Isoquinolone alkaloids mitigate microscopic digestive tract lesions induced by sub-acute ruminal acidosis (SARA) in feedlot cattle
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36073 |
Resumo: | Feedlot cattle is submitted to a diet rich in energy and reduced in fibres that induces the sub-acute ruminal acidosis (SARA) with its lesions and clinical signs. Recent studies have demonstrated some amelioration of this condition by the use of isoquinolone alkaloids found in Macleaya cordata (Papaveraceae) such as Sanguinarine and Chelerythrine. These compounds have demonstrated antimicrobial, anti-in?ammatory and immune-modulatory effects in both humans and animals The aim on this study, using histopathology and a score system, was to evaluate the differences between a non-treated and a treated group feed with these alkaloids, present in trade preparation Sangrovit-RS® as a source of sanguinarine (SG), chelerythrine (CH) and protropine (PA) standardized to 0.15% w/w SG, using feedlot cattle under a high-grain diet as an inflammatory model for gastrointestinal system. The samples of forestomachs were evaluated and graded using scores ranging from zero (0) to three (3) obtained at light-microscopic fields of 400X. Parameters such as inflammation, hydropic degeneration, hyperkeratosis, and vesicle formation were accessed in the different layers of the tissues, considering the severity and dispersion of the microscopic lesions. The soft tissues such as the abomasum, small intestine, cecum and colon had their total amount of inflammatory cells counted at light-microscopic fields of 200X. The rumen of the SG-CH-PRO-treated group showed a significant reduction in the epithelial hydropic degeneration scores (p ? 0.001) and lamina propria inflammation (p ? 0.001).The reticulum had a similar reduction in scores of epithelial (p ? 0.002) and stratum corneum hydropic degeneration (p ? 0.001), hyperkeratosis (p ? 0.002) and inflammation in lamina propria (p ? 0.001) and epithelium (p ? 0.002). The omasum had no significant differences. All non-keratinized tissues, except for ileum, had a significant decrease (p ? 0.001) in the total counting of inflammatory cells. In this trial, the feedlot cattle feed with high grain diet and treated with isoquinolone alkaloids expressed lesions that indicate ameliorations and worsening’s. Ameliorating effects of the alkaloids were better demonstrated in tissues with reduced or no corneal layer in the mucosa and in the absence of a lipopolysaccharides rich acidic environment reinforcing the notion of the topic action, the dependence of the media pH and the time of exposure modulating the pharmacological mechanisms of these alkaloids. The observed cytolytic (oncolysis) effect in epithelial forestomachs cells under low pH values, worsening the osmotic status, should be considered before clinical applications. |
id |
UEL-11_2d9c7843429bfe601d29703955b99a89 |
---|---|
oai_identifier_str |
oai:ojs.pkp.sfu.ca:article/36073 |
network_acronym_str |
UEL-11 |
network_name_str |
Semina. Ciências Agrárias (Online) |
repository_id_str |
|
spelling |
Isoquinolone alkaloids mitigate microscopic digestive tract lesions induced by sub-acute ruminal acidosis (SARA) in feedlot cattleAlcaloides isoquinolínicos atenuam lesões microscópicas digestivas induzidas por acidose ruminal subaguda em bovinos confinadosChelerythrine Na K - ATPase pumpRuminitisSanguinarine.Bomba Na K - ATPaseQueleritrinaRumenitisSanguinarina.Feedlot cattle is submitted to a diet rich in energy and reduced in fibres that induces the sub-acute ruminal acidosis (SARA) with its lesions and clinical signs. Recent studies have demonstrated some amelioration of this condition by the use of isoquinolone alkaloids found in Macleaya cordata (Papaveraceae) such as Sanguinarine and Chelerythrine. These compounds have demonstrated antimicrobial, anti-in?ammatory and immune-modulatory effects in both humans and animals The aim on this study, using histopathology and a score system, was to evaluate the differences between a non-treated and a treated group feed with these alkaloids, present in trade preparation Sangrovit-RS® as a source of sanguinarine (SG), chelerythrine (CH) and protropine (PA) standardized to 0.15% w/w SG, using feedlot cattle under a high-grain diet as an inflammatory model for gastrointestinal system. The samples of forestomachs were evaluated and graded using scores ranging from zero (0) to three (3) obtained at light-microscopic fields of 400X. Parameters such as inflammation, hydropic degeneration, hyperkeratosis, and vesicle formation were accessed in the different layers of the tissues, considering the severity and dispersion of the microscopic lesions. The soft tissues such as the abomasum, small intestine, cecum and colon had their total amount of inflammatory cells counted at light-microscopic fields of 200X. The rumen of the SG-CH-PRO-treated group showed a significant reduction in the epithelial hydropic degeneration scores (p ? 0.001) and lamina propria inflammation (p ? 0.001).The reticulum had a similar reduction in scores of epithelial (p ? 