Risk factors associated with the occurrence of multiresistant Staphylococcus spp. isolated from bovine subclinical mastitis in northern Brazil

Detalhes bibliográficos
Autor(a) principal: Ferreira, Lilian Bernardina
Data de Publicação: 2022
Outros Autores: Israel, Larissa de Freitas Santiago, Rabello, Renata Fernandes, Souza, Guilherme Nunes de, Peruquetti, Rui Carlos, Medeiros, Luciana dos Santos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/44031
Resumo: Staphylococcus bacteria are often associated with subclinical bovine mastitis. This study aimed to identify multiresistant Staphylococcus spp. associated with subclinical mastitis and the associated risk factors. Twenty-three dairy farms with a history of decrease in milk production, located in the lower Acre region, Brazil, were selected. An epidemiological questionnaire was provided in all farms. All animals were examined using the California Mastitis Test (CMT) and their milk samples were collected for bacterial culture. After isolation and identification, the disk diffusion antimicrobial susceptibility test was performed against nine classes of antimicrobials. Of the 339 cows examined using the CMT, 108 had mastitis. A total of 229 milk samples were collected from individual teats. MALDI-TOF MS found isolates belonging to eight species of Staphylococcus, in 101 of these samples. S. chromogenes (58.4%) demonstrated strongest resistance to the nine classes of antimicrobial active principles. Nineteen isolates with multidrug resistance phenotypic profile were identified. This phenotypic expression indicates wide circulation of resistant genes in this species. The presence of multidrug resistance in Staphylococcus spp. in this study was correlated with lack of water for cleaning the corral, which is a preventive factor, minimizing the transmission and persistence of pathogens in the farms.
id UEL-11_70ce075d612eceb07395e7c830ab97d2
oai_identifier_str oai:ojs.pkp.sfu.ca:article/44031
network_acronym_str UEL-11
network_name_str Semina. Ciências Agrárias (Online)
repository_id_str
spelling Risk factors associated with the occurrence of multiresistant Staphylococcus spp. isolated from bovine subclinical mastitis in northern BrazilFatores de risco associados à ocorrência de espécies multirresistentes de Staphylococcus spp. isolados de mastite subclínica bovina na região norte do BrasilRisk factorsMastitisMultidrug resistanceStaphylococcus.Fatores de riscoMastiteMultirresistênciaStaphylococcus.Staphylococcus bacteria are often associated with subclinical bovine mastitis. This study aimed to identify multiresistant Staphylococcus spp. associated with subclinical mastitis and the associated risk factors. Twenty-three dairy farms with a history of decrease in milk production, located in the lower Acre region, Brazil, were selected. An epidemiological questionnaire was provided in all farms. All animals were examined using the California Mastitis Test (CMT) and their milk samples were collected for bacterial culture. After isolation and identification, the disk diffusion antimicrobial susceptibility test was performed against nine classes of antimicrobials. Of the 339 cows examined using the CMT, 108 had mastitis. A total of 229 milk samples were collected from individual teats. MALDI-TOF MS found isolates belonging to eight species of Staphylococcus, in 101 of these samples. S. chromogenes (58.4%) demonstrated strongest resistance to the nine classes of antimicrobial active principles. Nineteen isolates with multidrug resistance phenotypic profile were identified. This phenotypic expression indicates wide circulation of resistant genes in this species. The presence of multidrug resistance in Staphylococcus spp. in this study was correlated with lack of water for cleaning the corral, which is a preventive factor, minimizing the transmission and persistence of pathogens in the farms.Bactérias do gênero Staphylococcus são frequentemente associadas a mastite bovina subclínica. O objetivo do presente estudo foi identificar as espécies de Staphylococcus caracterizadas fenotipicamente como multirresistentes, associadas a mastite subclínica e identificar os fatores de risco associados. Foram selecionadas 23 propriedades leiteiras com histórico de queda de produção, localizadas na região do baixo Acre, Brasil. Em todas as propriedades foram preenchido um questionário epidemiológico. Todos os animais foram submetidos ao California Mastitis Test (CMT) e amostras de leite foram coletadas para cultura bacteriana. Após isolamento e identificação, foi realizado o teste de suscetibilidade antimicrobiana por difusão de disco frente à nove classes de antimicrobianos. Ao todo, 339 vacas foram testadas pelo CMT identificando 108 animais com mastite. Foram coletadas 229 amostras de leite de tetos individuais. Destas, 101 apresentaram isolados pertencentes ao gênero Staphylococcus, distribuídas em oito espécies identificadas por MALDI-TOF. S. chromogenes (58,4%) foi a espécie mais frequente demonstrando resistência as nove classes de princípios ativos antimicrobianos. Foram identificados 19 isolados com perfil fenotípico de multirresistência. Esta expressão fenotípica indica a ampla circulação de genes de resistência nesta espécie. A presença de multirresistência em Staphylococcus neste estudo foi correlacionada com a ausência da implementação do manejo em linha de ordenha e ausência de água no curral para limpeza, que funcionam como fatores preventivos, minimizando a transmissão e persistência dos patógenos nas propriedades.UEL2022-02-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4403110.5433/1679-0359.2022v43n2p901Semina: Ciências Agrárias; Vol. 43 No. 2 (2022); 901-910Semina: Ciências Agrárias; v. 43 n. 2 (2022); 901-9101679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/44031/30848Copyright (c) 2021 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessFerreira, Lilian BernardinaIsrael, Larissa de Freitas SantiagoRabello, Renata FernandesSouza, Guilherme Nunes dePeruquetti, Rui CarlosMedeiros, Luciana dos Santos2022-09-26T23:04:02Zoai:ojs.pkp.sfu.ca:article/44031Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-09-26T23:04:02Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Risk factors associated with the occurrence of multiresistant Staphylococcus spp. isolated from bovine subclinical mastitis in northern Brazil
Fatores de risco associados à ocorrência de espécies multirresistentes de Staphylococcus spp. isolados de mastite subclínica bovina na região norte do Brasil
title Risk factors associated with the occurrence of multiresistant Staphylococcus spp. isolated from bovine subclinical mastitis in northern Brazil
spellingShingle Risk factors associated with the occurrence of multiresistant Staphylococcus spp. isolated from bovine subclinical mastitis in northern Brazil
Ferreira, Lilian Bernardina
Risk factors
Mastitis
Multidrug resistance
Staphylococcus.
