Prevalence and risk factors for Leptospira spp. in dairy cattle in western Paraná, Brazil

Detalhes bibliográficos
Autor(a) principal: Candeias, Ana Paula Molinari
Data de Publicação: 2023
Outros Autores: Snak, Alessandra, Pretto-Giordano, Lucienne Garcia, Osaki, Silvia Cristina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/46067
Resumo: Leptospirosis is caused by spirochete bacteria of the genus Leptospira and is considered the most widespread zoonosis worldwide. It is an important agent that causes animal production to decrease. In cattle, it affects especially the reproductive tract. The objective of this study was to determine the seroprevalence of Leptospira spp., molecularly detect the bacteria in tissues of aborted fetuses, and identify the main risk factors associated with infection in cattle in dairy farms in Western Paraná. For this purpose, 600 bovine serum samples from 60 properties and 17 bovine fetuses from nine properties were collected. Data about the properties were also collected through an epidemiological questionnaire to assess the main risk factors associated with Leptospira spp. infection. The serum samples were analyzed using microscopic agglutination test (MAT), and the fetal tissues using nested polymerase chain reaction (nested PCR). Seroprevalence of Leptospira spp. in dairy cattle in Western Paraná was 39.83% (239/600) and none of the analyzed fetuses were positive for Leptospira spp. The main risk factors identified are related to the production system, reproductive management, and the presence of dogs on the property. Leptospira spp. infection is widely spread in the cattle population in Western Paraná.
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spelling Prevalence and risk factors for Leptospira spp. in dairy cattle in western Paraná, BrazilPrevalência e estudo dos fatores de risco da Leptospira spp. em bovinos leiteiros na região Oeste do Paraná, BrasilSeroprevalence. Protease.LeptospirosisCattleMaintenance host. SoroprevalênciaNested-PCRLeptospiroseBovinosHospedeiro de manutenção.Leptospirosis is caused by spirochete bacteria of the genus Leptospira and is considered the most widespread zoonosis worldwide. It is an important agent that causes animal production to decrease. In cattle, it affects especially the reproductive tract. The objective of this study was to determine the seroprevalence of Leptospira spp., molecularly detect the bacteria in tissues of aborted fetuses, and identify the main risk factors associated with infection in cattle in dairy farms in Western Paraná. For this purpose, 600 bovine serum samples from 60 properties and 17 bovine fetuses from nine properties were collected. Data about the properties were also collected through an epidemiological questionnaire to assess the main risk factors associated with Leptospira spp. infection. The serum samples were analyzed using microscopic agglutination test (MAT), and the fetal tissues using nested polymerase chain reaction (nested PCR). Seroprevalence of Leptospira spp. in dairy cattle in Western Paraná was 39.83% (239/600) and none of the analyzed fetuses were positive for Leptospira spp. The main risk factors identified are related to the production system, reproductive management, and the presence of dogs on the property. Leptospira spp. infection is widely spread in the cattle population in Western Paraná.A leptospirose tem como agente as bactérias espiroquetas do gênero Leptospira e é considerada a zoonose mais difundida pelo mundo. É um importante agente que causa diminuição da produção animal, sendo que nos bovinos ela acomete especialmente o trato reprodutivo. O objetivo desse estudo foi determinar a soroprevalência de Leptospira spp., detectar molecularmente a bactéria em tecidos de fetos abortados e identificar os principais fatores de risco associados à infecção em bovinos provenientes de propriedades leiteiras da região Oeste do Paraná. Para isso, foram coletadas 600 amostras de soro bovino provenientes de 60 propriedades e 17 fetos bovinos de nove propriedades. A partir de um questionário epidemiológico, também foram coletados dados das propriedades para avaliar os principais fatores de risco associados à infecção por Leptospira spp. As amostras de soro foram analisadas pela técnica de soroaglutinação microscópica (SAM) e os tecidos fetais por meio da Dupla Reação em Cadeia pela Polimerase (nested-PCR). A soroprevalência de Leptospira spp. em bovinos leiteiros da região Oeste do Paraná foi de 39,83% (239/600). Nenhum feto analisado apresentou resultado positivo para Leptospira spp. Os principais fatores de risco identificados estão relacionados ao perfil de produção, manejo reprodutivo e a presença de cães na propriedade. A infecção   por Leptospira spp. está amplamente distribuída na população bovina da região Oeste do estado do Paraná.  UEL2023-08-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4606710.5433/1679-0359.2023v44n3p1097Semina: Ciências Agrárias; Vol. 44 No. 3 (2023); 1097-1112Semina: Ciências Agrárias; v. 44 n. 3 (2023); 1097-11121679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/46067/49297Copyright (c) 2023 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessCandeias, Ana Paula MolinariSnak, AlessandraPretto-Giordano, Lucienne GarciaOsaki, Silvia Cristina2023-10-03T16:40:27Zoai:ojs.pkp.sfu.ca:article/46067Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2023-10-03T16:40:27Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Prevalence and risk factors for Leptospira spp. in dairy cattle in western Paraná, Brazil
Prevalência e estudo dos fatores de risco da Leptospira spp. em bovinos leiteiros na região Oeste do Paraná, Brasil
title Prevalence and risk factors for Leptospira spp. in dairy cattle in western Paraná, Brazil
spellingShingle Prevalence and risk factors for Leptospira spp. in dairy cattle in western Paraná, Brazil
Candeias, Ana Paula Molinari
Seroprevalence
. Protease.
