Characterization of enteropathy in mice infected with Giardia duodenalis and treated with differing anti-parasite drugs

Detalhes bibliográficos
Autor(a) principal: Elias, Marcelo Alberto
Data de Publicação: 2020
Outros Autores: Pavanelli, Mariana Felgueira, Melo, Gessilda de Alcântara Nogueira de, Sant´Ana, Débora de Mello Goncales
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36925
Resumo: Our aim was to analyze the duodena of mice infected with Giardia duodenalis (assemblage A) after four different types of parasite treatment for enteropathy. Methods: Forty-eight male Swiss mice, 21 days of age, received 1000 parasite cysts orally. The animals were divided into six groups: negative (G1) and positive control (G2), and groups treated with nitazoxanide (G3), albendazole (G4), metronidazole (G5), and secnidazole (G6). After treatment they were sacrificed and the duodena were examined histopathologically. Results: In the positive control group G2, increased levels of neutral and acidic goblet cells, intraepithelial lymphocytes, and Paneth cells were found. There was a reduction in villus height and an increase in villus width; and crypts presented increased widths and depths. Hypertrophy in all layers of the intestinal wall and enterocytes was also observed. Conclusions: Metronidazole was the most effective in attenuating the enteropathy caused by G. duodenalis infection; reversing the increase in intraepithelial lymphocytes and Paneth cells, and attenuating the other elements investigated.
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spelling Characterization of enteropathy in mice infected with Giardia duodenalis and treated with differing anti-parasite drugsCaracterização da enteropatia em camundongos infectados com Giardia duodenalis e tratados com diferentes medicamentos antiparasitáriosGiardia sppGiardiasisParasitosisDuodenumAntiparasitic.Giardia sppGiardíaseParasitosesDuodenoAntiparasitários.Our aim was to analyze the duodena of mice infected with Giardia duodenalis (assemblage A) after four different types of parasite treatment for enteropathy. Methods: Forty-eight male Swiss mice, 21 days of age, received 1000 parasite cysts orally. The animals were divided into six groups: negative (G1) and positive control (G2), and groups treated with nitazoxanide (G3), albendazole (G4), metronidazole (G5), and secnidazole (G6). After treatment they were sacrificed and the duodena were examined histopathologically. Results: In the positive control group G2, increased levels of neutral and acidic goblet cells, intraepithelial lymphocytes, and Paneth cells were found. There was a reduction in villus height and an increase in villus width; and crypts presented increased widths and depths. Hypertrophy in all layers of the intestinal wall and enterocytes was also observed. Conclusions: Metronidazole was the most effective in attenuating the enteropathy caused by G. duodenalis infection; reversing the increase in intraepithelial lymphocytes and Paneth cells, and attenuating the other elements investigated.Nosso objetivo foi analisar a enteropatia do duodeno de camundongos infectados com Giardia duodenalis (assembleia A) e submetê-los a quatro tipos diferentes de tratamento parasitário. Métodos: Quarenta e oito camundongos Swiss machos, com 21 dias de idade, receberam 1000 cistos de parasitas por via oral. Os animais foram divididos em seis grupos: controle negativo (G1), controle positivo (G2) e tratamento com nitazoxanida (G3), albendazol (G4), metronidazol (G5) e secnidazol (G6). Eles foram então sacrificados e o duodeno foi examinado histopatologicamente. Resultados: No grupo G2, foram encontrados níveis aumentados de células caliciformes neutras e ácidas, linfócitos intra-epiteliais e células de Paneth. Houve redução da altura das vilosidades e aumento da largura das vilosidades; as criptas aumentaram em largura e profundidade. Hipertrofia em todas as camadas da parede intestinal e enterócitos também foi observada. Conclusões: O metronidazol foi mais efetivo na atenuação da enteropatia causada pela infecção por G. duodenalis, pois reverteu o aumento dos linfócitos intraepiteliais e das células de Paneth e atenuou os demais elementos investigados.UEL2020-06-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionModelo animal, técnicas histológicasapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3692510.5433/1679-0359.2020v41n5p1625Semina: Ciências Agrárias; Vol. 41 No. 5 (2020); 1625-1638Semina: Ciências Agrárias; v. 41 n. 5 (2020); 1625-16381679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36925/27528Copyright (c) 2020 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessElias, Marcelo AlbertoPavanelli, Mariana FelgueiraMelo, Gessilda de Alcântara Nogueira deSant´Ana, Débora de Mello Goncales2022-10-07T15:50:20Zoai:ojs.pkp.sfu.ca:article/36925Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-07T15:50:20Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Characterization of enteropathy in mice infected with Giardia duodenalis and treated with differing anti-parasite drugs
Caracterização da enteropatia em camundongos infectados com Giardia duodenalis e tratados com diferentes medicamentos antiparasitários
title Characterization of enteropathy in mice infected with Giardia duodenalis and treated with differing anti-parasite drugs
spellingShingle Characterization of enteropathy in mice infected with Giardia duodenalis and treated with differing anti-parasite drugs
Elias, Marcelo Alberto
Giardia spp
Giardiasis
Parasitosis
Duodenum
Antiparasitic.
