Respiratory profile and gill histopathology of Carassius auratus exposed to different salinity concentrations

Detalhes bibliográficos
Autor(a) principal: Silva, Weliton Vilhalba da
Data de Publicação: 2021
Outros Autores: Ziemniczak, Henrique Momo, Bacha, Flávia Barbieri, Fernandes, Rudã Brandão Santos, Fujimoto, Rodrigo Yudi, Sousa, Rebeca Maria, Saturnino, Klaus Casaro, Honorato, Claucia Aparecida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/41949
Resumo: The aim of this study was to evaluate the chronic salinity tolerance of Carassius auratus and the effects on blood parameters, gill morphology, and survival. In the first test, nine different concentrations (0.0, 0.5, 1.0, 2.5, 5.0, 10, 15, 20, and 25 g L-1) of NaCl were used with nine repetitions for 96 h. The survival of fish subjected to 15 g L-1 NaCl was 4 h, and 5 min at a concentration of 25 g L-1. The mortality of fish with 15 g L-1 NaCl was 100%. Morphological analyses of the gills showed hyperplasia of the coated cells in the interlamellar space and hypersecretion of mucus in fish exposed to 10 g L-1 of NaCl. At concentrations of 20 and 25 g L-1, necrosis of the support collagen caused the cells to detach from the lamellar structure mucosa. In the chronic test, two concentrations were used, with four replications containing nine fish in each aquarium for a period of 21 days. Blood samples and gills from the fish were collected, and it was observed that the fish showed a decrease in the concentration of bicarbonate (NaHCO3) in the blood, indicating hypernatremia. Acute exposure of C. auratus to sodium chloride (NaCl) should be at a maximum of 10 g L-1 of NaCl, after which level there would be a loss in animal performance and/or mortality. Chronic exposure to 5 g L-1 of NaCl promotes acidemia, ionic imbalance, and pathological changes in the gills; therefore, it is not recommended.
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spelling Respiratory profile and gill histopathology of Carassius auratus exposed to different salinity concentrationsPerfil respiratório e histopatologia das brânquias de Carassius auratus expostas a diferentes concentrações de salinidadesCommon saltConcentrationKinguioOrnamental fishOsmoregulation.Sal comumConcentraçãoKinguioOsmorregulaçãoPeixes ornamentais.The aim of this study was to evaluate the chronic salinity tolerance of Carassius auratus and the effects on blood parameters, gill morphology, and survival. In the first test, nine different concentrations (0.0, 0.5, 1.0, 2.5, 5.0, 10, 15, 20, and 25 g L-1) of NaCl were used with nine repetitions for 96 h. The survival of fish subjected to 15 g L-1 NaCl was 4 h, and 5 min at a concentration of 25 g L-1. The mortality of fish with 15 g L-1 NaCl was 100%. Morphological analyses of the gills showed hyperplasia of the coated cells in the interlamellar space and hypersecretion of mucus in fish exposed to 10 g L-1 of NaCl. At concentrations of 20 and 25 g L-1, necrosis of the support collagen caused the cells to detach from the lamellar structure mucosa. In the chronic test, two concentrations were used, with four replications containing nine fish in each aquarium for a period of 21 days. Blood samples and gills from the fish were collected, and it was observed that the fish showed a decrease in the concentration of bicarbonate (NaHCO3) in the blood, indicating hypernatremia. Acute exposure of C. auratus to sodium chloride (NaCl) should be at a maximum of 10 g L-1 of NaCl, after which level there would be a loss in animal performance and/or mortality. Chronic exposure to 5 g L-1 of NaCl promotes acidemia, ionic imbalance, and pathological changes in the gills; therefore, it is not recommended.O objetivo deste estudo foi avaliar a tolerância crônica de Carassius auratus à salinidade e os efeitos sobre os parâmetros sanguíneos, morfologia branquial e sobrevivência. No primeiro teste, foram utilizadas nove concentrações de NaCl (0.0, 0.5, 1.0, 2.5, 5.0, 10, 15, 20, e 25 g L-1) e nove repetições por 96 h. A sobrevivência dos peixes submetidos a até 15 g L-1 de NaCl foi de 04h00, sendo 00h05 na concentração de 25 g L-1 de NaCl. A mortalidade dos peixes com 15 g foi de 100%. As análises morfológicas das brânquias mostraram hiperplasia das células revestidas no espaço interlamelar e hipersecreção de muco em peixes expostos a uma concentração de 10 g L-1 de NaCl. Nas concentrações de 20 e 25 g L-1, observou-se que a colágeno de suporte perdeu a estrutura das células da mucosa, alterando as lamelas secundárias. Em um segundo experimento, um delineamento inteiramente casualizado foi utilizado com dois tratamentos (0 e 5 g L-1 de NaCl) e quatro repetições com nove peixes por 30 L em 21 dias. Amostras de sangue e brânquias dos peixes foram coletadas e observou-se que os peixes apresentaram diminuição nas concentrações de bicarbonato (NaHCO3) no sangue, indicando hipernatremia. Conclui-se que a exposição aguda de C. auratus ao cloreto de sódio (NaCl) deve ser de no máximo 10 g L-1, levando à perda de desempenho e/ou mortalidade dos animais. A exposição crônica a 5 g L-1 de NaCl promove acidemia, desequilíbrio iônico e alterações patológicas nas brânquias, por isso não é recomendado.UEL2021-07-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa Históricaapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4194910.5433/1679-0359.2021v42n5p2993Semina: Ciências Agrárias; Vol. 42 No. 5 (2021); 2993-3006Semina: Ciências Agrárias; v. 42 n. 5 (2021); 2993-30061679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/41949/29964Copyright (c) 2021 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessSilva, Weliton Vilhalba daZiemniczak, Henrique MomoBacha, Flávia BarbieriFernandes, Rudã Brandão SantosFujimoto, Rodrigo YudiSousa, Rebeca MariaSaturnino, Klaus CasaroHonorato, Claucia Aparecida2022-09-30T13:35:25Zoai:ojs.pkp.sfu.ca:article/41949Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-09-30T13:35:25Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Respiratory profile and gill histopathology of Carassius auratus exposed to different salinity concentrations
Perfil respiratório e histopatologia das brânquias de Carassius auratus expostas a diferentes concentrações de salinidades
title Respiratory profile and gill histopathology of Carassius auratus exposed to different salinity concentrations
spellingShingle Respiratory profile and gill histopathology of Carassius auratus exposed to different salinity concentrations
Silva, Weliton Vilhalba da
Common salt
Concentration
Kinguio
Ornamental fish
Osmoregulation.
