Humoral immune response in experimental ddY mice Paracoccidioidomycosis

Detalhes bibliográficos
Autor(a) principal: Tatibana, Berenice Tomoko
Data de Publicação: 2007
Outros Autores: Sano, Ayako, Uno, Jun, Mikami, Yuzuru, Miyaji, Makoto, Nishimura, Kazuko, Itano, Eiko Nakagawa
Tipo de documento: Artigo
Idioma: por
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3441
Resumo: Paracoccidioidomycosis (PCM) is a systemic mycosis, restrict to Latin America, with higher incidence in Brazil. ddY mice have been used as experimental PCM model, although there is no data regarding immune response. The aim of the present study was evaluated specific humoral response against the main specific antigen of the fungal Paracoccidioides brasiliensis, the gp43, in ddY mice infected with virulent Pb 18. Antigenemia analysis and histophatological exam in several organs were performed in different time postinfection The results showed increased levels of anti-gp43 IgG on days 14, 17, 21, 24, 28 and 56 post-infection and increased levels of soluble gp-43 on day 28 post-infection. The fungal cells were detected in all organs analyzed (brain, heart, lung, liver, spleen and kidney) in all investigated periods. The granulomatous lesions became predominant 14 days after infection. The results evidence that ddY mice produce humoral immune response to main P. brasiliensis antigen, with high levels until 56 days after infection. Further studies are needed to show that reduction of soluble gp43 in chronic phase correlates with infection control.
id UEL-11_28b2ccd9b6ad1a0451c66e390760dd9c
oai_identifier_str oai:ojs.pkp.sfu.ca:article/3441
network_acronym_str UEL-11
network_name_str Semina. Ciências Agrárias (Online)
repository_id_str
spelling Humoral immune response in experimental ddY mice ParacoccidioidomycosisResposta imune humoral na paracoccidioidomicose experimental em camundongos ddYParacoccidioides brasiliensisexperimental paracoccidioidomycosisDDYimmune responseParacoccidioides brasiliensisparacoccidioidomicose experimentalDDYresposta imuneParacoccidioidomycosis (PCM) is a systemic mycosis, restrict to Latin America, with higher incidence in Brazil. ddY mice have been used as experimental PCM model, although there is no data regarding immune response. The aim of the present study was evaluated specific humoral response against the main specific antigen of the fungal Paracoccidioides brasiliensis, the gp43, in ddY mice infected with virulent Pb 18. Antigenemia analysis and histophatological exam in several organs were performed in different time postinfection The results showed increased levels of anti-gp43 IgG on days 14, 17, 21, 24, 28 and 56 post-infection and increased levels of soluble gp-43 on day 28 post-infection. The fungal cells were detected in all organs analyzed (brain, heart, lung, liver, spleen and kidney) in all investigated periods. The granulomatous lesions became predominant 14 days after infection. The results evidence that ddY mice produce humoral immune response to main P. brasiliensis antigen, with high levels until 56 days after infection. Further studies are needed to show that reduction of soluble gp43 in chronic phase correlates with infection control. A paracoccidioidomicose (PCM) é uma micose sistêmica, restrita à América Latina, com maior incidência no Brasil. O camundongo ddY tem sido empregado como modelo murino de PCM e, no entanto, não há informações a respeito da resposta imune desse animal frente à infecção. O presente estudo tem como objetivo avaliar a resposta imune humoral específica para o principal antígeno, gp43, do fungo Paracoccidioides brasiliensis, em camundongos ddY infectados com a cepa virulenta Pb 18. Foram realizadas análises da antigenemia e histopatológico em vários órgãos e em diferentes tempos pós-infecção. Os resultados obtidos demonstraram aumento nos níveis de IgG anti-gp43 nos dias 14, 17, 21, 24, 28 e 56 pós-infecção e aumento no nível de gp43 solúvel aos 28 dias pós-infecção. As células fúngicas foram detectadas em todos os órgãos analisados (cérebro, coração, pulmão, fígado, baço e rim) e em todos os períodos. As lesões granulomatosas tornaramse predominantes 14 dias pós-infecção. Os resultados evidenciaram que o camundongo ddY produz resposta imune humoral frente ao principal