Bioassay on banana rhizome discs for selecting bacteria antagonistic to Fusarium oxysporum f. sp. cubense

Detalhes bibliográficos
Autor(a) principal: Luquine, Liliane Santana
Data de Publicação: 2020
Outros Autores: Pinheiro, Luciano Ricardo Braga, Oliveira, Saulo Alves Santos de, Silva, Harllen Sandro Alves
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36955
Resumo: Detached plant organs are alternative materials to in vitro tests for selecting biocontrol agents. On the other hand, the use of scales to quantify injured areas can generate inconsistent results. Rhizospheric and endophytic bacteria were selected as growth inhibitors of Fusarium oxysporum f. sp. cubense (Foc), the causal agent of Panama disease of banana. For this, rhizome discs were treated with 200 µL of antagonist suspension (109 CFU mL?1) and inoculated with the pathogen. The material was placed in plastic gerbox boxes and incubated in BOD at 25 °C for 12 days. Afterward, the area of rhizome discs (mm2) colonized by Foc was quantified by digital images. The assay was set up in a completely randomized design, with four replications and three discs per replication. The control consisted of untreated and inoculated discs. The results showed the efficiency of this method in selecting the biological control agent, as the 26 isolates were group into five different clusters, with isolates belonging to four of these groups (from ‘1’ to ‘4’) being able to reduce Foc colonization. Isolates 520EB, 993EB, and 531EB had the highest potential for inhibition, with areas of 343.3, 344.1, 364.8 mm2, respectively, promoting inhibition ranging from 53 to 56 % of the colonized area compared to the control (782.6 mm2).
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spelling Bioassay on banana rhizome discs for selecting bacteria antagonistic to Fusarium oxysporum f. sp. cubenseBioensaio em discos de rizomas de bananeiras para a seleção de bactérias antagonistas a Fusarium oxysporum f. sp. cubensePanama diseaseMusa spp.biological control.Mal-do-PanamáMusa sppControle biológico.Detached plant organs are alternative materials to in vitro tests for selecting biocontrol agents. On the other hand, the use of scales to quantify injured areas can generate inconsistent results. Rhizospheric and endophytic bacteria were selected as growth inhibitors of Fusarium oxysporum f. sp. cubense (Foc), the causal agent of Panama disease of banana. For this, rhizome discs were treated with 200 µL of antagonist suspension (109 CFU mL?1) and inoculated with the pathogen. The material was placed in plastic gerbox boxes and incubated in BOD at 25 °C for 12 days. Afterward, the area of rhizome discs (mm2) colonized by Foc was quantified by digital images. The assay was set up in a completely randomized design, with four replications and three discs per replication. The control consisted of untreated and inoculated discs. The results showed the efficiency of this method in selecting the biological control agent, as the 26 isolates were group into five different clusters, with isolates belonging to four of these groups (from ‘1’ to ‘4’) being able to reduce Foc colonization. Isolates 520EB, 993EB, and 531EB had the highest potential for inhibition, with areas of 343.3, 344.1, 364.8 mm2, respectively, promoting inhibition ranging from 53 to 56 % of the colonized area compared to the control (782.6 mm2).O emprego de órgãos vegetais destacados é uma alternativa aos testes in vitro para seleção de agentes de biocontrole. Por outro lado, o uso de escalas para quantificação de área lesionada pode gerar resultados inconsistentes. Bactérias rizosféricas e endofíticas foram selecionadas como inibidoras do crescimento de Fusarium oxysporum f. sp. cubense (Foc), agente causal do mal-do-Panamá da bananeira. Para tanto, discos de rizoma foram tratados com 200 µL de suspensão dos antagonistas (109 UFC mL-1) e inoculados com patógeno. O material foi disposto em caixas plásticas tipo gerbox e incubado em BOD a 25 ºC, por doze dias. Após, a área dos discos de rizoma (mm2) colonizada por Foc dos discos foi quantificada por meio de imagens digitais. O ensaio foi montado em delineamento inteiramente casualizado, com quatro repetições e três discos por repetição. Discos não tratados e inoculados compuseram o controle. Com base nos resultados obtidos, foi possível verificar a eficiência do método na seleção de agente de controle biológico, uma vez que foi possível o agrupamento dos 26 isolados utilizados em cinco clústeres distintos, sendo que isolados pertencentes a quatro destes grupos (do ‘1’ a ‘4’) foram capazes de proporcionar a redução da colonização por Foc. Os isolados com maior potencial de inibição foram 520EB, 993EB e 531EB com 343,3, 344,1, 364,8 mm2, respectivamente, promovendo inibição variando de 53 a 56 % da área colonizada quando comparados com ao controle (782,6 mm2).UEL2020-08-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionMethological researchAvaliado por paresPesquisa metodológicaapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3695510.5433/1679-0359.2020v41n5supl1p2401Semina: Ciências Agrárias; Vol. 41 No. 5supl1 (2020); 2401-2410Semina: Ciências Agrárias; v. 41 n. 5supl1 (2020); 2401-24101679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36955/27814Copyright (c) 2020 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessLuquine, Liliane SantanaPinheiro, Luciano Ricardo BragaOliveira, Saulo Alves Santos deSilva, Harllen Sandro Alves2022-10-07T14:20:55Zoai:ojs.pkp.sfu.ca:article/36955Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-07T14:20:55Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Bioassay on banana rhizome discs for selecting bacteria antagonistic to Fusarium oxysporum f. sp. cubense
Bioensaio em discos de rizomas de bananeiras para a seleção de bactérias antagonistas a Fusarium oxysporum f. sp. cubense
title Bioassay on banana rhizome discs for selecting bacteria antagonistic to Fusarium oxysporum f. sp. cubense
spellingShingle Bioassay on banana rhizome discs for selecting bacteria antagonistic to Fusarium oxysporum f. sp. cubense
Luquine, Liliane Santana
Panama disease
Musa spp.
