Investigation of Trichomonas gallinae in passerines

Detalhes bibliográficos
Autor(a) principal: Martínez, Jenny Paola Hidalgo
Data de Publicação: 2023
Outros Autores: Mello, Alexia Brauner de, Giacometi, Marjorie de, Cunha, Rodrigo Casquero, Oliveira, Camila Belmonte, Cleff, Marlete Brum, França, Raqueli Teresinha
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/45994
Resumo: Trichomoniasis, caused by the protozoan Trichomonas gallinae, has as main hosts birds of the Columbidae family, which have a high prevalence of the protozoan without manifesting the disease. The continuous growth of the pigeon population and its cosmopolitan nature mean that today there is a worldwide distribution of this species, being responsible for the distribution and maintenance of the prevalence of trichomoniasis in almost the entire world. The transmission of the disease may be by direct contact, or indirect, through food or water. This indirect route is the reason why such a wide range of bird orders can be infected, very different from columbids such as falconiformes, strigiformes, passerines, piciformes, psittaciformes, gruiformes, galliformes or anseriformes. Thus, the objective of this study was to evaluate the presence of T. gallinae in passerines received at a Wild Animal Screening Center. In order to carry out this study, 300 birds of the order Passerine corresponding to 23 different species were analyzed, received at the Wild Fauna Rehabilitation Center (NURFS) of the Federal University of Pelotas (UFPel), in different seasons of the year between the months of March to October 2021. Samples of swabs from the oropharynx were collected from all individuals, the material was immediately placed in a falcon tube containing Trypticase-Yeast Extract-Maltose (TYM) culture medium and sent to the Laboratory of Protozoology and Entomology (LAPEn), for incubation in a bacteriological growth greenhouse and subsequent identification of the protozoan on a slide in wet mounting under an optical microscope in a 40X objective. Wet mounting on a slide was performed in triplicate and analyzed in its entirety. Of the 300 birds evaluated in in vitro culture for T. gallinae, 25 had inconclusive results and were submitted to PCR analysis, being negative for T. gallinae. Although no positive Passeriformes was found, the monitoring of the occurrence of this protozoan must continue, as it is known that it may easily cause a possible epidemic, leading to losses for the wild fauna that has endangered birds.
id UEL-11_55c20e06f47836f3e536b03ec5b518c1
oai_identifier_str oai:ojs.pkp.sfu.ca:article/45994
network_acronym_str UEL-11
network_name_str Semina. Ciências Agrárias (Online)
repository_id_str
spelling Investigation of Trichomonas gallinae in passerinesInvestigação de Trichomonas gallinae em passeriformesParasitesProtozoaWild birds. Aves silvestresParasitasProtozoários. Trichomoniasis, caused by the protozoan Trichomonas gallinae, has as main hosts birds of the Columbidae family, which have a high prevalence of the protozoan without manifesting the disease. The continuous growth of the pigeon population and its cosmopolitan nature mean that today there is a worldwide distribution of this species, being responsible for the distribution and maintenance of the prevalence of trichomoniasis in almost the entire world. The transmission of the disease may be by direct contact, or indirect, through food or water. This indirect route is the reason why such a wide range of bird orders can be infected, very different from columbids such as falconiformes, strigiformes, passerines, piciformes, psittaciformes, gruiformes, galliformes or anseriformes. Thus, the objective of this study was to evaluate the presence of T. gallinae in passerines received at a Wild Animal Screening Center. In order to carry out this study, 300 birds of the order Passerine corresponding to 23 different species were analyzed, received at the Wild Fauna Rehabilitation Center (NURFS) of the Federal University of Pelotas (UFPel), in different seasons of the year between the months of March to October 2021. Samples of swabs from the oropharynx were collected from all individuals, the material was immediately placed in a falcon tube containing Trypticase-Yeast Extract-Maltose (TYM) culture medium and sent to the Laboratory of Protozoology and Entomology (LAPEn), for incubation in a bacteriological growth greenhouse and subsequent identification of the protozoan on a slide in wet mounting under an optical microscope in a 40X objective. Wet mounting on a slide was performed in triplicate and analyzed in its entirety. Of the 300 birds evaluated in in vitro culture for T. gallinae, 25 had inconclusive results and were submitted to PCR analysis, being negative for T. gallinae. Although no positive Passeriformes was found, the monitoring of the occurrence of this protozoan must continue, as it is known that it may easily cause a possible epidemic, leading to losses for the wild fauna that has endangered birds.Tricomoníase, causada pelo protozoário Trichomonas gallinae, tem como principais hospedeiros aves da família Columbidae, que apresentam alta prevalência do protozoário sem manifestar a doença. O contínuo crescimento da população de pombos e sua natureza cosmopolita fazem com que hoje exista uma distribuição mundial desta espécie, sendo responsável pela distribuição e manutenção da prevalência da tricomoníase em quase todo o mundo. A transmissão da doença pode ser por contato direto, ou indireto, por meio de alimentos ou água. Essa rota indireta é a razão pela qual uma gama tão ampla de ordens de aves pode ser infectada, muito diferente dos columbídeos, como falconiformes, strigiformes, passeriformes, piciformes, psittaciformes, gruiformes, galliformes ou anseriformes. Desta forma, o objetivo desse estudo foi avaliar a presença de T. gallinae em passeriformes recebidos em um Centro de Triagem de Animais Silvestres. Para a realização deste estudo foram analisados 300 aves por conveniência da ordem Passeriforme correpondente a 23 espécies diferentes