antígeno de P. brasiliensis, apresentando-se elevado até 56 dias pósinfecção. A redução do nível de gp43 solúvel na fase crônica, supostamente devido ao início do controle da infecção, requer estudos complementares adicionais. UEL2007-10-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/344110.5433/1679-0359.2007v28n2p287Semina: Ciências Agrárias; Vol. 28 No. 2 (2007); 287-294Semina: Ciências Agrárias; v. 28 n. 2 (2007); 287-2941679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3441/2797Tatibana, Berenice TomokoSano, AyakoUno, JunMikami, YuzuruMiyaji, MakotoNishimura, KazukoItano, Eiko Nakagawainfo:eu-repo/semantics/openAccess2009-10-08T18:50:47Zoai:ojs.pkp.sfu.ca:article/3441Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2009-10-08T18:50:47Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Humoral immune response in experimental ddY mice Paracoccidioidomycosis
Resposta imune humoral na paracoccidioidomicose experimental em camundongos ddY
title Humoral immune response in experimental ddY mice Paracoccidioidomycosis
spellingShingle Humoral immune response in experimental ddY mice Paracoccidioidomycosis
Tatibana, Berenice Tomoko
Paracoccidioides brasiliensis
experimental paracoccidioidomycosis
DDY
immune response
Paracoccidioides brasiliensis
paracoccidioidomicose experimental
DDY
resposta imune
title_short Humoral immune response in experimental ddY mice Paracoccidioidomycosis
title_full Humoral immune response in experimental ddY mice Paracoccidioidomycosis
title_fullStr Humoral immune response in experimental ddY mice Paracoccidioidomycosis
title_full_unstemmed Humoral immune response in experimental ddY mice Paracoccidioidomycosis
title_sort Humoral immune response in experimental ddY mice Paracoccidioidomycosis
author Tatibana, Berenice Tomoko
author_facet Tatibana, Berenice Tomoko
Sano, Ayako
Uno, Jun
Mikami, Yuzuru
Miyaji, Makoto
Nishimura, Kazuko
Itano, Eiko Nakagawa
author_role author
author2 Sano, Ayako
Uno, Jun
Mikami, Yuzuru
Miyaji, Makoto
Nishimura, Kazuko
Itano, Eiko Nakagawa
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Tatibana, Berenice Tomoko
Sano, Ayako
Uno, Jun
Mikami, Yuzuru
Miyaji, Makoto
Nishimura, Kazuko
Itano, Eiko Nakagawa
dc.subject.por.fl_str_mv Paracoccidioides brasiliensis
experimental paracoccidioidomycosis
DDY
immune response
Paracoccidioides brasiliensis
paracoccidioidomicose experimental
DDY
resposta imune
topic Paracoccidioides brasiliensis
experimental paracoccidioidomycosis
DDY
immune response
Paracoccidioides brasiliensis
paracoccidioidomicose experimental
DDY
resposta imune
description Paracoccidioidomycosis (PCM) is a systemic mycosis, restrict to Latin America, with higher incidence in Brazil. ddY mice have been used as experimental PCM model, although there is no data regarding immune response. The aim of the present study was evaluated specific humoral response against the main specific antigen of the fungal Paracoccidioides brasiliensis, the gp43, in ddY mice infected with virulent Pb 18. Antigenemia analysis and histophatological exam in several organs were performed in different time postinfection The results showed increased levels of anti-gp43 IgG on days 14, 17, 21, 24, 28 and 56 post-infection and increased levels of soluble gp-43 on day 28 post-infection. The fungal cells were detected in all organs analyzed (brain, heart, lung, liver, spleen and kidney) in all investigated periods. The granulomatous lesions became predominant 14 days after infection. The results evidence that ddY mice produce humoral immune response to main P. brasiliensis antigen, with high levels until 56 days after infection. Further studies are needed to show that reduction of soluble gp43 in chronic phase correlates with infection control.
publishDate 2007
dc.date.none.fl_str_mv 2007-10-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3441
10.5433/1679-0359.2007v28n2p287
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3441
identifier_str_mv 10.5433/1679-0359.2007v28n2p287
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3441/2797
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 28 No. 2 (2007); 287-294
Semina: Ciências Agrárias; v. 28 n. 2 (2007); 287-294
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
_version_ 1799306055800324096

Registros relacionados