0.002) and stratum corneum hydropic degeneration (p ? 0.001), hyperkeratosis (p ? 0.002) and inflammation in lamina propria (p ? 0.001) and epithelium (p ? 0.002). The omasum had no significant differences. All non-keratinized tissues, except for ileum, had a significant decrease (p ? 0.001) in the total counting of inflammatory cells. In this trial, the feedlot cattle feed with high grain diet and treated with isoquinolone alkaloids expressed lesions that indicate ameliorations and worsening’s. Ameliorating effects of the alkaloids were better demonstrated in tissues with reduced or no corneal layer in the mucosa and in the absence of a lipopolysaccharides rich acidic environment reinforcing the notion of the topic action, the dependence of the media pH and the time of exposure modulating the pharmacological mechanisms of these alkaloids. The observed cytolytic (oncolysis) effect in epithelial forestomachs cells under low pH values, worsening the osmotic status, should be considered before clinical applications.As dietas para bovinos confinados possuem alta quantidade de alimentos energéticos com menor quantidade de alimentos volumosos (fibras), favorecendo a indução de acidose ruminal subaguda com formação de lesões gastrointestinais e sintomas clínicos deletérios. Recentes trabalhos têm demonstrado alguma amenização desta condição pelo uso de alcaloides isoquinolínicos encontrados na Macleaya cordata (Papaveraceae) tais como a sanguinarina e a cheleretrina, os quais tem demonstrado efeitos anti-inflamatórios, antimicrobianos e imuno modulatórios em humanos e animais. O objetivo deste estudo foi, através da histopatologia e de um sistema de escores, avaliar as diferenças entre um grupo não tratado e um grupo tratado com alcaloides isoquinolínicos, presentes na formulação Sangrovit RS® como fonte de sanguinarina (SG), chelerethrina (CH) and protropina (PA) padronizadas em 0,15 % w/w SG, usando bovinos em confinamento recebendo uma dieta rica em grãos como modelo inflamatório para o sistema gastrointestinal. Amostras dos pré-estômagos foram avaliadas por escores variando de zero a três, obtidos por microscopia de luz em diferentes campos em aumentos de 400X. Inflamação, degeneração hidrópica, hiperqueratose e formação de vesículas foram avaliadas nas diferentes camadas dos pré-estômagos tendo em vista a intensidade e a extensão das lesões. Tecidos não queratinizados como abomaso e intestino delgado, ceco e cólon tiveram seu total de células inflamatórias contadas por microscopia de luz em diferentes campos com aumentos de 200X. No rumem do grupo tratado houve uma redução significante no número de campos contendo degeneração hidrópica epitelial (p ? 0,001) e inflamação de lâmina própria (p ? 0,001). O retículo teve uma redução similar nos escores scores de degeneração hidrópica epitelial (p ? 0.002), degeneração hidrópica no estrato córneo (p ? 0.001), hiperqueratose (p ? 0.002) e inflamação na lamina própria (p ? 0.001) e inflamação epitelial (p ? 0.002). Não foram encontradas diferenças significantes no omaso. Todos os tecidos não queratinizados, exceto pelo íleo, tiveram uma redução significativa (p ? 0.001) no total de células inflamatórias. Neste experimento, bovinos em confinamento recebendo dieta rica em grãos e tratados com alcaloides isoquinolínicos tiveram lesões que tiveram melhoras e pioras. Os efeitos de melhora foram melhor demonstrados em tecidos sem camada córnea e na ausência de um meio ácido rico em lipopolisacarídeos, reforçando a noção da ação tópica, da dependência do pH do meio e do tempo de exposição modulando os mecanismos farmacológicos destes alcalóides. O efeito citolítico (oncólise) sobre células epiteliais de pré-estômagos em baixos valores de pH, piorando o estado osmótico das células, deveria ser considerado antes das aplicações clínicas.UEL2020-06-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResaerchPesquisaapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3607310.5433/1679-0359.2020v41n5p1567Semina: Ciências Agrárias; Vol. 41 No. 5 (2020); 1567-1580Semina: Ciências Agrárias; v. 41 n. 5 (2020); 1567-15801679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36073/27938Copyright (c) 2020 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessSanches, Adrien Wilhelm DilgerMontiani-Ferreira, FabianoSantin, ElizabethNeumann, MikaelReck, Angela MariaBertagnon, Heloisa GodoiPachaly, José Ricardo2022-10-07T15:42:55Zoai:ojs.pkp.sfu.ca:article/36073Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-07T15:42:55Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Isoquinolone alkaloids mitigate microscopic digestive tract lesions induced by sub-acute ruminal acidosis (SARA) in feedlot cattle Alcaloides isoquinolínicos atenuam lesões microscópicas digestivas induzidas por acidose ruminal subaguda em bovinos confinados |
title |
Isoquinolone alkaloids mitigate microscopic digestive tract lesions induced by sub-acute ruminal acidosis (SARA) in feedlot cattle |
spellingShingle |
