Milk contamination in different points of the dairy process. ii) mesophilic, psychrotrophic and proteolytic microorganisms

Detalhes bibliográficos
Autor(a) principal: Santana, Elsa Helena Walter de
Data de Publicação: 2004
Outros Autores: Beloti, Vanerli, Müller, Ernest Eckehardt, Ferreira, Márcia de Aguiar, Moraes, Luciane Bilia de, Pereira, Mykel Stepanni, Gusmão, Viviane Vieira
Tipo de documento: Artigo
Idioma: por
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2254
Resumo: Psychrotrophics microorganisms are able to multiply under refrigeration temperatures. They can produce heat-resistant extracellular enzymes that cause organoleptic and structural changes in milk and dairy products. With the objective of determining the frequency of microorganisms in the dairy process, four farms were evaluated in Londrina city – Brazil and different points were assessed. Mesophilic (48hs at 35oC), psychrotrophic (25hs at 21oC) and proteolytic (72hs at 22oC) microorganisms were researched, and the proteolytic predominant microflora were determined by using the Gram stain technique. The main psychrotrophic contamination points were the residual water on milk cans, on bulk tank and bad cleaned teats, and milk cans and teats were the most important sources of proteolytics. Gram negative bacilli represented 69% of the microorganisms in the refrigerated milk that means 45% of the total proteolytics microorganisms in the dairy process. Gram negative bacilli is most frequent on bad cleaned teats, teatcups, residual water in milk cans and bulk tank. The psychrotroph count in the refrigerated milk was more than the mesophilic count, showing that that psychrotrophics are the best indicatator for refrigerated milk contamination. The psycrotrophics count at all points assessed were greater than the ideal limit (10% of the mesophilic count). Thus as refrigeration at 4oC is not sufficient to control the multiplication of the psycrotrophics group, good practices should be associated to avoid or control milk contamination by psycrotrophics.
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spelling Milk contamination in different points of the dairy process. ii) mesophilic, psychrotrophic and proteolytic microorganismsContaminação do leite em diferentes pontos da produção leiteira: ii) microrganismos mesófilos, psicrotróficos e proteolíticosMilkQualityPsychrotrophicsProteolyticsRefrigeration.LeiteQualidadePsicrotróficosProteolíticosRefrigeração.Psychrotrophics microorganisms are able to multiply under refrigeration temperatures. They can produce heat-resistant extracellular enzymes that cause organoleptic and structural changes in milk and dairy products. With the objective of determining the frequency of microorganisms in the dairy process, four farms were evaluated in Londrina city – Brazil and different points were assessed. Mesophilic (48hs at 35oC), psychrotrophic (25hs at 21oC) and proteolytic (72hs at 22oC) microorganisms were researched, and the proteolytic predominant microflora were determined by using the Gram stain technique. The main psychrotrophic contamination points were the residual water on milk cans, on bulk tank and bad cleaned teats, and milk cans and teats were the most important sources of proteolytics. Gram negative bacilli represented 69% of the microorganisms in the refrigerated milk that means 45% of the total proteolytics microorganisms in the dairy process. Gram negative bacilli is most frequent on bad cleaned teats, teatcups, residual water in milk cans and bulk tank. The psychrotroph count in the refrigerated milk was more than the mesophilic count, showing that that psychrotrophics are the best indicatator for refrigerated milk contamination. The psycrotrophics count at all points assessed were greater than the ideal limit (10% of the mesophilic count). Thus as refrigeration at 4oC is not sufficient to control the multiplication of the psycrotrophics group, good practices should be associated to avoid or control milk contamination by psycrotrophics.Os psicrotróficos são microrganismos que têm capacidade de multiplicação em temperaturas de refrigeração. Produzem enzimas termorresistentes como proteases e lipases que promovem alterações organolépticas no leite e comprometem a produção de derivados. Com o objetivo de determinar a freqüência desses microrganismos a produção do leite, foram estudadas 04 propriedades da região de Londrina, PR, analisando diversos pontos do processo. Determinou-se a contagem de microrganismos aeróbios mesófilos (48hs a 35ºC), psicrotróficos (25hs a 21ºC), psicrotróficos proteolíticos (72 hs a 22ºC) e as características morfotintoriais da microbiota psicrotrófica proteolítica predominante. Os principais pontos de contaminação por psicrotróficos foram a água residual de latões, tanques de expansão e tetos higienizados inadequadamente, sendo que os psicrotróficos proteolíticos predominam nos latões e tetos. Bacilos Gram negativos representaram 45% da microbiota proteolítica de todo o processo de produção, no leite refrigerado corresponderam a 69% dos psicrotróficos proteolíticos encontrados e ainda predominaram nos tetos higienizados, teteiras, água residual de latões e tanques de expansão. As contagens de psicrotróficos superaram as de mesófilos em leite refrigerado, mostrando que a pesquisa de psicrotróficos é ideal para avaliar a real carga microbiana do leite refrigerado. Como a refrigeração a 4oC não inibe o crescimento deste grupo de microrganismos, deve-se evitar sua incorporação ao leite durante a produção, havendo necessidade da adoção de boas práticas de manejo em todo o processo produtivo.UEL2004-05-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/225410.5433/1679-0359.2004v25n4p349Semina: Ciências Agrárias; Vol. 25 No. 4 (2004); 349-358Semina: Ciências Agrárias; v. 25 n. 4 (2004); 349-3581679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2254/1934Santana, Elsa Helena Walter deBeloti, VanerliMüller, Ernest EckehardtFerreira, Márcia de AguiarMoraes, Luciane Bilia dePereira, Mykel StepanniGusmão, Viviane Vieirainfo:eu-repo/semantics/openAccess2010-03-17T17:49:01Zoai:ojs.pkp.sfu.ca:article/2254Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2010-03-17T17:49:01Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Milk contamination in different points of the dairy process. ii) mesophilic, psychrotrophic and proteolytic microorganisms
Contaminação do leite em diferentes pontos da produção leiteira: ii) microrganismos mesófilos, psicrotróficos e proteolíticos
title Milk contamination in different points of the dairy process. ii) mesophilic, psychrotrophic and proteolytic microorganisms
spellingShingle Milk contamination in different points of the dairy process. ii) mesophilic, psychrotrophic and proteolytic microorganisms
Santana, Elsa Helena Walter de
Milk
Quality
Psychrotrophics
Proteolytics
Refrigeration.
