Performance of the ELISA test for the diagnosis of cysticercosis using experimentally and naturally cattle infected with metacestode of Taenia saginata
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/16483 |
Resumo: | The bovine taeniasis-cysticercosis complex can be defined as a set of pathological changes caused by the adult form of Taenia saginata in humans and their larval form in animals (Cysticercus bovis). Data on the occurrence of bovine cysticercosis comes from the records of veterinary inspection of meat in slaughterhouses under fiscalization, where some positive cases may go unnoticed, especially in moderate infections. So, it is relevant the use of serological tests that have greater sensitivity than the post-mortem routine exams. Studies have shown the possible application of the ELISA test as a tool for epidemiological studies of the parasitosis and in the identification of animals with cysts. The aim of this study is to determine the detection threshold of the indirect ELISA test, using experimentally and naturally infected animals in detecting cases of cysticercosis. The sensitivity of the test to naturally infected animals using a cut-off 1 and 2 added with 2 standart-deviation (SD) was of 12% and 24.4%, respectively. However, when using the cut-off of 1 and 2 added with 3 SD, the test sensitivity dropped, represented by 14.4% and 1.99% of the test sensitivity. As for the samples from experimentally infected animals, using the cut-off 1 and 2 added with 2 SD the sensitivity was 55.9% and 92.5%; and adding 3 SD the values found were 31.2% and 86%, respectively. The specificity of the test in all situations tested was 100%. It is important to take into account the right choice of control sera used in the ELISA test, whereas, according to its application, it is necessary to increase the sensitivity or specificity. |
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Semina. Ciências Agrárias (Online) |
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Performance of the ELISA test for the diagnosis of cysticercosis using experimentally and naturally cattle infected with metacestode of Taenia saginataDesempenho do ELISA no diagnóstico da cisticercose utilizando bovinos experimentalmente e naturalmente infectados com o metacestódeo de Taenia saginataTaenia saginataELISABovine cysticercosisCut-off.Taenia saginataELISACisticercose bovinaPonto de corte.Inspeção de Produtos de Origem AnimalThe bovine taeniasis-cysticercosis complex can be defined as a set of pathological changes caused by the adult form of Taenia saginata in humans and their larval form in animals (Cysticercus bovis). Data on the occurrence of bovine cysticercosis comes from the records of veterinary inspection of meat in slaughterhouses under fiscalization, where some positive cases may go unnoticed, especially in moderate infections. So, it is relevant the use of serological tests that have greater sensitivity than the post-mortem routine exams. Studies have shown the possible application of the ELISA test as a tool for epidemiological studies of the parasitosis and in the identification of animals with cysts. The aim of this study is to determine the detection threshold of the indirect ELISA test, using experimentally and naturally infected animals in detecting cases of cysticercosis. The sensitivity of the test to naturally infected animals using a cut-off 1 and 2 added with 2 standart-deviation (SD) was of 12% and 24.4%, respectively. However, when using the cut-off of 1 and 2 added with 3 SD, the test sensitivity dropped, represented by 14.4% and 1.99% of the test sensitivity. As for the samples from experimentally infected animals, using the cut-off 1 and 2 added with 2 SD the sensitivity was 55.9% and 92.5%; and adding 3 SD the values found were 31.2% and 86%, respectively. The specificity of the test in all situations tested was 100%. It is important to take into account the right choice of control sera used in the ELISA test, whereas, according to its application, it is necessary to increase the sensitivity or specificity. O complexo teníase-cisticercose bovina pode ser definido como um conjunto de alterações patológicas causadas pela forma adulta da Taenia saginata no humano e por sua forma larvar nos bovinos (Cysticercus bovis). Dados sobre a ocorrência da cisticercose bovina provêm dos registros de inspeção veterinária de carnes em matadouros-frigoríficos inspecionados, onde alguns casos positivos podem passar despercebidos, principalmente em infecções moderadas, tornando-se relevante a utilização de testes sorológicos com maior sensibilidade que o exame post-mortem de rotina. Estudos demonstram a possível aplicação do teste ELISA como ferramenta para estudos epidemiológicos da parasitose e na identificação dos animais portadores de cistos. O objetivo do presente trabalho foi avaliar o desempenho e determinar o limiar de detecção de um ELISA indireto, utilizando animais experimentalmente e naturalmente infectados na detecção de casos de cisticercose. A sensibilidade do teste para animais naturalmente infectados utilizando o ponto de corte 1 e 2 adicionados de 2 SD foi de 12% e 24,4% respectivamente. Contudo quanto utilizado o ponto de corte 1 e 2 adicionados de 3 SD a sensibilidade do teste teve uma queda, representado 1,99% e 14,4%. Já para as amostras de animais experimentalmente infectados utilizando o ponto de corte 1 e 2 adicionados de 2 SD, a sensibilidade ficou em 55,9% e 92,5% e adicionando 3 SD foram encontrados os valores de 31,2% e 86% respectivamente. A especificidade do teste em todas as situações testadas ficou em 100%. É importante levar em consideração a escolha certa dos soros-controle utilizados no teste ELISA, visto que de acordo com sua aplicação torna-se necessário elevar a sensibilidade ou especificidade do