Serological and molecular detection of Toxoplasma gondii in dogs of urban and rural areas of Cuiaba, Mato Grosso

Detalhes bibliográficos
Autor(a) principal: Boa Sorte, Eveline da Cruz
Data de Publicação: 2015
Outros Autores: Almeida, Arleana Bom Parto Ferreira de, Cruz, Felipe Augusto Constantino Seabra da, Gasparetto, Naiani Domingos, Godoy, Isabela de, Dutra, Valéria, Amendoeira, Maria Regina Reis, Sousa, Valéria Régia Franco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/19209
Resumo: This study aimed to determine the prevalence of Toxoplasma gondii infection using serological and molecular analyses in dogs from Cuiabá, a municipality of the Brazilian Cerrado, and the associated factors involved in the transmission cycle. A cross-sectional study was conducted with dogs from the rural and urban areas of Cuiabá (Mato Grosso) from February 2010 to January 2011, and antibodies and the DNA of Toxoplasma gondii were evaluated using indirect immunofluorescence (IFA) and polymerase chain reaction (PCR). In the study, a total of 269 dogs were evaluated using IFA considering positive titer threshold of ? 16 and buffy coat PCR. Of the 269 dogs surveyed, 48.7% had anti-Toxoplasma gondii antibodies according to IFA, and 15.6% had the parasite’s DNA. The seroprevalence was 62.4% in the rural districts and 40.4% in the urban areas, and the difference between these areas was significant (p = 0.0007). The most common levels of antibody titers were 51 in 256 (39%) dogs, followed by 1024 in 37 (28.2%) dogs. The infectious agent was associated with breed, age, access to the street and the environment in which the animal lived (p <0.05). The serological and molecular results showed that T. gondii infection is active in the canine population in the rural and urban areas of Cuiabá, with a higher risk in dogs residing in rural areas.
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spelling Serological and molecular detection of Toxoplasma gondii in dogs of urban and rural areas of Cuiaba, Mato GrossoDetecção sorológica e molecular de Toxoplasma gondii em cães de áreas urbanas e rurais de Cuiabá, Mato GrossoDogAssociated factorsPCRSerologyToxoplasmosis.CãoFatores de riscoPCRSorologiaToxoplasmose.This study aimed to determine the prevalence of Toxoplasma gondii infection using serological and molecular analyses in dogs from Cuiabá, a municipality of the Brazilian Cerrado, and the associated factors involved in the transmission cycle. A cross-sectional study was conducted with dogs from the rural and urban areas of Cuiabá (Mato Grosso) from February 2010 to January 2011, and antibodies and the DNA of Toxoplasma gondii were evaluated using indirect immunofluorescence (IFA) and polymerase chain reaction (PCR). In the study, a total of 269 dogs were evaluated using IFA considering positive titer threshold of ? 16 and buffy coat PCR. Of the 269 dogs surveyed, 48.7% had anti-Toxoplasma gondii antibodies according to IFA, and 15.6% had the parasite’s DNA. The seroprevalence was 62.4% in the rural districts and 40.4% in the urban areas, and the difference between these areas was significant (p = 0.0007). The most common levels of antibody titers were 51 in 256 (39%) dogs, followed by 1024 in 37 (28.2%) dogs. The infectious agent was associated with breed, age, access to the street and the environment in which the animal lived (p <0.05). The serological and molecular results showed that T. gondii infection is active in the canine population in the rural and urban areas of Cuiabá, with a higher risk in dogs residing in rural areas.Este artigo teve por objetivo determinar a soroprevalência da infecção por Toxoplasma gondii através de análise sorológica e molecular em cães de Cuiabá, município do cerrado brasileiro, associando com os fatores de risco envolvidos na cadeia de transmissão. Estudo transversal foi conduzido com cães de áreas rurais e urbanas de Cuiabá (Mato Grosso), entre fevereiro de 2010 a janeiro de 2011, através da pesquisa de anticorpos por imunofluorescência indireta e DNA de Toxoplasma gondii. Foram avaliados no estudo um total de 269 cães por IFI, considerando reagente cães com título ? 16 e por PCR de capa leucocitária. Dos 269 cães pesquisados, 48,7% apresentaram anticorpos anti-Toxoplasma gondii pela IFI e 15,6% DNA do parasito. Os bairros localizados em ambiente rural apresentaram uma soroprevalência de 62,4% e os urbanos de 40,4%, dados estes estatisticamente significativos (p= 0,0007). Os títulos mais frequentemente observados foram de 256 em 51 (39%) cães, seguido por 1024 em 37 (28,2%) cães avaliados. A infecção pelo agente esteve associada a raça, idade, acesso à rua e ao ambiente em que o animal vivia (p<0,05). Os resultados sorológicos e moleculares permitiram inferir que a infecção por T. gondii encontra-se ativa na população canina nas regiões rurais e urbanas do município de Cuiabá, com risco maior em cães residentes em áreas rurais. UEL2015-12-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1920910.5433/1679-0359.2015v36n6p3705Semina: Ciências Agrárias; Vol. 36 No. 6 (2015); 3705-3712Semina: Ciências Agrárias; v. 36 n. 6 (2015); 3705-37121679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/19209/17453http://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessBoa Sorte, Eveline da CruzAlmeida, Arleana Bom Parto Ferreira deCruz, Felipe Augusto Constantino Seabra daGasparetto, Naiani DomingosGodoy, Isabela deDutra, ValériaAmendoeira, Maria Regina ReisSousa, Valéria Régia Franco2022-12-02T16:32:29Zoai:ojs.pkp.sfu.ca:article/19209Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-12-02T16:32:29Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Serological and molecular detection of Toxoplasma gondii in dogs of urban and rural areas of Cuiaba, Mato Grosso
Detecção sorológica e molecular de Toxoplasma gondii em cães de áreas urbanas e rurais de Cuiabá, Mato Grosso
title Serological and molecular detection of Toxoplasma gondii in dogs of urban and rural areas of Cuiaba, Mato Grosso
spellingShingle Serological and molecular detection of Toxoplasma gondii in dogs of urban and rural areas of Cuiaba, Mato Grosso
Boa Sorte, Eveline da Cruz
Dog
Associated factors
PCR
Serology
Toxoplasmosis.
