Identification of Leishmania spp. in horses and a dog from rural areas of Uruguaiana, Rio Grande do Sul, Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Semina. Ciências Agrárias (Online) |
| Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38423 |
Resumo: | Visceral leishmaniasis (VL) is a zoonosis caused by the protozoan of the genus Leishmania. The disease is transmitted by the bite of a sand fly vector. Although the main reservoirs are dogs, other hosts can be infected and may play this role. Rio Grande do Sul western region, located on the triple border of Brazil-Uruguay-Argentina, represents a VL transmission area. The goal of the present study was to identify Leishmania spp. infection in animals from rural areas of Uruguaiana, Rio Grande do Sul. Nine farms in the Uruguaiana municipality, Rio Grande do Sul state, were included. Peripheral blood samples were collected from 113 animals (canine [n=22], equine [n=91]) for detection of Leishmania spp. DNA was isolated and polymerase chain reaction was performed. Eight (7%) animals with Leishmania spp. infection were detected on two farms in the same geographical area, seven of which were horses and one was canine, all of which were asymptomatic. To investigate the species of Leishmania, one of the positive equine samples was subjected to direct sequencing, which confirmed the presence of L. infantum genetic material. Results of this study confirm the presence of L. infantum-infected animals in rural areas of Uruguaiana, and provide evidence supporting further investigation of risk factors for dissemination in such areas. |
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Identification of Leishmania spp. in horses and a dog from rural areas of Uruguaiana, Rio Grande do Sul, BrazilIdentificação de Leishmania spp. em equinos e um cão da área rural de Uruguaiana, Rio Grande do Sul, BrasilDomestic animalsLeishmaniasisPCRPublic healthWestern border.Animais domésticos. LeishmaniosePCRSaúde públicaFronteira oeste.Visceral leishmaniasis (VL) is a zoonosis caused by the protozoan of the genus Leishmania. The disease is transmitted by the bite of a sand fly vector. Although the main reservoirs are dogs, other hosts can be infected and may play this role. Rio Grande do Sul western region, located on the triple border of Brazil-Uruguay-Argentina, represents a VL transmission area. The goal of the present study was to identify Leishmania spp. infection in animals from rural areas of Uruguaiana, Rio Grande do Sul. Nine farms in the Uruguaiana municipality, Rio Grande do Sul state, were included. Peripheral blood samples were collected from 113 animals (canine [n=22], equine [n=91]) for detection of Leishmania spp. DNA was isolated and polymerase chain reaction was performed. Eight (7%) animals with Leishmania spp. infection were detected on two farms in the same geographical area, seven of which were horses and one was canine, all of which were asymptomatic. To investigate the species of Leishmania, one of the positive equine samples was subjected to direct sequencing, which confirmed the presence of L. infantum genetic material. Results of this study confirm the presence of L. infantum-infected animals in rural areas of Uruguaiana, and provide evidence supporting further investigation of risk factors for dissemination in such areas.A leishmaniose visceral é uma zoonose causada por protozoários do gênero Leishmania. A doença é transmitida pela picada de um vetor flebotomíneo. Os principais reservatórios são cães, porém outros mamíferos podem se infectar e desempenhar esse papel. A região oeste do Rio Grande do Sul, localizada na tríplice fronteira Brasil-Uruguai-Argentina, representa uma área de transmissão para a leishmaniose visceral. O objetivo deste estudo foi identificar a infecção por Leishmania spp. em animais de áreas rurais de Uruguaiana, Rio Grande do Sul. Nove fazendas do município de Uruguaiana, no Rio Grande do Sul, foram incluídas. O sangue periférico foi coletado de 113 animais, caninos (n = 22) e equinos (n = 91), para a pesquisa de DNA de Leishmania spp. por reação em cadeia da polimerase (PCR). Oito animais infectados por Leishmania spp. (7%) foram detectados em duas fazendas na mesma área geográfica, sete equinos e um canino. Todos eles eram assintomáticos. Para investigar as espécies de Leishmania, uma das amostras positivas da espécie equina, também foi submetida ao sequenciamento e a presença de material genético de L. infantum foi confirmada. Este estudo garante a existência de animais infectados por Leishmania infantum nas áreas rurais de Uruguaiana e evidencia a importância de estudos para investigar os fatores de risco de disseminação nestes locais.UEL2020-09-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3842310.5433/1679-0359.2020v41n6p2687Semina: Ciências Agrárias; Vol. 41 No. 6 (2020); 2687-2694Semina: Ciências Agrárias; v. 41 n. 6 (2020); 2687-26941679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38423/28070Copyright (c) 2020 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessPradella, Gabriela DöwichEscobar, Taiane AcunhaDuarte, Claudia AcostaLübeck, IrinaGóss, Geórgia CamargoLagreca, Luís Felipe JaenischHeckler, Gabriel FeixCamargo, Lilian PintoRomero, Bruno Gostinski2022-10-07T11:41:57Zoai:ojs.pkp.sfu.ca:article/38423Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-07T11:41:57Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
