Anti-Neospora caninum IgG in fetal bovine serum

Detalhes bibliográficos
Autor(a) principal: Reginato, Caroline Zamperete
Data de Publicação: 2020
Outros Autores: Fernandes, Fagner D'ambroso, Braünig, Patricia, Sangioni, Luís Antônio, Vogel, Fernanda Silveira Flores
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/37627
Resumo: Neospora caninum is an obligate intracellular protozoan, recognized as an important cause of abortion in infection cattle worldwide, and is responsible for major economic losses in beef and dairy herds. There are scarce data on the presence of antibodies in fetal serum samples from beef cattle. The pathogen is straggling mainly by vertical transmission in ruminants. This study aimed to determine the frequency of anti-N. caninum antibodies in fetal bovine serum. Overall, 751 fetal serum samples from slaughterhouses in Uruguaiana and Santo Ângelo (Rio Grande do Sul state) were used. Serologic diagnosis was made using indirect immunofluorescence assay (IFA). Slides were prepared by culturing infected Vero cells using tachyzoites of the N. caninum NC-1 strain and a serum dilution of 1:25. Of the 751 samples analyzed, 11.7% (88/751) were positive in the IFA assay. The results indicate an occurrence of neosporosis in the studied region, as well as the possibility of transplacental transmission of the protozoon, characterizing an important mechanism of propagation for the disease.
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spelling Anti-Neospora caninum IgG in fetal bovine serumAnti-Neospora caninum IgG em soro fetal bovinoSerumAbortionTransplacental transmission.SoroAbortoTransmissão vertical.Neospora caninum is an obligate intracellular protozoan, recognized as an important cause of abortion in infection cattle worldwide, and is responsible for major economic losses in beef and dairy herds. There are scarce data on the presence of antibodies in fetal serum samples from beef cattle. The pathogen is straggling mainly by vertical transmission in ruminants. This study aimed to determine the frequency of anti-N. caninum antibodies in fetal bovine serum. Overall, 751 fetal serum samples from slaughterhouses in Uruguaiana and Santo Ângelo (Rio Grande do Sul state) were used. Serologic diagnosis was made using indirect immunofluorescence assay (IFA). Slides were prepared by culturing infected Vero cells using tachyzoites of the N. caninum NC-1 strain and a serum dilution of 1:25. Of the 751 samples analyzed, 11.7% (88/751) were positive in the IFA assay. The results indicate an occurrence of neosporosis in the studied region, as well as the possibility of transplacental transmission of the protozoon, characterizing an important mechanism of propagation for the disease.O Neospora caninum é um protozoário intracelular obrigatório, reconhecido como uma causa importante de aborto no mundo todo, e é responsável pelas maiores perdas da economia de carne e leite. Há poucos dados da presença de anticorpos em amostras de soro fetal de bovinos de corte. Os patógenos são transmitidos principalmente por transmissão vertical em ruminantes. Este estudo pode ser usado para determinar a frequência de anti-N. caninum em soro fetal bovino. Ao todo, foram utilizadas 751 amostras de soro fetal bovino de matadouros de Uruguaiana e Santo Ângelo (RS). Os diagnósticos sorológicos foram realizados utilizando ensaios de imunufluorescência indireta (IFA). As lâminas foram preparadas por cultura de células Vero infectadas utilizando taquizoítos da estirpe N. caninum NC-1 e uma diluição de soro de 1:25. Das 751 amostras analisadas, 11,7% (88/751) foram positivas para o IFA. Os resultados indicam uma ocorrência de neosporose na região estudada, bem como a possibilidade de transmissão transplacentária do protozoário, caracterizando um importante mecanismo de propagação da doença.UEL2020-06-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3762710.5433/1679-0359.2020v41n5p1809Semina: Ciências Agrárias; Vol. 41 No. 5 (2020); 1809-1812Semina: Ciências Agrárias; v. 41 n. 5 (2020); 1809-18121679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/37627/27542Copyright (c) 2020 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessReginato, Caroline ZampereteFernandes, Fagner D'ambrosoBraünig, PatriciaSangioni, Luís AntônioVogel, Fernanda Silveira Flores2022-10-07T16:07:58Zoai:ojs.pkp.sfu.ca:article/37627Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-07T16:07:58Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Anti-Neospora caninum IgG in fetal bovine serum
Anti-Neospora caninum IgG em soro fetal bovino
title Anti-Neospora caninum IgG in fetal bovine serum
spellingShingle Anti-Neospora caninum IgG in fetal bovine serum
Reginato, Caroline Zamperete
Serum
Abortion
Transplacental transmission.
Soro
Aborto
Transmissão vertical.
title_short Anti-Neospora caninum IgG in fetal bovine serum
title_full Anti-Neospora caninum IgG in fetal bovine serum
title_fullStr Anti-Neospora caninum IgG in fetal bovine serum
title_full_unstemmed Anti-Neospora caninum IgG in fetal bovine serum
title_sort Anti-Neospora caninum IgG in fetal bovine serum
author Reginato, Caroline Zamperete
author_facet Reginato, Caroline Zamperete
Fernandes, Fagner D'ambroso
Braünig, Patricia
Sangioni, Luís Antônio
Vogel, Fernanda Silveira Flores
author_role author
author2 Fernandes, Fagner D'ambroso
Braünig, Patricia
Sangioni, Luís Antônio
Vogel, Fernanda Silveira Flores
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Reginato, Caroline Zamperete
Fernandes, Fagner D'ambroso
Braünig, Patricia
Sangioni, Luís Antônio
Vogel, Fernanda Silveira Flores
dc.subject.por.fl_str_mv Serum
Abortion
Transplacental transmission.
Soro
Aborto
Transmissão vertical.
topic Serum
Abortion
Transplacental transmission.
Soro
Aborto
Transmissão vertical.
description Neospora caninum is an obligate intracellular protozoan, recognized as an important cause of abortion in infection cattle worldwide, and is responsible for major economic losses in beef and dairy herds. There are scarce data on the presence of antibodies in fetal serum samples from beef cattle. The pathogen is straggling mainly by vertical transmission in ruminants. This study aimed to determine the frequency of anti-N. caninum antibodies in fetal bovine serum. Overall, 751 fetal serum samples from slaughterhouses in Uruguaiana and Santo Ângelo (Rio Grande do Sul state) were used. Serologic diagnosis was made using indirect immunofluorescence assay (IFA). Slides were prepared by culturing infected Vero cells using tachyzoites of the N. caninum NC-1 strain and a serum dilution of 1:25. Of the 751 samples analyzed, 11.7% (88/751) were positive in the IFA assay. The results indicate an occurrence of neosporosis in the studied region, as well as the possibility of transplacental transmission of the protozoon, characterizing an important mechanism of propagation for the disease.
publishDate 2020
dc.date.none.fl_str_mv 2020-06-17
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado por pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/37627
10.5433/1679-0359.2020v41n5p1809
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/37627
identifier_str_mv 10.5433/1679-0359.2020v41n5p1809
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/37627/27542
dc.rights.driver.fl_str_mv Copyright (c) 2020 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 41 No. 5 (2020); 1809-1812
Semina: Ciências Agrárias; v. 41 n. 5 (2020); 1809-1812
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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