The effect of the synthetic oviductal fluid medium (SOF) supplemented with insulin-like growth factor-I (IGF-I) on in vitro maturation of canine oocytes (Canis familiaris)

Detalhes bibliográficos
Autor(a) principal: Machado, Marco Antonio
Data de Publicação: 2007
Outros Autores: Toniollo, Gilson Hélio, Oliveira, Kellen
Tipo de documento: Artigo
Idioma: por
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2969
Resumo: The follicular growth and oocyte maturation knowledge are very important to the development and improvement of new biotechnologies such as in vitro fertilization and somatic cell nuclear transfer. In order to the necessity of clarify the basic mechanisms related to canine oocyte maturation, this investigation focuses on the evaluation of the effect of insulin-like growth factor-i (IGF-I), added to synthetic oviductal fluid medium (SOF) on the in vitro maturation of domestic dog oocytes. Thirty-seven bitches undergoing ovariohysterectomy for castration or due to pathological conditions of the uterus were selected as oocytes’ donors (n=875). The oocytes were allocated in the following groups: M0 (stained in the collection’s time), Control (72h in SOF) and Experimental (72h in SOF plus 100 ng IGF-I). After 72 hours of maturation the oocytes’ nuclear status were assessed by Hoechst 33342 dye. The best results in terms of oocyte harvest were observed in those juvenile donors, females in estrus, nuliparous and pure breeds. No significant differences were observed between treatments control (SOF) or experimental (IGF-I).
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spelling The effect of the synthetic oviductal fluid medium (SOF) supplemented with insulin-like growth factor-I (IGF-I) on in vitro maturation of canine oocytes (Canis familiaris)Influência do fator de crescimento semelhante à insulina I (IGF-I) adicionado ao meio fluido sintético de tuba uterina (SOF) sobre a maturação in vitro de oócitos caninos (Canis familiaris)IVMNuclear maturationOocyteBitchIGF-ISOF.Maturação in vitroOócitoCadelaIGF-ISOFMaturação nuclear.The follicular growth and oocyte maturation knowledge are very important to the development and improvement of new biotechnologies such as in vitro fertilization and somatic cell nuclear transfer. In order to the necessity of clarify the basic mechanisms related to canine oocyte maturation, this investigation focuses on the evaluation of the effect of insulin-like growth factor-i (IGF-I), added to synthetic oviductal fluid medium (SOF) on the in vitro maturation of domestic dog oocytes. Thirty-seven bitches undergoing ovariohysterectomy for castration or due to pathological conditions of the uterus were selected as oocytes’ donors (n=875). The oocytes were allocated in the following groups: M0 (stained in the collection’s time), Control (72h in SOF) and Experimental (72h in SOF plus 100 ng IGF-I). After 72 hours of maturation the oocytes’ nuclear status were assessed by Hoechst 33342 dye. The best results in terms of oocyte harvest were observed in those juvenile donors, females in estrus, nuliparous and pure breeds. No significant differences were observed between treatments control (SOF) or experimental (IGF-I).O conhecimento da regulação do crescimento folicular e da maturação oocitária é de grande importância no desenvolvimento e aperfeiçoamento de novas biotecnologias como a fecundação in vitro e a transferência nuclear. Considerando-se a necessidade de elucidação dos mecanismos básicos envolvidos na maturação oocitária na espécie canina, a presente pesquisa foi desenvolvida com o objetivo de avaliar o efeito do fator de crescimento semelhante à insulina-I (IGF-I), adicionado ao meio fluido sintético de tuba uterina (SOF), sobre a maturação de oócitos caninos. Foram utilizadas 37 cadelas submetidas à ovariohisterectomia, eletivas e terapêuticas, como doadoras dos complexos cumulus oócitos grau 1 (n=875) que foram alocados em três grupos: M0 (coloração no momento da colheita), Controle (72 h no meio SOF) e Experimental (72h no meio SOF + 100 ng de IGF-I). Após 72 horas de maturação dos oócitos o estádio de maturação nuclear foi avaliado, por meio de coloração com Hoechst 33342. Os maiores índices de recuperação das estruturas foram obtidos daquelas doadoras com raças definidas, fêmeas jovens, nulíparas e em estro. Não houve diferenças estatísticas entre os dados analisados quanto à maturação nuclear entre os grupos controle (SOF) e experimental (IGF-I).UEL2007-08-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/296910.5433/1679-0359.2007v28n3p455Semina: Ciências Agrárias; Vol. 28 No. 3 (2007); 455-464Semina: Ciências Agrárias; v. 28 n. 3 (2007); 455-4641679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2969/2515Machado, Marco AntonioToniollo, Gilson HélioOliveira, Kelleninfo:eu-repo/semantics/openAccess2015-11-19T18:38:57Zoai:ojs.pkp.sfu.ca:article/2969Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2015-11-19T18:38:57Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv The effect of the synthetic oviductal fluid medium (SOF) supplemented with insulin-like growth factor-I (IGF-I) on in vitro maturation of canine oocytes (Canis familiaris)
Influência do fator de crescimento semelhante à insulina I (IGF-I) adicionado ao meio fluido sintético de tuba uterina (SOF) sobre a maturação in vitro de oócitos caninos (Canis familiaris)
title The effect of the synthetic oviductal fluid medium (SOF) supplemented with insulin-like growth factor-I (IGF-I) on in vitro maturation of canine oocytes (Canis familiaris)
spellingShingle The effect of the synthetic oviductal fluid medium (SOF) supplemented with insulin-like growth factor-I (IGF-I) on in vitro maturation of canine oocytes (Canis familiaris)
Machado, Marco Antonio
IVM
Nuclear maturation
Oocyte
Bitch
IGF-I
SOF.
