In vitro effect of Eucalyptus globulus essential oil on Penicillium sp.

Detalhes bibliográficos
Autor(a) principal: Piatti, Andréia
Data de Publicação: 2011
Outros Autores: Schneider, Cristina Fernanda, Nozaki, Marcia Holanda
Tipo de documento: Artigo
Idioma: por
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1679-0359.2011v32n3p1033
Resumo: Penicillium sp. is the casual agent of moulds, considered the main citrus post-harvest disease, causing loss on the quality and quantity of marketable fruits. Besides economic loss, it can be included costs, transportation and fruit storage problems. The present work had the objective to verify the fungitoxic action of Eucalyptus globules essential oil on the in vitro control of Penicillium sp., evaluating mycelial growth, production and spores germination. The experiment was conduced on the Microbiology laboratory of PUC, Toledo campus, with eight treatments: 1%, 0,5%, 0,25%, 0,1%, 0,05% e 0,025% concentrations of eucalyptus oil added to PDA media (potato-dextrose-agar), negative control (PDA with addition of 40mg of azoxystrobin/L) and positive control (PDA media only). The experimental design was according to a completely randomized design, with five replicates each treatment. The eucalyptus essential oil inhibited significantly the growth in the 1%, 0,5% e 0,25% concentrations, not differing statistically from control with fungicide azoxystrobin. The treatments presented the same behavior for spores production. For spores germination, the 1% e 0,5% concentrations had better control than treatment with azoxystrobin and the concentration of 0,25%. Others oil concentrations did not present significative results on the evaluations, being statistically equal to positive control (PDA).
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spelling In vitro effect of Eucalyptus globulus essential oil on Penicillium sp.Efeito in vitro do óleo essencial de Eucalyptus globulus sobre o crescimento e desenvolvimento de Penicillium sp.FungusCitrusMouldsEucalyptusPost-harvest control.FungoCitrosPodridõesEucaliptoControle pós-colheita.Penicillium sp. is the casual agent of moulds, considered the main citrus post-harvest disease, causing loss on the quality and quantity of marketable fruits. Besides economic loss, it can be included costs, transportation and fruit storage problems. The present work had the objective to verify the fungitoxic action of Eucalyptus globules essential oil on the in vitro control of Penicillium sp., evaluating mycelial growth, production and spores germination. The experiment was conduced on the Microbiology laboratory of PUC, Toledo campus, with eight treatments: 1%, 0,5%, 0,25%, 0,1%, 0,05% e 0,025% concentrations of eucalyptus oil added to PDA media (potato-dextrose-agar), negative control (PDA with addition of 40mg of azoxystrobin/L) and positive control (PDA media only). The experimental design was according to a completely randomized design, with five replicates each treatment. The eucalyptus essential oil inhibited significantly the growth in the 1%, 0,5% e 0,25% concentrations, not differing statistically from control with fungicide azoxystrobin. The treatments presented the same behavior for spores production. For spores germination, the 1% e 0,5% concentrations had better control than treatment with azoxystrobin and the concentration of 0,25%. Others oil concentrations did not present significative results on the evaluations, being statistically equal to positive control (PDA).O fungo Penicillium sp. é o agente causal dos bolores, considerada a principal doença pós-colheita em citros, levando à perdas na qualidade e quantidade dos frutos comercializáveis. Além de perdas econômicas, uma vez que nas perdas pós-colheitas estão inclusos os custos, transporte e armazenagem de frutos. O presente trabalho teve por objetivo verificar a ação fungitóxica do óleo essencial de Eucalyptus globulus no controle in vitro de Penicillium sp., avaliando seu crescimento micelial, produção e a germinação de esporos. O experimento foi conduzido no laboratório de Microbiologia da PUC, campus Toledo, e constituiu-se de oito tratamentos, sendo: uso do óleo essencial de eucalipto nas concentrações de 1%, 0,5%, 0,25%, 0,1%, 0,05% e 0,025% adicionados ao meio de cultura BDA (Batata Dextrose-Ágar) autoclavado, testemunha negativa (meio de cultura com adição de 40 mg de ingrediente ativo azoxystrobin/L do fungicida) e testemunha positiva (apenas meio de cultura). O delineamento experimental utilizado foi inteiramente casualizado, com cinco repetições em cada tratamento. O óleo essencial de eucalipto inibiu o crescimento de forma significativa nas concentrações de 1%, 0,5% e 0,25%, não diferindo estatisticamente do controle com o fungicida azoxystrobin. Os tratamentos apresentaram o mesmo comportamento em relação a produção de esporos. Já na germinação de esporos, as concentrações de 1% e 0,5% obtiveram controle superior ao tratamento com azoxystrobin e a concentração de 0,25%. As demais concentrações de óleo não obtiveram resultados significativos nas avaliações, igualando-se estatisticamente a testemunha positiva (BDA).UEL2011-08-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1679-0359.2011v32n3p103310.5433/1679-0359.2011v32n3p1033Semina: Ciências Agrárias; Vol. 32 No. 3 (2011); 1033-1040Semina: Ciências Agrárias; v. 32 n. 3 (2011); 1033-10401679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1679-0359.2011v32n3p1033/8377Piatti, AndréiaSchneider, Cristina FernandaNozaki, Marcia Holandainfo:eu-repo/semantics/openAccess2015-11-19T18:37:50Zoai:ojs.pkp.sfu.ca:article/3119Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2015-11-19T18:37:50Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv In vitro effect of Eucalyptus globulus essential oil on Penicillium sp.
