Evaluation histological, immunofluorescence and immunohistochemistry techniques for the postmortem diagnostic of the swine toxoplasmosis.

Detalhes bibliográficos
Autor(a) principal: Viotti, Neusa Maria Alves
Data de Publicação: 1995
Outros Autores: Freire, Roberta Lemos, Navarro, Italmar Teodorico, Vidotto, Odilon
Tipo de documento: Artigo
Idioma: por
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4317
Resumo: Six 120 days old Landrace/Large-white swines with were inoculated with taquizoites of the AS 28, LIV-IV and N Toxoplasma gondii strains and analysed under clinical, sorological, parasitological and histopathological aspects. In order to evaluate some postmortem diagnostic techniques, three swines were sacrificed in the acute phase and three swines in the chronic phase of the infection. Formalin-fixed and parafin-embedded tissues were submitted to Eosin-Hematoxilin, Indirect Immunofluorescence (IFA) and Peroxidase Anti-Peroxidase (PAP) techniques. The Eosin-Hematoxilin showed to be efficient in the analysis of the morphological alterations in the tissues, but did not detect any form of the parasite. Both, IFA and PAP revealed taquizoites of T. gondii, although the last one showed to be more effective because it also allowed an analysis of the morphological tissue alterations.  
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spelling Evaluation histological, immunofluorescence and immunohistochemistry techniques for the postmortem diagnostic of the swine toxoplasmosis.Avaliação das técnicas de hematoxilina-eosina, imunofluorescência e peroxidase anti-peroxidase no diagnóstico post-mortem da toxoplasmose suínaToxoplasmosisHistopatologyImmunohistochemistryImmunofluorescenceSwineToxoplasmoseHistopatologiaImunohistoquímicaImunofluorescênciaSuínos. Six 120 days old Landrace/Large-white swines with were inoculated with taquizoites of the AS 28, LIV-IV and N Toxoplasma gondii strains and analysed under clinical, sorological, parasitological and histopathological aspects. In order to evaluate some postmortem diagnostic techniques, three swines were sacrificed in the acute phase and three swines in the chronic phase of the infection. Formalin-fixed and parafin-embedded tissues were submitted to Eosin-Hematoxilin, Indirect Immunofluorescence (IFA) and Peroxidase Anti-Peroxidase (PAP) techniques. The Eosin-Hematoxilin showed to be efficient in the analysis of the morphological alterations in the tissues, but did not detect any form of the parasite. Both, IFA and PAP revealed taquizoites of T. gondii, although the last one showed to be more effective because it also allowed an analysis of the morphological tissue alterations.   Seis suínos mestiços Landrace-Large White, com aproximadamente 120 dias de idade, foram inoculados, via endovenosa, com taquizoitos das cepas AS 28, LIV-IV e N de Toxoplasma gondii e analisados quanto aos aspectos clínico, sorológico, parasitológico e histopatológico. Para a avaliação das técnicas de diagnóstico post-mortem, os animais foram sacrificados e necropsiados nas fases aguda (n=3) e crônica (n=3) da infecção. Tecidos formolizados e parafinados foram submetidos às técnicas de Hematoxilina-Eosina (H.E.), Imunofluorescência Indireta (RIFI) e Peroxidase Anti-Peroxidase (PAP). A H.E. revelou-se eficiente na análise das alterações morfológicas dos diferentes tecidos, mas não se mostrou útil para detectar T. gondii. A RIFI e a PAP revelaram o parasito na intimidade dos tecidos, sendo que a última mostrou-se mais eficiente, uma vez que, além de demonstrar parasitas, permitiu a análise das alterações morfológicas dos órgãos estudados.  UEL1995-01-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/431710.5433/1679-0359.1995v16n1p107Semina: Ciências Agrárias; Vol. 16 No. 1 (1995); 107-114Semina: Ciências Agrárias; v. 16 n. 1 (1995); 107-1141679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4317/3574Viotti, Neusa Maria AlvesFreire, Roberta LemosNavarro, Italmar TeodoricoVidotto, Odiloninfo:eu-repo/semantics/openAccess2010-03-22T14:03:46Zoai:ojs.pkp.sfu.ca:article/4317Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2010-03-22T14:03:46Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Evaluation histological, immunofluorescence and immunohistochemistry techniques for the postmortem diagnostic of the swine toxoplasmosis.
