Antibiotic resistance profile of gram-negative bacteria isolated from dog nasal swab samples, and antibacterial and antioxidant activities of aqueous extracts of Alpinia purpurta (Vieill.) K. Schum (Zingiberaceae)
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/39504 |
Resumo: | The indiscriminate use of antibiotics in veterinary medicine and their negligent use among dog owners have contributed to the rise of antibiotic resistance in microorganisms found in pets. In addition, the search for medicinal plants with antibacterial properties has made the evaluation of aqueous extracts of Alpinia purpurata (Vieill.) K. Schum an important issue. Thus, the aim of this work was to determine the antibiotic resistance profile of gram-negative bacteria isolated from nasal swab samples of dogs and assess the antibacterial activity of the aqueous extracts of leaves and rhizomes of A. purpurata. The bacteria identified were tested using the agar disc diffusion assay for the evaluation of antibiotic resistance. A total of 16 isolates were obtained from the 19 samples collected, with a high prevalence of Escherichia coli (n=5). There was a high rate of resistance to ?-lactams, where the highest percentage was seen for amoxicillin (72.5%). Aqueous leaf extracts had high levels of total phenolic compounds (637.47 µg GAE mg-1), differing significantly (p < 0.05) from aqueous rhizome extracts (228.64 µg GAE mg-1). There was no significant difference in EC50 of DPPH values between the aqueous extracts; however, the antioxidant capacity of rhizome extracts had higher values than leaf extracts. The minimum inhibitory concentration (MIC) of leaves and rhizomes for the evaluated bacteria ranged from 9000 to 32,000 µg mL-1. For the minimum bactericidal concentration (MBC), most bacteria showed an MBC over 38,400 µg mL-1 for the rhizome. In conclusion, the bacteria isolated from dog nasal swabs showed a high resistance profile for the antibiotics of the penicillin class. Additionally, the results from the analysis of the aqueous extracts of rhizomes and leaves of A. purpurata showed an antimicrobial effect possibly associated with a high content of total phenolic compounds; these results can create a scope for using these extracts together with conventional antibiotics to control the emergence of antibiotic resistance among microbial species. |
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Antibiotic resistance profile of gram-negative bacteria isolated from dog nasal swab samples, and antibacterial and antioxidant activities of aqueous extracts of Alpinia purpurta (Vieill.) K. Schum (Zingiberaceae)Perfil de resistência a antibióticos de bactérias gram-negativas isoladas de amostras de swab nasal de cães e atividades antibacteriana e antioxidante de extratos aquosos de Alpinia purpurta (Vieill.) K. Schum. (Zingiberaceae)AntimicrobialEscherichia coliGingerSerratia liquefaciensHafnia alveiPantoea agglomerans.AntimicrobianosEscherichia coliGengibreSerratia liquefaciensHafnia alveiPantoea agglomerans.The indiscriminate use of antibiotics in veterinary medicine and their negligent use among dog owners have contributed to the rise of antibiotic resistance in microorganisms found in pets. In addition, the search for medicinal plants with antibacterial properties has made the evaluation of aqueous extracts of Alpinia purpurata (Vieill.) K. Schum an important issue. Thus, the aim of this work was to determine the antibiotic resistance profile of gram-negative bacteria isolated from nasal swab samples of dogs and assess the antibacterial activity of the aqueous extracts of leaves and rhizomes of A. purpurata. The bacteria identified were tested using the agar disc diffusion assay for the evaluation of antibiotic resistance. A total of 16 isolates were obtained from the 19 samples collected, with a high prevalence of Escherichia coli (n=5). There was a high rate of resistance to ?-lactams, where the highest percentage was seen for amoxicillin (72.5%). Aqueous leaf extracts had high levels of total phenolic compounds (637.47 µg GAE mg-1), differing significantly (p < 0.05) from aqueous rhizome extracts (228.64 µg GAE mg-1). There was no significant difference in EC50 of DPPH values between the aqueous extracts; however, the antioxidant capacity of rhizome extracts had higher values than leaf extracts. The minimum inhibitory concentration (MIC) of leaves and rhizomes for the evaluated bacteria ranged from 9000 to 32,000 µg mL-1. For the minimum bactericidal concentration (MBC), most bacteria showed an MBC over 38,400 µg mL-1 for the rhizome. In conclusion, the bacteria isolated from dog nasal swabs showed a high resistance profile for the antibiotics of the