Comparative study of two serological tests for detection of anti-Theileria equi antibodies in horses

Detalhes bibliográficos
Autor(a) principal: Vieira, Thállitha Samih Wischral Jayme
Data de Publicação: 2015
Outros Autores: Valente, Jessica Damiana Marinho, Silva, Nayara Bezerra, Sicupira, Patrícia Mara Lopes, Barros-Filho, Ivan Roque, Biondo, Alexander Welker, Vieira, Rafael Felipe da Costa, Vidotto, Odilon
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/23388
Resumo: Theileria equi is the causative agent of worldwide piroplasmosis, an important tick-borne disease of equids associated to a lifetime carrier state of infected horses. Since rapid, accessible and reliable tests have been posted as a challenge for clinicians, the aim of the present study was to verify the agreement between an in-house immunofluorescence antibody assay (IFA) and a commercial competitive inhibition enzyme-linked immunosorbent assay (cELISA) in a population of 198 carthorses from Southern Brazil. The horse seroreactivity for T. equi revealed 152/198 (76.8%) positive samples by IFA and 155/198 (78.3%) by cELISA. Using cELISA as gold standard, IFA has shown a sensitivity of 91.6% (95% CI = 86.18-95.03%) and specificity of 76.7% (95% CI = 62.26-86.85%), with a substantial degree of agreement (k = 0.8445). In conclusion, the in-house IFA may be used as a screening test for diagnosis of equine piroplasmosis.
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spelling Comparative study of two serological tests for detection of anti-Theileria equi antibodies in horsesEstudo comparativo de dois testes sorológicos para detecção de anticorpos anti-Theileria equi em equinosPiroplasmosisEquidsSeroepidemiologyTheileriosis.IroplasmoseEqüídeosSoroepidemiologiaTeileriose.Theileria equi is the causative agent of worldwide piroplasmosis, an important tick-borne disease of equids associated to a lifetime carrier state of infected horses. Since rapid, accessible and reliable tests have been posted as a challenge for clinicians, the aim of the present study was to verify the agreement between an in-house immunofluorescence antibody assay (IFA) and a commercial competitive inhibition enzyme-linked immunosorbent assay (cELISA) in a population of 198 carthorses from Southern Brazil. The horse seroreactivity for T. equi revealed 152/198 (76.8%) positive samples by IFA and 155/198 (78.3%) by cELISA. Using cELISA as gold standard, IFA has shown a sensitivity of 91.6% (95% CI = 86.18-95.03%) and specificity of 76.7% (95% CI = 62.26-86.85%), with a substantial degree of agreement (k = 0.8445). In conclusion, the in-house IFA may be used as a screening test for diagnosis of equine piroplasmosis.Theileria equi é o agente causador da piroplasmose no mundo, é uma importante doença transmitida por carrapatos nos equídeos em todo o mundo, e está associada ao estado de portador nos equinos infectados. Uma vez que testes rápidos, acessíveis e confiáveis são considerados um desafio para os clínicos, o objetivo do presente estudo foi avaliar a concordância entre os testes de imunofluorescência indireta (RIFI) e um ensaio imunoenzimático por competição comercial (cELISA) em uma população de 198 equinos de tração na região Sul do Brasil. A sororreatividade dos cavalos para T. equi mostrou 152/198 (76.8%) amostras positivas para RIFI e 155/198 (78.3%) amostras positivas para cELISA. Utilizando o cELISA como padrão ouro, a RIFI demonstrou sensibilidade de 91.6% (95% CI = 86.18-95.03%) e especificidade de 76.7% (95% CI = 62.26-86.85%), com uma concordância de grau substancial (k = 0.8445). Em conclusão, a RIFI pode ser utilizada como um teste de triagem para o diagnóstico de piroplasmose equina.UEL2015-12-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionshort communicationAvaliado por paresapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2338810.5433/1679-0359.2015v36n6Supl2p4361Semina: Ciências Agrárias; Vol. 36 No. 6Supl2 (2015); 4361-4364Semina: Ciências Agrárias; v. 36 n. 6Supl2 (2015); 4361-43641679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/23388/17558Vieira, Thállitha Samih Wischral JaymeValente, Jessica Damiana MarinhoSilva, Nayara BezerraSicupira, Patrícia Mara LopesBarros-Filho, Ivan RoqueBiondo, Alexander WelkerVieira, Rafael Felipe da CostaVidotto, Odiloninfo:eu-repo/semantics/openAccess2015-12-17T16:15:00Zoai:ojs.pkp.sfu.ca:article/23388Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2015-12-17T16:15Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Comparative study of two serological tests for detection of anti-Theileria equi antibodies in horses
Estudo comparativo de dois testes sorológicos para detecção de anticorpos anti-Theileria equi em equinos
title Comparative study of two serological tests for detection of anti-Theileria equi antibodies in horses
spellingShingle Comparative study of two serological tests for detection of anti-Theileria equi antibodies in horses
Vieira, Thállitha Samih Wischral Jayme
Piroplasmosis
Equids
Seroepidemiology
Theileriosis.
