Investigation of Anaplasma marginale, Babesia bovis, Babesia bigemina and Trypanosoma vivax in the brain and spleen of dairy cows of Rio Grande do Sul

Detalhes bibliográficos
Autor(a) principal: Rigo, Melânia Lazzari
Data de Publicação: 2023
Outros Autores: Martins, Kauê Rodriguez, Islabão, Yan Wahast, Mello, Alexia Brauner de, Giacometi, Marjorie de, Cunha, Rodrigo Casquero, Rovani, Monique Tomazele, Oliveira, Camila Belmonte
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/48510
Resumo: Cattle tick fever and bovine trypanosomosis are diseases that negatively impact cattle production in Brazil, causing considerably productive losses and animals death. In this study, the objective was to investigate the presence of the four hemoparasites that mostly affect dairy herds (A. marginale, B. bovis, B. bigemina and T. vivax) using the qPCR technique in discarded dairy cows in the northwestern region of Rio Grande do Sul. For this purpose, brain and spleen tissue biopsies were collected at a local slaughterhouse from 64 dairy cattle from this region. DNA extraction from the samples was performed using the Brazol® reagent, their quantity and purity were obtained through spectrophotometer analysis with NanoDropTM. In the brain samples, DNA detection was performed for T. vivax and B. bovis, and in the spleen, DNA of the four agents was verified. From a total of 64 brain samples analyzed, 14.06 % were positive for Babesia bovis. For spleen samples, Babesia bovis was detected in 17.19 % of the samples, while 29.69 % tested positive for B. bigemina and 37.50 % for Anaplasma marginale. Regarding Trypanosoma vivax, none of the analyzed samples resulted in a positive diagnosis in both tissues. Thus, the findings highlighted that B. bovis, B. bigemina and A. marginale are present in the dairy cattle herd of the northwestern regions of Rio Grande do Sul, with a higher percentage of A. marginale; on the other hand, T. vivax was not diagnosed in the present study.
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spelling Investigation of Anaplasma marginale, Babesia bovis, Babesia bigemina and Trypanosoma vivax in the brain and spleen of dairy cows of Rio Grande do SulInvestigação de Anaplasma marginale, Babesia bovis, Babesia bigemina e Trypanosoma vivax em cérebro e baço de vacas leiteiras no Rio Grande do SulcattlehemoparasitosisslaughterhouseCattle tick fever and bovine trypanosomosis are diseases that negatively impact cattle production in Brazil, causing considerably productive losses and animals death. In this study, the objective was to investigate the presence of the four hemoparasites that mostly affect dairy herds (A. marginale, B. bovis, B. bigemina and T. vivax) using the qPCR technique in discarded dairy cows in the northwestern region of Rio Grande do Sul. For this purpose, brain and spleen tissue biopsies were collected at a local slaughterhouse from 64 dairy cattle from this region. DNA extraction from the samples was performed using the Brazol® reagent, their quantity and purity were obtained through spectrophotometer analysis with NanoDropTM. In the brain samples, DNA detection was performed for T. vivax and B. bovis, and in the spleen, DNA of the four agents was verified. From a total of 64 brain samples analyzed, 14.06 % were positive for Babesia bovis. For spleen samples, Babesia bovis was detected in 17.19 % of the samples, while 29.69 % tested positive for B. bigemina and 37.50 % for Anaplasma marginale. Regarding Trypanosoma vivax, none of the analyzed samples resulted in a positive diagnosis in both tissues. Thus, the findings highlighted that B. bovis, B. bigemina and A. marginale are present in the dairy cattle herd of the northwestern regions of Rio Grande do Sul, with a higher percentage of A. marginale; on the other hand, T. vivax was not diagnosed in the present study.A Tristeza Parasitária Bovina (TPB) e a tripanossomose bovina são enfermidades que impactam a produção bovina de forma negativa no Brasil, causando perdas na produção e morte dos animais. Neste estudo, o objetivo foi investigar os quatro hemoparasitos que mais acometem o rebanho bovino (A. marginale, B. bovis, B. bigemina e T. vivax) pela técnica de qPCR, em vacas leiteiras de descarte na região noroeste do Rio Grande do Sul. Para isso, foram coletados, em abatedouro, fragmentos de cérebro e de baço de 64 bovinos leiteiros dessa região. A extração do DNA das amostras foi feita por meio do reagente Brazol®, e a quantidade e a pureza das mesmas, foi obtida através da análise de espectrofotômetro com NanoDropTM. Nas amostras de cérebro foi realizada a detecção de DNA para T. vivax e B. bovis, enquanto que no baço, investigou-se DNA dos quatro agentes. Das 64 amostras de cérebro analisadas, 14,06 % foram positivas para Babesia bovis. Nas amostras de baço, Babesia bovis foi detectada em 17,19 %, enquanto que 29,69 % testaram positivas para B. bigemina e, 37,50 %, para Anaplasma marginale. Em relação ao Trypanosoma vivax, nenhuma resultou positiva em ambos os tecidos. Assim, os achados evidenciaram que B. bovis, B. bigemina e A. marginale estão presentes no rebanho bovino leiteiro, tendo maior percentual de A. marginale na região investigada; por