penicillin class. Additionally, the results from the analysis of the aqueous extracts of rhizomes and leaves of A. purpurata showed an antimicrobial effect possibly associated with a high content of total phenolic compounds; these results can create a scope for using these extracts together with conventional antibiotics to control the emergence of antibiotic resistance among microbial species.O uso indiscriminado de antibióticos na Medicina Veterinária e negligência de tutores de cães no uso adequado contribuíram no aumento de resistência em animais de companhia. Além disso, a busca por plantas medicinais que tenham efeito antibacteriano tornou importante a avaliação de extratos aquosos de Alpinia purpurata (Vieill.) K. Schum. Dessa forma, o objetivo do trabalho foi determinar o perfil de resistência aos antibióticos de bactérias Gram-negativas isoladas de amostras de swabs nasais de cães e verificar a atividade antibacteriana dos extratos aquosos das folhas e dos rizomas de A. purpurata. As bactérias identificadas foram submetidas à técnica de disco difusão em ágar para avaliação da resistência aos antibióticos. Das 19 amostras colhidas, foram isoladas 16 bactérias, com maior prevalência para Escherichia coli (n=5). Verificou-se um alto índice de resistência aos ?-lactâmicos testados, com maior percentual para amoxicilina (72,5%). Os extratos aquosos das folhas apresentam elevados teores de compostos fenólicos totais (637,47 µg GAE mg-1 extrato), sendo diferente (p < 0,05) dos extratos aquosos dos rizomas (228,64 µg GAE mg-1 extrato). Não houve diferença significativa nos valores de EC50 – DPPH para os extratos aquosos, entretanto, para a capacidade antioxidante FRAP, os extratos dos rizomas de A. purpurata demonstraram maiores valores quando comparados com os extratos aquosos das folhas. A Concentração Inibitória Mínima (CIM) das folhas e rizomas para as bactérias avaliadas variaram entre 9.000 a 32.000 µg mL-1. Para a Concentração Bactericida Mínima (CBM), a maioria das bactérias apresentaram CBM acima de 38.400 µg mL-1 para o rizoma. Conclui-se que as bactérias isoladas de swab nasal de cães apresentam alto perfil de resistência aos antibióticos da classe das penicilinas, no entanto, os resultados dos extratos aquosos dos rizomas e das folhas de A. purpurata sinalizam efeito antimicrobiano possivelmente associado ao elevado teor de compostos fenólicos totais, podendo ser utilizado em conjunto aos antibióticos convencionais, reduzindo assim sua resistência.UEL2021-01-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3950410.5433/1679-0359.2021v42n1p179Semina: Ciências Agrárias; Vol. 42 No. 1 (2021); 179-192Semina: Ciências Agrárias; v. 42 n. 1 (2021); 179-1921679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/39504/28502Copyright (c) 2020 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessMatusaiki, Camila de CuffaFerreira, Rafaela GalvesOtutumi, Luciana KazueSantos, Isabela Carvalho dosRamos, Felipe André PereiraMezalira, Taniara SuelenJacomassi, EzildaBarbosa, Lidiane NunesGonçalves, Daniela DibSoares, Andréia Assunção2022-10-04T15:14:17Zoai:ojs.pkp.sfu.ca:article/39504Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-04T15:14:17Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Antibiotic resistance profile of gram-negative bacteria isolated from dog nasal swab samples, and antibacterial and antioxidant activities of aqueous extracts of Alpinia purpurta (Vieill.) K. Schum (Zingiberaceae) Perfil de resistência a antibióticos de bactérias gram-negativas isoladas de amostras de swab nasal de cães e atividades antibacteriana e antioxidante de extratos aquosos de Alpinia purpurta (Vieill.) K. Schum. (Zingiberaceae) |
title |
Antibiotic resistance profile of gram-negative bacteria isolated from dog nasal swab samples, and antibacterial and antioxidant activities of aqueous extracts of Alpinia purpurta (Vieill.) K. Schum (Zingiberaceae) |
spellingShingle |
Antibiotic resistance profile of gram-negative bacteria isolated from dog nasal swab samples, and antibacterial and antioxidant activities of aqueous extracts of Alpinia purpurta (Vieill.) K. Schum (Zingiberaceae) Matusaiki, Camila de Cuffa Antimicrobial Escherichia coli Ginger Serratia liquefaciens Hafnia alvei Pantoea agglomerans. Antimicrobianos Escherichia coli Gengibre Serratia liquefaciens Hafnia alvei Pantoea agglomerans. |
title_short |
Antibiotic resistance profile of gram-negative bacteria isolated from dog nasal swab samples, and antibacterial and antioxidant activities of aqueous extracts of Alpinia purpurta (Vieill.) K. Schum (Zingiberaceae) |
title_full |
