Occurrence of Cryptosporidium spp. in wild animals living in the Cascavel city park, Paraná, Brazil

Detalhes bibliográficos
Autor(a) principal: Snak, Alessandra
Data de Publicação: 2015
Outros Autores: Garcia, Felipe Gustavo, Delgado, Luis Eduardo da Silveira, Osaki, Silvia Cristina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20917
Resumo: Digestive and respiratory tracts parasite’s protozoan, Cryptosporidium spp. now-a-days is a major zoonotic agent, it causes self-limiting diarrhea, remaining in the body passively until the moment immune system decreases, leading to an increase in its multiplication in the mucosa and appearance of clinical signs. As there are few studies on cryptosporidiosis in wild free-living and captive animals, especially in Brazil, this study aimed to identify the presence of Cryptosporidium spp. in feces of captive animals in Cascavel, PR Municipal Zoo. Between 2011 and 2012 there have been four collections of bird feces and five mammalian feces totaling 65 and 118 samples respectively. Samples were sent to the laboratory of Veterinary Parasitology of Federal University of Parana. The feces were diluted, centrifuged and the pellets were used to make blades which were stained by the Ziehl-Neelsen modified method and observed under a microscope with 1000X magnification. Then the blades containing the oocysts were observed under a capture microscope, where they were measured. Mammals showed 49.15% and birds 44.61% of positivity. Oocysts’ sizes ranged from 3,54?m to 5,81?m with an average of 4,32?m for birds and 3,11?m to 5,60?m averaging 4,63?m to mammals. As of yet, there isn’t effective treatment against this parasite and considering that it’s a zoonotic disease, preventive measures should be taken to prevent transmission to humans.
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spelling Occurrence of Cryptosporidium spp. in wild animals living in the Cascavel city park, Paraná, BrazilOcorrência de Cryptosporidium spp. em animais silvestres do Parque municipal de Cascavel, Paraná, BrasilCryptosporidiumWild animalsZiehl-NeelsenZoonosis.CryptosporidiumAnimais silvestresZiehl-NeelsenZoonoses.Digestive and respiratory tracts parasite’s protozoan, Cryptosporidium spp. now-a-days is a major zoonotic agent, it causes self-limiting diarrhea, remaining in the body passively until the moment immune system decreases, leading to an increase in its multiplication in the mucosa and appearance of clinical signs. As there are few studies on cryptosporidiosis in wild free-living and captive animals, especially in Brazil, this study aimed to identify the presence of Cryptosporidium spp. in feces of captive animals in Cascavel, PR Municipal Zoo. Between 2011 and 2012 there have been four collections of bird feces and five mammalian feces totaling 65 and 118 samples respectively. Samples were sent to the laboratory of Veterinary Parasitology of Federal University of Parana. The feces were diluted, centrifuged and the pellets were used to make blades which were stained by the Ziehl-Neelsen modified method and observed under a microscope with 1000X magnification. Then the blades containing the oocysts were observed under a capture microscope, where they were measured. Mammals showed 49.15% and birds 44.61% of positivity. Oocysts’ sizes ranged from 3,54?m to 5,81?m with an average of 4,32?m for birds and 3,11?m to 5,60?m averaging 4,63?m to mammals. As of yet, there isn’t effective treatment against this parasite and considering that it’s a zoonotic disease, preventive measures should be taken to prevent transmission to humans.Protozoário que parasita o trato digestório e respiratório dos animais, o Cryptosporidium spp. é um importante agente zoonótico da atualidade, responsável por causar diarreia auto limitante, permanecendo no organismo de forma passiva até o momento em que há uma queda da imunidade, levando a um aumento na sua multiplicação na mucosa e aparecimento de sinais clínicos. Como há poucos estudos sobre a criptosporidiose em animais silvestres de vida livre e de cativeiro, especialmente no Brasil, esse trabalho teve como objetivo realizar uma pesquisa para identificar a presença de oocistos de Cryptosporidium spp. em fezes dos animais cativos no Zoológico Municipal de Cascavel, Paraná. No período de 2011 e 2012 foram realizadas quatro coletas de fezes de aves e cinco de fezes de mamíferos totalizando 65 e 118 amostras respectivamente. As amostras foram enviadas ao laboratório de parasitologia veterinária da Universidade Federal do Paraná, Setor Palotina. As fezes foram diluídas, centrifugadas e os sedimentos foram utilizados para a confecção de lâminas, que foram coradas pelo método de Ziehl-Neelsen modificado e observadas em microscópio com aumento 1000X. Em seguida as lâminas contendo os oocistos foram observadas em microscópio de captura, onde os mesmos foram mensurados. Os mamíferos apresentaram 49,15% de positividade e as aves 44,61% das amostras positivas. O tamanho dos oocistos variou de 3,54?m a 5,81?m com uma média de 4,32?m para aves e de 3,11?m a 5,60?m com média de 4,63?m para mamíferos. Como ainda não há tratamento eficaz para esse parasito e considerando que se trata de uma zoonose, medidas preventivas devem ser tomadas para evitar a transmissão para humanos.UEL2015-12-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa Empírica de Campoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2091710.5433/1679-0359.2015v36n6Supl2p4323Semina: Ciências Agrárias; Vol. 36 No. 6Supl2 (2015); 4323-4332Semina: Ciências Agrárias; v. 36 n. 6Supl2 (2015); 4323-43321679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20917/17552http://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessSnak, AlessandraGarcia, Felipe GustavoDelgado, Luis Eduardo da SilveiraOsaki, Silvia Cristina2022-12-02T15:05:51Zoai:ojs.pkp.sfu.ca:article/20917Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-12-02T15:05:51Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Occurrence of Cryptosporidium spp. in wild animals living in the Cascavel city park, Paraná, Brazil
Ocorrência de Cryptosporidium spp. em animais silvestres do Parque municipal de Cascavel, Paraná, Brasil
title Occurrence of Cryptosporidium spp. in wild animals living in the Cascavel city park, Paraná, Brazil
spellingShingle Occurrence of Cryptosporidium spp. in wild animals living in the Cascavel city park, Paraná, Brazil
Snak, Alessandra
Cryptosporidium
Wild animals
Ziehl-Neelsen
Zoonosis.