teste. UEL2015-04-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisaapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1648310.5433/1679-0359.2015v36n2p807Semina: Ciências Agrárias; Vol. 36 No. 2 (2015); 807-816Semina: Ciências Agrárias; v. 36 n. 2 (2015); 807-8161679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/16483/pdf_631Copyright (c) 2015 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessGuimarães-Peixoto, Rafaella Paola Meneguete dosPinto, Paulo Sérgio de ArrudaNero, Luís AugustoSantos, Tatiane de OliveiraSilva, Letícia Ferreira daAcevedo-Nieto, Emílio CamposRivetti Júnior, Anselmo Vasconcelos2023-01-13T13:59:14Zoai:ojs.pkp.sfu.ca:article/16483Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2023-01-13T13:59:14Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Performance of the ELISA test for the diagnosis of cysticercosis using experimentally and naturally cattle infected with metacestode of Taenia saginata Desempenho do ELISA no diagnóstico da cisticercose utilizando bovinos experimentalmente e naturalmente infectados com o metacestódeo de Taenia saginata |
title |
Performance of the ELISA test for the diagnosis of cysticercosis using experimentally and naturally cattle infected with metacestode of Taenia saginata |
spellingShingle |
Performance of the ELISA test for the diagnosis of cysticercosis using experimentally and naturally cattle infected with metacestode of Taenia saginata Guimarães-Peixoto, Rafaella Paola Meneguete dos Taenia saginata ELISA Bovine cysticercosis Cut-off. Taenia saginata ELISA Cisticercose bovina Ponto de corte. Inspeção de Produtos de Origem Animal |
title_short |
Performance of the ELISA test for the diagnosis of cysticercosis using experimentally and naturally cattle infected with metacestode of Taenia saginata |
title_full |
Performance of the ELISA test for the diagnosis of cysticercosis using experimentally and naturally cattle infected with metacestode of Taenia saginata |
title_fullStr |
Performance of the ELISA test for the diagnosis of cysticercosis using experimentally and naturally cattle infected with metacestode of Taenia saginata |
title_full_unstemmed |
Performance of the ELISA test for the diagnosis of cysticercosis using experimentally and naturally cattle infected with metacestode of Taenia saginata |
title_sort |
Performance of the ELISA test for the diagnosis of cysticercosis using experimentally and naturally cattle infected with metacestode of Taenia saginata |
author |
Guimarães-Peixoto, Rafaella Paola Meneguete dos |
author_facet |
Guimarães-Peixoto, Rafaella Paola Meneguete dos Pinto, Paulo Sérgio de Arruda Nero, Luís Augusto Santos, Tatiane de Oliveira Silva, Letícia Ferreira da Acevedo-Nieto, Emílio Campos Rivetti Júnior, Anselmo Vasconcelos |
author_role |
author |
author2 |
Pinto, Paulo Sérgio de Arruda Nero, Luís Augusto Santos, Tatiane de Oliveira Silva, Letícia Ferreira da Acevedo-Nieto, Emílio Campos Rivetti Júnior, Anselmo Vasconcelos |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Guimarães-Peixoto, Rafaella Paola Meneguete dos Pinto, Paulo Sérgio de Arruda Nero, Luís Augusto Santos, Tatiane de Oliveira Silva, Letícia Ferreira da Acevedo-Nieto, Emílio Campos Rivetti Júnior, Anselmo Vasconcelos |
dc.subject.por.fl_str_mv |
Taenia saginata ELISA Bovine cysticercosis Cut-off. Taenia saginata ELISA Cisticercose bovina Ponto de corte. Inspeção de Produtos de Origem Animal |
topic |
Taenia saginata ELISA Bovine cysticercosis Cut-off. Taenia saginata ELISA Cisticercose bovina Ponto de corte. Inspeção de Produtos de Origem Animal |
description |
The bovine taeniasis-cysticercosis complex can be defined as a set of pathological changes caused by the adult form of Taenia saginata in humans and their larval form in animals (Cysticercus bovis). Data on the occurrence of bovine cysticercosis comes from the records of veterinary inspection of meat in slaughterhouses under fiscalization, where some positive cases may go unnoticed, especially in moderate infections. So, it is relevant the use of serological tests that have greater sensitivity than the post-mortem routine exams. Studies have shown the possible application of the ELISA test as a tool for epidemiological studies of the parasitosis and in the identification of animals with cysts. The aim of this study is to determine the detection threshold of the indirect ELISA test, using experimentally and naturally infected animals in detecting cases of cysticercosis. The sensitivity of the test to naturally infected animals using a cut-off 1 and 2 added with 2 standart-deviation (SD) was of 12% and 24.4%, respectively. However, when using the cut-off of 1 and 2 added with 3 SD, the test sensitivity dropped, represented by 14.4% and 1.99% of the test sensitivity. As for the samples from experimentally infected animals, using the cut-off 1 and 2 added with 2 SD the sensitivity was 55.9% and 92.5%; and adding 3 SD the values found were 31.2% and 86%, respectively. The specificity of the test in all situations tested was 100%. It is important to take into account the right choice of control sera used in the ELISA test, whereas, according to its application, it is necessary to increase the sensitivity or specificity. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-04-22 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pesquisa |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/16483 10.5433/1679-0359.2015v36n2p807 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/16483 |
identifier_str_mv |
10.5433/1679-0359.2015v36n2p807 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/16483/pdf_631 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2015 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2015 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 36 No. 2 (2015); 807-816 Semina: Ciências Agrárias; v. 36 n. 2 (2015); 807-816 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306070916595712 |