Comparative study between the hemocytometric and spectrophotometric methods for measuring the sperm concentration of young Nelore bulls
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Semina. Ciências Agrárias (Online) |
| Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/27072 |
Resumo: | Determination of sperm concentration using the Neubauer chamber (hemocytometric method) is a direct method for counting cells and also the most reliable. However, the process is time-consuming rendering it the least practical method when large numbers of ejaculates need to be processed. The spectrophotometer measures sperm concentrations as optical density and its main advantages are practicality and speed. This paper aimed to compare the results between evaluators using the hemocytometric method and the spectrophotometer for measuring sperm concentrations in young Nelore bulls. In total, 73 ejaculations from 20 young Nelore bulls were collected by electroejaculation. After soundness examination, 10 µL of the semen was diluted in 2 mL saline formaldehyde for measuring the sperm concentration per mL by the hemocytometric method (measured by three different evaluators) and the spectrophotometer method at 550 nm wavelength. No differences were detected in the results of sperm concentration measurements per mL among the evaluators using the hemocytometric method and the spectrophotometer (P > 0.05). The intraclass correlation was high (0.9), showing high replicability among the evaluator measurements. These results demonstrate that measurements performed using the spectrophotometer are reliable and can substitute the hemocytometric method in future for performing sperm concentration measurements in young Nelore bulls, thus improving and standardizing the techniques used in andrology laboratories. |
| id |
UEL-11_3a99d188ad9c47bf437d2a08b0f04bcb |
|---|---|
| oai_identifier_str |
oai:ojs.pkp.sfu.ca:article/27072 |
| network_acronym_str |
UEL-11 |
| network_name_str |
Semina. Ciências Agrárias (Online) |
| repository_id_str |
|
| spelling |
Comparative study between the hemocytometric and spectrophotometric methods for measuring the sperm concentration of young Nelore bullsEstudo comparativo entre o método hematocitométrico e o uso do espectrofotômetro para mensurar a concentração espermática de touros jovens da raça NeloreAbsorbanceAndrologyBos indicusNeubauer chamberSemen.AbsorbânciaAndrologiaBos indicusCâmara de NeubauerSêmen.Determination of sperm concentration using the Neubauer chamber (hemocytometric method) is a direct method for counting cells and also the most reliable. However, the process is time-consuming rendering it the least practical method when large numbers of ejaculates need to be processed. The spectrophotometer measures sperm concentrations as optical density and its main advantages are practicality and speed. This paper aimed to compare the results between evaluators using the hemocytometric method and the spectrophotometer for measuring sperm concentrations in young Nelore bulls. In total, 73 ejaculations from 20 young Nelore bulls were collected by electroejaculation. After soundness examination, 10 µL of the semen was diluted in 2 mL saline formaldehyde for measuring the sperm concentration per mL by the hemocytometric method (measured by three different evaluators) and the spectrophotometer method at 550 nm wavelength. No differences were detected in the results of sperm concentration measurements per mL among the evaluators using the hemocytometric method and the spectrophotometer (P > 0.05). The intraclass correlation was high (0.9), showing high replicability among the evaluator measurements. These results demonstrate that measurements performed using the spectrophotometer are reliable and can substitute the hemocytometric method in future for performing sperm concentration measurements in young Nelore bulls, thus improving and standardizing the techniques used in andrology laboratories.A determinação da concentração espermática pela câmara de Neubauer (método hematocitométrico) é um método direto para contar células, e também é o mais confiável. Entretanto, o processo é demorado, o que o torna um método menos prático quando a quantidade de ejaculados a ser processado é muito grande. O espectrofotômetro é um dispositivo que mede a concentração de espermatozoides através da densidade óptica, e as suas principais vantagens são a sua praticidade e sua velocidade. O objetivo deste trabalho foi comparar os resultados entre os avaliadores no método hematocitométrico e o espectrofotômetro para medir a concentração de espermatozoides no ejaculado de touros jovens da raça Nelore. Um total de 73 ejaculações de 20 touros jovens da raça Nelore foram coletados por meio de eletroejaculação. Após exame andrológico, 10µL do sémen foram diluídos em 2 mL de solução salina de formaldeído para medir a concentração de espermatozoides por mL, utilizando o método hematocitométrico (medido por três diferentes avaliadores) e utilizando o método do espectrofotómetro com comprimento de onda de 550 nm. Não foram detectadas diferenças nos resultados de medição de concentração de espermatozoides por mL entre os avaliadores medidos pelo método hematocitométrico e espectrofotômetro (P > 0,05). A correlação intraclasse foi alta (0,9), mostrando uma alta replicabilidade entre as medidas das avaliações. Com os resultados do presente experimento, pode-se afirmar que a mensuração realizada pelo espectrofotômetro é confiável, e pode, no futuro, substituir o método hematocitométrico para realizar as mensurações de concentração de espermatozoides no ejaculado de touros jovens da raça Nelore, melhorando e padronizando as técnicas usadas em laboratórios andrologia.UEL2017-11-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionExperimentação animalapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2707210.5433/1679-0359.2017v38n6p3605Semina: Ciências Agrárias; Vol. 38 No. 6 (2017); 3605-3612Semina: Ciências Agrárias; v. 38 n. 6 (2017); 3605-36121679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/27072/22267Copyright (c) 2017 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessCardeal, CléberAlberton, Luiz RomuloBoscarato, André GiarolaRocha, Mirelly VitalinaMarchetti, Lucas Giovanni LimaMacedo, Gustavo GuerinoLourenço, Emerson Luiz BotelhoMartins, Leonardo Franco2022-10-21T12:55:25Zoai:ojs.pkp.sfu.ca:article/27072Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-21T12:55:25Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