Leptospirosis
Cattle
Maintenance host.
Soroprevalência
Nested-PCR
Leptospirose
Bovinos
Hospedeiro de manutenção.
title_short Prevalence and risk factors for Leptospira spp. in dairy cattle in western Paraná, Brazil
title_full Prevalence and risk factors for Leptospira spp. in dairy cattle in western Paraná, Brazil
title_fullStr Prevalence and risk factors for Leptospira spp. in dairy cattle in western Paraná, Brazil
title_full_unstemmed Prevalence and risk factors for Leptospira spp. in dairy cattle in western Paraná, Brazil
title_sort Prevalence and risk factors for Leptospira spp. in dairy cattle in western Paraná, Brazil
author Candeias, Ana Paula Molinari
author_facet Candeias, Ana Paula Molinari
Snak, Alessandra
Pretto-Giordano, Lucienne Garcia
Osaki, Silvia Cristina
author_role author
author2 Snak, Alessandra
Pretto-Giordano, Lucienne Garcia
Osaki, Silvia Cristina
author2_role author
author
author
dc.contributor.author.fl_str_mv Candeias, Ana Paula Molinari
Snak, Alessandra
Pretto-Giordano, Lucienne Garcia
Osaki, Silvia Cristina
dc.subject.por.fl_str_mv Seroprevalence
. Protease.
Leptospirosis
Cattle
Maintenance host.
Soroprevalência
Nested-PCR
Leptospirose
Bovinos
Hospedeiro de manutenção.
topic Seroprevalence
. Protease.
Leptospirosis
Cattle
Maintenance host.
Soroprevalência
Nested-PCR
Leptospirose
Bovinos
Hospedeiro de manutenção.
description Leptospirosis is caused by spirochete bacteria of the genus Leptospira and is considered the most widespread zoonosis worldwide. It is an important agent that causes animal production to decrease. In cattle, it affects especially the reproductive tract. The objective of this study was to determine the seroprevalence of Leptospira spp., molecularly detect the bacteria in tissues of aborted fetuses, and identify the main risk factors associated with infection in cattle in dairy farms in Western Paraná. For this purpose, 600 bovine serum samples from 60 properties and 17 bovine fetuses from nine properties were collected. Data about the properties were also collected through an epidemiological questionnaire to assess the main risk factors associated with Leptospira spp. infection. The serum samples were analyzed using microscopic agglutination test (MAT), and the fetal tissues using nested polymerase chain reaction (nested PCR). Seroprevalence of Leptospira spp. in dairy cattle in Western Paraná was 39.83% (239/600) and none of the analyzed fetuses were positive for Leptospira spp. The main risk factors identified are related to the production system, reproductive management, and the presence of dogs on the property. Leptospira spp. infection is widely spread in the cattle population in Western Paraná.
publishDate 2023
dc.date.none.fl_str_mv 2023-08-22
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/46067
10.5433/1679-0359.2023v44n3p1097
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/46067
identifier_str_mv 10.5433/1679-0359.2023v44n3p1097
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/46067/49297
dc.rights.driver.fl_str_mv Copyright (c) 2023 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2023 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 44 No. 3 (2023); 1097-1112
Semina: Ciências Agrárias; v. 44 n. 3 (2023); 1097-1112
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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