The effect of the synthetic oviductal fluid medium (SOF) supplemented with insulin-like growth factor-I (IGF-I) on in vitro maturation of canine oocytes (Canis familiaris)
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2969 |
Resumo: | The follicular growth and oocyte maturation knowledge are very important to the development and improvement of new biotechnologies such as in vitro fertilization and somatic cell nuclear transfer. In order to the necessity of clarify the basic mechanisms related to canine oocyte maturation, this investigation focuses on the evaluation of the effect of insulin-like growth factor-i (IGF-I), added to synthetic oviductal fluid medium (SOF) on the in vitro maturation of domestic dog oocytes. Thirty-seven bitches undergoing ovariohysterectomy for castration or due to pathological conditions of the uterus were selected as oocytes’ donors (n=875). The oocytes were allocated in the following groups: M0 (stained in the collection’s time), Control (72h in SOF) and Experimental (72h in SOF plus 100 ng IGF-I). After 72 hours of maturation the oocytes’ nuclear status were assessed by Hoechst 33342 dye. The best results in terms of oocyte harvest were observed in those juvenile donors, females in estrus, nuliparous and pure breeds. No significant differences were observed between treatments control (SOF) or experimental (IGF-I). |
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Semina. Ciências Agrárias (Online) |
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The effect of the synthetic oviductal fluid medium (SOF) supplemented with insulin-like growth factor-I (IGF-I) on in vitro maturation of canine oocytes (Canis familiaris)Influência do fator de crescimento semelhante à insulina I (IGF-I) adicionado ao meio fluido sintético de tuba uterina (SOF) sobre a maturação in vitro de oócitos caninos (Canis familiaris)IVMNuclear maturationOocyteBitchIGF-ISOF.Maturação in vitroOócitoCadelaIGF-ISOFMaturação nuclear.The follicular growth and oocyte maturation knowledge are very important to the development and improvement of new biotechnologies such as in vitro fertilization and somatic cell nuclear transfer. In order to the necessity of clarify the basic mechanisms related to canine oocyte maturation, this investigation focuses on the evaluation of the effect of insulin-like growth factor-i (IGF-I), added to synthetic oviductal fluid medium (SOF) on the in vitro maturation of domestic dog oocytes. Thirty-seven bitches undergoing ovariohysterectomy for castration or due to pathological conditions of the uterus were selected as oocytes’ donors (n=875). The oocytes were allocated in the following groups: M0 (stained in the collection’s time), Control (72h in SOF) and Experimental (72h in SOF plus 100 ng IGF-I). After 72 hours of maturation the oocytes’ nuclear status were assessed by Hoechst 33342 dye. The best results in terms of oocyte harvest were observed in those juvenile donors, females in estrus, nuliparous and pure breeds. No significant differences were observed between treatments control (SOF) or experimental (IGF-I).O conhecimento da regulação do crescimento folicular e da maturação oocitária é de grande importância no desenvolvimento e aperfeiçoamento de novas biotecnologias como a fecundação in vitro e a transferência nuclear. Considerando-se a necessidade de elucidação dos mecanismos básicos envolvidos na maturação oocitária na espécie canina, a presente pesquisa foi desenvolvida com o objetivo de avaliar o efeito do fator de crescimento semelhante à insulina-I (IGF-I), adicionado ao meio fluido sintético de tuba uterina (SOF), sobre a maturação de oócitos caninos. Foram utilizadas 37 cadelas submetidas à ovariohisterectomia, eletivas e terapêuticas, como doadoras dos complexos cumulus oócitos grau 1 (n=875) que foram alocados em três grupos: M0 (coloração no momento da colheita), Controle (72 h no meio SOF) e Experimental (72h no meio SOF + 100 ng de IGF-I). Após 72 horas de maturação dos oócitos o estádio de maturação nuclear foi avaliado, por meio de coloração com Hoechst 33342. Os maiores índices de recuperação das estruturas foram obtidos daquelas doadoras com raças definidas, fêmeas jovens, nulíparas e em estro. Não houve diferenças estatísticas entre os dados analisados quanto à maturação nuclear entre os grupos controle (SOF) e experimental (IGF-I).UEL2007-08-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/296910.5433/1679-0359.2007v28n3p455Semina: Ciências Agrárias; Vol. 28 No. 3 (2007); 455-464Semina: Ciências Agrárias; v. 28 n. 3 (2007); 455-4641679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2969/2515Machado, Marco AntonioToniollo, Gilson HélioOliveira, Kelleninfo:eu-repo/semantics/openAccess2015-11-19T18:38:57Zoai:ojs.pkp.sfu.ca:article/2969Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2015-11-19T18:38:57Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