Antibiotic resistance profile of gram-negative bacteria isolated from dog nasal swab samples, and antibacterial and antioxidant activities of aqueous extracts of Alpinia purpurta (Vieill.) K. Schum (Zingiberaceae) |
title_fullStr |
Antibiotic resistance profile of gram-negative bacteria isolated from dog nasal swab samples, and antibacterial and antioxidant activities of aqueous extracts of Alpinia purpurta (Vieill.) K. Schum (Zingiberaceae) |
title_full_unstemmed |
Antibiotic resistance profile of gram-negative bacteria isolated from dog nasal swab samples, and antibacterial and antioxidant activities of aqueous extracts of Alpinia purpurta (Vieill.) K. Schum (Zingiberaceae) |
title_sort |
Antibiotic resistance profile of gram-negative bacteria isolated from dog nasal swab samples, and antibacterial and antioxidant activities of aqueous extracts of Alpinia purpurta (Vieill.) K. Schum (Zingiberaceae) |
author |
Matusaiki, Camila de Cuffa |
author_facet |
Matusaiki, Camila de Cuffa Ferreira, Rafaela Galves Otutumi, Luciana Kazue Santos, Isabela Carvalho dos Ramos, Felipe André Pereira Mezalira, Taniara Suelen Jacomassi, Ezilda Barbosa, Lidiane Nunes Gonçalves, Daniela Dib Soares, Andréia Assunção |
author_role |
author |
author2 |
Ferreira, Rafaela Galves Otutumi, Luciana Kazue Santos, Isabela Carvalho dos Ramos, Felipe André Pereira Mezalira, Taniara Suelen Jacomassi, Ezilda Barbosa, Lidiane Nunes Gonçalves, Daniela Dib Soares, Andréia Assunção |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Matusaiki, Camila de Cuffa Ferreira, Rafaela Galves Otutumi, Luciana Kazue Santos, Isabela Carvalho dos Ramos, Felipe André Pereira Mezalira, Taniara Suelen Jacomassi, Ezilda Barbosa, Lidiane Nunes Gonçalves, Daniela Dib Soares, Andréia Assunção |
dc.subject.por.fl_str_mv |
Antimicrobial Escherichia coli Ginger Serratia liquefaciens Hafnia alvei Pantoea agglomerans. Antimicrobianos Escherichia coli Gengibre Serratia liquefaciens Hafnia alvei Pantoea agglomerans. |
topic |
Antimicrobial Escherichia coli Ginger Serratia liquefaciens Hafnia alvei Pantoea agglomerans. Antimicrobianos Escherichia coli Gengibre Serratia liquefaciens Hafnia alvei Pantoea agglomerans. |
description |
The indiscriminate use of antibiotics in veterinary medicine and their negligent use among dog owners have contributed to the rise of antibiotic resistance in microorganisms found in pets. In addition, the search for medicinal plants with antibacterial properties has made the evaluation of aqueous extracts of Alpinia purpurata (Vieill.) K. Schum an important issue. Thus, the aim of this work was to determine the antibiotic resistance profile of gram-negative bacteria isolated from nasal swab samples of dogs and assess the antibacterial activity of the aqueous extracts of leaves and rhizomes of A. purpurata. The bacteria identified were tested using the agar disc diffusion assay for the evaluation of antibiotic resistance. A total of 16 isolates were obtained from the 19 samples collected, with a high prevalence of Escherichia coli (n=5). There was a high rate of resistance to ?-lactams, where the highest percentage was seen for amoxicillin (72.5%). Aqueous leaf extracts had high levels of total phenolic compounds (637.47 µg GAE mg-1), differing significantly (p < 0.05) from aqueous rhizome extracts (228.64 µg GAE mg-1). There was no significant difference in EC50 of DPPH values between the aqueous extracts; however, the antioxidant capacity of rhizome extracts had higher values than leaf extracts. The minimum inhibitory concentration (MIC) of leaves and rhizomes for the evaluated bacteria ranged from 9000 to 32,000 µg mL-1. For the minimum bactericidal concentration (MBC), most bacteria showed an MBC over 38,400 µg mL-1 for the rhizome. In conclusion, the bacteria isolated from dog nasal swabs showed a high resistance profile for the antibiotics of the penicillin class. Additionally, the results from the analysis of the aqueous extracts of rhizomes and leaves of A. purpurata showed an antimicrobial effect possibly associated with a high content of total phenolic compounds; these results can create a scope for using these extracts together with conventional antibiotics to control the emergence of antibiotic resistance among microbial species. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-19 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/39504 10.5433/1679-0359.2021v42n1p179 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/39504 |
identifier_str_mv |
10.5433/1679-0359.2021v42n1p179 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/39504/28502 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 42 No. 1 (2021); 179-192 Semina: Ciências Agrárias; v. 42 n. 1 (2021); 179-192 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306082789621760 |