Isoquinolone alkaloids mitigate microscopic digestive tract lesions induced by sub-acute ruminal acidosis (SARA) in feedlot cattle Sanches, Adrien Wilhelm Dilger Chelerythrine Na K - ATPase pump Ruminitis Sanguinarine. Bomba Na K - ATPase Queleritrina Rumenitis Sanguinarina. |
title_short |
Isoquinolone alkaloids mitigate microscopic digestive tract lesions induced by sub-acute ruminal acidosis (SARA) in feedlot cattle |
title_full |
Isoquinolone alkaloids mitigate microscopic digestive tract lesions induced by sub-acute ruminal acidosis (SARA) in feedlot cattle |
title_fullStr |
Isoquinolone alkaloids mitigate microscopic digestive tract lesions induced by sub-acute ruminal acidosis (SARA) in feedlot cattle |
title_full_unstemmed |
Isoquinolone alkaloids mitigate microscopic digestive tract lesions induced by sub-acute ruminal acidosis (SARA) in feedlot cattle |
title_sort |
Isoquinolone alkaloids mitigate microscopic digestive tract lesions induced by sub-acute ruminal acidosis (SARA) in feedlot cattle |
author |
Sanches, Adrien Wilhelm Dilger |
author_facet |
Sanches, Adrien Wilhelm Dilger Montiani-Ferreira, Fabiano Santin, Elizabeth Neumann, Mikael Reck, Angela Maria Bertagnon, Heloisa Godoi Pachaly, José Ricardo |
author_role |
author |
author2 |
Montiani-Ferreira, Fabiano Santin, Elizabeth Neumann, Mikael Reck, Angela Maria Bertagnon, Heloisa Godoi Pachaly, José Ricardo |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Sanches, Adrien Wilhelm Dilger Montiani-Ferreira, Fabiano Santin, Elizabeth Neumann, Mikael Reck, Angela Maria Bertagnon, Heloisa Godoi Pachaly, José Ricardo |
dc.subject.por.fl_str_mv |
Chelerythrine Na K - ATPase pump Ruminitis Sanguinarine. Bomba Na K - ATPase Queleritrina Rumenitis Sanguinarina. |
topic |
Chelerythrine Na K - ATPase pump Ruminitis Sanguinarine. Bomba Na K - ATPase Queleritrina Rumenitis Sanguinarina. |
description |
Feedlot cattle is submitted to a diet rich in energy and reduced in fibres that induces the sub-acute ruminal acidosis (SARA) with its lesions and clinical signs. Recent studies have demonstrated some amelioration of this condition by the use of isoquinolone alkaloids found in Macleaya cordata (Papaveraceae) such as Sanguinarine and Chelerythrine. These compounds have demonstrated antimicrobial, anti-in?ammatory and immune-modulatory effects in both humans and animals The aim on this study, using histopathology and a score system, was to evaluate the differences between a non-treated and a treated group feed with these alkaloids, present in trade preparation Sangrovit-RS® as a source of sanguinarine (SG), chelerythrine (CH) and protropine (PA) standardized to 0.15% w/w SG, using feedlot cattle under a high-grain diet as an inflammatory model for gastrointestinal system. The samples of forestomachs were evaluated and graded using scores ranging from zero (0) to three (3) obtained at light-microscopic fields of 400X. Parameters such as inflammation, hydropic degeneration, hyperkeratosis, and vesicle formation were accessed in the different layers of the tissues, considering the severity and dispersion of the microscopic lesions. The soft tissues such as the abomasum, small intestine, cecum and colon had their total amount of inflammatory cells counted at light-microscopic fields of 200X. The rumen of the SG-CH-PRO-treated group showed a significant reduction in the epithelial hydropic degeneration scores (p ? 0.001) and lamina propria inflammation (p ? 0.001).The reticulum had a similar reduction in scores of epithelial (p ? 0.002) and stratum corneum hydropic degeneration (p ? 0.001), hyperkeratosis (p ? 0.002) and inflammation in lamina propria (p ? 0.001) and epithelium (p ? 0.002). The omasum had no significant differences. All non-keratinized tissues, except for ileum, had a significant decrease (p ? 0.001) in the total counting of inflammatory cells. In this trial, the feedlot cattle feed with high grain diet and treated with isoquinolone alkaloids expressed lesions that indicate ameliorations and worsening’s. Ameliorating effects of the alkaloids were better demonstrated in tissues with reduced or no corneal layer in the mucosa and in the absence of a lipopolysaccharides rich acidic environment reinforcing the notion of the topic action, the dependence of the media pH and the time of exposure modulating the pharmacological mechanisms of these alkaloids. The observed cytolytic (oncolysis) effect in epithelial forestomachs cells under low pH values, worsening the osmotic status, should be considered before clinical applications. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-06-17 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Resaerch Pesquisa |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36073 10.5433/1679-0359.2020v41n5p1567 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36073 |
identifier_str_mv |
10.5433/1679-0359.2020v41n5p1567 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36073/27938 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 41 No. 5 (2020); 1567-1580 Semina: Ciências Agrárias; v. 41 n. 5 (2020); 1567-1580 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306081447444480 |