Giardia spp
Giardíase
Parasitoses
Duodeno
Antiparasitários.
title_short Characterization of enteropathy in mice infected with Giardia duodenalis and treated with differing anti-parasite drugs
title_full Characterization of enteropathy in mice infected with Giardia duodenalis and treated with differing anti-parasite drugs
title_fullStr Characterization of enteropathy in mice infected with Giardia duodenalis and treated with differing anti-parasite drugs
title_full_unstemmed Characterization of enteropathy in mice infected with Giardia duodenalis and treated with differing anti-parasite drugs
title_sort Characterization of enteropathy in mice infected with Giardia duodenalis and treated with differing anti-parasite drugs
author Elias, Marcelo Alberto
author_facet Elias, Marcelo Alberto
Pavanelli, Mariana Felgueira
Melo, Gessilda de Alcântara Nogueira de
Sant´Ana, Débora de Mello Goncales
author_role author
author2 Pavanelli, Mariana Felgueira
Melo, Gessilda de Alcântara Nogueira de
Sant´Ana, Débora de Mello Goncales
author2_role author
author
author
dc.contributor.author.fl_str_mv Elias, Marcelo Alberto
Pavanelli, Mariana Felgueira
Melo, Gessilda de Alcântara Nogueira de
Sant´Ana, Débora de Mello Goncales
dc.subject.por.fl_str_mv Giardia spp
Giardiasis
Parasitosis
Duodenum
Antiparasitic.
Giardia spp
Giardíase
Parasitoses
Duodeno
Antiparasitários.
topic Giardia spp
Giardiasis
Parasitosis
Duodenum
Antiparasitic.
Giardia spp
Giardíase
Parasitoses
Duodeno
Antiparasitários.
description Our aim was to analyze the duodena of mice infected with Giardia duodenalis (assemblage A) after four different types of parasite treatment for enteropathy. Methods: Forty-eight male Swiss mice, 21 days of age, received 1000 parasite cysts orally. The animals were divided into six groups: negative (G1) and positive control (G2), and groups treated with nitazoxanide (G3), albendazole (G4), metronidazole (G5), and secnidazole (G6). After treatment they were sacrificed and the duodena were examined histopathologically. Results: In the positive control group G2, increased levels of neutral and acidic goblet cells, intraepithelial lymphocytes, and Paneth cells were found. There was a reduction in villus height and an increase in villus width; and crypts presented increased widths and depths. Hypertrophy in all layers of the intestinal wall and enterocytes was also observed. Conclusions: Metronidazole was the most effective in attenuating the enteropathy caused by G. duodenalis infection; reversing the increase in intraepithelial lymphocytes and Paneth cells, and attenuating the other elements investigated.
publishDate 2020
dc.date.none.fl_str_mv 2020-06-17
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Modelo animal, técnicas histológicas
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36925
10.5433/1679-0359.2020v41n5p1625
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36925
identifier_str_mv 10.5433/1679-0359.2020v41n5p1625
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36925/27528
dc.rights.driver.fl_str_mv Copyright (c) 2020 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 41 No. 5 (2020); 1625-1638
Semina: Ciências Agrárias; v. 41 n. 5 (2020); 1625-1638
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
_version_ 1799306081692811264

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