Dynamics of Rickettsia parkeri infection in domestic chickens
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/21352 |
Resumo: | The aim of the present study was to investigate experimental infections by Rickettsia parkeri in domestic chickens (Gallus gallus domesticus) and to determine the dynamics of antibody production during acute and chronic infection. The animals (n = 64) were allocated into eight groups as follows: G1, inoculated intramuscularly (IM) with 2.5 × 105 Vero cells (1 mL) infected with R. parkeri; G2, inoculated IM with 5.0 × 105 Vero cells (2 mL) infected with R. parkeri; G3, received 1 mL of the inoculum subcutaneously (SC); G4, received 2 mL of inoculum SC; G5, received 1 mL of the inoculum intraperitoneally (IP); G6, injected with 2 mL of the inoculum IP; G7 and G8, received 1 mL and 2 mL of culture medium IM, respectively (negative control groups). All R. parkeri inocula were viable prior to inoculation in the birds. In order to assess the dynamics of antibody production in acute and chronic infection, sera of chickens were collected 3, 7, 14, and 21 d post infection (PI) and assessed using an immunofluorescence antibody test (IFAT). In addition, PCR (gltA gene) was performed using fragments of spleen and lung from euthanized chickens to detect the replication of R. parkeri in tissues during the experimental period. Animals from the G4 and G3 groups exhibited the highest mean antibody titers, with maximum levels observed at 7 and 14 d PI, respectively. Conversely, G2, G4 and G6 exhibited higher mean antibody titers than G1, G3 and G5, respectively. Antibody titers were dose-dependent. Rickettsial DNA was not detected in either spleen or lung tissue. The present study demonstrated that birds seroconvert after being challenged by R. parkeri. However, there was no replication of the agent in the tissues analyzed and rickettsemia was not observed. |
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Semina. Ciências Agrárias (Online) |
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Dynamics of Rickettsia parkeri infection in domestic chickensDinâmica da infecção por Rickettsia parkeri em galinhas domésticasGallus gallus domesticusImmunofluorescence antibody testInoculationPolymerase chain reactionSpotted fever.Febre maculosaGallus gallus domesticusInoculaçãoReação de imunofluorescência indiretaReação em cadeia da polimerase.The aim of the present study was to investigate experimental infections by Rickettsia parkeri in domestic chickens (Gallus gallus domesticus) and to determine the dynamics of antibody production during acute and chronic infection. The animals (n = 64) were allocated into eight groups as follows: G1, inoculated intramuscularly (IM) with 2.5 × 105 Vero cells (1 mL) infected with R. parkeri; G2, inoculated IM with 5.0 × 105 Vero cells (2 mL) infected with R. parkeri; G3, received 1 mL of the inoculum subcutaneously (SC); G4, received 2 mL of inoculum SC; G5, received 1 mL of the inoculum intraperitoneally (IP); G6, injected with 2 mL of the inoculum IP; G7 and G8, received 1 mL and 2 mL of culture medium IM, respectively (negative control groups). All R. parkeri inocula were viable prior to inoculation in the birds. In order to assess the dynamics of antibody production in acute and chronic infection, sera of chickens were collected 3, 7, 14, and 21 d post infection (PI) and assessed using an immunofluorescence antibody test (IFAT). In addition, PCR (gltA gene) was performed using fragments of spleen and lung from euthanized chickens to detect the replication of R. parkeri in tissues during the experimental period. Animals from the G4 and G3 groups exhibited the highest mean antibody titers, with maximum levels observed at 7 and 14 d PI, respectively. Conversely, G2, G4 and G6 exhibited higher mean antibody titers than G1, G3 and G5, respectively. Antibody titers were dose-dependent. Rickettsial DNA was not detected in either spleen or lung tissue. The present study demonstrated that birds seroconvert after being challenged by R. parkeri. However, there was no replication of the agent in the tissues analyzed and rickettsemia was not observed.O objetivo deste estudo foi avaliar a infecção experimental por Rickettsia parkeri em galinhas domésticas (Gallus gallus domesticus) e investigar a dinâmica de anticorpos. Para tanto, foram utilizadas 64 galinhas criadas extensivamente divididas em oito grupos: G1 - inoculado com 2,5 x 105 células Vero (1 ml) infectadas por R. parkeri via intramuscular (IM); G2 – 5,0 x 105 células Vero (2 ml) infectadas por R. parkeri via IM; G3 - 1 ml do inóculo via subcutânea (SC); G4 - 2 ml de inóculo via SC; G5 – 1 ml do inóculo via intraperitoneal (IP); G6 - 2 ml de inóculo via IP, e G7 e G8 - 1 e 2 ml de meio de cultivo de células Vero via IM, respectivamente representando os grupos controles negativos. Ressaltase que todos os inóculos de R. parkeri estavam viáveis no momento da inoculação. Os soros das aves foram coletados aos 3, 7, 14 e 21 dias pós-infecção (PI) e testadas por reação de imunofluorescência indireta (RIFI) para avaliar a dinâmica de anticorpos na infecção aguda e crônica. A identificação da multiplicação de R. parkeri nos tecidos, no mesmo período PI, foi realizada por PCR, para um fragmento do gene gltA, em amostras de baço e pulmão provenientes das galinhas eutanasiadas. As aves do G4 e G3 apresentaram as maiores médias de anticorpos obtendo os níveis mais elevados aos sete e 14 dias PI, respectivamente. G2, G4 e G6 apresentaram médias de anticorpos superiores comparados aos G1, G3 e G5 respectivamente, sendo considerada a infecção dose-dependente. Não foi detectado DNA rickettsial nos tecidos avaliados. No presente trabalho foi possível demonstrar que as aves soroconverteram