In vitro effect of Eucalyptus globulus essential oil on Penicillium sp.
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1679-0359.2011v32n3p1033 |
Resumo: | Penicillium sp. is the casual agent of moulds, considered the main citrus post-harvest disease, causing loss on the quality and quantity of marketable fruits. Besides economic loss, it can be included costs, transportation and fruit storage problems. The present work had the objective to verify the fungitoxic action of Eucalyptus globules essential oil on the in vitro control of Penicillium sp., evaluating mycelial growth, production and spores germination. The experiment was conduced on the Microbiology laboratory of PUC, Toledo campus, with eight treatments: 1%, 0,5%, 0,25%, 0,1%, 0,05% e 0,025% concentrations of eucalyptus oil added to PDA media (potato-dextrose-agar), negative control (PDA with addition of 40mg of azoxystrobin/L) and positive control (PDA media only). The experimental design was according to a completely randomized design, with five replicates each treatment. The eucalyptus essential oil inhibited significantly the growth in the 1%, 0,5% e 0,25% concentrations, not differing statistically from control with fungicide azoxystrobin. The treatments presented the same behavior for spores production. For spores germination, the 1% e 0,5% concentrations had better control than treatment with azoxystrobin and the concentration of 0,25%. Others oil concentrations did not present significative results on the evaluations, being statistically equal to positive control (PDA). |
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Semina. Ciências Agrárias (Online) |
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In vitro effect of Eucalyptus globulus essential oil on Penicillium sp.Efeito in vitro do óleo essencial de Eucalyptus globulus sobre o crescimento e desenvolvimento de Penicillium sp.FungusCitrusMouldsEucalyptusPost-harvest control.FungoCitrosPodridõesEucaliptoControle pós-colheita.Penicillium sp. is the casual agent of moulds, considered the main citrus post-harvest disease, causing loss on the quality and quantity of marketable fruits. Besides economic loss, it can be included costs, transportation and fruit storage problems. The present work had the objective to verify the fungitoxic action of Eucalyptus globules essential oil on the in vitro control of Penicillium sp., evaluating mycelial growth, production and spores germination. The experiment was conduced on the Microbiology laboratory of PUC, Toledo campus, with eight treatments: 1%, 0,5%, 0,25%, 0,1%, 0,05% e 0,025% concentrations of eucalyptus oil added to PDA media (potato-dextrose-agar), negative control (PDA with addition of 40mg of azoxystrobin/L) and positive control (PDA media only). The experimental design was according to a completely randomized design, with five replicates each treatment. The eucalyptus essential oil inhibited significantly the growth in the 1%, 0,5% e 0,25% concentrations, not differing statistically from control with fungicide azoxystrobin. The treatments presented the same behavior for spores production. For spores germination, the 1% e 0,5% concentrations had better control than treatment with azoxystrobin and the concentration of 0,25%. Others oil concentrations did not present significative results on the evaluations, being statistically equal to positive control (PDA).O fungo Penicillium sp. é o agente causal dos bolores, considerada a principal doença pós-colheita em citros, levando à perdas na qualidade e quantidade dos frutos comercializáveis. Além de perdas econômicas, uma vez que nas perdas pós-colheitas estão inclusos os custos, transporte e armazenagem de frutos. O presente trabalho teve por objetivo verificar a ação fungitóxica do óleo essencial de Eucalyptus globulus no controle in vitro de Penicillium sp., avaliando seu crescimento micelial, produção e a germinação de esporos. O experimento foi conduzido no laboratório de Microbiologia da PUC, campus Toledo, e constituiu-se de oito tratamentos, sendo: uso do óleo essencial de eucalipto nas concentrações de 1%, 0,5%, 0,25%, 0,1%, 0,05% e 0,025% adicionados ao meio de cultura BDA (Batata Dextrose-Ágar) autoclavado, testemunha negativa (meio de cultura com adição de 40 mg de ingrediente ativo azoxystrobin/L do fungicida) e testemunha positiva (apenas meio de cultura). O delineamento experimental utilizado foi inteiramente casualizado, com cinco repetições em cada tratamento. O óleo essencial de eucalipto inibiu o crescimento de forma significativa nas concentrações de 1%, 0,5% e 0,25%, não diferindo