Efeito in vitro do óleo essencial de Eucalyptus globulus sobre o crescimento e desenvolvimento de Penicillium sp.
title In vitro effect of Eucalyptus globulus essential oil on Penicillium sp.
spellingShingle In vitro effect of Eucalyptus globulus essential oil on Penicillium sp.
Piatti, Andréia
Fungus
Citrus
Moulds
Eucalyptus
Post-harvest control.
Fungo
Citros
Podridões
Eucalipto
Controle pós-colheita.
title_short In vitro effect of Eucalyptus globulus essential oil on Penicillium sp.
title_full In vitro effect of Eucalyptus globulus essential oil on Penicillium sp.
title_fullStr In vitro effect of Eucalyptus globulus essential oil on Penicillium sp.
title_full_unstemmed In vitro effect of Eucalyptus globulus essential oil on Penicillium sp.
title_sort In vitro effect of Eucalyptus globulus essential oil on Penicillium sp.
author Piatti, Andréia
author_facet Piatti, Andréia
Schneider, Cristina Fernanda
Nozaki, Marcia Holanda
author_role author
author2 Schneider, Cristina Fernanda
Nozaki, Marcia Holanda
author2_role author
author
dc.contributor.author.fl_str_mv Piatti, Andréia
Schneider, Cristina Fernanda
Nozaki, Marcia Holanda
dc.subject.por.fl_str_mv Fungus
Citrus
Moulds
Eucalyptus
Post-harvest control.
Fungo
Citros
Podridões
Eucalipto
Controle pós-colheita.
topic Fungus
Citrus
Moulds
Eucalyptus
Post-harvest control.
Fungo
Citros
Podridões
Eucalipto
Controle pós-colheita.
description Penicillium sp. is the casual agent of moulds, considered the main citrus post-harvest disease, causing loss on the quality and quantity of marketable fruits. Besides economic loss, it can be included costs, transportation and fruit storage problems. The present work had the objective to verify the fungitoxic action of Eucalyptus globules essential oil on the in vitro control of Penicillium sp., evaluating mycelial growth, production and spores germination. The experiment was conduced on the Microbiology laboratory of PUC, Toledo campus, with eight treatments: 1%, 0,5%, 0,25%, 0,1%, 0,05% e 0,025% concentrations of eucalyptus oil added to PDA media (potato-dextrose-agar), negative control (PDA with addition of 40mg of azoxystrobin/L) and positive control (PDA media only). The experimental design was according to a completely randomized design, with five replicates each treatment. The eucalyptus essential oil inhibited significantly the growth in the 1%, 0,5% e 0,25% concentrations, not differing statistically from control with fungicide azoxystrobin. The treatments presented the same behavior for spores production. For spores germination, the 1% e 0,5% concentrations had better control than treatment with azoxystrobin and the concentration of 0,25%. Others oil concentrations did not present significative results on the evaluations, being statistically equal to positive control (PDA).
publishDate 2011
dc.date.none.fl_str_mv 2011-08-25
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10.5433/1679-0359.2011v32n3p1033
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1679-0359.2011v32n3p1033
identifier_str_mv 10.5433/1679-0359.2011v32n3p1033
dc.language.iso.fl_str_mv por
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dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1679-0359.2011v32n3p1033/8377
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dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 32 No. 3 (2011); 1033-1040
Semina: Ciências Agrárias; v. 32 n. 3 (2011); 1033-1040
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
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reponame_str Semina. Ciências Agrárias (Online)
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