Cryptosporidium
Animais silvestres
Ziehl-Neelsen
Zoonoses.
title_short Occurrence of Cryptosporidium spp. in wild animals living in the Cascavel city park, Paraná, Brazil
title_full Occurrence of Cryptosporidium spp. in wild animals living in the Cascavel city park, Paraná, Brazil
title_fullStr Occurrence of Cryptosporidium spp. in wild animals living in the Cascavel city park, Paraná, Brazil
title_full_unstemmed Occurrence of Cryptosporidium spp. in wild animals living in the Cascavel city park, Paraná, Brazil
title_sort Occurrence of Cryptosporidium spp. in wild animals living in the Cascavel city park, Paraná, Brazil
author Snak, Alessandra
author_facet Snak, Alessandra
Garcia, Felipe Gustavo
Delgado, Luis Eduardo da Silveira
Osaki, Silvia Cristina
author_role author
author2 Garcia, Felipe Gustavo
Delgado, Luis Eduardo da Silveira
Osaki, Silvia Cristina
author2_role author
author
author
dc.contributor.author.fl_str_mv Snak, Alessandra
Garcia, Felipe Gustavo
Delgado, Luis Eduardo da Silveira
Osaki, Silvia Cristina
dc.subject.por.fl_str_mv Cryptosporidium
Wild animals
Ziehl-Neelsen
Zoonosis.
Cryptosporidium
Animais silvestres
Ziehl-Neelsen
Zoonoses.
topic Cryptosporidium
Wild animals
Ziehl-Neelsen
Zoonosis.
Cryptosporidium
Animais silvestres
Ziehl-Neelsen
Zoonoses.
description Digestive and respiratory tracts parasite’s protozoan, Cryptosporidium spp. now-a-days is a major zoonotic agent, it causes self-limiting diarrhea, remaining in the body passively until the moment immune system decreases, leading to an increase in its multiplication in the mucosa and appearance of clinical signs. As there are few studies on cryptosporidiosis in wild free-living and captive animals, especially in Brazil, this study aimed to identify the presence of Cryptosporidium spp. in feces of captive animals in Cascavel, PR Municipal Zoo. Between 2011 and 2012 there have been four collections of bird feces and five mammalian feces totaling 65 and 118 samples respectively. Samples were sent to the laboratory of Veterinary Parasitology of Federal University of Parana. The feces were diluted, centrifuged and the pellets were used to make blades which were stained by the Ziehl-Neelsen modified method and observed under a microscope with 1000X magnification. Then the blades containing the oocysts were observed under a capture microscope, where they were measured. Mammals showed 49.15% and birds 44.61% of positivity. Oocysts’ sizes ranged from 3,54?m to 5,81?m with an average of 4,32?m for birds and 3,11?m to 5,60?m averaging 4,63?m to mammals. As of yet, there isn’t effective treatment against this parasite and considering that it’s a zoonotic disease, preventive measures should be taken to prevent transmission to humans.
publishDate 2015
dc.date.none.fl_str_mv 2015-12-16
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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format article
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dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20917
10.5433/1679-0359.2015v36n6Supl2p4323
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20917
identifier_str_mv 10.5433/1679-0359.2015v36n6Supl2p4323
dc.language.iso.fl_str_mv eng
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dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20917/17552
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info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 36 No. 6Supl2 (2015); 4323-4332
Semina: Ciências Agrárias; v. 36 n. 6Supl2 (2015); 4323-4332
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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