estatisticamente do controle com o fungicida azoxystrobin. Os tratamentos apresentaram o mesmo comportamento em relação a produção de esporos. Já na germinação de esporos, as concentrações de 1% e 0,5% obtiveram controle superior ao tratamento com azoxystrobin e a concentração de 0,25%. As demais concentrações de óleo não obtiveram resultados significativos nas avaliações, igualando-se estatisticamente a testemunha positiva (BDA).UEL2011-08-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1679-0359.2011v32n3p103310.5433/1679-0359.2011v32n3p1033Semina: Ciências Agrárias; Vol. 32 No. 3 (2011); 1033-1040Semina: Ciências Agrárias; v. 32 n. 3 (2011); 1033-10401679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1679-0359.2011v32n3p1033/8377Piatti, AndréiaSchneider, Cristina FernandaNozaki, Marcia Holandainfo:eu-repo/semantics/openAccess2015-11-19T18:37:50Zoai:ojs.pkp.sfu.ca:article/3119Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2015-11-19T18:37:50Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
In vitro effect of Eucalyptus globulus essential oil on Penicillium sp. Efeito in vitro do óleo essencial de Eucalyptus globulus sobre o crescimento e desenvolvimento de Penicillium sp. |
title |
In vitro effect of Eucalyptus globulus essential oil on Penicillium sp. |
spellingShingle |
In vitro effect of Eucalyptus globulus essential oil on Penicillium sp. Piatti, Andréia Fungus Citrus Moulds Eucalyptus Post-harvest control. Fungo Citros Podridões Eucalipto Controle pós-colheita. |
title_short |
In vitro effect of Eucalyptus globulus essential oil on Penicillium sp. |
title_full |
In vitro effect of Eucalyptus globulus essential oil on Penicillium sp. |
title_fullStr |
In vitro effect of Eucalyptus globulus essential oil on Penicillium sp. |
title_full_unstemmed |
In vitro effect of Eucalyptus globulus essential oil on Penicillium sp. |
title_sort |
In vitro effect of Eucalyptus globulus essential oil on Penicillium sp. |
author |
Piatti, Andréia |
author_facet |
Piatti, Andréia Schneider, Cristina Fernanda Nozaki, Marcia Holanda |
author_role |
author |
author2 |
Schneider, Cristina Fernanda Nozaki, Marcia Holanda |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Piatti, Andréia Schneider, Cristina Fernanda Nozaki, Marcia Holanda |
dc.subject.por.fl_str_mv |
Fungus Citrus Moulds Eucalyptus Post-harvest control. Fungo Citros Podridões Eucalipto Controle pós-colheita. |
topic |
Fungus Citrus Moulds Eucalyptus Post-harvest control. Fungo Citros Podridões Eucalipto Controle pós-colheita. |
description |
Penicillium sp. is the casual agent of moulds, considered the main citrus post-harvest disease, causing loss on the quality and quantity of marketable fruits. Besides economic loss, it can be included costs, transportation and fruit storage problems. The present work had the objective to verify the fungitoxic action of Eucalyptus globules essential oil on the in vitro control of Penicillium sp., evaluating mycelial growth, production and spores germination. The experiment was conduced on the Microbiology laboratory of PUC, Toledo campus, with eight treatments: 1%, 0,5%, 0,25%, 0,1%, 0,05% e 0,025% concentrations of eucalyptus oil added to PDA media (potato-dextrose-agar), negative control (PDA with addition of 40mg of azoxystrobin/L) and positive control (PDA media only). The experimental design was according to a completely randomized design, with five replicates each treatment. The eucalyptus essential oil inhibited significantly the growth in the 1%, 0,5% e 0,25% concentrations, not differing statistically from control with fungicide azoxystrobin. The treatments presented the same behavior for spores production. For spores germination, the 1% e 0,5% concentrations had better control than treatment with azoxystrobin and the concentration of 0,25%. Others oil concentrations did not present significative results on the evaluations, being statistically equal to positive control (PDA). |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-08-25 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1679-0359.2011v32n3p1033 10.5433/1679-0359.2011v32n3p1033 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1679-0359.2011v32n3p1033 |
identifier_str_mv |
10.5433/1679-0359.2011v32n3p1033 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1679-0359.2011v32n3p1033/8377 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 32 No. 3 (2011); 1033-1040 Semina: Ciências Agrárias; v. 32 n. 3 (2011); 1033-1040 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
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1799306055394525184 |