Leite
Qualidade
Psicrotróficos
Proteolíticos
Refrigeração.
title_short Milk contamination in different points of the dairy process. ii) mesophilic, psychrotrophic and proteolytic microorganisms
title_full Milk contamination in different points of the dairy process. ii) mesophilic, psychrotrophic and proteolytic microorganisms
title_fullStr Milk contamination in different points of the dairy process. ii) mesophilic, psychrotrophic and proteolytic microorganisms
title_full_unstemmed Milk contamination in different points of the dairy process. ii) mesophilic, psychrotrophic and proteolytic microorganisms
title_sort Milk contamination in different points of the dairy process. ii) mesophilic, psychrotrophic and proteolytic microorganisms
author Santana, Elsa Helena Walter de
author_facet Santana, Elsa Helena Walter de
Beloti, Vanerli
Müller, Ernest Eckehardt
Ferreira, Márcia de Aguiar
Moraes, Luciane Bilia de
Pereira, Mykel Stepanni
Gusmão, Viviane Vieira
author_role author
author2 Beloti, Vanerli
Müller, Ernest Eckehardt
Ferreira, Márcia de Aguiar
Moraes, Luciane Bilia de
Pereira, Mykel Stepanni
Gusmão, Viviane Vieira
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santana, Elsa Helena Walter de
Beloti, Vanerli
Müller, Ernest Eckehardt
Ferreira, Márcia de Aguiar
Moraes, Luciane Bilia de
Pereira, Mykel Stepanni
Gusmão, Viviane Vieira
dc.subject.por.fl_str_mv Milk
Quality
Psychrotrophics
Proteolytics
Refrigeration.
Leite
Qualidade
Psicrotróficos
Proteolíticos
Refrigeração.
topic Milk
Quality
Psychrotrophics
Proteolytics
Refrigeration.
Leite
Qualidade
Psicrotróficos
Proteolíticos
Refrigeração.
description Psychrotrophics microorganisms are able to multiply under refrigeration temperatures. They can produce heat-resistant extracellular enzymes that cause organoleptic and structural changes in milk and dairy products. With the objective of determining the frequency of microorganisms in the dairy process, four farms were evaluated in Londrina city – Brazil and different points were assessed. Mesophilic (48hs at 35oC), psychrotrophic (25hs at 21oC) and proteolytic (72hs at 22oC) microorganisms were researched, and the proteolytic predominant microflora were determined by using the Gram stain technique. The main psychrotrophic contamination points were the residual water on milk cans, on bulk tank and bad cleaned teats, and milk cans and teats were the most important sources of proteolytics. Gram negative bacilli represented 69% of the microorganisms in the refrigerated milk that means 45% of the total proteolytics microorganisms in the dairy process. Gram negative bacilli is most frequent on bad cleaned teats, teatcups, residual water in milk cans and bulk tank. The psychrotroph count in the refrigerated milk was more than the mesophilic count, showing that that psychrotrophics are the best indicatator for refrigerated milk contamination. The psycrotrophics count at all points assessed were greater than the ideal limit (10% of the mesophilic count). Thus as refrigeration at 4oC is not sufficient to control the multiplication of the psycrotrophics group, good practices should be associated to avoid or control milk contamination by psycrotrophics.
publishDate 2004
dc.date.none.fl_str_mv 2004-05-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2254
10.5433/1679-0359.2004v25n4p349
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2254
identifier_str_mv 10.5433/1679-0359.2004v25n4p349
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2254/1934
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 25 No. 4 (2004); 349-358
Semina: Ciências Agrárias; v. 25 n. 4 (2004); 349-358
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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