biological control.
Mal-do-Panamá
Musa spp
Controle biológico.
title_short Bioassay on banana rhizome discs for selecting bacteria antagonistic to Fusarium oxysporum f. sp. cubense
title_full Bioassay on banana rhizome discs for selecting bacteria antagonistic to Fusarium oxysporum f. sp. cubense
title_fullStr Bioassay on banana rhizome discs for selecting bacteria antagonistic to Fusarium oxysporum f. sp. cubense
title_full_unstemmed Bioassay on banana rhizome discs for selecting bacteria antagonistic to Fusarium oxysporum f. sp. cubense
title_sort Bioassay on banana rhizome discs for selecting bacteria antagonistic to Fusarium oxysporum f. sp. cubense
author Luquine, Liliane Santana
author_facet Luquine, Liliane Santana
Pinheiro, Luciano Ricardo Braga
Oliveira, Saulo Alves Santos de
Silva, Harllen Sandro Alves
author_role author
author2 Pinheiro, Luciano Ricardo Braga
Oliveira, Saulo Alves Santos de
Silva, Harllen Sandro Alves
author2_role author
author
author
dc.contributor.author.fl_str_mv Luquine, Liliane Santana
Pinheiro, Luciano Ricardo Braga
Oliveira, Saulo Alves Santos de
Silva, Harllen Sandro Alves
dc.subject.por.fl_str_mv Panama disease
Musa spp.
biological control.
Mal-do-Panamá
Musa spp
Controle biológico.
topic Panama disease
Musa spp.
biological control.
Mal-do-Panamá
Musa spp
Controle biológico.
description Detached plant organs are alternative materials to in vitro tests for selecting biocontrol agents. On the other hand, the use of scales to quantify injured areas can generate inconsistent results. Rhizospheric and endophytic bacteria were selected as growth inhibitors of Fusarium oxysporum f. sp. cubense (Foc), the causal agent of Panama disease of banana. For this, rhizome discs were treated with 200 µL of antagonist suspension (109 CFU mL?1) and inoculated with the pathogen. The material was placed in plastic gerbox boxes and incubated in BOD at 25 °C for 12 days. Afterward, the area of rhizome discs (mm2) colonized by Foc was quantified by digital images. The assay was set up in a completely randomized design, with four replications and three discs per replication. The control consisted of untreated and inoculated discs. The results showed the efficiency of this method in selecting the biological control agent, as the 26 isolates were group into five different clusters, with isolates belonging to four of these groups (from ‘1’ to ‘4’) being able to reduce Foc colonization. Isolates 520EB, 993EB, and 531EB had the highest potential for inhibition, with areas of 343.3, 344.1, 364.8 mm2, respectively, promoting inhibition ranging from 53 to 56 % of the colonized area compared to the control (782.6 mm2).
publishDate 2020
dc.date.none.fl_str_mv 2020-08-07
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Methological research
Avaliado por pares
Pesquisa metodológica
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36955
10.5433/1679-0359.2020v41n5supl1p2401
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36955
identifier_str_mv 10.5433/1679-0359.2020v41n5supl1p2401
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36955/27814
dc.rights.driver.fl_str_mv Copyright (c) 2020 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 41 No. 5supl1 (2020); 2401-2410
Semina: Ciências Agrárias; v. 41 n. 5supl1 (2020); 2401-2410
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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