Occurrence of Cryptosporidium spp. in wild animals living in the Cascavel city park, Paraná, Brazil
Autor(a) principal: | |
---|---|
Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20917 |
Resumo: | Digestive and respiratory tracts parasite’s protozoan, Cryptosporidium spp. now-a-days is a major zoonotic agent, it causes self-limiting diarrhea, remaining in the body passively until the moment immune system decreases, leading to an increase in its multiplication in the mucosa and appearance of clinical signs. As there are few studies on cryptosporidiosis in wild free-living and captive animals, especially in Brazil, this study aimed to identify the presence of Cryptosporidium spp. in feces of captive animals in Cascavel, PR Municipal Zoo. Between 2011 and 2012 there have been four collections of bird feces and five mammalian feces totaling 65 and 118 samples respectively. Samples were sent to the laboratory of Veterinary Parasitology of Federal University of Parana. The feces were diluted, centrifuged and the pellets were used to make blades which were stained by the Ziehl-Neelsen modified method and observed under a microscope with 1000X magnification. Then the blades containing the oocysts were observed under a capture microscope, where they were measured. Mammals showed 49.15% and birds 44.61% of positivity. Oocysts’ sizes ranged from 3,54?m to 5,81?m with an average of 4,32?m for birds and 3,11?m to 5,60?m averaging 4,63?m to mammals. As of yet, there isn’t effective treatment against this parasite and considering that it’s a zoonotic disease, preventive measures should be taken to prevent transmission to humans. |
id |
UEL-11_6111cb1c1ebebcb520490ea8046f86d2 |
---|---|
oai_identifier_str |
oai:ojs.pkp.sfu.ca:article/20917 |
network_acronym_str |
UEL-11 |
network_name_str |
Semina. Ciências Agrárias (Online) |
repository_id_str |
|
spelling |
Occurrence of Cryptosporidium spp. in wild animals living in the Cascavel city park, Paraná, BrazilOcorrência de Cryptosporidium spp. em animais silvestres do Parque municipal de Cascavel, Paraná, BrasilCryptosporidiumWild animalsZiehl-NeelsenZoonosis.CryptosporidiumAnimais silvestresZiehl-NeelsenZoonoses.Digestive and respiratory tracts parasite’s protozoan, Cryptosporidium spp. now-a-days is a major zoonotic agent, it causes self-limiting diarrhea, remaining in the body passively until the moment immune system decreases, leading to an increase in its multiplication in the mucosa and appearance of clinical signs. As there are few studies on cryptosporidiosis in wild free-living and captive animals, especially in Brazil, this study aimed to identify the presence of Cryptosporidium spp. in feces of captive animals in Cascavel, PR Municipal Zoo. Between 2011 and 2012 there have been four collections of bird feces and five mammalian feces totaling 65 and 118 samples respectively. Samples were sent to the laboratory of Veterinary Parasitology of Federal University of Parana. The feces were diluted, centrifuged and the pellets were used to make blades which were stained by the Ziehl-Neelsen modified method and observed under a microscope with 1000X magnification. Then the blades containing the oocysts were observed under a capture microscope, where they were measured. Mammals showed 49.15% and birds 44.61% of positivity. Oocysts’ sizes ranged from 3,54?m to 5,81?m with an average of 4,32?m for birds and 3,11?m to 5,60?m averaging 4,63?m to mammals. As of yet, there isn’t effective treatment against this parasite and considering that it’s a zoonotic disease, preventive measures should be taken to prevent transmission to humans.Protozoário que parasita o trato digestório e respiratório dos animais, o Cryptosporidium spp. é um importante agente zoonótico da atualidade, responsável por causar diarreia auto limitante, permanecendo no organismo de forma passiva até o momento em que há uma queda da imunidade, levando a um aumento na sua multiplicação na mucosa e aparecimento de sinais clínicos. Como há poucos estudos sobre a criptosporidiose em animais silvestres de vida livre e de cativeiro, especialmente no Brasil, esse trabalho teve como objetivo realizar uma pesquisa para identificar a presença de oocistos de Cryptosporidium spp. em fezes dos animais cativos no Zoológico Municipal de Cascavel, Paraná. No período de 2011 e 2012 foram realizadas quatro coletas de fezes de aves e cinco de fezes de mamíferos totalizando 65 e 118 amostras respectivamente. As amostras foram enviadas ao laboratório de parasitologia veterinária da Universidade Federal do Paraná, Setor Palotina. As fezes foram diluídas, centrifugadas e os sedimentos foram utilizados para a confecção de lâminas, que foram coradas pelo método de Ziehl-Neelsen modificado e observadas em microscópio com aumento 1000X. Em seguida as lâminas contendo os oocistos foram observadas em microscópio de captura, onde os mesmos foram mensurados. Os mamíferos apresentaram 49,15% de positividade e