| dc.title.none.fl_str_mv |
Identification of Leishmania spp. in horses and a dog from rural areas of Uruguaiana, Rio Grande do Sul, Brazil Identificação de Leishmania spp. em equinos e um cão da área rural de Uruguaiana, Rio Grande do Sul, Brasil |
| title |
Identification of Leishmania spp. in horses and a dog from rural areas of Uruguaiana, Rio Grande do Sul, Brazil |
| spellingShingle |
Identification of Leishmania spp. in horses and a dog from rural areas of Uruguaiana, Rio Grande do Sul, Brazil Pradella, Gabriela Döwich Domestic animals Leishmaniasis PCR Public health Western border. Animais domésticos. Leishmaniose PCR Saúde pública Fronteira oeste. |
| title_short |
Identification of Leishmania spp. in horses and a dog from rural areas of Uruguaiana, Rio Grande do Sul, Brazil |
| title_full |
Identification of Leishmania spp. in horses and a dog from rural areas of Uruguaiana, Rio Grande do Sul, Brazil |
| title_fullStr |
Identification of Leishmania spp. in horses and a dog from rural areas of Uruguaiana, Rio Grande do Sul, Brazil |
| title_full_unstemmed |
Identification of Leishmania spp. in horses and a dog from rural areas of Uruguaiana, Rio Grande do Sul, Brazil |
| title_sort |
Identification of Leishmania spp. in horses and a dog from rural areas of Uruguaiana, Rio Grande do Sul, Brazil |
| author |
Pradella, Gabriela Döwich |
| author_facet |
Pradella, Gabriela Döwich Escobar, Taiane Acunha Duarte, Claudia Acosta Lübeck, Irina Góss, Geórgia Camargo Lagreca, Luís Felipe Jaenisch Heckler, Gabriel Feix Camargo, Lilian Pinto Romero, Bruno Gostinski |
| author_role |
author |
| author2 |
Escobar, Taiane Acunha Duarte, Claudia Acosta Lübeck, Irina Góss, Geórgia Camargo Lagreca, Luís Felipe Jaenisch Heckler, Gabriel Feix Camargo, Lilian Pinto Romero, Bruno Gostinski |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Pradella, Gabriela Döwich Escobar, Taiane Acunha Duarte, Claudia Acosta Lübeck, Irina Góss, Geórgia Camargo Lagreca, Luís Felipe Jaenisch Heckler, Gabriel Feix Camargo, Lilian Pinto Romero, Bruno Gostinski |
| dc.subject.por.fl_str_mv |
Domestic animals Leishmaniasis PCR Public health Western border. Animais domésticos. Leishmaniose PCR Saúde pública Fronteira oeste. |
| topic |
Domestic animals Leishmaniasis PCR Public health Western border. Animais domésticos. Leishmaniose PCR Saúde pública Fronteira oeste. |
| description |
Visceral leishmaniasis (VL) is a zoonosis caused by the protozoan of the genus Leishmania. The disease is transmitted by the bite of a sand fly vector. Although the main reservoirs are dogs, other hosts can be infected and may play this role. Rio Grande do Sul western region, located on the triple border of Brazil-Uruguay-Argentina, represents a VL transmission area. The goal of the present study was to identify Leishmania spp. infection in animals from rural areas of Uruguaiana, Rio Grande do Sul. Nine farms in the Uruguaiana municipality, Rio Grande do Sul state, were included. Peripheral blood samples were collected from 113 animals (canine [n=22], equine [n=91]) for detection of Leishmania spp. DNA was isolated and polymerase chain reaction was performed. Eight (7%) animals with Leishmania spp. infection were detected on two farms in the same geographical area, seven of which were horses and one was canine, all of which were asymptomatic. To investigate the species of Leishmania, one of the positive equine samples was subjected to direct sequencing, which confirmed the presence of L. infantum genetic material. Results of this study confirm the presence of L. infantum-infected animals in rural areas of Uruguaiana, and provide evidence supporting further investigation of risk factors for dissemination in such areas. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-09-19 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38423 10.5433/1679-0359.2020v41n6p2687 |
| url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38423 |
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10.5433/1679-0359.2020v41n6p2687 |
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eng |
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eng |
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https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38423/28070 |
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Copyright (c) 2020 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
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Copyright (c) 2020 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
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openAccess |
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application/pdf |
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UEL |
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UEL |
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Semina: Ciências Agrárias; Vol. 41 No. 6 (2020); 2687-2694 Semina: Ciências Agrárias; v. 41 n. 6 (2020); 2687-2694 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
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Semina. Ciências Agrárias (Online) |
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Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
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semina.agrarias@uel.br |
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1799306082295742464 |