Cão
Fatores de risco
PCR
Sorologia
Toxoplasmose.
title_short Serological and molecular detection of Toxoplasma gondii in dogs of urban and rural areas of Cuiaba, Mato Grosso
title_full Serological and molecular detection of Toxoplasma gondii in dogs of urban and rural areas of Cuiaba, Mato Grosso
title_fullStr Serological and molecular detection of Toxoplasma gondii in dogs of urban and rural areas of Cuiaba, Mato Grosso
title_full_unstemmed Serological and molecular detection of Toxoplasma gondii in dogs of urban and rural areas of Cuiaba, Mato Grosso
title_sort Serological and molecular detection of Toxoplasma gondii in dogs of urban and rural areas of Cuiaba, Mato Grosso
author Boa Sorte, Eveline da Cruz
author_facet Boa Sorte, Eveline da Cruz
Almeida, Arleana Bom Parto Ferreira de
Cruz, Felipe Augusto Constantino Seabra da
Gasparetto, Naiani Domingos
Godoy, Isabela de
Dutra, Valéria
Amendoeira, Maria Regina Reis
Sousa, Valéria Régia Franco
author_role author
author2 Almeida, Arleana Bom Parto Ferreira de
Cruz, Felipe Augusto Constantino Seabra da
Gasparetto, Naiani Domingos
Godoy, Isabela de
Dutra, Valéria
Amendoeira, Maria Regina Reis
Sousa, Valéria Régia Franco
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Boa Sorte, Eveline da Cruz
Almeida, Arleana Bom Parto Ferreira de
Cruz, Felipe Augusto Constantino Seabra da
Gasparetto, Naiani Domingos
Godoy, Isabela de
Dutra, Valéria
Amendoeira, Maria Regina Reis
Sousa, Valéria Régia Franco
dc.subject.por.fl_str_mv Dog
Associated factors
PCR
Serology
Toxoplasmosis.
Cão
Fatores de risco
PCR
Sorologia
Toxoplasmose.
topic Dog
Associated factors
PCR
Serology
Toxoplasmosis.
Cão
Fatores de risco
PCR
Sorologia
Toxoplasmose.
description This study aimed to determine the prevalence of Toxoplasma gondii infection using serological and molecular analyses in dogs from Cuiabá, a municipality of the Brazilian Cerrado, and the associated factors involved in the transmission cycle. A cross-sectional study was conducted with dogs from the rural and urban areas of Cuiabá (Mato Grosso) from February 2010 to January 2011, and antibodies and the DNA of Toxoplasma gondii were evaluated using indirect immunofluorescence (IFA) and polymerase chain reaction (PCR). In the study, a total of 269 dogs were evaluated using IFA considering positive titer threshold of ? 16 and buffy coat PCR. Of the 269 dogs surveyed, 48.7% had anti-Toxoplasma gondii antibodies according to IFA, and 15.6% had the parasite’s DNA. The seroprevalence was 62.4% in the rural districts and 40.4% in the urban areas, and the difference between these areas was significant (p = 0.0007). The most common levels of antibody titers were 51 in 256 (39%) dogs, followed by 1024 in 37 (28.2%) dogs. The infectious agent was associated with breed, age, access to the street and the environment in which the animal lived (p <0.05). The serological and molecular results showed that T. gondii infection is active in the canine population in the rural and urban areas of Cuiabá, with a higher risk in dogs residing in rural areas.
publishDate 2015
dc.date.none.fl_str_mv 2015-12-09
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/19209
10.5433/1679-0359.2015v36n6p3705
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/19209
identifier_str_mv 10.5433/1679-0359.2015v36n6p3705
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/19209/17453
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 36 No. 6 (2015); 3705-3712
Semina: Ciências Agrárias; v. 36 n. 6 (2015); 3705-3712
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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