as aves 44,61% das amostras positivas. O tamanho dos oocistos variou de 3,54?m a 5,81?m com uma média de 4,32?m para aves e de 3,11?m a 5,60?m com média de 4,63?m para mamíferos. Como ainda não há tratamento eficaz para esse parasito e considerando que se trata de uma zoonose, medidas preventivas devem ser tomadas para evitar a transmissão para humanos.UEL2015-12-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa Empírica de Campoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2091710.5433/1679-0359.2015v36n6Supl2p4323Semina: Ciências Agrárias; Vol. 36 No. 6Supl2 (2015); 4323-4332Semina: Ciências Agrárias; v. 36 n. 6Supl2 (2015); 4323-43321679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20917/17552http://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessSnak, AlessandraGarcia, Felipe GustavoDelgado, Luis Eduardo da SilveiraOsaki, Silvia Cristina2022-12-02T15:05:51Zoai:ojs.pkp.sfu.ca:article/20917Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-12-02T15:05:51Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Occurrence of Cryptosporidium spp. in wild animals living in the Cascavel city park, Paraná, Brazil Ocorrência de Cryptosporidium spp. em animais silvestres do Parque municipal de Cascavel, Paraná, Brasil |
title |
Occurrence of Cryptosporidium spp. in wild animals living in the Cascavel city park, Paraná, Brazil |
spellingShingle |
Occurrence of Cryptosporidium spp. in wild animals living in the Cascavel city park, Paraná, Brazil Snak, Alessandra Cryptosporidium Wild animals Ziehl-Neelsen Zoonosis. Cryptosporidium Animais silvestres Ziehl-Neelsen Zoonoses. |
title_short |
Occurrence of Cryptosporidium spp. in wild animals living in the Cascavel city park, Paraná, Brazil |
title_full |
Occurrence of Cryptosporidium spp. in wild animals living in the Cascavel city park, Paraná, Brazil |
title_fullStr |
Occurrence of Cryptosporidium spp. in wild animals living in the Cascavel city park, Paraná, Brazil |
title_full_unstemmed |
Occurrence of Cryptosporidium spp. in wild animals living in the Cascavel city park, Paraná, Brazil |
title_sort |
Occurrence of Cryptosporidium spp. in wild animals living in the Cascavel city park, Paraná, Brazil |
author |
Snak, Alessandra |
author_facet |
Snak, Alessandra Garcia, Felipe Gustavo Delgado, Luis Eduardo da Silveira Osaki, Silvia Cristina |
author_role |
author |
author2 |
Garcia, Felipe Gustavo Delgado, Luis Eduardo da Silveira Osaki, Silvia Cristina |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Snak, Alessandra Garcia, Felipe Gustavo Delgado, Luis Eduardo da Silveira Osaki, Silvia Cristina |
dc.subject.por.fl_str_mv |
Cryptosporidium Wild animals Ziehl-Neelsen Zoonosis. Cryptosporidium Animais silvestres Ziehl-Neelsen Zoonoses. |
topic |
Cryptosporidium Wild animals Ziehl-Neelsen Zoonosis. Cryptosporidium Animais silvestres Ziehl-Neelsen Zoonoses. |
description |
Digestive and respiratory tracts parasite’s protozoan, Cryptosporidium spp. now-a-days is a major zoonotic agent, it causes self-limiting diarrhea, remaining in the body passively until the moment immune system decreases, leading to an increase in its multiplication in the mucosa and appearance of clinical signs. As there are few studies on cryptosporidiosis in wild free-living and captive animals, especially in Brazil, this study aimed to identify the presence of Cryptosporidium spp. in feces of captive animals in Cascavel, PR Municipal Zoo. Between 2011 and 2012 there have been four collections of bird feces and five mammalian feces totaling 65 and 118 samples respectively. Samples were sent to the laboratory of Veterinary Parasitology of Federal University of Parana. The feces were diluted, centrifuged and the pellets were used to make blades which were stained by the Ziehl-Neelsen modified method and observed under a microscope with 1000X magnification. Then the blades containing the oocysts were observed under a capture microscope, where they were measured. Mammals showed 49.15% and birds 44.61% of positivity. Oocysts’ sizes ranged from 3,54?m to 5,81?m with an average of 4,32?m for birds and 3,11?m to 5,60?m averaging 4,63?m to mammals. As of yet, there isn’t effective treatment against this parasite and considering that it’s a zoonotic disease, preventive measures should be taken to prevent transmission to humans. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-16 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pesquisa Empírica de Campo |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20917 10.5433/1679-0359.2015v36n6Supl2p4323 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20917 |
identifier_str_mv |
10.5433/1679-0359.2015v36n6Supl2p4323 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20917/17552 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 36 No. 6Supl2 (2015); 4323-4332 Semina: Ciências Agrárias; v. 36 n. 6Supl2 (2015); 4323-4332 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306073358729216 |