Avaliação das técnicas de hematoxilina-eosina, imunofluorescência e peroxidase anti-peroxidase no diagnóstico post-mortem da toxoplasmose suína
title Evaluation histological, immunofluorescence and immunohistochemistry techniques for the postmortem diagnostic of the swine toxoplasmosis.
spellingShingle Evaluation histological, immunofluorescence and immunohistochemistry techniques for the postmortem diagnostic of the swine toxoplasmosis.
Viotti, Neusa Maria Alves
Toxoplasmosis
Histopatology
Immunohistochemistry
Immunofluorescence
Swine
Toxoplasmose
Histopatologia
Imunohistoquímica
Imunofluorescência
Suínos.
title_short Evaluation histological, immunofluorescence and immunohistochemistry techniques for the postmortem diagnostic of the swine toxoplasmosis.
title_full Evaluation histological, immunofluorescence and immunohistochemistry techniques for the postmortem diagnostic of the swine toxoplasmosis.
title_fullStr Evaluation histological, immunofluorescence and immunohistochemistry techniques for the postmortem diagnostic of the swine toxoplasmosis.
title_full_unstemmed Evaluation histological, immunofluorescence and immunohistochemistry techniques for the postmortem diagnostic of the swine toxoplasmosis.
title_sort Evaluation histological, immunofluorescence and immunohistochemistry techniques for the postmortem diagnostic of the swine toxoplasmosis.
author Viotti, Neusa Maria Alves
author_facet Viotti, Neusa Maria Alves
Freire, Roberta Lemos
Navarro, Italmar Teodorico
Vidotto, Odilon
author_role author
author2 Freire, Roberta Lemos
Navarro, Italmar Teodorico
Vidotto, Odilon
author2_role author
author
author
dc.contributor.author.fl_str_mv Viotti, Neusa Maria Alves
Freire, Roberta Lemos
Navarro, Italmar Teodorico
Vidotto, Odilon
dc.subject.por.fl_str_mv Toxoplasmosis
Histopatology
Immunohistochemistry
Immunofluorescence
Swine
Toxoplasmose
Histopatologia
Imunohistoquímica
Imunofluorescência
Suínos.
topic Toxoplasmosis
Histopatology
Immunohistochemistry
Immunofluorescence
Swine
Toxoplasmose
Histopatologia
Imunohistoquímica
Imunofluorescência
Suínos.
description Six 120 days old Landrace/Large-white swines with were inoculated with taquizoites of the AS 28, LIV-IV and N Toxoplasma gondii strains and analysed under clinical, sorological, parasitological and histopathological aspects. In order to evaluate some postmortem diagnostic techniques, three swines were sacrificed in the acute phase and three swines in the chronic phase of the infection. Formalin-fixed and parafin-embedded tissues were submitted to Eosin-Hematoxilin, Indirect Immunofluorescence (IFA) and Peroxidase Anti-Peroxidase (PAP) techniques. The Eosin-Hematoxilin showed to be efficient in the analysis of the morphological alterations in the tissues, but did not detect any form of the parasite. Both, IFA and PAP revealed taquizoites of T. gondii, although the last one showed to be more effective because it also allowed an analysis of the morphological tissue alterations.  
publishDate 1995
dc.date.none.fl_str_mv 1995-01-25
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4317
10.5433/1679-0359.1995v16n1p107
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4317
identifier_str_mv 10.5433/1679-0359.1995v16n1p107
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4317/3574
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 16 No. 1 (1995); 107-114
Semina: Ciências Agrárias; v. 16 n. 1 (1995); 107-114
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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