Serological and molecular detection of Toxoplasma gondii in dogs of urban and rural areas of Cuiaba, Mato Grosso
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/19209 |
Resumo: | This study aimed to determine the prevalence of Toxoplasma gondii infection using serological and molecular analyses in dogs from Cuiabá, a municipality of the Brazilian Cerrado, and the associated factors involved in the transmission cycle. A cross-sectional study was conducted with dogs from the rural and urban areas of Cuiabá (Mato Grosso) from February 2010 to January 2011, and antibodies and the DNA of Toxoplasma gondii were evaluated using indirect immunofluorescence (IFA) and polymerase chain reaction (PCR). In the study, a total of 269 dogs were evaluated using IFA considering positive titer threshold of ? 16 and buffy coat PCR. Of the 269 dogs surveyed, 48.7% had anti-Toxoplasma gondii antibodies according to IFA, and 15.6% had the parasite’s DNA. The seroprevalence was 62.4% in the rural districts and 40.4% in the urban areas, and the difference between these areas was significant (p = 0.0007). The most common levels of antibody titers were 51 in 256 (39%) dogs, followed by 1024 in 37 (28.2%) dogs. The infectious agent was associated with breed, age, access to the street and the environment in which the animal lived (p <0.05). The serological and molecular results showed that T. gondii infection is active in the canine population in the rural and urban areas of Cuiabá, with a higher risk in dogs residing in rural areas. |
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Semina. Ciências Agrárias (Online) |
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Serological and molecular detection of Toxoplasma gondii in dogs of urban and rural areas of Cuiaba, Mato GrossoDetecção sorológica e molecular de Toxoplasma gondii em cães de áreas urbanas e rurais de Cuiabá, Mato GrossoDogAssociated factorsPCRSerologyToxoplasmosis.CãoFatores de riscoPCRSorologiaToxoplasmose.This study aimed to determine the prevalence of Toxoplasma gondii infection using serological and molecular analyses in dogs from Cuiabá, a municipality of the Brazilian Cerrado, and the associated factors involved in the transmission cycle. A cross-sectional study was conducted with dogs from the rural and urban areas of Cuiabá (Mato Grosso) from February 2010 to January 2011, and antibodies and the DNA of Toxoplasma gondii were evaluated using indirect immunofluorescence (IFA) and polymerase chain reaction (PCR). In the study, a total of 269 dogs were evaluated using IFA considering positive titer threshold of ? 16 and buffy coat PCR. Of the 269 dogs surveyed, 48.7% had anti-Toxoplasma gondii antibodies according to IFA, and 15.6% had the parasite’s DNA. The seroprevalence was 62.4% in the rural districts and 40.4% in the urban areas, and the difference between these areas was significant (p = 0.0007). The most common levels of antibody titers were 51 in 256 (39%) dogs, followed by 1024 in 37 (28.2%) dogs. The infectious agent was associated with breed, age, access to the street and the environment in which the animal lived (p <0.05). The serological and molecular results showed that T. gondii infection is active in the canine population in the rural and urban areas of Cuiabá, with a higher risk in dogs residing in rural areas.Este artigo teve por objetivo determinar a soroprevalência da infecção por Toxoplasma gondii através de análise sorológica e molecular em cães de Cuiabá, município do cerrado brasileiro, associando com os fatores de risco envolvidos na cadeia de transmissão. Estudo transversal foi conduzido com cães de áreas rurais e urbanas de Cuiabá (Mato Grosso), entre fevereiro de 2010 a janeiro de 2011, através da pesquisa de anticorpos por imunofluorescência indireta e DNA de Toxoplasma gondii. Foram avaliados no estudo um total de 269 cães por IFI, considerando reagente cães com título ? 16 e por PCR de capa leucocitária. Dos 269 cães pesquisados, 48,7% apresentaram anticorpos anti-Toxoplasma gondii pela IFI e 15,6% DNA do parasito. Os bairros localizados em ambiente rural apresentaram uma soroprevalência de 62,4% e os urbanos de 40,4%, dados estes estatisticamente significativos (p= 0,0007). Os títulos mais frequentemente observados foram de 256 em 51 (39%) cães, seguido por 1024 em 37 (28,2%) cães avaliados. A infecção pelo agente esteve associada a raça, idade, acesso à rua e ao ambiente em que o animal vivia (p<0,05). Os resultados sorológicos e moleculares permitiram inferir que a infecção por T. gondii encontra-se ativa na população canina nas regiões rurais e urbanas do município de Cuiabá, com risco maior em cães residentes em áreas rurais. UEL2015-12-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1920910.5433/1679-0359.2015v36n6p3705Semina: Ciências