Oxidative stress and viability of adipose tissue-derived mesenchymal cell cultures from the omentum of rabbits

Detalhes bibliográficos
Autor(a) principal: Pinto Filho, Saulo Tadeu Lemos
Data de Publicação: 2018
Outros Autores: Rosa, Matheus Pippi da, Treichel, Tiago Luis Eilers, Dalmolin, Fabíola, Machado, Alencar Kolinski, Cadoná, Francine Carla, Cruz, Ivana Beatrice Mânica da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/29027
Resumo: Mesenchymal stem cells are a population of somatic cells found in several tissues of an adult organism, including adipose tissue. Reactive oxygen species (ROS) can cause cellular alterations, including mutagenesis and genomic instability and the development of diseases. Thus, it is important to understand ROS-induced damage to cell macromolecules such as DNA, proteins, and lipids. In this study, we investigated oxidative stress rates and viability of adipose tissue-derived mesenchymal stem cells (ADSCs) from the greater omentum of rabbits. Cell cultures were analyzed at different passages (1-5) using the dichlorofluorescein acetate assay for measuring ROS production and cell viability tests. ROS levels were highest at passage 2 and cell viability was highest at passage 4.
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spelling Oxidative stress and viability of adipose tissue-derived mesenchymal cell cultures from the omentum of rabbitsEstresse oxidativo e instabilidade genômica em culturas de células mesenquimais do tecido adiposo do omento de coelhoCell therapyMSCOryctolagus cuniculusROS.Terapia celularCTMOryctolagus cuniculusERO.Mesenchymal stem cells are a population of somatic cells found in several tissues of an adult organism, including adipose tissue. Reactive oxygen species (ROS) can cause cellular alterations, including mutagenesis and genomic instability and the development of diseases. Thus, it is important to understand ROS-induced damage to cell macromolecules such as DNA, proteins, and lipids. In this study, we investigated oxidative stress rates and viability of adipose tissue-derived mesenchymal stem cells (ADSCs) from the greater omentum of rabbits. Cell cultures were analyzed at different passages (1-5) using the dichlorofluorescein acetate assay for measuring ROS production and cell viability tests. ROS levels were highest at passage 2 and cell viability was highest at passage 4.Células-tronco mesenquimais são uma população de células somáticas encontradas em vários tecidos de um organismo adulto, incluindo o tecido adiposo. Espécies reativas de oxigênio (ROS) podem causar alterações celulares, incluindo mutagênese e instabilidade genômica e o desenvolvimento de doenças. Assim, é importante entender o dano induzido pelas EROs às macromoléculas celulares, como DNA, proteínas e lipídios. Neste estudo, investigamos as taxas de estresse oxidativo e viabilidade de células-tronco mesenquimais derivadas do tecido adiposo (ADSCs) a partir do omento maior de coelhos. As culturas celulares foram analisadas em diferentes passagens (1-5) utilizando o ensaio de acetato de diclorofluoresceína para medir a produção de ROS e testes de viabilidade celular. Os níveis de EROs foram mais altos na 2ª passagem e a viabilidade celular foi maior na 4ª passagem.UEL2018-08-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa em Laboratórioapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2902710.5433/1679-0359.2018v39n5p1993Semina: Ciências Agrárias; Vol. 39 No. 5 (2018); 1993-2000Semina: Ciências Agrárias; v. 39 n. 5 (2018); 1993-20001679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/29027/24314Copyright (c) 2018 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessPinto Filho, Saulo Tadeu LemosRosa, Matheus Pippi daTreichel, Tiago Luis EilersDalmolin, FabíolaMachado, Alencar KolinskiCadoná, Francine CarlaCruz, Ivana Beatrice Mânica da2022-10-20T17:52:38Zoai:ojs.pkp.sfu.ca:article/29027Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-20T17:52:38Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Oxidative stress and viability of adipose tissue-derived mesenchymal cell cultures from the omentum of rabbits
Estresse oxidativo e instabilidade genômica em culturas de células mesenquimais do tecido adiposo do omento de coelho
title Oxidative stress and viability of adipose tissue-derived mesenchymal cell cultures from the omentum of rabbits
spellingShingle Oxidative stress and viability of adipose tissue-derived mesenchymal cell cultures from the omentum of rabbits
Pinto Filho, Saulo Tadeu Lemos
Cell therapy
MSC
Oryctolagus cuniculus
ROS.
Terapia celular
CTM
Oryctolagus cuniculus
ERO.
title_short Oxidative stress and viability of adipose tissue-derived mesenchymal cell cultures from the omentum of rabbits
title_full Oxidative stress and viability of adipose tissue-derived mesenchymal cell cultures from the omentum of rabbits
title_fullStr Oxidative stress and viability of adipose tissue-derived mesenchymal cell cultures from the omentum of rabbits
title_full_unstemmed Oxidative stress and viability of adipose tissue-derived mesenchymal cell cultures from the omentum of rabbits
title_sort Oxidative stress and viability of adipose tissue-derived mesenchymal cell cultures from the omentum of rabbits
author Pinto Filho, Saulo Tadeu Lemos
author_facet Pinto Filho, Saulo Tadeu Lemos
Rosa, Matheus Pippi da
Treichel, Tiago Luis Eilers
Dalmolin, Fabíola
Machado, Alencar Kolinski
Cadoná, Francine Carla
Cruz, Ivana Beatrice Mânica da
author_role author
author2 Rosa, Matheus Pippi da
Treichel, Tiago Luis Eilers
Dalmolin, Fabíola
Machado, Alencar Kolinski
Cadoná, Francine Carla
Cruz, Ivana Beatrice Mânica da
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pinto Filho, Saulo Tadeu Lemos
Rosa, Matheus Pippi da
Treichel, Tiago Luis Eilers
Dalmolin, Fabíola
Machado, Alencar Kolinski
Cadoná, Francine Carla
Cruz, Ivana Beatrice Mânica da
dc.subject.por.fl_str_mv Cell therapy
MSC
Oryctolagus cuniculus
ROS.
Terapia celular
CTM
Oryctolagus cuniculus
ERO.
topic Cell therapy
MSC
Oryctolagus cuniculus
ROS.
Terapia celular
CTM
Oryctolagus cuniculus
ERO.
description Mesenchymal stem cells are a population of somatic cells found in several tissues of an adult organism, including adipose tissue. Reactive oxygen species (ROS) can cause cellular alterations, including mutagenesis and genomic instability and the development of diseases. Thus, it is important to understand ROS-induced damage to cell macromolecules such as DNA, proteins, and lipids. In this study, we investigated oxidative stress rates and viability of adipose tissue-derived mesenchymal stem cells (ADSCs) from the greater omentum of rabbits. Cell cultures were analyzed at different passages (1-5) using the dichlorofluorescein acetate assay for measuring ROS production and cell viability tests. ROS levels were highest at passage 2 and cell viability was highest at passage 4.
publishDate 2018
dc.date.none.fl_str_mv 2018-08-20
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Pesquisa em Laboratório
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/29027
10.5433/1679-0359.2018v39n5p1993
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/29027
identifier_str_mv 10.5433/1679-0359.2018v39n5p1993
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/29027/24314
dc.rights.driver.fl_str_mv Copyright (c) 2018 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 39 No. 5 (2018); 1993-2000
Semina: Ciências Agrárias; v. 39 n. 5 (2018); 1993-2000
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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