofila a e b. O meio simplificado propiciou, praticamente, 100% de sobrevivência das plantas, independente da fonte de luz utilizada. Conclui-se que o meio simplificado nas fontes de luz L1, L2, L3 e L4 é recomendado para a propagação in vitro de M. lundii.UEL2017-08-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2648210.5433/1679-0359.2017v38n4p1775Semina: Ciências Agrárias; Vol. 38 No. 4 (2017); 1775-1784Semina: Ciências Agrárias; v. 38 n. 4 (2017); 1775-17841679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/26482/21433Copyright (c) 2017 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessFavetta, VanessaColombo, Ronan CarlosMangili Júnior, José FernandoFaria, Ricardo Tadeu de2022-10-24T12:13:59Zoai:ojs.pkp.sfu.ca:article/26482Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-24T12:13:59Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Light sources and culture media in the in vitro growth of the Brazilian orchid Microlaelia lundii
Fontes de luz e meios de cultura no crescimento in vitro da orquidea brasileira Microlaelia lundii
title Light sources and culture media in the in vitro growth of the Brazilian orchid Microlaelia lundii
spellingShingle Light sources and culture media in the in vitro growth of the Brazilian orchid Microlaelia lundii
Favetta, Vanessa
LED
Nutritious medium
Orchidaceae
Quality of light.
LED
Meio nutritivo
Orchidaceae
Qualidade da luz.
title_short Light sources and culture media in the in vitro growth of the Brazilian orchid Microlaelia lundii
title_full Light sources and culture media in the in vitro growth of the Brazilian orchid Microlaelia lundii
title_fullStr Light sources and culture media in the in vitro growth of the Brazilian orchid Microlaelia lundii
title_full_unstemmed Light sources and culture media in the in vitro growth of the Brazilian orchid Microlaelia lundii
title_sort Light sources and culture media in the in vitro growth of the Brazilian orchid Microlaelia lundii
author Favetta, Vanessa
author_facet Favetta, Vanessa
Colombo, Ronan Carlos
Mangili Júnior, José Fernando
Faria, Ricardo Tadeu de
author_role author
author2 Colombo, Ronan Carlos
Mangili Júnior, José Fernando
Faria, Ricardo Tadeu de
author2_role author
author
author
dc.contributor.author.fl_str_mv Favetta, Vanessa
Colombo, Ronan Carlos
Mangili Júnior, José Fernando
Faria, Ricardo Tadeu de
dc.subject.por.fl_str_mv LED
Nutritious medium
Orchidaceae
Quality of light.
LED
Meio nutritivo
Orchidaceae
Qualidade da luz.
topic LED
Nutritious medium
Orchidaceae
Quality of light.
LED
Meio nutritivo
Orchidaceae
Qualidade da luz.
description The Brazilian micro-orchid Microlaelia lundii enchants by its beautiful blooms in winter, but its cultivation is still a challenge, requiring specific care. The objectiv of this work was to evaluate the influence of light sources and culture media on in vitro growth and ex vitro survival of this micro-orchid. M. lundii seedlings were transferred to flasks containing the culture medium ½ MS and Simplified Medium, consisting of 5 mL L-1 of Biofert Plus® NPK fertilizer (09-08-09) and 60 g L-1 of ‘Nanica’ banana pulp. These flasks were placed in a growth chamber composed of seven environments, characterized by types of lighting: L1) LEDtube 4000K; L2) LEDtube 6500K; L3) L1 + L2; L4) L1 + L1; L5) red LED; L6) blue LED; L7) fluorescent lamp (control). After 200 days of cultivation, biometric growth parameters and leaves chlorophyll content were evaluated together with the percentage of seedling survival when acclimatized. At the end of the experiment, an analysis of individual variances and a combined analysis of the environments were carried out. The simplified medium led to higher seedlings, especially for light source L6. Light sources L1, L2, L3 and L4 promoted an increase in the seedlings root system in the simplified medium. As for the chlorophyll content, L4 and L7 showed higher contents of chlorophyll a and b. The simplified medium led practically to 100% of seedling survival, regardless of the light source used. Results shown that the simplified medium for light sources L1, L2, L3 and L4 is recommended for the in vitro propagation of M. lundii.
publishDate 2017
dc.date.none.fl_str_mv 2017-08-04
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/26482
10.5433/1679-0359.2017v38n4p1775
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/26482
identifier_str_mv 10.5433/1679-0359.2017v38n4p1775
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/26482/21433
dc.rights.driver.fl_str_mv Copyright (c) 2017 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 38 No. 4 (2017); 1775-1784
Semina: Ciências Agrárias; v. 38 n. 4 (2017); 1775-1784
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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