Effect of dietary antioxidant supplementation on reproductive characteristics of male broiler breeders during the post-peak production phase
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Semina. Ciências Agrárias (Online) |
| Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/23410 |
Resumo: | Because of their membrane constitution, sperm cells of male broiler breeders are prone to lipid peroxidation, which affects their fertilizing capacity. The aim of the present study was to evaluate the effects of antioxidant diet supplementation on the reproductive characteristics of 12 male broiler breeders (Cobb) older than 50 weeks. The roosters were randomly distributed in a completely randomized experimental design with two treatments (treatment 1: commercial feed; treatment 2: commercial feed supplemented with canthaxanthin, lycopene, and vitamin C) and 6 replicates. Semen samples were collected by abdominal massage, and then underwent evaluation of semen volume, sperm concentration, motility, vigor, and morphology. Morphometric analyses of testes, combs, and dewlaps were performed at 68 weeks. Testes samples were collected for morphometric analysis of seminiferous tubules and analysis of cellular proliferation in the germinal epithelium by using immunohistochemical staining with anti-proliferating cell nuclear antigen (anti-PCNA) antibodies. Statistical data analysis was performed using the SAS software (SAS, 2002). No significant effects of antioxidant supplementation were observed on semen characteristics (p > 0.05). A significant positive effect of the antioxidant blend was observed on the percentage of normal sperm, dewlap weight and width, and testes weight and length (p < 0.05). PCNA-positive cell counts and morphometry of the seminiferous tubules were not affected by the treatment. Dietary antioxidant supplementation may represent a nutritional tool to increase fertility in male broiler breeder flocks during the post-peak production phase. |
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Effect of dietary antioxidant supplementation on reproductive characteristics of male broiler breeders during the post-peak production phaseEfeito da suplementação dietética de antioxidantes sobre características reprodutivas de machos reprodutores de frangos de corte na fase pós pico de produçãoCanthaxanthinRoostersLycopeneLipid peroxidationVitamin C.CantaxantinaGalosLicopenoPeroxidação lipídicaVitamina C.Because of their membrane constitution, sperm cells of male broiler breeders are prone to lipid peroxidation, which affects their fertilizing capacity. The aim of the present study was to evaluate the effects of antioxidant diet supplementation on the reproductive characteristics of 12 male broiler breeders (Cobb) older than 50 weeks. The roosters were randomly distributed in a completely randomized experimental design with two treatments (treatment 1: commercial feed; treatment 2: commercial feed supplemented with canthaxanthin, lycopene, and vitamin C) and 6 replicates. Semen samples were collected by abdominal massage, and then underwent evaluation of semen volume, sperm concentration, motility, vigor, and morphology. Morphometric analyses of testes, combs, and dewlaps were performed at 68 weeks. Testes samples were collected for morphometric analysis of seminiferous tubules and analysis of cellular proliferation in the germinal epithelium by using immunohistochemical staining with anti-proliferating cell nuclear antigen (anti-PCNA) antibodies. Statistical data analysis was performed using the SAS software (SAS, 2002). No significant effects of antioxidant supplementation were observed on semen characteristics (p > 0.05). A significant positive effect of the antioxidant blend was observed on the percentage of normal sperm, dewlap weight and width, and testes weight and length (p < 0.05). PCNA-positive cell counts and morphometry of the seminiferous tubules were not affected by the treatment. Dietary antioxidant supplementation may represent a nutritional tool to increase fertility in male broiler breeder flocks during the post-peak production phase.Os espermatozoides de machos reprodutores de frango de corte são células propensas a peroxidação lipídica, dada à constituição biológica de sua membrana, influenciando a capacidade fertilizante do macho. O objetivo deste trabalho foi avaliar o efeito da suplementação dietética de antioxidantes sobre características reprodutivas de machos reprodutores de frangos de corte com idade superior a 50 semanas. Foram utilizados 12 galos Cobb de 50 semanas de idade distribuídos aleatoriamente em um delineamento inteiramente casualizado com 2 tratamentos (tratamento 1: ração comercial; tratamento 2: ração comercial suplementada de cantaxantina, licopeno e vitamina C) e 6 repetições. Amostras de sêmen foram coletadas pelo método de massagem abdominal e submetidas à avaliação do volume seminal, concentração, motilidade, vigor e morfologia espermática. Às 68 semanas de idade, amostras de testículo foram coletadas para determinação da morfometria tubular dos testículos e para análise da proliferação celular no epitélio germinativo através de imunohistoquímica anti-PCNA. A morfometria de testículos, cristas e barbelas foram analisadas nas 68 semanas de idade. Os dados foram submetidos à análise estatística utilizando-se o software SAS (SAS, 2002). Não foram observadas diferenças significativas (p > 0,05) com a adição dos antioxidantes sobre as características seminais. Houve efeito positivo (p < 0,05) do blend de antioxidantes no percentual de espermatozoides normais, no peso e largura das barbelas e no peso e comprimento dos testículos. A contagem de células PCNA-positivas e as medidas morfométrias dos túbulos seminíferos dos testículos dos galos não sofreram influência do tratamento. A adição de antioxidantes na dieta de machos reprodutores de frango de corte na fase póspico pode ser uma ferramenta nutricional para melhorar o índice de fertilidade dos lotes de matrizes de frangos de corte.UEL2016-09-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa Empírica de campoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2341010.5433/1679-0359.2016v37n4Supl1p2557Semina: Ciências Agrárias; Vol. 37 No. 4Supl1 (2016); 2557-2566Semina: Ciências Agrárias; v. 37 n. 4Supl1 (2016); 2557-25661679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/23410/19603http://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessTriques, Gustavo EugênioSchmidt, Joice MeriOro, Camila SouzaBordignon, Heloísa FialkoskiDonin, Daiane GüillichFernandes, Jovanir Inês Müller2022-11-30T15:24:10Zoai:ojs.pkp.sfu.ca:article/23410Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-11-30T15:24:10Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