mediante o desafio da inoculação por R. parkeri, todavia não houve a identificação do agente nos tecidos analisados, bem como a presença de rickettsemia.UEL2016-02-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa CientíficaPesquisa Científicaapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2135210.5433/1679-0359.2016v37n1p233Semina: Ciências Agrárias; Vol. 37 No. 1 (2016); 233-242Semina: Ciências Agrárias; v. 37 n. 1 (2016); 233-2421679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/21352/17994http://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessMaciel, Jonas FernandesBrustolin, Joice MagaliKrawczak, Felipe da SilvaAlves, Marta Elena MachadoPivoto, Felipe LambertiMoraes-Filho, JonasLabruna, Marcelo BahiaLovato, MaristelaVogel, Fernanda Silveira FloresBotton, Sônia de AvilaSangioni, Luis Antônio2022-12-02T13:23:51Zoai:ojs.pkp.sfu.ca:article/21352Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-12-02T13:23:51Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Dynamics of Rickettsia parkeri infection in domestic chickens Dinâmica da infecção por Rickettsia parkeri em galinhas domésticas |
title |
Dynamics of Rickettsia parkeri infection in domestic chickens |
spellingShingle |
Dynamics of Rickettsia parkeri infection in domestic chickens Maciel, Jonas Fernandes Gallus gallus domesticus Immunofluorescence antibody test Inoculation Polymerase chain reaction Spotted fever. Febre maculosa Gallus gallus domesticus Inoculação Reação de imunofluorescência indireta Reação em cadeia da polimerase. |
title_short |
Dynamics of Rickettsia parkeri infection in domestic chickens |
title_full |
Dynamics of Rickettsia parkeri infection in domestic chickens |
title_fullStr |
Dynamics of Rickettsia parkeri infection in domestic chickens |
title_full_unstemmed |
Dynamics of Rickettsia parkeri infection in domestic chickens |
title_sort |
Dynamics of Rickettsia parkeri infection in domestic chickens |
author |
Maciel, Jonas Fernandes |
author_facet |
Maciel, Jonas Fernandes Brustolin, Joice Magali Krawczak, Felipe da Silva Alves, Marta Elena Machado Pivoto, Felipe Lamberti Moraes-Filho, Jonas Labruna, Marcelo Bahia Lovato, Maristela Vogel, Fernanda Silveira Flores Botton, Sônia de Avila Sangioni, Luis Antônio |
author_role |
author |
author2 |
Brustolin, Joice Magali Krawczak, Felipe da Silva Alves, Marta Elena Machado Pivoto, Felipe Lamberti Moraes-Filho, Jonas Labruna, Marcelo Bahia Lovato, Maristela Vogel, Fernanda Silveira Flores Botton, Sônia de Avila Sangioni, Luis Antônio |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Maciel, Jonas Fernandes Brustolin, Joice Magali Krawczak, Felipe da Silva Alves, Marta Elena Machado Pivoto, Felipe Lamberti Moraes-Filho, Jonas Labruna, Marcelo Bahia Lovato, Maristela Vogel, Fernanda Silveira Flores Botton, Sônia de Avila Sangioni, Luis Antônio |
dc.subject.por.fl_str_mv |
Gallus gallus domesticus Immunofluorescence antibody test Inoculation Polymerase chain reaction Spotted fever. Febre maculosa Gallus gallus domesticus Inoculação Reação de imunofluorescência indireta Reação em cadeia da polimerase. |
topic |
Gallus gallus domesticus Immunofluorescence antibody test Inoculation Polymerase chain reaction Spotted fever. Febre maculosa Gallus gallus domesticus Inoculação Reação de imunofluorescência indireta Reação em cadeia da polimerase. |
description |
The aim of the present study was to investigate experimental infections by Rickettsia parkeri in domestic chickens (Gallus gallus domesticus) and to determine the dynamics of antibody production during acute and chronic infection. The animals (n = 64) were allocated into eight groups as follows: G1, inoculated intramuscularly (IM) with 2.5 × 105 Vero cells (1 mL) infected with R. parkeri; G2, inoculated IM with 5.0 × 105 Vero cells (2 mL) infected with R. parkeri; G3, received 1 mL of the inoculum subcutaneously (SC); G4, received 2 mL of inoculum SC; G5, received 1 mL of the inoculum intraperitoneally (IP); G6, injected with 2 mL of the inoculum IP; G7 and G8, received 1 mL and 2 mL of culture medium IM, respectively (negative control groups). All R. parkeri inocula were viable prior to inoculation in the birds. In order to assess the dynamics of antibody production in acute and chronic infection, sera of chickens were collected 3, 7, 14, and 21 d post infection (PI) and assessed using an immunofluorescence antibody test (IFAT). In addition, PCR (gltA gene) was performed using fragments of spleen and lung from euthanized chickens to detect the replication of R. parkeri in tissues during the experimental period. Animals from the G4 and G3 groups exhibited the highest mean antibody titers, with maximum levels observed at 7 and 14 d PI, respectively. Conversely, G2, G4 and G6 exhibited higher mean antibody titers than G1, G3 and G5, respectively. Antibody titers were dose-dependent. Rickettsial DNA was not detected in either spleen or lung tissue. The present study demonstrated that birds seroconvert after being challenged by R. parkeri. However, there was no replication of the agent in the tissues analyzed and rickettsemia was not observed. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-02-29 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pesquisa Científica Pesquisa Científica |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/21352 10.5433/1679-0359.2016v37n1p233 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/21352 |
identifier_str_mv |
10.5433/1679-0359.2016v37n1p233 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/21352/17994 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 37 No. 1 (2016); 233-242 Semina: Ciências Agrárias; v. 37 n. 1 (2016); 233-242 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306073865191424 |