outro lado, T. vivax não foi diagnosticado no presente estudo.UEL2023-12-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4851010.5433/1679-0359.2023v44n6p2063Semina: Ciências Agrárias; Vol. 44 No. 6 (2023); 2063-2078Semina: Ciências Agrárias; v. 44 n. 6 (2023); 2063-20781679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/48510/49794Copyright (c) 2023 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessRigo, Melânia LazzariMartins, Kauê RodriguezIslabão, Yan WahastMello, Alexia Brauner deGiacometi, Marjorie deCunha, Rodrigo CasqueroRovani, Monique TomazeleOliveira, Camila Belmonte2024-01-30T13:16:11Zoai:ojs.pkp.sfu.ca:article/48510Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2024-01-30T13:16:11Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Investigation of Anaplasma marginale, Babesia bovis, Babesia bigemina and Trypanosoma vivax in the brain and spleen of dairy cows of Rio Grande do Sul
Investigação de Anaplasma marginale, Babesia bovis, Babesia bigemina e Trypanosoma vivax em cérebro e baço de vacas leiteiras no Rio Grande do Sul
title Investigation of Anaplasma marginale, Babesia bovis, Babesia bigemina and Trypanosoma vivax in the brain and spleen of dairy cows of Rio Grande do Sul
spellingShingle Investigation of Anaplasma marginale, Babesia bovis, Babesia bigemina and Trypanosoma vivax in the brain and spleen of dairy cows of Rio Grande do Sul
Rigo, Melânia Lazzari
cattle
hemoparasitosis
slaughterhouse
title_short Investigation of Anaplasma marginale, Babesia bovis, Babesia bigemina and Trypanosoma vivax in the brain and spleen of dairy cows of Rio Grande do Sul
title_full Investigation of Anaplasma marginale, Babesia bovis, Babesia bigemina and Trypanosoma vivax in the brain and spleen of dairy cows of Rio Grande do Sul
title_fullStr Investigation of Anaplasma marginale, Babesia bovis, Babesia bigemina and Trypanosoma vivax in the brain and spleen of dairy cows of Rio Grande do Sul
title_full_unstemmed Investigation of Anaplasma marginale, Babesia bovis, Babesia bigemina and Trypanosoma vivax in the brain and spleen of dairy cows of Rio Grande do Sul
title_sort Investigation of Anaplasma marginale, Babesia bovis, Babesia bigemina and Trypanosoma vivax in the brain and spleen of dairy cows of Rio Grande do Sul
author Rigo, Melânia Lazzari
author_facet Rigo, Melânia Lazzari
Martins, Kauê Rodriguez
Islabão, Yan Wahast
Mello, Alexia Brauner de
Giacometi, Marjorie de
Cunha, Rodrigo Casquero
Rovani, Monique Tomazele
Oliveira, Camila Belmonte
author_role author
author2 Martins, Kauê Rodriguez
Islabão, Yan Wahast
Mello, Alexia Brauner de
Giacometi, Marjorie de
Cunha, Rodrigo Casquero
Rovani, Monique Tomazele
Oliveira, Camila Belmonte
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rigo, Melânia Lazzari
Martins, Kauê Rodriguez
Islabão, Yan Wahast
Mello, Alexia Brauner de
Giacometi, Marjorie de
Cunha, Rodrigo Casquero
Rovani, Monique Tomazele
Oliveira, Camila Belmonte
dc.subject.por.fl_str_mv cattle
hemoparasitosis
slaughterhouse
topic cattle
hemoparasitosis
slaughterhouse
description Cattle tick fever and bovine trypanosomosis are diseases that negatively impact cattle production in Brazil, causing considerably productive losses and animals death. In this study, the objective was to investigate the presence of the four hemoparasites that mostly affect dairy herds (A. marginale, B. bovis, B. bigemina and T. vivax) using the qPCR technique in discarded dairy cows in the northwestern region of Rio Grande do Sul. For this purpose, brain and spleen tissue biopsies were collected at a local slaughterhouse from 64 dairy cattle from this region. DNA extraction from the samples was performed using the Brazol® reagent, their quantity and purity were obtained through spectrophotometer analysis with NanoDropTM. In the brain samples, DNA detection was performed for T. vivax and B. bovis, and in the spleen, DNA of the four agents was verified. From a total of 64 brain samples analyzed, 14.06 % were positive for Babesia bovis. For spleen samples, Babesia bovis was detected in 17.19 % of the samples, while 29.69 % tested positive for B. bigemina and 37.50 % for Anaplasma marginale. Regarding Trypanosoma vivax, none of the analyzed samples resulted in a positive diagnosis in both tissues. Thus, the findings highlighted that B. bovis, B. bigemina and A. marginale are present in the dairy cattle herd of the northwestern regions of Rio Grande do Sul, with a higher percentage of A. marginale; on the other hand, T. vivax was not diagnosed in the present study.
publishDate 2023
dc.date.none.fl_str_mv 2023-12-18
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/48510
10.5433/1679-0359.2023v44n6p2063
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/48510
identifier_str_mv 10.5433/1679-0359.2023v44n6p2063
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/48510/49794
dc.rights.driver.fl_str_mv Copyright (c) 2023 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2023 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 44 No. 6 (2023); 2063-2078
Semina: Ciências Agrárias; v. 44 n. 6 (2023); 2063-2078
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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