| dc.title.none.fl_str_mv |
Effect of dietary antioxidant supplementation on reproductive characteristics of male broiler breeders during the post-peak production phase Efeito da suplementação dietética de antioxidantes sobre características reprodutivas de machos reprodutores de frangos de corte na fase pós pico de produção |
| title |
Effect of dietary antioxidant supplementation on reproductive characteristics of male broiler breeders during the post-peak production phase |
| spellingShingle |
Effect of dietary antioxidant supplementation on reproductive characteristics of male broiler breeders during the post-peak production phase Triques, Gustavo Eugênio Canthaxanthin Roosters Lycopene Lipid peroxidation Vitamin C. Cantaxantina Galos Licopeno Peroxidação lipídica Vitamina C. |
| title_short |
Effect of dietary antioxidant supplementation on reproductive characteristics of male broiler breeders during the post-peak production phase |
| title_full |
Effect of dietary antioxidant supplementation on reproductive characteristics of male broiler breeders during the post-peak production phase |
| title_fullStr |
Effect of dietary antioxidant supplementation on reproductive characteristics of male broiler breeders during the post-peak production phase |
| title_full_unstemmed |
Effect of dietary antioxidant supplementation on reproductive characteristics of male broiler breeders during the post-peak production phase |
| title_sort |
Effect of dietary antioxidant supplementation on reproductive characteristics of male broiler breeders during the post-peak production phase |
| author |
Triques, Gustavo Eugênio |
| author_facet |
Triques, Gustavo Eugênio Schmidt, Joice Meri Oro, Camila Souza Bordignon, Heloísa Fialkoski Donin, Daiane Güillich Fernandes, Jovanir Inês Müller |
| author_role |
author |
| author2 |
Schmidt, Joice Meri Oro, Camila Souza Bordignon, Heloísa Fialkoski Donin, Daiane Güillich Fernandes, Jovanir Inês Müller |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Triques, Gustavo Eugênio Schmidt, Joice Meri Oro, Camila Souza Bordignon, Heloísa Fialkoski Donin, Daiane Güillich Fernandes, Jovanir Inês Müller |
| dc.subject.por.fl_str_mv |
Canthaxanthin Roosters Lycopene Lipid peroxidation Vitamin C. Cantaxantina Galos Licopeno Peroxidação lipídica Vitamina C. |
| topic |
Canthaxanthin Roosters Lycopene Lipid peroxidation Vitamin C. Cantaxantina Galos Licopeno Peroxidação lipídica Vitamina C. |
| description |
Because of their membrane constitution, sperm cells of male broiler breeders are prone to lipid peroxidation, which affects their fertilizing capacity. The aim of the present study was to evaluate the effects of antioxidant diet supplementation on the reproductive characteristics of 12 male broiler breeders (Cobb) older than 50 weeks. The roosters were randomly distributed in a completely randomized experimental design with two treatments (treatment 1: commercial feed; treatment 2: commercial feed supplemented with canthaxanthin, lycopene, and vitamin C) and 6 replicates. Semen samples were collected by abdominal massage, and then underwent evaluation of semen volume, sperm concentration, motility, vigor, and morphology. Morphometric analyses of testes, combs, and dewlaps were performed at 68 weeks. Testes samples were collected for morphometric analysis of seminiferous tubules and analysis of cellular proliferation in the germinal epithelium by using immunohistochemical staining with anti-proliferating cell nuclear antigen (anti-PCNA) antibodies. Statistical data analysis was performed using the SAS software (SAS, 2002). No significant effects of antioxidant supplementation were observed on semen characteristics (p > 0.05). A significant positive effect of the antioxidant blend was observed on the percentage of normal sperm, dewlap weight and width, and testes weight and length (p < 0.05). PCNA-positive cell counts and morphometry of the seminiferous tubules were not affected by the treatment. Dietary antioxidant supplementation may represent a nutritional tool to increase fertility in male broiler breeder flocks during the post-peak production phase. |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016-09-02 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pesquisa Empírica de campo |
| format |
article |
| status_str |
publishedVersion |
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https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/23410 10.5433/1679-0359.2016v37n4Supl1p2557 |
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https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/23410 |
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10.5433/1679-0359.2016v37n4Supl1p2557 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
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https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/23410/19603 |
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http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by-nc/4.0 |
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openAccess |
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application/pdf |
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UEL |
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UEL |
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Semina: Ciências Agrárias; Vol. 37 No. 4Supl1 (2016); 2557-2566 Semina: Ciências Agrárias; v. 37 n. 4Supl1 (2016); 2557-2566 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
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Universidade Estadual de Londrina (UEL) |
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UEL |
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UEL |
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Semina. Ciências Agrárias (Online) |
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Semina. Ciências Agrárias (Online) |
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Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
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semina.agrarias@uel.br |
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1799306075030159360 |