Bioassay on banana rhizome discs for selecting bacteria antagonistic to Fusarium oxysporum f. sp. cubense
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36955 |
Resumo: | Detached plant organs are alternative materials to in vitro tests for selecting biocontrol agents. On the other hand, the use of scales to quantify injured areas can generate inconsistent results. Rhizospheric and endophytic bacteria were selected as growth inhibitors of Fusarium oxysporum f. sp. cubense (Foc), the causal agent of Panama disease of banana. For this, rhizome discs were treated with 200 µL of antagonist suspension (109 CFU mL?1) and inoculated with the pathogen. The material was placed in plastic gerbox boxes and incubated in BOD at 25 °C for 12 days. Afterward, the area of rhizome discs (mm2) colonized by Foc was quantified by digital images. The assay was set up in a completely randomized design, with four replications and three discs per replication. The control consisted of untreated and inoculated discs. The results showed the efficiency of this method in selecting the biological control agent, as the 26 isolates were group into five different clusters, with isolates belonging to four of these groups (from ‘1’ to ‘4’) being able to reduce Foc colonization. Isolates 520EB, 993EB, and 531EB had the highest potential for inhibition, with areas of 343.3, 344.1, 364.8 mm2, respectively, promoting inhibition ranging from 53 to 56 % of the colonized area compared to the control (782.6 mm2). |
id |
UEL-11_6697b6c2007bd6fbe9bbb6f07ad954f5 |
---|---|
oai_identifier_str |
oai:ojs.pkp.sfu.ca:article/36955 |
network_acronym_str |
UEL-11 |
network_name_str |
Semina. Ciências Agrárias (Online) |
repository_id_str |
|
spelling |
Bioassay on banana rhizome discs for selecting bacteria antagonistic to Fusarium oxysporum f. sp. cubenseBioensaio em discos de rizomas de bananeiras para a seleção de bactérias antagonistas a Fusarium oxysporum f. sp. cubensePanama diseaseMusa spp.biological control.Mal-do-PanamáMusa sppControle biológico.Detached plant organs are alternative materials to in vitro tests for selecting biocontrol agents. On the other hand, the use of scales to quantify injured areas can generate inconsistent results. Rhizospheric and endophytic bacteria were selected as growth inhibitors of Fusarium oxysporum f. sp. cubense (Foc), the causal agent of Panama disease of banana. For this, rhizome discs were treated with 200 µL of antagonist suspension (109 CFU mL?1) and inoculated with the pathogen. The material was placed in plastic gerbox boxes and incubated in BOD at 25 °C for 12 days. Afterward, the area of rhizome discs (mm2) colonized by Foc was quantified by digital images. The assay was set up in a completely randomized design, with four replications and three discs per replication. The control consisted of untreated and inoculated discs. The results showed the efficiency of this method in selecting the biological control agent, as the 26 isolates were group into five different clusters, with isolates belonging to four of these groups (from ‘1’ to ‘4’) being able to reduce Foc colonization. Isolates 520EB, 993EB, and 531EB had the highest potential for inhibition, with areas of 343.3, 344.1, 364.8 mm2, respectively, promoting inhibition ranging from 53 to 56 % of the colonized area compared to the control (782.6 mm2).O emprego de órgãos vegetais destacados é uma alternativa aos testes in vitro para seleção de agentes de biocontrole. Por outro lado, o uso de escalas para quantificação de área lesionada pode gerar resultados inconsistentes. Bactérias rizosféricas e endofíticas foram selecionadas como inibidoras do crescimento de Fusarium oxysporum f. sp. cubense (Foc), agente causal do mal-do-Panamá da bananeira. Para tanto, discos de rizoma foram tratados com 200 µL de suspensão dos antagonistas (109 UFC mL-1) e inoculados com patógeno. O material foi disposto em caixas plásticas tipo gerbox e incubado em BOD a 25 ºC, por doze dias. Após, a área dos discos de rizoma (mm2) colonizada por Foc dos discos foi quantificada por meio de imagens digitais. O ensaio foi montado em delineamento inteiramente casualizado, com quatro repetições e três discos por repetição. Discos não tratados e inoculados compuseram o controle. Com base nos resultados obtidos, foi possível verificar a eficiência do método na seleção de agente de controle biológico, uma vez que foi possível o agrupamento dos 26 isolados utilizados em cinco clústeres distintos, sendo que isolados pertencentes a quatro destes grupos (do ‘1’ a ‘4’) foram capazes de proporcionar a redução da colonização por Foc. Os isolados com maior potencial de inibição foram 520EB, 993EB e 531EB com 343,3, 344,1, 364,8 mm2, respectivamente, promovendo