Agrárias; Vol. 36 No. 6 (2015); 3705-3712Semina: Ciências Agrárias; v. 36 n. 6 (2015); 3705-37121679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/19209/17453http://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessBoa Sorte, Eveline da CruzAlmeida, Arleana Bom Parto Ferreira deCruz, Felipe Augusto Constantino Seabra daGasparetto, Naiani DomingosGodoy, Isabela deDutra, ValériaAmendoeira, Maria Regina ReisSousa, Valéria Régia Franco2022-12-02T16:32:29Zoai:ojs.pkp.sfu.ca:article/19209Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-12-02T16:32:29Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Serological and molecular detection of Toxoplasma gondii in dogs of urban and rural areas of Cuiaba, Mato Grosso Detecção sorológica e molecular de Toxoplasma gondii em cães de áreas urbanas e rurais de Cuiabá, Mato Grosso |
title |
Serological and molecular detection of Toxoplasma gondii in dogs of urban and rural areas of Cuiaba, Mato Grosso |
spellingShingle |
Serological and molecular detection of Toxoplasma gondii in dogs of urban and rural areas of Cuiaba, Mato Grosso Boa Sorte, Eveline da Cruz Dog Associated factors PCR Serology Toxoplasmosis. Cão Fatores de risco PCR Sorologia Toxoplasmose. |
title_short |
Serological and molecular detection of Toxoplasma gondii in dogs of urban and rural areas of Cuiaba, Mato Grosso |
title_full |
Serological and molecular detection of Toxoplasma gondii in dogs of urban and rural areas of Cuiaba, Mato Grosso |
title_fullStr |
Serological and molecular detection of Toxoplasma gondii in dogs of urban and rural areas of Cuiaba, Mato Grosso |
title_full_unstemmed |
Serological and molecular detection of Toxoplasma gondii in dogs of urban and rural areas of Cuiaba, Mato Grosso |
title_sort |
Serological and molecular detection of Toxoplasma gondii in dogs of urban and rural areas of Cuiaba, Mato Grosso |
author |
Boa Sorte, Eveline da Cruz |
author_facet |
Boa Sorte, Eveline da Cruz Almeida, Arleana Bom Parto Ferreira de Cruz, Felipe Augusto Constantino Seabra da Gasparetto, Naiani Domingos Godoy, Isabela de Dutra, Valéria Amendoeira, Maria Regina Reis Sousa, Valéria Régia Franco |
author_role |
author |
author2 |
Almeida, Arleana Bom Parto Ferreira de Cruz, Felipe Augusto Constantino Seabra da Gasparetto, Naiani Domingos Godoy, Isabela de Dutra, Valéria Amendoeira, Maria Regina Reis Sousa, Valéria Régia Franco |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Boa Sorte, Eveline da Cruz Almeida, Arleana Bom Parto Ferreira de Cruz, Felipe Augusto Constantino Seabra da Gasparetto, Naiani Domingos Godoy, Isabela de Dutra, Valéria Amendoeira, Maria Regina Reis Sousa, Valéria Régia Franco |
dc.subject.por.fl_str_mv |
Dog Associated factors PCR Serology Toxoplasmosis. Cão Fatores de risco PCR Sorologia Toxoplasmose. |
topic |
Dog Associated factors PCR Serology Toxoplasmosis. Cão Fatores de risco PCR Sorologia Toxoplasmose. |
description |
This study aimed to determine the prevalence of Toxoplasma gondii infection using serological and molecular analyses in dogs from Cuiabá, a municipality of the Brazilian Cerrado, and the associated factors involved in the transmission cycle. A cross-sectional study was conducted with dogs from the rural and urban areas of Cuiabá (Mato Grosso) from February 2010 to January 2011, and antibodies and the DNA of Toxoplasma gondii were evaluated using indirect immunofluorescence (IFA) and polymerase chain reaction (PCR). In the study, a total of 269 dogs were evaluated using IFA considering positive titer threshold of ? 16 and buffy coat PCR. Of the 269 dogs surveyed, 48.7% had anti-Toxoplasma gondii antibodies according to IFA, and 15.6% had the parasite’s DNA. The seroprevalence was 62.4% in the rural districts and 40.4% in the urban areas, and the difference between these areas was significant (p = 0.0007). The most common levels of antibody titers were 51 in 256 (39%) dogs, followed by 1024 in 37 (28.2%) dogs. The infectious agent was associated with breed, age, access to the street and the environment in which the animal lived (p <0.05). The serological and molecular results showed that T. gondii infection is active in the canine population in the rural and urban areas of Cuiabá, with a higher risk in dogs residing in rural areas. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-09 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/19209 10.5433/1679-0359.2015v36n6p3705 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/19209 |
identifier_str_mv |
10.5433/1679-0359.2015v36n6p3705 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/19209/17453 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 36 No. 6 (2015); 3705-3712 Semina: Ciências Agrárias; v. 36 n. 6 (2015); 3705-3712 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306072691834880 |