First molecular detection of Eimeria spp. in eared doves (Zenaida auriculata) from Brazil
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36964 |
Resumo: | The aim of the present study was to detect Eimeria spp. in eared doves (Zenaida auriculata) from Brazil. Two hundred and fourteen birds were trap-capture in different regions of Londrina city, Paraná state, Southern Brazil. Fecal samples were collected, and DNA extraction was performed. A nested PCR based on the subunit I of the cytochrome c oxidase gene of the Eimeria mitochondrial genome was used to detect the DNA presence of this coccidian in eared dove feces. From 214 birds, 171 (79.9%) were positive for Eimeria spp. There was significantly difference of positivity between the site of capture. When analyzing the genders, the numbers of positive males were 84/103 (81.5%) and females 87/111 (78.4%). To the best of the authors’ knowledge, this is the first study with molecular prevalence of Eimeria sp. in Z. auriculata. Further studies should be done to identify the species of Eimeria that infect eared doves Z. auriculata. |
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Semina. Ciências Agrárias (Online) |
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First molecular detection of Eimeria spp. in eared doves (Zenaida auriculata) from BrazilPrimeira detecção molecular de Eimeria spp. em pombos (Zenaida auriculata) do BrasilEimeriidaeCoccidiosisPCRColumbiformesCytochrome c oxidase I gene.EimeriidaeCoccidiosePCRColumbiformesCitocromo c oxidase I gene.The aim of the present study was to detect Eimeria spp. in eared doves (Zenaida auriculata) from Brazil. Two hundred and fourteen birds were trap-capture in different regions of Londrina city, Paraná state, Southern Brazil. Fecal samples were collected, and DNA extraction was performed. A nested PCR based on the subunit I of the cytochrome c oxidase gene of the Eimeria mitochondrial genome was used to detect the DNA presence of this coccidian in eared dove feces. From 214 birds, 171 (79.9%) were positive for Eimeria spp. There was significantly difference of positivity between the site of capture. When analyzing the genders, the numbers of positive males were 84/103 (81.5%) and females 87/111 (78.4%). To the best of the authors’ knowledge, this is the first study with molecular prevalence of Eimeria sp. in Z. auriculata. Further studies should be done to identify the species of Eimeria that infect eared doves Z. auriculata.O objetivo do presente estudo foi detectar Eimeria spp. em pombos (Zenaida auriculata) do Brasil. Duzentos e quatorze pombos foram capturados em diferentes regiões da cidade de Londrina, estado do Paraná, Sul do Brasil. Amostras fecais foram coletadas e realizada a extração de DNA. Uma nested-PCR baseada na subunidade I do gene oxidase do citocromo c oxidase do genoma mitocondrial de Eimeria spp. foi utilizada para detectar a presença de DNA deste coccídeo nas amostras fecais dos pombos. Das 214 aves, 171 (79,9%) foram positivas para Eimeria spp. Houve uma diferença significativa de positividade entre o local de captura. Quando analisado o gênero, o número de machos positivos foi 84/103 (81,5%) e fêmeas 87/111 (78,4%). Para o conhecimento, este é o primeiro estudo com prevalência molecular de Eimeria spp. em Z. auriculata. Novos estudos devem ser conduzidos para identificar as espécies de Eimeria que infectam pombos Z. auriculata.UEL2020-05-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3696410.5433/1679-0359.2020v41n4p1259Semina: Ciências Agrárias; Vol. 41 No. 4 (2020); 1259-1266Semina: Ciências Agrárias; v. 41 n. 4 (2020); 1259-12661679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36964/27346Copyright (c) 2020 Semina: Ciências Agráriasinfo:eu-repo/semantics/openAccessTaroda, AlessandraBarros, Luiz Daniel deSeixas, Mércia deCardim, Sérgio TosiSasse, João PedroMinutti, Ana FláviaVidotto, OdilonGarcia, João Luis2020-05-13T15:46:00Zoai:ojs.pkp.sfu.ca:article/36964Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2020-05-13T15:46Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
First molecular detection of Eimeria spp. in eared doves (Zenaida auriculata) from Brazil Primeira detecção molecular de Eimeria spp. em pombos (Zenaida auriculata) do Brasil |
title |
First molecular detection of Eimeria spp. in eared doves (Zenaida auriculata) from Brazil |
spellingShingle |
First molecular detection of Eimeria spp. in eared doves (Zenaida auriculata) from Brazil Taroda, Alessandra Eimeriidae Coccidiosis PCR Columbiformes Cytochrome c oxidase I gene. Eimeriidae Coccidiose PCR Columbiformes Citocromo c oxidase I gene. |
title_short |
First molecular detection of Eimeria spp. in eared doves (Zenaida auriculata) from Brazil |
title_full |
First molecular detection of Eimeria spp. in eared doves (Zenaida auriculata) from Brazil |
title_fullStr |
First molecular detection of Eimeria spp. in eared doves (Zenaida auriculata) from Brazil |
title_full_unstemmed |
First molecular detection of Eimeria spp. in eared doves (Zenaida auriculata) from Brazil |
title_sort |
First molecular detection of Eimeria spp. in eared doves (Zenaida auriculata) from Brazil |
author |
Taroda, Alessandra |
author_facet |
Taroda, Alessandra Barros, Luiz Daniel de Seixas, Mércia de Cardim, Sérgio Tosi Sasse, João Pedro Minutti, Ana Flávia Vidotto, Odilon Garcia, João Luis |
author_role |
author |
author2 |
Barros, Luiz Daniel de Seixas, Mércia de Cardim, Sérgio Tosi Sasse, João Pedro Minutti, Ana Flávia Vidotto, Odilon Garcia, João Luis |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Taroda, Alessandra Barros, Luiz Daniel de Seixas, Mércia de Cardim, Sérgio Tosi Sasse, João Pedro Minutti, Ana Flávia Vidotto, Odilon Garcia, João Luis |
dc.subject.por.fl_str_mv |
Eimeriidae Coccidiosis PCR Columbiformes Cytochrome c oxidase I gene. Eimeriidae Coccidiose PCR Columbiformes Citocromo c oxidase I gene. |
topic |
Eimeriidae Coccidiosis PCR Columbiformes Cytochrome c oxidase I gene. Eimeriidae Coccidiose PCR Columbiformes Citocromo c oxidase I gene. |
description |
The aim of the present study was to detect Eimeria spp. in eared doves (Zenaida auriculata) from Brazil. Two hundred and fourteen birds were trap-capture in different regions of Londrina city, Paraná state, Southern Brazil. Fecal samples were collected, and DNA extraction was performed. A nested PCR based on the subunit I of the cytochrome c oxidase gene of the Eimeria mitochondrial genome was used to detect the DNA presence of this coccidian in eared dove feces. From 214 birds, 171 (79.9%) were positive for Eimeria spp. There was significantly difference of positivity between the site of capture. When analyzing the genders, the numbers of positive males were 84/103 (81.5%) and females 87/111 (78.4%). To the best of the authors’ knowledge, this is the first study with molecular prevalence of Eimeria sp. in Z. auriculata. Further studies should be done to identify the species of Eimeria that infect eared doves Z. auriculata. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-05-13 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36964 10.5433/1679-0359.2020v41n4p1259 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36964 |
identifier_str_mv |
10.5433/1679-0359.2020v41n4p1259 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36964/27346 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Semina: Ciências Agrárias info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Semina: Ciências Agrárias |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 41 No. 4 (2020); 1259-1266 Semina: Ciências Agrárias; v. 41 n. 4 (2020); 1259-1266 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306081703297024 |