inibição variando de 53 a 56 % da área colonizada quando comparados com ao controle (782,6 mm2).UEL2020-08-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionMethological researchAvaliado por paresPesquisa metodológicaapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3695510.5433/1679-0359.2020v41n5supl1p2401Semina: Ciências Agrárias; Vol. 41 No. 5supl1 (2020); 2401-2410Semina: Ciências Agrárias; v. 41 n. 5supl1 (2020); 2401-24101679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36955/27814Copyright (c) 2020 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessLuquine, Liliane SantanaPinheiro, Luciano Ricardo BragaOliveira, Saulo Alves Santos deSilva, Harllen Sandro Alves2022-10-07T14:20:55Zoai:ojs.pkp.sfu.ca:article/36955Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-07T14:20:55Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Bioassay on banana rhizome discs for selecting bacteria antagonistic to Fusarium oxysporum f. sp. cubense Bioensaio em discos de rizomas de bananeiras para a seleção de bactérias antagonistas a Fusarium oxysporum f. sp. cubense |
title |
Bioassay on banana rhizome discs for selecting bacteria antagonistic to Fusarium oxysporum f. sp. cubense |
spellingShingle |
Bioassay on banana rhizome discs for selecting bacteria antagonistic to Fusarium oxysporum f. sp. cubense Luquine, Liliane Santana Panama disease Musa spp. biological control. Mal-do-Panamá Musa spp Controle biológico. |
title_short |
Bioassay on banana rhizome discs for selecting bacteria antagonistic to Fusarium oxysporum f. sp. cubense |
title_full |
Bioassay on banana rhizome discs for selecting bacteria antagonistic to Fusarium oxysporum f. sp. cubense |
title_fullStr |
Bioassay on banana rhizome discs for selecting bacteria antagonistic to Fusarium oxysporum f. sp. cubense |
title_full_unstemmed |
Bioassay on banana rhizome discs for selecting bacteria antagonistic to Fusarium oxysporum f. sp. cubense |
title_sort |
Bioassay on banana rhizome discs for selecting bacteria antagonistic to Fusarium oxysporum f. sp. cubense |
author |
Luquine, Liliane Santana |
author_facet |
Luquine, Liliane Santana Pinheiro, Luciano Ricardo Braga Oliveira, Saulo Alves Santos de Silva, Harllen Sandro Alves |
author_role |
author |
author2 |
Pinheiro, Luciano Ricardo Braga Oliveira, Saulo Alves Santos de Silva, Harllen Sandro Alves |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Luquine, Liliane Santana Pinheiro, Luciano Ricardo Braga Oliveira, Saulo Alves Santos de Silva, Harllen Sandro Alves |
dc.subject.por.fl_str_mv |
Panama disease Musa spp. biological control. Mal-do-Panamá Musa spp Controle biológico. |
topic |
Panama disease Musa spp. biological control. Mal-do-Panamá Musa spp Controle biológico. |
description |
Detached plant organs are alternative materials to in vitro tests for selecting biocontrol agents. On the other hand, the use of scales to quantify injured areas can generate inconsistent results. Rhizospheric and endophytic bacteria were selected as growth inhibitors of Fusarium oxysporum f. sp. cubense (Foc), the causal agent of Panama disease of banana. For this, rhizome discs were treated with 200 µL of antagonist suspension (109 CFU mL?1) and inoculated with the pathogen. The material was placed in plastic gerbox boxes and incubated in BOD at 25 °C for 12 days. Afterward, the area of rhizome discs (mm2) colonized by Foc was quantified by digital images. The assay was set up in a completely randomized design, with four replications and three discs per replication. The control consisted of untreated and inoculated discs. The results showed the efficiency of this method in selecting the biological control agent, as the 26 isolates were group into five different clusters, with isolates belonging to four of these groups (from ‘1’ to ‘4’) being able to reduce Foc colonization. Isolates 520EB, 993EB, and 531EB had the highest potential for inhibition, with areas of 343.3, 344.1, 364.8 mm2, respectively, promoting inhibition ranging from 53 to 56 % of the colonized area compared to the control (782.6 mm2). |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-08-07 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Methological research Avaliado por pares Pesquisa metodológica |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36955 10.5433/1679-0359.2020v41n5supl1p2401 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36955 |
identifier_str_mv |
10.5433/1679-0359.2020v41n5supl1p2401 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36955/27814 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 41 No. 5supl1 (2020); 2401-2410 Semina: Ciências Agrárias; v. 41 n. 5supl1 (2020); 2401-2410 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306081698054144 |