Iroplasmose
Eqüídeos
Soroepidemiologia
Teileriose.
title_short Comparative study of two serological tests for detection of anti-Theileria equi antibodies in horses
title_full Comparative study of two serological tests for detection of anti-Theileria equi antibodies in horses
title_fullStr Comparative study of two serological tests for detection of anti-Theileria equi antibodies in horses
title_full_unstemmed Comparative study of two serological tests for detection of anti-Theileria equi antibodies in horses
title_sort Comparative study of two serological tests for detection of anti-Theileria equi antibodies in horses
author Vieira, Thállitha Samih Wischral Jayme
author_facet Vieira, Thállitha Samih Wischral Jayme
Valente, Jessica Damiana Marinho
Silva, Nayara Bezerra
Sicupira, Patrícia Mara Lopes
Barros-Filho, Ivan Roque
Biondo, Alexander Welker
Vieira, Rafael Felipe da Costa
Vidotto, Odilon
author_role author
author2 Valente, Jessica Damiana Marinho
Silva, Nayara Bezerra
Sicupira, Patrícia Mara Lopes
Barros-Filho, Ivan Roque
Biondo, Alexander Welker
Vieira, Rafael Felipe da Costa
Vidotto, Odilon
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Vieira, Thállitha Samih Wischral Jayme
Valente, Jessica Damiana Marinho
Silva, Nayara Bezerra
Sicupira, Patrícia Mara Lopes
Barros-Filho, Ivan Roque
Biondo, Alexander Welker
Vieira, Rafael Felipe da Costa
Vidotto, Odilon
dc.subject.por.fl_str_mv Piroplasmosis
Equids
Seroepidemiology
Theileriosis.
Iroplasmose
Eqüídeos
Soroepidemiologia
Teileriose.
topic Piroplasmosis
Equids
Seroepidemiology
Theileriosis.
Iroplasmose
Eqüídeos
Soroepidemiologia
Teileriose.
description Theileria equi is the causative agent of worldwide piroplasmosis, an important tick-borne disease of equids associated to a lifetime carrier state of infected horses. Since rapid, accessible and reliable tests have been posted as a challenge for clinicians, the aim of the present study was to verify the agreement between an in-house immunofluorescence antibody assay (IFA) and a commercial competitive inhibition enzyme-linked immunosorbent assay (cELISA) in a population of 198 carthorses from Southern Brazil. The horse seroreactivity for T. equi revealed 152/198 (76.8%) positive samples by IFA and 155/198 (78.3%) by cELISA. Using cELISA as gold standard, IFA has shown a sensitivity of 91.6% (95% CI = 86.18-95.03%) and specificity of 76.7% (95% CI = 62.26-86.85%), with a substantial degree of agreement (k = 0.8445). In conclusion, the in-house IFA may be used as a screening test for diagnosis of equine piroplasmosis.
publishDate 2015
dc.date.none.fl_str_mv 2015-12-16
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
short communication
Avaliado por pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/23388
10.5433/1679-0359.2015v36n6Supl2p4361
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/23388
identifier_str_mv 10.5433/1679-0359.2015v36n6Supl2p4361
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/23388/17558
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 36 No. 6Supl2 (2015); 4361-4364
Semina: Ciências Agrárias; v. 36 n. 6Supl2 (2015); 4361-4364
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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