| dc.title.none.fl_str_mv |
Comparative study between the hemocytometric and spectrophotometric methods for measuring the sperm concentration of young Nelore bulls Estudo comparativo entre o método hematocitométrico e o uso do espectrofotômetro para mensurar a concentração espermática de touros jovens da raça Nelore |
| title |
Comparative study between the hemocytometric and spectrophotometric methods for measuring the sperm concentration of young Nelore bulls |
| spellingShingle |
Comparative study between the hemocytometric and spectrophotometric methods for measuring the sperm concentration of young Nelore bulls Cardeal, Cléber Absorbance Andrology Bos indicus Neubauer chamber Semen. Absorbância Andrologia Bos indicus Câmara de Neubauer Sêmen. |
| title_short |
Comparative study between the hemocytometric and spectrophotometric methods for measuring the sperm concentration of young Nelore bulls |
| title_full |
Comparative study between the hemocytometric and spectrophotometric methods for measuring the sperm concentration of young Nelore bulls |
| title_fullStr |
Comparative study between the hemocytometric and spectrophotometric methods for measuring the sperm concentration of young Nelore bulls |
| title_full_unstemmed |
Comparative study between the hemocytometric and spectrophotometric methods for measuring the sperm concentration of young Nelore bulls |
| title_sort |
Comparative study between the hemocytometric and spectrophotometric methods for measuring the sperm concentration of young Nelore bulls |
| author |
Cardeal, Cléber |
| author_facet |
Cardeal, Cléber Alberton, Luiz Romulo Boscarato, André Giarola Rocha, Mirelly Vitalina Marchetti, Lucas Giovanni Lima Macedo, Gustavo Guerino Lourenço, Emerson Luiz Botelho Martins, Leonardo Franco |
| author_role |
author |
| author2 |
Alberton, Luiz Romulo Boscarato, André Giarola Rocha, Mirelly Vitalina Marchetti, Lucas Giovanni Lima Macedo, Gustavo Guerino Lourenço, Emerson Luiz Botelho Martins, Leonardo Franco |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Cardeal, Cléber Alberton, Luiz Romulo Boscarato, André Giarola Rocha, Mirelly Vitalina Marchetti, Lucas Giovanni Lima Macedo, Gustavo Guerino Lourenço, Emerson Luiz Botelho Martins, Leonardo Franco |
| dc.subject.por.fl_str_mv |
Absorbance Andrology Bos indicus Neubauer chamber Semen. Absorbância Andrologia Bos indicus Câmara de Neubauer Sêmen. |
| topic |
Absorbance Andrology Bos indicus Neubauer chamber Semen. Absorbância Andrologia Bos indicus Câmara de Neubauer Sêmen. |
| description |
Determination of sperm concentration using the Neubauer chamber (hemocytometric method) is a direct method for counting cells and also the most reliable. However, the process is time-consuming rendering it the least practical method when large numbers of ejaculates need to be processed. The spectrophotometer measures sperm concentrations as optical density and its main advantages are practicality and speed. This paper aimed to compare the results between evaluators using the hemocytometric method and the spectrophotometer for measuring sperm concentrations in young Nelore bulls. In total, 73 ejaculations from 20 young Nelore bulls were collected by electroejaculation. After soundness examination, 10 µL of the semen was diluted in 2 mL saline formaldehyde for measuring the sperm concentration per mL by the hemocytometric method (measured by three different evaluators) and the spectrophotometer method at 550 nm wavelength. No differences were detected in the results of sperm concentration measurements per mL among the evaluators using the hemocytometric method and the spectrophotometer (P > 0.05). The intraclass correlation was high (0.9), showing high replicability among the evaluator measurements. These results demonstrate that measurements performed using the spectrophotometer are reliable and can substitute the hemocytometric method in future for performing sperm concentration measurements in young Nelore bulls, thus improving and standardizing the techniques used in andrology laboratories. |
| publishDate |
2017 |
| dc.date.none.fl_str_mv |
2017-11-23 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Experimentação animal |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/27072 10.5433/1679-0359.2017v38n6p3605 |
| url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/27072 |
| identifier_str_mv |
10.5433/1679-0359.2017v38n6p3605 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/27072/22267 |
| dc.rights.driver.fl_str_mv |
Copyright (c) 2017 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
Copyright (c) 2017 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
UEL |
| publisher.none.fl_str_mv |
UEL |
| dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 38 No. 6 (2017); 3605-3612 Semina: Ciências Agrárias; v. 38 n. 6 (2017); 3605-3612 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
| instname_str |
Universidade Estadual de Londrina (UEL) |
| instacron_str |
UEL |
| institution |
UEL |
| reponame_str |
Semina. Ciências Agrárias (Online) |
| collection |
Semina. Ciências Agrárias (Online) |
| repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
| repository.mail.fl_str_mv |
semina.agrarias@uel.br |
| _version_ |
1799306076917596160 |