The effect of the synthetic oviductal fluid medium (SOF) supplemented with insulin-like growth factor-I (IGF-I) on in vitro maturation of canine oocytes (Canis familiaris) Influência do fator de crescimento semelhante à insulina I (IGF-I) adicionado ao meio fluido sintético de tuba uterina (SOF) sobre a maturação in vitro de oócitos caninos (Canis familiaris) |
title |
The effect of the synthetic oviductal fluid medium (SOF) supplemented with insulin-like growth factor-I (IGF-I) on in vitro maturation of canine oocytes (Canis familiaris) |
spellingShingle |
The effect of the synthetic oviductal fluid medium (SOF) supplemented with insulin-like growth factor-I (IGF-I) on in vitro maturation of canine oocytes (Canis familiaris) Machado, Marco Antonio IVM Nuclear maturation Oocyte Bitch IGF-I SOF. Maturação in vitro Oócito Cadela IGF-I SOF Maturação nuclear. |
title_short |
The effect of the synthetic oviductal fluid medium (SOF) supplemented with insulin-like growth factor-I (IGF-I) on in vitro maturation of canine oocytes (Canis familiaris) |
title_full |
The effect of the synthetic oviductal fluid medium (SOF) supplemented with insulin-like growth factor-I (IGF-I) on in vitro maturation of canine oocytes (Canis familiaris) |
title_fullStr |
The effect of the synthetic oviductal fluid medium (SOF) supplemented with insulin-like growth factor-I (IGF-I) on in vitro maturation of canine oocytes (Canis familiaris) |
title_full_unstemmed |
The effect of the synthetic oviductal fluid medium (SOF) supplemented with insulin-like growth factor-I (IGF-I) on in vitro maturation of canine oocytes (Canis familiaris) |
title_sort |
The effect of the synthetic oviductal fluid medium (SOF) supplemented with insulin-like growth factor-I (IGF-I) on in vitro maturation of canine oocytes (Canis familiaris) |
author |
Machado, Marco Antonio |
author_facet |
Machado, Marco Antonio Toniollo, Gilson Hélio Oliveira, Kellen |
author_role |
author |
author2 |
Toniollo, Gilson Hélio Oliveira, Kellen |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Machado, Marco Antonio Toniollo, Gilson Hélio Oliveira, Kellen |
dc.subject.por.fl_str_mv |
IVM Nuclear maturation Oocyte Bitch IGF-I SOF. Maturação in vitro Oócito Cadela IGF-I SOF Maturação nuclear. |
topic |
IVM Nuclear maturation Oocyte Bitch IGF-I SOF. Maturação in vitro Oócito Cadela IGF-I SOF Maturação nuclear. |
description |
The follicular growth and oocyte maturation knowledge are very important to the development and improvement of new biotechnologies such as in vitro fertilization and somatic cell nuclear transfer. In order to the necessity of clarify the basic mechanisms related to canine oocyte maturation, this investigation focuses on the evaluation of the effect of insulin-like growth factor-i (IGF-I), added to synthetic oviductal fluid medium (SOF) on the in vitro maturation of domestic dog oocytes. Thirty-seven bitches undergoing ovariohysterectomy for castration or due to pathological conditions of the uterus were selected as oocytes’ donors (n=875). The oocytes were allocated in the following groups: M0 (stained in the collection’s time), Control (72h in SOF) and Experimental (72h in SOF plus 100 ng IGF-I). After 72 hours of maturation the oocytes’ nuclear status were assessed by Hoechst 33342 dye. The best results in terms of oocyte harvest were observed in those juvenile donors, females in estrus, nuliparous and pure breeds. No significant differences were observed between treatments control (SOF) or experimental (IGF-I). |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-08-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2969 10.5433/1679-0359.2007v28n3p455 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2969 |
identifier_str_mv |
10.5433/1679-0359.2007v28n3p455 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2969/2515 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 28 No. 3 (2007); 455-464 Semina: Ciências Agrárias; v. 28 n. 3 (2007); 455-464 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306059321442304 |