Fatores de risco
Mastite
Multirresistência
Staphylococcus.
title_short Risk factors associated with the occurrence of multiresistant Staphylococcus spp. isolated from bovine subclinical mastitis in northern Brazil
title_full Risk factors associated with the occurrence of multiresistant Staphylococcus spp. isolated from bovine subclinical mastitis in northern Brazil
title_fullStr Risk factors associated with the occurrence of multiresistant Staphylococcus spp. isolated from bovine subclinical mastitis in northern Brazil
title_full_unstemmed Risk factors associated with the occurrence of multiresistant Staphylococcus spp. isolated from bovine subclinical mastitis in northern Brazil
title_sort Risk factors associated with the occurrence of multiresistant Staphylococcus spp. isolated from bovine subclinical mastitis in northern Brazil
author Ferreira, Lilian Bernardina
author_facet Ferreira, Lilian Bernardina
Israel, Larissa de Freitas Santiago
Rabello, Renata Fernandes
Souza, Guilherme Nunes de
Peruquetti, Rui Carlos
Medeiros, Luciana dos Santos
author_role author
author2 Israel, Larissa de Freitas Santiago
Rabello, Renata Fernandes
Souza, Guilherme Nunes de
Peruquetti, Rui Carlos
Medeiros, Luciana dos Santos
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Ferreira, Lilian Bernardina
Israel, Larissa de Freitas Santiago
Rabello, Renata Fernandes
Souza, Guilherme Nunes de
Peruquetti, Rui Carlos
Medeiros, Luciana dos Santos
dc.subject.por.fl_str_mv Risk factors
Mastitis
Multidrug resistance
Staphylococcus.
Fatores de risco
Mastite
Multirresistência
Staphylococcus.
topic Risk factors
Mastitis
Multidrug resistance
Staphylococcus.
Fatores de risco
Mastite
Multirresistência
Staphylococcus.
description Staphylococcus bacteria are often associated with subclinical bovine mastitis. This study aimed to identify multiresistant Staphylococcus spp. associated with subclinical mastitis and the associated risk factors. Twenty-three dairy farms with a history of decrease in milk production, located in the lower Acre region, Brazil, were selected. An epidemiological questionnaire was provided in all farms. All animals were examined using the California Mastitis Test (CMT) and their milk samples were collected for bacterial culture. After isolation and identification, the disk diffusion antimicrobial susceptibility test was performed against nine classes of antimicrobials. Of the 339 cows examined using the CMT, 108 had mastitis. A total of 229 milk samples were collected from individual teats. MALDI-TOF MS found isolates belonging to eight species of Staphylococcus, in 101 of these samples. S. chromogenes (58.4%) demonstrated strongest resistance to the nine classes of antimicrobial active principles. Nineteen isolates with multidrug resistance phenotypic profile were identified. This phenotypic expression indicates wide circulation of resistant genes in this species. The presence of multidrug resistance in Staphylococcus spp. in this study was correlated with lack of water for cleaning the corral, which is a preventive factor, minimizing the transmission and persistence of pathogens in the farms.
publishDate 2022
dc.date.none.fl_str_mv 2022-02-25
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado por pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/44031
10.5433/1679-0359.2022v43n2p901
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/44031
identifier_str_mv 10.5433/1679-0359.2022v43n2p901
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/44031/30848
dc.rights.driver.fl_str_mv Copyright (c) 2021 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2021 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 43 No. 2 (2022); 901-910
Semina: Ciências Agrárias; v. 43 n. 2 (2022); 901-910
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
_version_ 1799306085583028224

Registros relacionados