Sal comum
Concentração
Kinguio
Osmorregulação
Peixes ornamentais.
title_short Respiratory profile and gill histopathology of Carassius auratus exposed to different salinity concentrations
title_full Respiratory profile and gill histopathology of Carassius auratus exposed to different salinity concentrations
title_fullStr Respiratory profile and gill histopathology of Carassius auratus exposed to different salinity concentrations
title_full_unstemmed Respiratory profile and gill histopathology of Carassius auratus exposed to different salinity concentrations
title_sort Respiratory profile and gill histopathology of Carassius auratus exposed to different salinity concentrations
author Silva, Weliton Vilhalba da
author_facet Silva, Weliton Vilhalba da
Ziemniczak, Henrique Momo
Bacha, Flávia Barbieri
Fernandes, Rudã Brandão Santos
Fujimoto, Rodrigo Yudi
Sousa, Rebeca Maria
Saturnino, Klaus Casaro
Honorato, Claucia Aparecida
author_role author
author2 Ziemniczak, Henrique Momo
Bacha, Flávia Barbieri
Fernandes, Rudã Brandão Santos
Fujimoto, Rodrigo Yudi
Sousa, Rebeca Maria
Saturnino, Klaus Casaro
Honorato, Claucia Aparecida
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Weliton Vilhalba da
Ziemniczak, Henrique Momo
Bacha, Flávia Barbieri
Fernandes, Rudã Brandão Santos
Fujimoto, Rodrigo Yudi
Sousa, Rebeca Maria
Saturnino, Klaus Casaro
Honorato, Claucia Aparecida
dc.subject.por.fl_str_mv Common salt
Concentration
Kinguio
Ornamental fish
Osmoregulation.
Sal comum
Concentração
Kinguio
Osmorregulação
Peixes ornamentais.
topic Common salt
Concentration
Kinguio
Ornamental fish
Osmoregulation.
Sal comum
Concentração
Kinguio
Osmorregulação
Peixes ornamentais.
description The aim of this study was to evaluate the chronic salinity tolerance of Carassius auratus and the effects on blood parameters, gill morphology, and survival. In the first test, nine different concentrations (0.0, 0.5, 1.0, 2.5, 5.0, 10, 15, 20, and 25 g L-1) of NaCl were used with nine repetitions for 96 h. The survival of fish subjected to 15 g L-1 NaCl was 4 h, and 5 min at a concentration of 25 g L-1. The mortality of fish with 15 g L-1 NaCl was 100%. Morphological analyses of the gills showed hyperplasia of the coated cells in the interlamellar space and hypersecretion of mucus in fish exposed to 10 g L-1 of NaCl. At concentrations of 20 and 25 g L-1, necrosis of the support collagen caused the cells to detach from the lamellar structure mucosa. In the chronic test, two concentrations were used, with four replications containing nine fish in each aquarium for a period of 21 days. Blood samples and gills from the fish were collected, and it was observed that the fish showed a decrease in the concentration of bicarbonate (NaHCO3) in the blood, indicating hypernatremia. Acute exposure of C. auratus to sodium chloride (NaCl) should be at a maximum of 10 g L-1 of NaCl, after which level there would be a loss in animal performance and/or mortality. Chronic exposure to 5 g L-1 of NaCl promotes acidemia, ionic imbalance, and pathological changes in the gills; therefore, it is not recommended.
publishDate 2021
dc.date.none.fl_str_mv 2021-07-02
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Pesquisa Histórica
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/41949
10.5433/1679-0359.2021v42n5p2993
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/41949
identifier_str_mv 10.5433/1679-0359.2021v42n5p2993
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/41949/29964
dc.rights.driver.fl_str_mv Copyright (c) 2021 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2021 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 42 No. 5 (2021); 2993-3006
Semina: Ciências Agrárias; v. 42 n. 5 (2021); 2993-3006
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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