Maturação in vitro
Oócito
Cadela
IGF-I
SOF
Maturação nuclear.
title_short The effect of the synthetic oviductal fluid medium (SOF) supplemented with insulin-like growth factor-I (IGF-I) on in vitro maturation of canine oocytes (Canis familiaris)
title_full The effect of the synthetic oviductal fluid medium (SOF) supplemented with insulin-like growth factor-I (IGF-I) on in vitro maturation of canine oocytes (Canis familiaris)
title_fullStr The effect of the synthetic oviductal fluid medium (SOF) supplemented with insulin-like growth factor-I (IGF-I) on in vitro maturation of canine oocytes (Canis familiaris)
title_full_unstemmed The effect of the synthetic oviductal fluid medium (SOF) supplemented with insulin-like growth factor-I (IGF-I) on in vitro maturation of canine oocytes (Canis familiaris)
title_sort The effect of the synthetic oviductal fluid medium (SOF) supplemented with insulin-like growth factor-I (IGF-I) on in vitro maturation of canine oocytes (Canis familiaris)
author Machado, Marco Antonio
author_facet Machado, Marco Antonio
Toniollo, Gilson Hélio
Oliveira, Kellen
author_role author
author2 Toniollo, Gilson Hélio
Oliveira, Kellen
author2_role author
author
dc.contributor.author.fl_str_mv Machado, Marco Antonio
Toniollo, Gilson Hélio
Oliveira, Kellen
dc.subject.por.fl_str_mv IVM
Nuclear maturation
Oocyte
Bitch
IGF-I
SOF.
Maturação in vitro
Oócito
Cadela
IGF-I
SOF
Maturação nuclear.
topic IVM
Nuclear maturation
Oocyte
Bitch
IGF-I
SOF.
Maturação in vitro
Oócito
Cadela
IGF-I
SOF
Maturação nuclear.
description The follicular growth and oocyte maturation knowledge are very important to the development and improvement of new biotechnologies such as in vitro fertilization and somatic cell nuclear transfer. In order to the necessity of clarify the basic mechanisms related to canine oocyte maturation, this investigation focuses on the evaluation of the effect of insulin-like growth factor-i (IGF-I), added to synthetic oviductal fluid medium (SOF) on the in vitro maturation of domestic dog oocytes. Thirty-seven bitches undergoing ovariohysterectomy for castration or due to pathological conditions of the uterus were selected as oocytes’ donors (n=875). The oocytes were allocated in the following groups: M0 (stained in the collection’s time), Control (72h in SOF) and Experimental (72h in SOF plus 100 ng IGF-I). After 72 hours of maturation the oocytes’ nuclear status were assessed by Hoechst 33342 dye. The best results in terms of oocyte harvest were observed in those juvenile donors, females in estrus, nuliparous and pure breeds. No significant differences were observed between treatments control (SOF) or experimental (IGF-I).
publishDate 2007
dc.date.none.fl_str_mv 2007-08-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2969
10.5433/1679-0359.2007v28n3p455
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2969
identifier_str_mv 10.5433/1679-0359.2007v28n3p455
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2969/2515
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 28 No. 3 (2007); 455-464
Semina: Ciências Agrárias; v. 28 n. 3 (2007); 455-464
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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