recebidos no Núcleo de Reabilitação da Fauna Silvestres (NURFS) da Universidade Federal de Pelotas (UFPel), em estações distintas do ano comprendidas entre os meses de março a outubro de 2021. De todos os indivíduos foram colhidas amostra de suabe da orofaringe, o material foi imediatamente acondicionado em tubo falcon contendo meio de cultura Tripticase-Yeast Extract-Maltose (TYM) e encaminhado ao Laboratório de Protozoologia e Entomologia (LAPEn), para incubação em estufa de crecimento bacteriologico e posterior identificação em lamina em montagem úmida no microscópio óptico em objetivo de 40X do protozoário. A montagem úmida em lamina, foi feita em triplicata e analisada em sua totalidade. Das 300 aves avaliadas no cultivo in vitro para T. gallinae, 25 tiveram resultado inconclusivo e foram sumetidas a análise de PCR, sendo negativas para T. gallinae. Embora não tenha sido encontrado nenhum Passeriformes positivo, o monitoramento da ocorrência desse protozoário deve continuar, pois sabe-se que ele pode causar uma possível epidemia facilmente, levando a perdas para a fauna silvestre que possuí aves ameaçadas de extinção.UEL2023-02-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4599410.5433/1679-0359.2023v44n1p113Semina: Ciências Agrárias; Vol. 44 No. 1 (2023); 113-122Semina: Ciências Agrárias; v. 44 n. 1 (2023); 113-1221679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/45994/48507Copyright (c) 2023 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessMartínez, Jenny Paola Hidalgo Mello, Alexia Brauner deGiacometi, Marjorie deCunha, Rodrigo CasqueroOliveira, Camila Belmonte Cleff, Marlete BrumFrança, Raqueli Teresinha2023-09-28T18:31:32Zoai:ojs.pkp.sfu.ca:article/45994Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2023-09-28T18:31:32Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Investigation of Trichomonas gallinae in passerines
Investigação de Trichomonas gallinae em passeriformes
title Investigation of Trichomonas gallinae in passerines
spellingShingle Investigation of Trichomonas gallinae in passerines
Martínez, Jenny Paola Hidalgo
Parasites
Protozoa
Wild birds.
Aves silvestres
Parasitas
Protozoários.
title_short Investigation of Trichomonas gallinae in passerines
title_full Investigation of Trichomonas gallinae in passerines
title_fullStr Investigation of Trichomonas gallinae in passerines
title_full_unstemmed Investigation of Trichomonas gallinae in passerines
title_sort Investigation of Trichomonas gallinae in passerines
author Martínez, Jenny Paola Hidalgo
author_facet Martínez, Jenny Paola Hidalgo
Mello, Alexia Brauner de
Giacometi, Marjorie de
Cunha, Rodrigo Casquero
Oliveira, Camila Belmonte
Cleff, Marlete Brum
França, Raqueli Teresinha
author_role author
author2 Mello, Alexia Brauner de
Giacometi, Marjorie de
Cunha, Rodrigo Casquero
Oliveira, Camila Belmonte
Cleff, Marlete Brum
França, Raqueli Teresinha
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Martínez, Jenny Paola Hidalgo
Mello, Alexia Brauner de
Giacometi, Marjorie de
Cunha, Rodrigo Casquero
Oliveira, Camila Belmonte
Cleff, Marlete Brum
França, Raqueli Teresinha
dc.subject.por.fl_str_mv Parasites
Protozoa
Wild birds.
Aves silvestres
Parasitas
Protozoários.
topic Parasites
Protozoa
Wild birds.
Aves silvestres
Parasitas
Protozoários.
description Trichomoniasis, caused by the protozoan Trichomonas gallinae, has as main hosts birds of the Columbidae family, which have a high prevalence of the protozoan without manifesting the disease. The continuous growth of the pigeon population and its cosmopolitan nature mean that today there is a worldwide distribution of this species, being responsible for the distribution and maintenance of the prevalence of trichomoniasis in almost the entire world. The transmission of the disease may be by direct contact, or indirect, through food or water. This indirect route is the reason why such a wide range of bird orders can be infected, very different from columbids such as falconiformes, strigiformes, passerines, piciformes, psittaciformes, gruiformes, galliformes or anseriformes. Thus, the objective of this study was to evaluate the presence of T. gallinae in passerines received at a Wild Animal Screening Center. In order to carry out this study, 300 birds of the order Passerine corresponding to 23 different species were analyzed, received at the Wild Fauna Rehabilitation Center (NURFS) of the Federal University of Pelotas (UFPel), in different seasons of the year between the months of March to October 2021. Samples of swabs from the oropharynx were collected from all individuals, the material was immediately placed in a falcon tube containing Trypticase-Yeast Extract-Maltose (TYM) culture medium and sent to the Laboratory of Protozoology and Entomology (LAPEn), for incubation in a bacteriological growth greenhouse and subsequent identification of the protozoan on a slide in wet mounting under an optical microscope in a 40X objective. Wet mounting on a slide was performed in triplicate and analyzed in its entirety. Of the 300 birds evaluated in in vitro culture for T. gallinae, 25 had inconclusive results and were submitted to PCR analysis, being negative for T. gallinae. Although no positive Passeriformes was found, the monitoring of the occurrence of this protozoan must continue, as it is known that it may easily cause a possible epidemic, leading to losses for the wild fauna that has endangered birds.
publishDate 2023
dc.date.none.fl_str_mv 2023-02-23
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/45994
10.5433/1679-0359.2023v44n1p113
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/45994
identifier_str_mv 10.5433/1679-0359.2023v44n1p113
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/45994/48507
dc.rights.driver.fl_str_mv Copyright (c) 2023 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2023 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 44 No. 1 (2023); 113-122
Semina: Ciências Agrárias; v. 44 n. 1 (2023); 113-122
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
_version_ 1799306086231048192